DNASU Plasmid | NSMAF (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: NSMAF

Gene Name: neutral sphingomyelinase (N-SMase) activation associated factor

Original Clone ID:

HR6313B-268-585-15.17

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00596879

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 960nts

Open reading frame: 51 to 1010

Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGATCTACC
TAAAGTTCTATGAACCTCAAGATAGAGATGATCTCTATTTTTACATTGCCACATACCTAG
AGCACCATGTGGCGGAGCACACTGCTGAGAGCTACATGCTGCAGTGGCAGCGTGGACACC
TTTCCAACTATCAGTACCTCCTTCACCTCAACAACCTGGCCGACCGCAGCTGCAACGACC
TCTCCCAGTACCCTGTGTTTCCATGGATAATACATGATTATTCCAGCTCAGAACTAGATT
TGTCAAATCCAGGAACCTTCCGGGATCTCAGTAAGCCAGTAGGGGCCCTAAATAAGGAAC
GGCTGGAGAGACTACTGACACGCTACCAGGAAATGCCTGAACCAAAGTTCATGTATGGGA
GTCACTACTCTTCCCCGGGTTATGTACTTTTTTATCTTGTTAGGATTGCACCAGAGTATA
TGCTGTGCCTGCAGAATGGAAGATTTGATAATGCAGATAGAATGTTCAACAGTATTGCAG
AAACTTGGAAAAACTGTCTGGATGGTGCAACGGATTTTAAAGAGTTAATTCCAGAATTCT
ATGGTGATGATGTAAGCTTTCTAGTCAATAGCCTGAAGTTGGATTTGGGAAAGAGACAAG
GAGGACAGATGGTTGACGATGTGGAGCTTCCCCCTTGGGCTTCCAGTCCCGAGGACTTTC
TCCAGAAGAGCAAAGATGCATTGGAAAGCAATTATGTGTCTGAACACCTTCACGAGTGGA
TTGATCTAATATTTGGCTACAAACAAAAAGGGAGTGATGCAGTTGGGGCCCATAATGTAT
TTCATCCCCTGACCTATGAAGGAGGTGTAGACTTGAACAGCATCCAGGATCCTGATGAGA
AGGTAGCCATGCTTACGCAAATCTTGGAATTTGGGCAGACACCAAAACAACTATTTGTGA
CACCACATCCTCGAAGGATCACCCCAAAGTTTAAAAGTTTGTCCCAGTAGCTCGAGATCC
GGCTGCTAACAAAGCCCGAAAGG

Protein sequence predicted based on nucleotide sequence

MIYLKFYEPQDRDDLYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHLNNLADRS
CNDLSQYPVFPWIIHDYSSSELDLSNPGTFRDLSKPVGALNKERLERLLTRYQEMPEPKF
MYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIAETWKNCLDGATDFKELI
PEFYGDDVSFLVNSLKLDLGKRQGGQMVDDVELPPWASSPEDFLQKSKDALESNYVSEHL
HEWIDLIFGYKQKGSDAVGAHNVFHPLTYEGGVDLNSIQDPDEKVAMLTQILEFGQTPKQ
LFVTPHPRRITPKFKSLSQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g564a,V188V

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR6313B

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

NSMAF

Symbol Nomenclature :

NSMAF

SYNONYM :

FAN

Designation :

factor associated with N-SMase activation|factor associated with neutral sphingomyelinase activation|protein FAN

Full Nomenclature :

neutral sphingomyelinase (N-SMase) activation associated factor

GENEID :

8439

GI :

31543297

GenBank Accession :

NM_003580

HGNC :

8017

MIM :

603043

Vega :

OTTHUMG00000164352

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      ATGACGGGCCAGGCCAGATTCCACCGCGGAGCCGAGGCATGCCGAGCTATCCGGCAGTGC
NM_003580.3         ------------------------------------------------------------
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AGGTCCCGGCGGCCGCGGGCAGCTCACCCGGAAGGCCGCGCGGGGTCCGGGCGTGGATCC
NM_003580.3         ----------------ATGGCGTTTATCCGGAAGAAGCAGCAGGAGCAGCAGCTG-----
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      CGGCACGCGAGCCCGGGCGTTCGCAGGGGAGGATTTTCCTTGCTGCTGCTTAACTTGGAG
NM_003580.3         ------------CAGCTCTACTCCAAGGAGAGATTTTCCTTGCTGCTGCTTAACTTGGAG
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      GAGTACTACTTTGAACAGCATAGAGCCAATCACATTTTGCACAAGGGCAGTCACCATGAA
NM_003580.3         GAGTACTACTTTGAACAGCATAGAGCCAATCACATTTTGCACAAGGGCAGTCACCATGAA
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AGGAAAATCAGAGGCTCCTTAAAAATATGTTCAAAATCGGTGATTTTTGAACCAGATTCA
NM_003580.3         AGGAAAATCAGAGGCTCCTTAAAAATATGTTCAAAATCGGTGATTTTTGAACCAGATTCA
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      ATATCCCAGCCCATCATCAAGATTCCTTTGAGAGACTGTATAAAAATAGGAAAGCATGGA
NM_003580.3         ATATCCCAGCCCATCATCAAGATTCCTTTGAGAGACTGTATAAAAATAGGAAAGCATGGA
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      GAAAATGGAGCCAATAGACACTTCACAAAGGCAAAATCTGGGGGTATTTCACTCATTTTC
NM_003580.3         GAAAATGGAGCCAATAGACACTTCACAAAGGCAAAATCTGGGGGTATTTCACTCATTTTC
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AGTCAGGTATATTTCATTAAAGAACATAATGTTGTTGCACCATATAAAATAGAAAGGGGC
NM_003580.3         AGTCAGGTATATTTCATTAAAGAACATAATGTTGTTGCACCATATAAAATAGAAAGGGGC
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AAAATGGAATATGTTTTTGAATTGGATGTTCCCGGGAAAGTGGAAGATGTTGTGGAGACG
NM_003580.3         AAAATGGAATATGTTTTTGAATTGGATGTTCCCGGGAAAGTGGAAGATGTTGTGGAGACG
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      TTGCTTCAGCTTCACAGAGCATCCTGCCTTGACAAATTGGGTGACCAAACCGCCATGATA
NM_003580.3         TTGCTTCAGCTTCACAGAGCATCCTGCCTTGACAAATTGGGTGACCAAACCGCCATGATA
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      ACAGCTATTTTGCAGTCTCGTTTAGCTAGAACATCATTTGACAAAAACAGGTTCCAAAAC
NM_003580.3         ACAGCTATTTTGCAGTCTCGTTTAGCTAGAACATCATTTGACAAAAACAGGTTCCAAAAC
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      ATTTCTGAAAAGCTGCACATGGAATGCAAAGCAGAAATGGTGACGCCTCTGGTGACTAAT
NM_003580.3         ATTTCTGAAAAGCTGCACATGGAATGCAAAGCAGAAATGGTGACGCCTCTGGTGACTAAT
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      CCTGGACACGTGTGCATCACGGACACAAACCTGTATTTTCAGCCCCTCAACGGCTACCCG
NM_003580.3         CCTGGACACGTGTGCATCACGGACACAAACCTGTATTTTCAGCCCCTCAACGGCTACCCG
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AAACCTGTGGTCCAGATAACACTCCAAGATGTCCGCCGCATCTACAAAAGGAGGCACGGC
NM_003580.3         AAACCTGTGGTCCAGATAACACTCCAAGATGTCCGCCGCATCTACAAAAGGAGGCACGGC
                                                                                

HsCD00596879        -CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGATCTAC
NM_001144772.1      CTCATGCCTCTGGGCTTGGAAGTATTTTGCACAGAAGATGATCTGTGTTCCGACATCTAC
NM_003580.3         CTCATGCCTCTGGGCTTGGAAGTATTTTGCACAGAAGATGATCTGTGTTCCGACATCTAC
                       :*.. :. *.*.*: ....*.    . ... *: *  ***: :*  . .: ******

HsCD00596879        CTAAAGTTCTATGAACCTCAAGATAGAGATGATCTCTATTTTTACATTGCCACATACCTA
NM_001144772.1      CTAAAGTTCTATGAACCTCAAGATAGAGATGATCTCTATTTTTACATTGCCACATACCTA
NM_003580.3         CTAAAGTTCTATGAACCTCAAGATAGAGATGATCTCTATTTTTACATTGCCACATACCTA
                    ************************************************************

HsCD00596879        GAGCACCATGTGGCGGAGCACACTGCTGAGAGCTACATGCTGCAGTGGCAGCGTGGACAC
NM_001144772.1      GAGCACCATGTGGCGGAGCACACTGCTGAGAGCTACATGCTGCAGTGGCAGCGTGGACAC
NM_003580.3         GAGCACCATGTGGCGGAGCACACTGCTGAGAGCTACATGCTGCAGTGGCAGCGTGGACAC
                    ************************************************************

HsCD00596879        CTTTCCAACTATCAGTACCTCCTTCACCTCAACAACCTGGCCGACCGCAGCTGCAACGAC
NM_001144772.1      CTTTCCAACTATCAGTACCTCCTTCACCTCAACAACCTGGCCGACCGCAGCTGCAACGAC
NM_003580.3         CTTTCCAACTATCAGTACCTCCTTCACCTCAACAACCTGGCCGACCGCAGCTGCAACGAC
                    ************************************************************

HsCD00596879        CTCTCCCAGTACCCTGTGTTTCCATGGATAATACATGATTATTCCAGCTCAGAACTAGAT
NM_001144772.1      CTCTCCCAGTACCCTGTGTTTCCATGGATAATACATGATTATTCCAGCTCAGAACTAGAT
NM_003580.3         CTCTCCCAGTACCCTGTGTTTCCATGGATAATACATGATTATTCCAGCTCAGAACTAGAT
                    ************************************************************

HsCD00596879        TTGTCAAATCCAGGAACCTTCCGGGATCTCAGTAAGCCAGTAGGGGCCCTAAATAAGGAA
NM_001144772.1      TTGTCAAATCCAGGAACCTTCCGGGATCTCAGTAAGCCAGTAGGGGCCCTAAATAAGGAA
NM_003580.3         TTGTCAAATCCAGGAACCTTCCGGGATCTCAGTAAGCCAGTAGGGGCCCTAAATAAGGAA
                    ************************************************************

HsCD00596879        CGGCTGGAGAGACTACTGACACGCTACCAGGAAATGCCTGAACCAAAGTTCATGTATGGG
NM_001144772.1      CGGCTGGAGAGACTACTGACACGCTACCAGGAAATGCCTGAACCAAAGTTCATGTATGGG
NM_003580.3         CGGCTGGAGAGACTACTGACACGCTACCAGGAAATGCCTGAACCAAAGTTCATGTATGGG
                    ************************************************************

HsCD00596879        AGTCACTACTCTTCCCCGGGTTATGTACTTTTTTATCTTGTTAGGATTGCACCAGAGTAT
NM_001144772.1      AGTCACTACTCTTCCCCGGGTTATGTACTTTTTTATCTTGTTAGGATTGCACCAGAGTAT
NM_003580.3         AGTCACTACTCTTCCCCGGGTTATGTACTTTTTTATCTTGTTAGGATTGCACCAGAGTAT
                    ************************************************************

HsCD00596879        ATGCTGTGCCTGCAGAATGGAAGATTTGATAATGCAGATAGAATGTTCAACAGTATTGCA
NM_001144772.1      ATGCTGTGCCTGCAGAATGGAAGATTTGATAATGCAGATAGAATGTTCAACAGTATTGCA
NM_003580.3         ATGCTGTGCCTGCAGAATGGAAGATTTGATAATGCAGATAGAATGTTCAACAGTATTGCA
                    ************************************************************

HsCD00596879        GAAACTTGGAAAAACTGTCTGGATGGTGCAACGGATTTTAAAGAGTTAATTCCAGAATTC
NM_001144772.1      GAAACTTGGAAAAACTGTCTGGATGGTGCAACGGATTTTAAAGAGTTAATTCCAGAATTC
NM_003580.3         GAAACTTGGAAAAACTGTCTGGATGGTGCAACGGATTTTAAAGAGTTAATTCCAGAATTC
                    ************************************************************

HsCD00596879        TATGGTGATGATGTAAGCTTTCTAGTCAATAGCCTGAAGTTGGATTTGGGAAAGAGACAA
NM_001144772.1      TATGGTGATGATGTGAGCTTTCTAGTCAATAGCCTGAAGTTGGATTTGGGAAAGAGACAA
NM_003580.3         TATGGTGATGATGTGAGCTTTCTAGTCAATAGCCTGAAGTTGGATTTGGGAAAGAGACAA
                    **************.*********************************************

HsCD00596879        GGAGGACAGATGGTTGACGATGTGGAGCTTCCCCCTTGGGCTTCCAGTCCCGAGGACTTT
NM_001144772.1      GGAGGACAGATGGTTGACGACGTGGAGCTTCCCCCTTGGGCTTCCAGTCCCGAGGACTTT
NM_003580.3         GGAGGACAGATGGTTGACGACGTGGAGCTTCCCCCTTGGGCTTCCAGTCCCGAGGACTTT
                    ******************** ***************************************

HsCD00596879        CTCCAGAAGAGCAAAGATGCATTGGAAAGCAATTATGTGTCTGAACACCTTCACGAGTGG
NM_001144772.1      CTCCAGAAGAGCAAAGATGCATTGGAAAGCAATTATGTGTCTGAACACCTTCACGAGTGG
NM_003580.3         CTCCAGAAGAGCAAAGATGCATTGGAAAGCAATTATGTGTCTGAACACCTTCACGAGTGG
                    ************************************************************

HsCD00596879        ATTGATCTAATATTTGGCTACAAACAAAAAGGGAGTGATGCAGTTGGGGCCCATAATGTA
NM_001144772.1      ATTGATCTAATATTTGGCTACAAACAAAAAGGGAGTGATGCAGTTGGGGCCCATAATGTA
NM_003580.3         ATTGATCTAATATTTGGCTACAAACAAAAAGGGAGTGATGCAGTTGGGGCCCATAATGTA
                    ************************************************************

HsCD00596879        TTTCATCCCCTGACCTATGAAGGAGGTGTAGACTTGAACAGCATCCAGGATCCTGATGAG
NM_001144772.1      TTTCATCCCCTGACCTATGAAGGAGGTGTAGACTTGAACAGCATCCAGGATCCTGATGAG
NM_003580.3         TTTCATCCCCTGACCTATGAAGGAGGTGTAGACTTGAACAGCATCCAGGATCCTGATGAG
                    ************************************************************

HsCD00596879        AAGGTAGCCATGCTTACGCAAATCTTGGAATTTGGGCAGACACCAAAACAACTATTTGTG
NM_001144772.1      AAGGTAGCCATGCTTACGCAAATCTTGGAATTTGGGCAGACACCAAAACAACTATTTGTG
NM_003580.3         AAGGTAGCCATGCTTACGCAAATCTTGGAATTTGGGCAGACACCAAAACAACTATTTGTG
                    ************************************************************

HsCD00596879        ACACCACATCCTCGAAGGATCACCCCAAAGTTTAAAAGTTTGTCCCAGTAGCTCG-----
NM_001144772.1      ACACCACATCCTCGAAGGATCACCCCAAAGTTTAAAAGTTTGTCCCAGACCTCCAGTTAT
NM_003580.3         ACACCACATCCTCGAAGGATCACCCCAAAGTTTAAAAGTTTGTCCCAGACCTCCAGTTAT
                    ************************************************:.   *.     

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AATGCTTCTATGGCAGATTCCCCAGGTGAAGAGTCTTTTGAAGACCTGACCGAAGAAAGC
NM_003580.3         AATGCTTCTATGGCAGATTCCCCAGGTGAAGAGTCTTTTGAAGACCTGACCGAAGAAAGC
                                                                                

HsCD00596879        ------------------------------------AGATCCGGCTGCTAACAAAGCCCG
NM_001144772.1      AAAACACTGGCCTGGAATAACATCACCAAACTGCAGTTACACGAGCACTATAAAATCCAC
NM_003580.3         AAAACACTGGCCTGGAATAACATCACCAAACTGCAGTTACACGAGCACTATAAAATCCAC
                                                        : * .**.  .***:.*** **. 

HsCD00596879        AAAGG-------------------------------------------------------
NM_001144772.1      AAAGAAGCAGTTACTGGAATCACGGTCTCTCGCAATGGATCTTCAGTATTCACAACATCC
NM_003580.3         AAAGAAGCAGTTACTGGAATCACGGTCTCTCGCAATGGATCTTCAGTATTCACAACATCC
                    ****.                                                       

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      CAAGATTCCACCTTGAAGATGTTTTCTAAAGAATCAAAAATGCTACAAAGAAGTATATCA
NM_003580.3         CAAGATTCCACCTTGAAGATGTTTTCTAAAGAATCAAAAATGCTACAAAGAAGTATATCA
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      TTTTCAAATATGGCTTTATCGTCTTGTTTACTTTTACCAGGAGATGCCACTGTCATAACT
NM_003580.3         TTTTCAAATATGGCTTTATCGTCTTGTTTACTTTTACCAGGAGATGCCACTGTCATAACT
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      TCTTCATGGGATAATAATGTCTATTTTTATTCCATAGCATTTGGAAGACGCCAGGACACG
NM_003580.3         TCTTCATGGGATAATAATGTCTATTTTTATTCCATAGCATTTGGAAGACGCCAGGACACG
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      TTAATGGGACATGATGATGCTGTTAGTAAGATCTGTTGGCATGACAACAGGCTATATTCT
NM_003580.3         TTAATGGGACATGATGATGCTGTTAGTAAGATCTGTTGGCATGACAACAGGCTATATTCT
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      GCATCGTGGGACTCTACAGTGAAGGTGTGGTCTGGTGTTCCTGCAGAGATGCCAGGCACC
NM_003580.3         GCATCGTGGGACTCTACAGTGAAGGTGTGGTCTGGTGTTCCTGCAGAGATGCCAGGCACC
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AAAAGACACCACTTTGACTTGCTGGCCGAGCTGGAACATGATGTCAGTGTAGATACAATC
NM_003580.3         AAAAGACACCACTTTGACTTGCTGGCCGAGCTGGAACATGATGTCAGTGTAGATACAATC
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AGTTTAAATGCTGCAAGCACACTGTTAGTTTCCGGCACCAAAGAAGGCACAGTGAATATT
NM_003580.3         AGTTTAAATGCTGCAAGCACACTGTTAGTTTCCGGCACCAAAGAAGGCACAGTGAATATT
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      TGGGACCTCACAACGGCCACCTTAATGCACCAGATTCCATGCCATTCAGGGATTGTATGT
NM_003580.3         TGGGACCTCACAACGGCCACCTTAATGCACCAGATTCCATGCCATTCAGGGATTGTATGT
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      GACACTGCTTTTAGCCCAGATAGTCGCCATGTCCTCAGCACAGGAACAGATGGCTGTCTT
NM_003580.3         GACACTGCTTTTAGCCCAGATAGTCGCCATGTCCTCAGCACAGGAACAGATGGCTGTCTT
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AATGTCATTGATGTGCAGACAGGAATGCTCATCTCCTCCATGACATCAGATGAGCCCCAG
NM_003580.3         AATGTCATTGATGTGCAGACAGGAATGCTCATCTCCTCCATGACATCAGATGAGCCCCAG
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      AGGTGCTTTGTCTGGGATGGAAATTCCGTTTTATCTGGCAGTCAGTCTGGTGAACTGCTC
NM_003580.3         AGGTGCTTTGTCTGGGATGGAAATTCCGTTTTATCTGGCAGTCAGTCTGGTGAACTGCTC
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      GTTTGGGACCTCCTTGGAGCAAAAATCAGTGAGAGAATACAGGGCCACACAGGTGCTGTG
NM_003580.3         GTTTGGGACCTCCTTGGAGCAAAAATCAGTGAGAGAATACAGGGCCACACAGGTGCTGTG
                                                                                

HsCD00596879        ------------------------------------------------------------
NM_001144772.1      ACATGTATATGGATGAATGAACAGTGTAGCAGTATCATCACAGGAGGGGAAGACAGACAA
NM_003580.3         ACATGTATATGGATGAATGAACAGTGTAGCAGTATCATCACAGGAGGGGAAGACAGACAA
                                                                                

HsCD00596879        ---------------------------
NM_001144772.1      ATTATATTCTGGAAATTGCAGTATTAA
NM_003580.3         ATTATATTCTGGAAATTGCAGTATTAA

Pathways

NCI :

Ceramide signaling pathway

NCI :

TNF receptor signaling pathway

WikiPathway :

TNF alpha Signaling Pathway

Protein

Structural Annotation Wiki :

HR6313B

UniProt :

Q92636

HPRD :

09117

Clone

Cloning Information :

627170

Original Clone ID :

HR6313B-268-585-15.17

Experimental

TargetTrack - Experimental data :

HR6313B

Organism

Species Specific ID:

8439

Genome

Chromosome :

Map Location :

8q12-q13

Ensembl :

ENSG00000035681

Reagents

Labome :

FAN-antibody

NSMAF (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

NSMAF (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)