DNASU Plasmid | RNGTT (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RNGTT

Gene Name: RNA guanylyltransferase and 5'-phosphatase

Original Clone ID:

HR6187A-229-567-14.21

Keyword:

full length protein truncation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI
Northeast Structural Genomics Consortium

HsCD00591512

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1023nts

Open reading frame: 19 to 1041

Reference Sequence Alignment

CATCACCATCACAGCCATATGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGTGTA
ACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTCTGT
GGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAGAATATT
AAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGGTACATG
ATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTTCAT
GTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACTCTC
TTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTGATA
TATGACATAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGTCTG
CAGTGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTCATT
GACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACTTCA
AGAAAGCTACTTGAAGGAAATTTTGCCAAAGAGGTGAGCCATGAAATGGATGGACTTATT
TTTCAGCCTACTGGAAAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAGCCT
CCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGGGTTA
CTTCCTCAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAAATC
AAGGTGACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAGAAC
AACAGCTGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAACACT
GCCATGGCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAGTTC
ATCGACAGATGTACTGCATAGCTCGAGATCCGGCTGCTA

Protein sequence predicted based on nucleotide sequence

MGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLK
PYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMI
IDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEP
FSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDILKWKPPSLNSV
DFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIECKFENNSWVFM
RQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTA*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

a156g,Q52Q;a615g,E205E

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in HR6187A

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

RNGTT

Symbol Nomenclature :

RNGTT

SYNONYM :

CAP1A; HCE; HCE1; hCAP

Designation :

HCAP1|mRNA-capping enzyme

Full Nomenclature :

RNA guanylyltransferase and 5'-phosphatase

GENEID :

8732

Locus Tag :

RP3-488C13.1

GI :

134142828

GenBank Accession :

NM_003800

HGNC :

10073

MIM :

603512

Vega :

OTTHUMG00000015190

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      ATGGCTCACAACAAGATCCCGCCGCGGTGGCTGAACTGTCCCCGGCGCGGCCAGCCGGTG
NM_003800.4         ATGGCTCACAACAAGATCCCGCCGCGGTGGCTGAACTGTCCCCGGCGCGGCCAGCCGGTG
NM_001286428.1      ------------------------------------------------------------
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      GCAGGAAGATTCTTACCTCTGAAGACAATGTTAGGACCAAGATATGATAGTCAAGTTGCT
NM_003800.4         GCAGGAAGATTCTTACCTCTGAAGACAATGTTAGGACCAAGATATGATAGTCAAGTTGCT
NM_001286428.1      ------------------------------------------------------------
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      GAAGAAAATCGGTTCCATCCCAGCATGCTCTCAAATTACCTAAAGAGCCTAAAGGTTAAA
NM_003800.4         GAAGAAAATCGGTTCCATCCCAGCATGCTCTCAAATTACCTAAAGAGCCTAAAGGTTAAA
NM_001286428.1      ------------------------------------------------------------
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      ATGGGCTTGTTGGTGGACCTGACAAATACTTCAAGGTTCTATGACCGAAATGACATAGAA
NM_003800.4         ATGGGCTTGTTGGTGGACCTGACAAATACTTCAAGGTTCTATGACCGAAATGACATAGAA
NM_001286428.1      ATGGGCTTGTTGGTGGACCTGACAAATACTTCAAGGTTCTATGACCGAAATGACATAGAA
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      AAAGAAGGAATCAAATATATAAAACTTCAGTGTAAAGGACATGGTGAGTGCCCTACCACT
NM_003800.4         AAAGAAGGAATCAAATATATAAAACTTCAGTGTAAAGGACATGGTGAGTGCCCTACCACT
NM_001286428.1      AAAGAAGGAATCAAATATATAAAACTTCAGTGTAAAGGACATGGTGAGTGCCCTACCACT
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      GAGAATACTGAGACCTTTATTCGTCTGTGTGAGCGGTTTAATGAAAGAAATCCACCTGAA
NM_003800.4         GAGAATACTGAGACCTTTATTCGTCTGTGTGAGCGGTTTAATGAAAGAAATCCACCTGAA
NM_001286428.1      GAGAATACTGAGACCTTTATTCGTCTGTGTGAGCGGTTTAATGAAAGAAATCCACCTGAA
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      CTTATAGGTGTTCATTGTACTCATGGCTTCAATCGCACTGGTTTCCTCATATGTGCCTTT
NM_003800.4         CTTATAGGTGTTCATTGTACTCATGGCTTCAATCGCACTGGTTTCCTCATATGTGCCTTT
NM_001286428.1      CTTATAGGTGTTCATTGTACTCATGGCTTCAATCGCACTGGTTTCCTCATATGTGCCTTT
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      TTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCAGTTGCTACTTTTGCCCAAGCCAGA
NM_003800.4         TTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCAGTTGCTACTTTTGCCCAAGCCAGA
NM_001286428.1      TTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCAGTTGCTACTTTTGCCCAAGCCAGA
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      CCACCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCGGTATGGTGACATA
NM_003800.4         CCACCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCGGTATGGTGACATA
NM_001286428.1      CCACCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCGGTATGGTGACATA
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      GAGGAAGCACCACCCCCACCTCTATTGCCAGATTGGTGTTTTGAGGATGATGAAGACGAA
NM_003800.4         GAGGAAGCACCACCCCCACCTCTATTGCCAGATTGGTGTTTTGAGGATGATGAAGACGAA
NM_001286428.1      GAGGAAGCACCACCCCCACCTCTATTGCCAGATTGGTGTTTTGAGGATGATGAAGACGAA
                                                                                

HsCD00591512        ------------------------------------------------------------
NM_001286426.1      GATGAGGATGAGGATGGAAAGAAGGAATCAGAACCCGGGTCAAGTGCTTCTTTTGGCAAA
NM_003800.4         GATGAGGATGAGGATGGAAAGAAGGAATCAGAACCCGGGTCAAGTGCTTCTTTTGGCAAA
NM_001286428.1      GATGAGGATGAGGATGGAAAGAAGGAATCAGAACCCGGGTCAAGTGCTTCTTTTGGCAAA
                                                                                

HsCD00591512        ---CATCACCATCACAGCCATATGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGT
NM_001286426.1      AGGAGAAAAGAACGGTTAAAACTGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGT
NM_003800.4         AGGAGAAAAGAACGGTTAAAACTGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGT
NM_001286428.1      AGGAGAAAAGAACGGTTAAAACTGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGT
                       ..:.*. *:*. : ..*:.**************************************

HsCD00591512        GTAACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTC
NM_001286426.1      GTAACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTC
NM_003800.4         GTAACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTC
NM_001286428.1      GTAACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTC
                    ************************************************************

HsCD00591512        TGTGGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAGAAT
NM_001286426.1      TGTGGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAAAAT
NM_003800.4         TGTGGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAAAAT
NM_001286428.1      TGTGGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAAAAT
                    ********************************************************.***

HsCD00591512        ATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGGTAC
NM_001286426.1      ATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGGTAC
NM_003800.4         ATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGGTAC
NM_001286428.1      ATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGGTAC
                    ************************************************************

HsCD00591512        ATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTT
NM_001286426.1      ATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTT
NM_003800.4         ATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTT
NM_001286428.1      ATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTT
                    ************************************************************

HsCD00591512        CATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACT
NM_001286426.1      CATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACT
NM_003800.4         CATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACT
NM_001286428.1      CATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACT
                    ************************************************************

HsCD00591512        CTCTTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTG
NM_001286426.1      CTCTTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTG
NM_003800.4         CTCTTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTG
NM_001286428.1      CTCTTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTG
                    ************************************************************

HsCD00591512        ATATATGACATAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGT
NM_001286426.1      ATATATGACATAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGT
NM_003800.4         ATATATGACATAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGT
NM_001286428.1      ATATATGACATAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGT
                    ************************************************************

HsCD00591512        CTGCAGTGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTC
NM_001286426.1      CTGCAGTGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTC
NM_003800.4         CTGCAGTGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTC
NM_001286428.1      CTGCAGTGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTC
                    ************************************************************

HsCD00591512        ATTGACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACT
NM_001286426.1      ATTGACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACT
NM_003800.4         ATTGACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACT
NM_001286428.1      ATTGACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACT
                    ************************************************************

HsCD00591512        TCAAGAAAGCTACTTGAAGGAAATTTTGCCAAAGAGGTGAGCCATGAAATGGATGGACTT
NM_001286426.1      TCAAGAAAG---------------------------------------------------
NM_003800.4         TCAAGAAAGCTACTTGAAGGAAATTTTGCCAAAGAAGTGAGCCATGAAATGGATGGACTT
NM_001286428.1      TCAAGAAAG---------------------------------------------------
                    *********                                                   

HsCD00591512        ATTTTTCAGCCTACTGGAAAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAG
NM_001286426.1      ------------------AAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAG
NM_003800.4         ATTTTTCAGCCTACTGGAAAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAG
NM_001286428.1      ------------------AAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAG
                                      ******************************************

HsCD00591512        CCTCCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGGG
NM_001286426.1      CCTCCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGGG
NM_003800.4         CCTCCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGGG
NM_001286428.1      CCTCCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGGG
                    ************************************************************

HsCD00591512        TTACTTCCTCAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAA
NM_001286426.1      TTACTTCCTCAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAA
NM_003800.4         TTACTTCCTCAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAA
NM_001286428.1      TTACTTCCTCAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAA
                    ************************************************************

HsCD00591512        ATCAAGGTGACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAG
NM_001286426.1      ATCAAGGTGACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAG
NM_003800.4         ATCAAGGTGACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAG
NM_001286428.1      ATCAAGGTGACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAG
                    ************************************************************

HsCD00591512        AACAACAGCTGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAAC
NM_001286426.1      AACAACAGCTGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAAC
NM_003800.4         AACAACAGCTGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAAC
NM_001286428.1      AACAACAGCTGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAAC
                    ************************************************************

HsCD00591512        ACTGCCATGGCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAG
NM_001286426.1      ACTGCCATGGCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAG
NM_003800.4         ACTGCCATGGCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAG
NM_001286428.1      ACTGCCATGGCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAG
                    ************************************************************

HsCD00591512        TTCATCGACAGATGTACTGCATAGCTCGAGATCCGGCTGCTA------------------
NM_001286426.1      TTCATCGACAGATGTACTGCAGCTTCTCAAGGACAGAAGCGAAAACATCATCTGGACCCT
NM_003800.4         TTCATCGACAGATGTACTGCAGCTTCTCAAGGACAGAAGCGAAAACATCATCTGGACCCT
NM_001286428.1      TTCATCGACAGATGTACTGCAGCTTCTCAAGGACAGAAGCGAAAACATCATCTGGACCCT
                    ********************* .     *.. .*.*.:** *                  

HsCD00591512        ------------------------------------------------------
NM_001286426.1      GACACGGAGCTCATGCCACCACCACCTCCCAAAAGACCACGCCCTTTAACCTAA
NM_003800.4         GACACGGAGCTCATGCCACCACCACCTCCCAAAAGACCACGCCCTTTAACCTAA
NM_001286428.1      GACACGGAGCTCATGCCACCACCACCTCCCAAAAGACCACGCCCTTTAACCTAA

Pathways

Reactome :

Disease

Reactome :

Gene Expression

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

mRNA Capping

Reactome :

RNA Pol II CTD phosphorylation and interaction with CE

Reactome :

RNA Polymerase II Transcription

Reactome :

Transcription

Reactome :

Transcription of the HIV genome

WikiPathway :

mRNA processing

Protein

Structural Annotation Wiki :

HR6187A

UniProt :

O60942

HPRD :

04621

Clone

Cloning Information :

534961

HIP Master Clone ID :

445281

Original Clone ID :

HR6187A-229-567-14.21

Experimental

TargetTrack - Experimental data :

HR6187A

Organism

Species Specific ID:

8732

Genome

Chromosome :

Map Location :

6q16

Ensembl :

ENSG00000111880

Reagents

Labome :

RNGTT-antibody

RNGTT (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

RNGTT (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)