DNASU Plasmid | VPS16 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: VPS16

Gene Name: vacuolar protein sorting 16 homolog (S. cerevisiae)

Original Clone ID:

HR3434G-1-419-15.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00596594

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1260nts

Open reading frame: 16 to 1275

Reference Sequence Alignment

CACCATCACAGCCATATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCC
TTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGGAGGAACTCAGGGAT
TGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGG
AAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTGCTTCCGGCATGCCT
CTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCCGGTCAGCTGAGGAG
GAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTC
CGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATC
TTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTC
AGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGT
GCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTG
GGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTG
GCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACCTACCGACACCTGGCA
CTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGT
GAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGT
AGCAAGGAGAGGGCCGAGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCA
CCCGAGAGCATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGAGCTCGATGGG
GTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGTTCCAGCGGCCAGCGAG
GAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGCTCAGAAGGAG
TATGAGAAAGAGAGCCAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGAGCTGGGCCAG
CTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATG
CAGAAGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCC
GACAGCTTCGTGTAG

Protein sequence predicted based on nucleotide sequence

MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAA
SVRPVLDIYSASGMPLASLLWKSGPVVSLGRSAEEELLCVQEDGAVLVYGLHGDFRRHFS
MGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCW
TVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDT
GYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAEVVAWERRLMVVGDAPESIQ
FVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKES
QKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFV*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t271c,W91R;t842a,V281E

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in HR3434G

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Protein

Clone

Experimental

Organism

Genome
Reagents

Gene

Gene Symbol :

VPS16

Symbol Nomenclature :

VPS16

SYNONYM :

hVPS16

Designation :

vacuolar protein sorting protein 16|vacuolar protein sorting-associated protein 16 homolog

Full Nomenclature :

vacuolar protein sorting 16 homolog (S. cerevisiae)

GENEID :

64601

Locus Tag :

RP11-12M19.2

GenBank Accession :

NM_080414

HGNC :

14584

MIM :

608550

Vega :

OTTHUMG00000031714

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00596594      CACCATCACAGCCATATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCC
NM_022575.3       ---------------ATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCC
                                 *********************************************

HsCD00596594      TTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGGAGGAACTCAGGGAT
NM_022575.3       TTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAAGGAGGAACTCAGGGAT
                  ************************************************************

HsCD00596594      TGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGG
NM_022575.3       TGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGG
                  ************************************************************

HsCD00596594      AAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTGCTTCCGGCATGCCT
NM_022575.3       AAGGAGAAAGCTGCTAGTGTGAGGCCAGTGCTCGATATATACTCTGCTTCCGGCATGCCT
                  ************************************************************

HsCD00596594      CTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCCGGTCAGCTGAGGAG
NM_022575.3       CTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCAGCTGAGGAG
                  ********************************************* **************

HsCD00596594      GAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTC
NM_022575.3       GAGCTGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTC
                  ************************************************************

HsCD00596594      CGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATC
NM_022575.3       CGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATC
                  ************************************************************

HsCD00596594      TTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTC
NM_022575.3       TTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTC
                  ************************************************************

HsCD00596594      AGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGT
NM_022575.3       AGTGCCAATGTGGGTGACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGT
                  ************************************************************

HsCD00596594      GCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTG
NM_022575.3       GCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTG
                  ************************************************************

HsCD00596594      GGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTG
NM_022575.3       GGGCCTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTG
                  ************************************************************

HsCD00596594      GCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACCTACCGACACCTGGCA
NM_022575.3       GCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCACCTACCGACACCTGGCA
                  ************************************************************

HsCD00596594      CTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGT
NM_022575.3       CTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGT
                  ************************************************************

HsCD00596594      GAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGT
NM_022575.3       GAGTTCAACTGCAACATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGT
                  ************************************************************

HsCD00596594      AGCAAGGAGAGGGCCGAGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCA
NM_022575.3       AGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCA
                  ****************:*******************************************

HsCD00596594      CCCGAGAGCATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGAGCTCGATGGG
NM_022575.3       CCCGAGAGCATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGAGCTCGATGGG
                  ************************************************************

HsCD00596594      GTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGTTCCAGCGGCCAGCGAG
NM_022575.3       GTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGTTCCAGCGGCCAGCGAG
                  ************************************************************

HsCD00596594      GAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGCTCAGAAGGAG
NM_022575.3       GAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGCTCAGAAGGAG
                  ************************************************************

HsCD00596594      TATGAGAAAGAGAGCCAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGAGCTGGGCCAG
NM_022575.3       TATGAGAAAGAGAGCCAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGAGCTGGGCCAG
                  ************************************************************

HsCD00596594      CTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATG
NM_022575.3       CTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATG
                  ************************************************************

HsCD00596594      CAGAAGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCC
NM_022575.3       CAGAAGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCC
                  ************************************************************

HsCD00596594      GACAGCTTCGTGTAG---------------------------------------------
NM_022575.3       GACAGCTTCGTGCACATGTGTCAGGACCTGCGTGTGCTCAATGCTGTTCGGGACTATCAC
                  ************ *                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       ATCGGGATCCCGCTCACCTATAGCCAATATAAGCAGCTCACCATCCAGGTGCTGCTGGAC
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       AGGCTCGTGTTGCGGAGACTTTACCCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTT
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       CCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAG
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       AAGGATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCT
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       GGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCC
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       ATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATG
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       AAGAGGAGCAAACTGGCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTC
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       ACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGG
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       AATCAGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACG
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       CTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATCCGA
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       GCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCC
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       GATGCCTTCTACAAGGCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGG
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       CTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCT
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       CTACATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTG
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       GCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCA
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       GATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGC
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       TACCTGCCTTTTGTGGAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAGTAT
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       GCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCT
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       CAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCC
                                                                              

HsCD00596594      ------------------------------------------------------------
NM_022575.3       CACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCACAA
                                                                              

HsCD00596594      ---------------
NM_022575.3       GCCCAGAAGAAGTGA

Protein

Structural Annotation Wiki :

HR3434G

UniProt :

Q9H269

HPRD :

12259

PDBe Mol* Structure Viewer

Clone

Cloning Information :

629050

Original Clone ID :

HR3434G-1-419-15.3

Experimental

TargetTrack - Experimental data :

HR3434G

Organism

Species Specific ID:

64601

Genome

Chromosome :

Map Location :

20p13

Ensembl :

ENSG00000215305

Reagents

Labome :

VPS16-antibody

VPS16 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

PDBe Mol* Structure Viewer

Clone

Experimental

Organism

Genome

Reagents

VPS16 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)