DNASU Plasmid | VPS16 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: VPS16

Gene Name: vacuolar protein sorting 16 homolog (S. cerevisiae)

Original Clone ID:

HR3434H-512-828-15.1

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00596596

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 957nts

Open reading frame: 51 to 1007

Reference Sequence Alignment

TTTAAGAAGGAGATATACCATGGGGCCATCACCATCACCATCACAGCCATATGGGGGACA
CGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGC
TGGCCATCAAGCTGCTTGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAA
AGATGAAGAGGAGCAAACAGGCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGG
TGTTCGCGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCC
TTCGGAATCAGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAG
AGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACA
TCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAG
CCGCCGATGCCTTCTACAAGGCTAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAA
TGCGTCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACC
TGTCTCTACATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGC
AGCTGGCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCC
TGGCAGATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCA
TTGGCTACCTGCCTTTTGTGGAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGA
AGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGATCAAGGCTTTGCTTCTTGTTGGCGATG
TGGCTCAGGCTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTAT
TGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGTAGCTCGAGATCCGGC
TGCTAACAAAGCCCGAAAGGAA

Protein sequence predicted based on nucleotide sequence

MGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKQALSKAIESGD
TDLVFAVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELG
SFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQ
FLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSK
KSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKIKALLLVGDVAQAADVAIEHRNEAEL
SLVLSHCTGATDGATADK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g87t,L29L;t149a,L50Q;

a196g,T66A;c453t,A151A;

g495t,R165R;g823a,V275I

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR3434H

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Protein

Clone

Experimental

Organism

Genome
Reagents

Gene

Gene Symbol :

VPS16

Symbol Nomenclature :

VPS16

SYNONYM :

hVPS16

Designation :

vacuolar protein sorting protein 16|vacuolar protein sorting-associated protein 16 homolog

Full Nomenclature :

vacuolar protein sorting 16 homolog (S. cerevisiae)

GENEID :

64601

Locus Tag :

RP11-12M19.2

GenBank Accession :

NM_080414

HGNC :

14584

MIM :

608550

Vega :

OTTHUMG00000031714

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00596596      ------------------------------------------------------------
NM_022575.3       ATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATAT
NM_080413.2       ATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATAT
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       GAGCTGTACAGCATGGACTGGGACCTGAAGGAGGAACTCAGGGATTGCCTGGTGGCTGCT
NM_080413.2       GAGCTGTACAGCATGGACTGGGACCTGAAGGAGGAACTCAGGGATTGCCTGGTGGCTGCT
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       GCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCT
NM_080413.2       GCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCT
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       AGTGTGAGGCCAGTGCTCGATATATACTCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTG
NM_080413.2       AGTGTGAGGCCAGTGCTCGATATATACTCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTG
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       TGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCAGCTGAGGAGGAGCTGCTCTGTGTG
NM_080413.2       TGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCAGCTGAGGAGGAGCTGCTCTGTGTG
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       CAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTCCGGAGACACTTCAGC
NM_080413.2       CAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTCCGGAGACACTTCAGC
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       ATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATCTTTCACACTGAGTTT
NM_080413.2       ATGGGCAATGAAGTGCTCCAGAACCGGGTTCTGGATGCCCGGATCTTTCACACTGAGTTT
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       GGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGT
NM_080413.2       GGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGT
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       GACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGG
NM_080413.2       GACCTCAAACTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGG
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       ACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTGGGGCCTGACCTTTAC
NM_080413.2       ACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTGGGGCCTGACCTTTAC
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       CTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGC
NM_080413.2       CTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGC
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       AGCTTCCTACAGATGGCTGTCTCCTTCACCTACCGACACCTGGCACTCTTCACAGACACA
NM_080413.2       AGCTTCCTACAGATGGCTGTCTCCTTCACCTACCGACACCTGGCACTCTTCACAGACACA
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       GGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGTGAGTTCAACTGCAAC
NM_080413.2       GGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGTGAGTTCAACTGCAAC
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       ATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGCAAGGAGAGGGCC
NM_080413.2       ATCCGGGCACCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGCAAGGAGAGGGCC
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       GTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCGAGAGCATCCAG
NM_080413.2       GTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCGAGAGCATCCAA
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       TTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGAGCTCGATGGGGTCCGCATCTTCTCC
NM_080413.2       TATAAGCAG---------------------------------------------------
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       CGCAGCACCCACGAGTTCCTGCATGAGGTTCCAGCGGCCAGCGAGGAAATCTTCAAAATT
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       GCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGCTCAGAAGGAGTATGAGAAAGAGAGC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       CAGAAGGCGGACGAGTACCTGCGGGAGATCCAGGAGCTGGGCCAGCTGACCCAGGCCGTG
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       CAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATGCAGAAGAGTCTGCTC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       AGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCCGACAGCTTCGTGCAC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       ATGTGTCAGGACCTGCGTGTGCTCAATGCTGTTCGGGACTATCACATCGGGATCCCGCTC
NM_080413.2       ------------------------------------------------------------
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       ACCTATAGCCAATATAAGCAGCTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGG
NM_080413.2       ---------------------CTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGG
                                                                              

HsCD00596596      ------------------------------------------------------------
NM_022575.3       AGACTTTACCCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGC
NM_080413.2       AGACTTTACCCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGC
                                                                              

HsCD00596596      ----------------------------------------TTTAAGAAGGAGATATACCA
NM_022575.3       GTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAGAAGGATGTCTCAGAT
NM_080413.2       GTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAGAAGGATGTCTCAGAT
                                                          :: *..*.*.:*: : * .:

HsCD00596596      TGGGGCCATCACCATCACCATCACAGCCATATGGGGGACACGCCTGGTGTCTCTTACTCC
NM_022575.3       GAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCTGGTGTCTCTTACTCC
NM_080413.2       GAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCTGGTGTCTCTTACTCC
                   .**.  :  . *.: .*.:*.**.. .* .*****************************

HsCD00596596      GACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCCATCAAGCTGCTTGAG
NM_022575.3       GACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCCATCAAGCTGCTGGAG
NM_080413.2       GACATTGCTGCACGAGCCTATGGTTGTGGCCGCACGGAGCTGGCCATCAAGCTGCTGGAG
                  ******************************************************** ***

HsCD00596596      TATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACAG
NM_022575.3       TATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACTG
NM_080413.2       TATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACTG
                  **********************************************************:*

HsCD00596596      GCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCGCGGTGTTGCTGCAC
NM_022575.3       GCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCAC
NM_080413.2       GCACTAAGCAAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCAC
                  *********************************************.**************

HsCD00596596      CTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCC
NM_022575.3       CTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCC
NM_080413.2       CTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATCAGCCCATGGCC
                  ************************************************************

HsCD00596596      CTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTAC
NM_022575.3       CTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTAC
NM_080413.2       CTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTAC
                  ************************************************************

HsCD00596596      AATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATCCGAGCCAGCTATGCTGCA
NM_022575.3       AATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATCCGAGCCAGCTATGCTGCA
NM_080413.2       AATCAGGATGACAATCACCAGGAATTGGGCAGCTTCCACATCCGAGCCAGCTATGCTGCA
                  ************************************************************

HsCD00596596      GAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCTTCTACAAG
NM_022575.3       GAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCTTCTACAAG
NM_080413.2       GAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCTTCTACAAG
                  ************************************************************

HsCD00596596      GCTAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGTCTCCTACGGCTGCAG
NM_022575.3       GCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCAG
NM_080413.2       GCCAAGAATGAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCAG
                  ** ***************************************** ***************

HsCD00596596      CGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTACATGACACAGTT
NM_022575.3       CGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTACATGACACAGTT
NM_080413.2       CGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTACATGACACAGTT
                  ************************************************************

HsCD00596596      ACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGC
NM_022575.3       ACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGC
NM_080413.2       ACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGC
                  ************************************************************

HsCD00596596      ATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGG
NM_022575.3       ATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGG
NM_080413.2       ATCCCTGACAAGAGGCTCTGGTGGCTGAAGCTGACTGCCCTGGCAGATTTGGAAGATTGG
                  ************************************************************

HsCD00596596      GAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTG
NM_022575.3       GAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTG
NM_080413.2       GAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTG
                  ************************************************************

HsCD00596596      GAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGT
NM_022575.3       GAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGT
NM_080413.2       GAGATCTGCATGAAACAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGT
                  ************************************************************

HsCD00596596      CCCGAGCAGAAGATCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGGCTGCAGATGTG
NM_022575.3       CCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGGCTGCAGATGTG
NM_080413.2       CCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGGCTGCAGATGTG
                  ************.***********************************************

HsCD00596596      GCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCC
NM_022575.3       GCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCC
NM_080413.2       GCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCC
                  ************************************************************

HsCD00596596      ACAGATGGGGCCACAGCTGACAAGTAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAAGG
NM_022575.3       ACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCACAAGCCCAGAAGAAGTGA
NM_080413.2       ACAGATGGGGCCACAGCTGACAAGATTCAACGGGCCAGGGCACAAGCCCAGAAGAAGTGA
                  ************************:: *:. .*. *.**  .*:*.*..**.. .*.:*.

HsCD00596596      AA
NM_022575.3       --
NM_080413.2       --

Protein

Structural Annotation Wiki :

HR3434H

UniProt :

Q9H269

HPRD :

12259

PDBe Mol* Structure Viewer

Clone

Cloning Information :

629053

Original Clone ID :

HR3434H-512-828-15.1

Experimental

TargetTrack - Experimental data :

HR3434H

Organism

Species Specific ID:

64601

Genome

Chromosome :

Map Location :

20p13

Ensembl :

ENSG00000215305

Reagents

Labome :

VPS16-antibody

VPS16 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

PDBe Mol* Structure Viewer

Clone

Experimental

Organism

Genome

Reagents

VPS16 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)