DNASU Plasmid | VPS16 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: VPS16

Gene Name: vacuolar protein sorting 16 homolog (S. cerevisiae)

Original Clone ID:

HR3434E-1-123-14.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00589051

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 372nts

Open reading frame: 31 to 402

Reference Sequence Alignment

ATGGGCCATCACCATCACCATCACAGCCATATGGACTGCTACACGGCGAACTGGAACCCA
CTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAG
GAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTG
AGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCT
GCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGC
TGGTCAGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGG
CTTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATTAG

Protein sequence predicted based on nucleotide sequence

MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAA
SVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFS
MGN*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR3434E

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Protein

Clone

Experimental

Organism

Genome
Reagents

Gene

Gene Symbol :

VPS16

Symbol Nomenclature :

VPS16

SYNONYM :

hVPS16

Designation :

vacuolar protein sorting protein 16|vacuolar protein sorting-associated protein 16 homolog

Full Nomenclature :

vacuolar protein sorting 16 homolog (S. cerevisiae)

GENEID :

64601

Locus Tag :

RP11-12M19.2

GenBank Accession :

NM_080414

HGNC :

14584

MIM :

608550

Vega :

OTTHUMG00000031714

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00589051      ATGGGCCATCACCATCACCATCACAGCCATATGGACTGCTACACGGCGAACTGGAACCCA
NM_022575.3       ------------------------------ATGGACTGCTACACGGCGAACTGGAACCCA
NM_080413.2       ------------------------------ATGGACTGCTACACGGCGAACTGGAACCCA
                                                ******************************

HsCD00589051      CTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAG
NM_022575.3       CTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAG
NM_080413.2       CTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAG
                  ************************************************************

HsCD00589051      GAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTG
NM_022575.3       GAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTG
NM_080413.2       GAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTG
                  ************************************************************

HsCD00589051      AGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCT
NM_022575.3       AGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCT
NM_080413.2       AGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCT
                  ************************************************************

HsCD00589051      GCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGC
NM_022575.3       GCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGC
NM_080413.2       GCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGC
                  ************************************************************

HsCD00589051      TGGTCAGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGG
NM_022575.3       TGGTCAGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGG
NM_080413.2       TGGTCAGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGG
                  ************************************************************

HsCD00589051      CTTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATTAG------------------
NM_022575.3       CTTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTT
NM_080413.2       CTTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTT
                  *************************************** *.                  

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CTGGATGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCC
NM_080413.2       CTGGATGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CACCGCTTCACCCTCAGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTG
NM_080413.2       CACCGCTTCACCCTCAGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CCAGGTCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACAC
NM_080413.2       CCAGGTCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACAC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       ATTCTTCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTG
NM_080413.2       ATTCTTCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       ACGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACC
NM_080413.2       ACGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       TACCGACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTC
NM_080413.2       TACCGACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       AAGGAGAAGCTATGTGAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGG
NM_080413.2       AAGGAGAAGCTATGTGAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       TGCAGCCGTCCTCGTAGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATG
NM_080413.2       TGCAGCCGTCCTCGTAGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       GTGGTGGGCGATGCACCCGAGAGCATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTG
NM_080413.2       GTGGTGGGCGATGCACCCGAGAGCATCCAATATAAGCAG---------------------
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CCTGAGCTCGATGGGGTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGTT
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CCAGCGGCCAGCGAGGAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTG
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       GAGGCTCAGAAGGAGTATGAGAAAGAGAGCCAGAAGGCGGACGAGTACCTGCGGGAGATC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CAGGAGCTGGGCCAGCTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAG
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CACCAGCCAGACATGCAGAAGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTG
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       GACAGATTTCCACCCGACAGCTTCGTGCACATGTGTCAGGACCTGCGTGTGCTCAATGCT
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       GTTCGGGACTATCACATCGGGATCCCGCTCACCTATAGCCAATATAAGCAGCTCACCATC
NM_080413.2       ---------------------------------------------------CTCACCATC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CAGGTGCTGCTGGACAGGCTCGTGTTGCGGAGACTTTACCCCCTGGCCATCCAGATATGC
NM_080413.2       CAGGTGCTGCTGGACAGGCTCGTGTTGCGGAGACTTTACCCCCTGGCCATCCAGATATGC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       GAGTACTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGC
NM_080413.2       GAGTACTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       TACAAGGTGCAACAGAAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAG
NM_080413.2       TACAAGGTGCAACAGAAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CTGGGGGACACGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGC
NM_080413.2       CTGGGGGACACGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CGCACGGAGCTGGCCATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCC
NM_080413.2       CGCACGGAGCTGGCCATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CTTCTCCTAAAGATGAAGAGGAGCAAACTGGCACTAAGCAAGGCCATCGAGAGCGGGGAC
NM_080413.2       CTTCTCCTAAAGATGAAGAGGAGCAAACTGGCACTAAGCAAGGCCATCGAGAGCGGGGAC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       ACTGACCTGGTGTTCACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTT
NM_080413.2       ACTGACCTGGTGTTCACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTT
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       TTCATGACCCTTCGGAATCAGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCAT
NM_080413.2       TTCATGACCCTTCGGAATCAGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCAT
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CAGGAGCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGGC
NM_080413.2       CAGGAGCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGGC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       AGCTTCCACATCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCT
NM_080413.2       AGCTTCCACATCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCT
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CTGCAGACAGCCGCCGATGCCTTCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACA
NM_080413.2       CTGCAGACAGCCGCCGATGCCTTCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACA
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       GAGGATCAAATGCGGCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAG
NM_080413.2       GAGGATCAAATGCGGCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       TTCCTAGACCTGTCTCTACATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAG
NM_080413.2       TTCCTAGACCTGTCTCTACATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CGTGCAGAGCAGCTGGCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAAG
NM_080413.2       CGTGCAGAGCAGCTGGCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAAG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       CTGACTGCCCTGGCAGATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAG
NM_080413.2       CTGACTGCCCTGGCAGATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       AAATCACCCATTGGCTACCTGCCTTTTGTGGAGATCTGCATGAAACAACATAACAAATAC
NM_080413.2       AAATCACCCATTGGCTACCTGCCTTTTGTGGAGATCTGCATGAAACAACATAACAAATAC
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       GAAGCCAAGAAGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTT
NM_080413.2       GAAGCCAAGAAGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTT
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       GTTGGCGATGTGGCTCAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTG
NM_080413.2       GTTGGCGATGTGGCTCAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTG
                                                                              

HsCD00589051      ------------------------------------------------------------
NM_022575.3       AGCCTCGTATTGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCAA
NM_080413.2       AGCCTCGTATTGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCAA
                                                                              

HsCD00589051      ------------------------------
NM_022575.3       CGGGCCAGGGCACAAGCCCAGAAGAAGTGA
NM_080413.2       CGGGCCAGGGCACAAGCCCAGAAGAAGTGA

Protein

Structural Annotation Wiki :

HR3434E

UniProt :

Q9H269

HPRD :

12259

PDBe Mol* Structure Viewer

Clone

Cloning Information :

627699

Original Clone ID :

HR3434E-1-123-14.3

Experimental

TargetTrack - Experimental data :

HR3434E

Organism

Species Specific ID:

64601

Genome

Chromosome :

Map Location :

20p13

Ensembl :

ENSG00000215305

Reagents

Labome :

VPS16-antibody

VPS16 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

PDBe Mol* Structure Viewer

Clone

Experimental

Organism

Genome

Reagents

VPS16 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)