Gene:
Gene Symbol:
CHMP2A
Gene Name:
charged multivesicular body protein 2A
Original Clone ID:
WISP12-101
Keyword:
Full length CHMP2A (aa 1-222)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCMV(delta3)
Format:
CLOSED
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
Authors:
University of Utah
Eiji Morita
Jun Arii
Devin Christensen
Jorg Votteler
Wesley I. Sundquist
Eiji Morita
Jun Arii
Devin Christensen
Jorg Votteler
Wesley I. Sundquist
HsCD00587819
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 669nts
Open reading frame: 1 to 669
ATGGACCTATTGTTCGGGCGCCGGAAGACGCCAGAGGAGCTACTGCGGCAGAACCAGAGG
GCCCTGAACCGTGCCATGCGGGAGCTGGACCGCGAGCGACAGAAACTAGAGACCCAGGAG
AAGAAAATCATTGCAGACATTAAGAAGATGGCCAAGCAAGGCCAGATGGATGCTGTTCGC
ATCATGGCAAAAGACTTGGTGCGCACCCGGCGCTATGTGCGCAAGTTTGTATTGATGCGG
GCCAACATCCAGGCTGTGTCCCTCAAGATCCAGACACTCAAGTCCAACAACTCGATGGCA
CAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAACAGACAGCTGAAGTTGCCC
CAGATCCAGAAGATCATGATGGAGTTTGAGCGGCAAGCTGAAATTATGGATATGAAGGAG
GAGATGATGAATGATGCCATTGATGATGCCATGGGTGATGAGGAAGACGAGGAAGAAAGT
GATGCTGTGGTGTCCCAGGTTCTGGATGAGCTGGGACTTAGCCTAACAGATGAGCTGTCG
AACCTCCCCTCAACTGGGGGCTCGCTTAGTGTGGCTGCTGGTGGGAAAAAAGCAGAGGCC
GCAGCCTCAGCCCTAGCTGATGCTGATGCAGACCTGGAGGAACGGCTTAAGAACCTGCGG
AGGGACTGA
Protein sequence predicted based on nucleotide sequence
MDLLFGRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKMAKQGQMDAVR IMAKDLVRTRRYVRKFVLMRANIQAVSLKIQTLKSNNSMAQAMKGVTKAMGTMNRQLKLP QIQKIMMEFERQAEIMDMKEEMMNDAIDDAMGDEEDEEESDAVVSQVLDELGLSLTDELS NLPSTGGSLSVAAGGKKAEAAASALADADADLEERLKNLRRD*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Mutations:
g396a; a399t; g402a; c405t;
t468c; a471g; g474a; g477a
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: neomycin
Host Type: mammalian Marker: neomycin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pCMV(delta3)
Synonyms:
WISP12-94
Description:
Mammalian expression vector with a deletion mutant of the CMV promoter; ampicillin resistance in bacteria, neomycin resistance in mammalian cells; restriction enzyme cloning
Comments:
Empty vector Sundquist Lab internal database number = WISP12-94. Restriction enzyme cloning can be used with this vector - e.g. Xho1, Kpn1 works well
Size (bp):
5027
Parent Vector:
Name:
pCMV(WT)
Description: Vector containing the full length CMV promoter
Properties:
mammalian expression, multiple cloning site
Author Name:
University of Utah
Wesley I. Sundquist
Eiji Morita
Jun Arii
Devin Christensen
Jorg Votteler
Wesley I. Sundquist
Eiji Morita
Jun Arii
Devin Christensen
Jorg Votteler
Publications:
PMID:
22877307
Title: Attenuated protein expression vectors for use in siRNA rescue experiments
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3254
3936
primer
forward primer
pcDNAF forward 5' sequencing primer AGAGAACCCACTGCTTACTGGCTTATC
615
638
primer
reverse primer
pcDNAR reverse 3' sequencing primer AACTAGAAGGCACAGTCGAGGCTG
380
406
promoter
CMV
truncated CMV promoter
234
464
selectable marker
AmpR
Ampicillin resistance
4034
4693
selectable marker
NeoR
Neomycin resistance (in mammalian cells)
1735
2526
ssDNA origin
f1
f1 origin
911
1217
