DNASU Plasmid | VPS16 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: VPS16

Gene Name: vacuolar protein sorting 16 homolog (S. cerevisiae)

Original Clone ID:

HR3434E-9-129-14.5

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00589052

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 369nts

Open reading frame: 51 to 419

Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGAACCCAC
TCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGG
AGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGA
GGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTG
CTTCTGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCT
GGTCAGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGC
TTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGTAGC
TCGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG

Protein sequence predicted based on nucleotide sequence

MNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLD
IYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQ
NR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c195t,S65S

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR3434E

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Protein

Clone

Experimental

Organism

Genome
Reagents

Gene

Gene Symbol :

VPS16

Symbol Nomenclature :

VPS16

SYNONYM :

hVPS16

Designation :

vacuolar protein sorting protein 16|vacuolar protein sorting-associated protein 16 homolog

Full Nomenclature :

vacuolar protein sorting 16 homolog (S. cerevisiae)

GENEID :

64601

Locus Tag :

RP11-12M19.2

GenBank Accession :

NM_080414

HGNC :

14584

MIM :

608550

Vega :

OTTHUMG00000031714

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00589052      CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGAACCCAC
NM_022575.3       -----------------------------ATGGACTGCTACACGGCGAACTGGAACCCAC
NM_080413.2       -----------------------------ATGGACTGCTACACGGCGAACTGGAACCCAC
                                               .:  *  .* * .. .  .* : ********

HsCD00589052      TCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGG
NM_022575.3       TCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGG
NM_080413.2       TCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGG
                  ************************************************************

HsCD00589052      AGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGA
NM_022575.3       AGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGA
NM_080413.2       AGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGA
                  ************************************************************

HsCD00589052      GGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTG
NM_022575.3       GGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTG
NM_080413.2       GGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTG
                  ************************************************************

HsCD00589052      CTTCTGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCT
NM_022575.3       CTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCT
NM_080413.2       CTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCT
                  **** *******************************************************

HsCD00589052      GGTCAGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGC
NM_022575.3       GGTCAGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGC
NM_080413.2       GGTCAGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGC
                  ************************************************************

HsCD00589052      TTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGTAGC
NM_022575.3       TTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTC
NM_080413.2       TTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTC
                  ******************************************************** : *

HsCD00589052      TCGAGATCCG--------------------------------------------------
NM_022575.3       TGGATGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCC
NM_080413.2       TGGATGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCC
                  * ** . ***                                                  

HsCD00589052      --------------------------GCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
NM_022575.3       ACCGCTTCACCCTCAGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTGC
NM_080413.2       ACCGCTTCACCCTCAGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTGC
                                            * **. .:****  ***...*.:. *:*** ** 

HsCD00589052      CTGCTG------------------------------------------------------
NM_022575.3       CAGGTCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACA
NM_080413.2       CAGGTCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACA
                  *:* *                                                       

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TTCTTCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGA
NM_080413.2       TTCTTCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGA
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       CGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACCT
NM_080413.2       CGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACCT
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       ACCGACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCA
NM_080413.2       ACCGACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCA
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       AGGAGAAGCTATGTGAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGT
NM_080413.2       AGGAGAAGCTATGTGAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGT
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       GCAGCCGTCCTCGTAGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGG
NM_080413.2       GCAGCCGTCCTCGTAGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGG
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TGGTGGGCGATGCACCCGAGAGCATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTGC
NM_080413.2       TGGTGGGCGATGCACCCGAGAGCATCCAATATAAGCAG----------------------
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       CTGAGCTCGATGGGGTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGTTC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       CAGCGGCCAGCGAGGAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTGG
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       AGGCTCAGAAGGAGTATGAGAAAGAGAGCCAGAAGGCGGACGAGTACCTGCGGGAGATCC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       AGGAGCTGGGCCAGCTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       ACCAGCCAGACATGCAGAAGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTGG
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       ACAGATTTCCACCCGACAGCTTCGTGCACATGTGTCAGGACCTGCGTGTGCTCAATGCTG
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TTCGGGACTATCACATCGGGATCCCGCTCACCTATAGCCAATATAAGCAGCTCACCATCC
NM_080413.2       --------------------------------------------------CTCACCATCC
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       AGGTGCTGCTGGACAGGCTCGTGTTGCGGAGACTTTACCCCCTGGCCATCCAGATATGCG
NM_080413.2       AGGTGCTGCTGGACAGGCTCGTGTTGCGGAGACTTTACCCCCTGGCCATCCAGATATGCG
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       AGTACTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGCT
NM_080413.2       AGTACTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGCT
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       ACAAGGTGCAACAGAAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGC
NM_080413.2       ACAAGGTGCAACAGAAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGC
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TGGGGGACACGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGCC
NM_080413.2       TGGGGGACACGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGCC
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       GCACGGAGCTGGCCATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCC
NM_080413.2       GCACGGAGCTGGCCATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCC
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TTCTCCTAAAGATGAAGAGGAGCAAACTGGCACTAAGCAAGGCCATCGAGAGCGGGGACA
NM_080413.2       TTCTCCTAAAGATGAAGAGGAGCAAACTGGCACTAAGCAAGGCCATCGAGAGCGGGGACA
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       CTGACCTGGTGTTCACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTTT
NM_080413.2       CTGACCTGGTGTTCACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTTT
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TCATGACCCTTCGGAATCAGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCATC
NM_080413.2       TCATGACCCTTCGGAATCAGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCATC
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       AGGAGCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGGCA
NM_080413.2       AGGAGCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGGCA
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       GCTTCCACATCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCTC
NM_080413.2       GCTTCCACATCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCTC
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TGCAGACAGCCGCCGATGCCTTCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACAG
NM_080413.2       TGCAGACAGCCGCCGATGCCTTCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACAG
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       AGGATCAAATGCGGCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGT
NM_080413.2       AGGATCAAATGCGGCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGT
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TCCTAGACCTGTCTCTACATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGC
NM_080413.2       TCCTAGACCTGTCTCTACATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGC
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       GTGCAGAGCAGCTGGCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAAGC
NM_080413.2       GTGCAGAGCAGCTGGCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAAGC
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TGACTGCCCTGGCAGATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAGA
NM_080413.2       TGACTGCCCTGGCAGATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAGA
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       AATCACCCATTGGCTACCTGCCTTTTGTGGAGATCTGCATGAAACAACATAACAAATACG
NM_080413.2       AATCACCCATTGGCTACCTGCCTTTTGTGGAGATCTGCATGAAACAACATAACAAATACG
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       AAGCCAAGAAGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTTG
NM_080413.2       AAGCCAAGAAGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTTG
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       TTGGCGATGTGGCTCAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGA
NM_080413.2       TTGGCGATGTGGCTCAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGA
                                                                              

HsCD00589052      ------------------------------------------------------------
NM_022575.3       GCCTCGTATTGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCAAC
NM_080413.2       GCCTCGTATTGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCAAC
                                                                              

HsCD00589052      -----------------------------
NM_022575.3       GGGCCAGGGCACAAGCCCAGAAGAAGTGA
NM_080413.2       GGGCCAGGGCACAAGCCCAGAAGAAGTGA

Protein

Structural Annotation Wiki :

HR3434E

UniProt :

Q9H269

HPRD :

12259

PDBe Mol* Structure Viewer

Clone

Cloning Information :

627700

Original Clone ID :

HR3434E-9-129-14.5

Experimental

TargetTrack - Experimental data :

HR3434E

Organism

Species Specific ID:

64601

Genome

Chromosome :

Map Location :

20p13

Ensembl :

ENSG00000215305

Reagents

Labome :

VPS16-antibody

VPS16 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

PDBe Mol* Structure Viewer

Clone

Experimental

Organism

Genome

Reagents

VPS16 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)