DNASU Plasmid | VPS16 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: VPS16

Gene Name: vacuolar protein sorting 16 homolog (S. cerevisiae)

Original Clone ID:

HR3434G-1-414-15.1

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00596593

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1245nts

Open reading frame: 26 to 1270

Reference Sequence Alignment

CCATCACCATCACCATCACAGCCATATGGACTGCTACACGGCGAACTGGAACCCACTCGG
GGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGGAGGA
ACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGAGGAA
CCCCTGGTGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTGCTTC
CGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTC
AGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCA
TGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGA
TGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCG
CTTCACCCTCAGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGG
TCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACATTCT
TCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCC
CCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACCTACCG
ACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGA
GAAGCTATGTGAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAG
CCGTCCTCGTAGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGT
GGGCGATGCACCCGAGAACATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGA
GCTCGATGGGGTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGTTCCAGC
GGCCAGCGAGGAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGC
TCAGAAGGAGTATGAGAAAGAGAGCCAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGA
GCTGGGCCAGCTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCA
GCCAGACATGCAGAAGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAG
ATTTCCATAG

Protein sequence predicted based on nucleotide sequence

MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWWKEKAA
SVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFS
MGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCW
TVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDT
GYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPENIQ
FVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKES
QKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c163t,R55W;g893a,S298N

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in HR3434G

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Protein

Clone

Experimental

Organism

Genome
Reagents

Gene

Gene Symbol :

VPS16

Symbol Nomenclature :

VPS16

SYNONYM :

hVPS16

Designation :

vacuolar protein sorting protein 16|vacuolar protein sorting-associated protein 16 homolog

Full Nomenclature :

vacuolar protein sorting 16 homolog (S. cerevisiae)

GENEID :

64601

Locus Tag :

RP11-12M19.2

GenBank Accession :

NM_080414

HGNC :

14584

MIM :

608550

Vega :

OTTHUMG00000031714

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00596593      CCATCACCATCACCATCACAGCCATATGGACTGCTACACGGCGAACTGGAACCCACTCGG
NM_022575.3       -------------------------ATGGACTGCTACACGGCGAACTGGAACCCACTCGG
                                           ***********************************

HsCD00596593      GGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGGAGGA
NM_022575.3       GGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGGAGGA
                  ************************************************************

HsCD00596593      ACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGAGGAA
NM_022575.3       ACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGAGGAA
                  ************************************************************

HsCD00596593      CCCCTGGTGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTGCTTC
NM_022575.3       CCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTGCTTC
                  ******* ****************************************************

HsCD00596593      CGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTC
NM_022575.3       CGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTC
                  ************************************************************

HsCD00596593      AGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCA
NM_022575.3       AGCTGAGGAGGAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCA
                  ************************************************************

HsCD00596593      TGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGA
NM_022575.3       TGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGA
                  ************************************************************

HsCD00596593      TGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCG
NM_022575.3       TGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCG
                  ************************************************************

HsCD00596593      CTTCACCCTCAGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGG
NM_022575.3       CTTCACCCTCAGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGG
                  ************************************************************

HsCD00596593      TCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACATTCT
NM_022575.3       TCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACATTCT
                  ************************************************************

HsCD00596593      TCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCC
NM_022575.3       TCTGGCTGTGGGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCC
                  ************************************************************

HsCD00596593      CCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACCTACCG
NM_022575.3       CCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACCTACCG
                  ************************************************************

HsCD00596593      ACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGA
NM_022575.3       ACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGA
                  ************************************************************

HsCD00596593      GAAGCTATGTGAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAG
NM_022575.3       GAAGCTATGTGAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAG
                  ************************************************************

HsCD00596593      CCGTCCTCGTAGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGT
NM_022575.3       CCGTCCTCGTAGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGT
                  ************************************************************

HsCD00596593      GGGCGATGCACCCGAGAACATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGA
NM_022575.3       GGGCGATGCACCCGAGAGCATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGA
                  *****************.******************************************

HsCD00596593      GCTCGATGGGGTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGTTCCAGC
NM_022575.3       GCTCGATGGGGTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGTTCCAGC
                  ************************************************************

HsCD00596593      GGCCAGCGAGGAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGC
NM_022575.3       GGCCAGCGAGGAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGC
                  ************************************************************

HsCD00596593      TCAGAAGGAGTATGAGAAAGAGAGCCAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGA
NM_022575.3       TCAGAAGGAGTATGAGAAAGAGAGCCAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGA
                  ************************************************************

HsCD00596593      GCTGGGCCAGCTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCA
NM_022575.3       GCTGGGCCAGCTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCA
                  ************************************************************

HsCD00596593      GCCAGACATGCAGAAGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAG
NM_022575.3       GCCAGACATGCAGAAGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAG
                  ************************************************************

HsCD00596593      ATTTCCATAG--------------------------------------------------
NM_022575.3       ATTTCCACCCGACAGCTTCGTGCACATGTGTCAGGACCTGCGTGTGCTCAATGCTGTTCG
                  ******* .                                                   

HsCD00596593      ------------------------------------------------------------
NM_022575.3       GGACTATCACATCGGGATCCCGCTCACCTATAGCCAATATAAGCAGCTCACCATCCAGGT
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       GCTGCTGGACAGGCTCGTGTTGCGGAGACTTTACCCCCTGGCCATCCAGATATGCGAGTA
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       CTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAA
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       GGTGCAACAGAAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGG
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       GGACACGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGCCGCAC
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       GGAGCTGGCCATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCT
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       CCTAAAGATGAAGAGGAGCAAACTGGCACTAAGCAAGGCCATCGAGAGCGGGGACACTGA
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       CCTGGTGTTCACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTTTTCAT
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       GACCCTTCGGAATCAGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGA
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       GCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGGCAGCTT
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       CCACATCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCA
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       GACAGCCGCCGATGCCTTCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGA
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       TCAAATGCGGCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCT
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       AGACCTGTCTCTACATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGCGTGC
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       AGAGCAGCTGGCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGAC
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       TGCCCTGGCAGATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATC
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       ACCCATTGGCTACCTGCCTTTTGTGGAGATCTGCATGAAACAACATAACAAATACGAAGC
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       CAAGAAGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGG
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       CGATGTGGCTCAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGAGCCT
                                                                              

HsCD00596593      ------------------------------------------------------------
NM_022575.3       CGTATTGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCAACGGGC
                                                                              

HsCD00596593      -------------------------
NM_022575.3       CAGGGCACAAGCCCAGAAGAAGTGA

Protein

Structural Annotation Wiki :

HR3434G

UniProt :

Q9H269

HPRD :

12259

PDBe Mol* Structure Viewer

Clone

Cloning Information :

629049

Original Clone ID :

HR3434G-1-414-15.1

Experimental

TargetTrack - Experimental data :

HR3434G

Organism

Species Specific ID:

64601

Genome

Chromosome :

Map Location :

20p13

Ensembl :

ENSG00000215305

Reagents

Labome :

VPS16-antibody

VPS16 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

PDBe Mol* Structure Viewer

Clone

Experimental

Organism

Genome

Reagents

VPS16 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)