DNASU Plasmid | SMARCB1 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: SMARCB1

Gene Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Original Clone ID:

HR6367-1-356-14.14

Keyword:

full length protein truncation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00597155

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1071nts

Open reading frame: 26 to 1096

Reference Sequence Alignment

CCATCACCATCACCATCACAGCCATATGATGATGATGGCGCTGAGCAAGACCTTCGGGCA
GAAGCCCGTGAAGTTCCAGCTGGAGGACGACGGCGAGTTCTACATGATCGGCTCCGAGGT
GGGAAACTACCTCCGTATGTTCCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAG
GCGACTAGCCACTGTGGAAGAGAGGAAGAAAATAGTTGCATCGTCACATGGTAAAAAAAC
AAAACCTAACACTAAGGATCACGGATACACGACTCTAGCCACCAGTGTAACCCTGTTAAA
AGCCTCGGAAGTGGAAGAGATTCTGGATGACAACGATGAGAAGTACAAGGCTGTGTCCAT
CAGCACAGAGCCCCCCACCTACCTCAGGGAACAGAAGGCCAAGAGGAACAGCCAGTGGGT
ACCCACCCTGCCCAACAGCTCCCACCACTTAGATGCCGTGCCATGCTCCACAACCATCAA
CAGGAACCGCATGGGCCGAGACAAGAAGAGAACCTTCCCCCTTTGCTTTGATGACCATGA
CCCAGCTGTGATCCATGAGAACGCATCTCAGCCCGAGGTGCTGGTCCCCATCCGGCTGGA
CATGGAGATCGATGGGCAGAAGCTGCGAGACGCCTTCACCTGGAACATGAATGAGAAGTT
GATGACGCCTGAGATGTTTTCAGAAATCCTCTGTGACGATCTGGATTTGAACCCGCTGAC
GTTTGTGCCAGCCATCGCCTCTGCCATCAGACAGCAGATCGAGTCCTACCCCACGGACAG
CATCCTGGAGGACCAGTCAGACCAGCGCGTCATCATCAAGCTGAACATCCATGTGGGAAA
CATTTCCCTGGTGGACCAGTTTGAGTGGGACATGTCAGAGAAGGAGAACTCACCAGAGAA
GTGTGCCCTGAAGCTGTGTTCGGAGTTGGGGTTGGGCGGGGAGTTTGTCACCACCATCGC
ATACAGCATCCGGGGACAGCTGAGCTGGCATCAGAAGACCTACGCCTTCAGCGAGAACCC
TCTGCCCACAGTGGAGATTGCCATCCGGAACACGGGCGATGCGGACCAGTGGTGCCCACT
GCTGGAGACTCTGTAG

Protein sequence predicted based on nucleotide sequence

MMMMALSKTFGQKPVKFQLEDDGEFYMIGSEVGNYLRMFRGSLYKRYPSLWRRLATVEER
KKIVASSHGKKTKPNTKDHGYTTLATSVTLLKASEVEEILDDNDEKYKAVSISTEPPTYL
REQKAKRNSQWVPTLPNSSHHLDAVPCSTTINRNRMGRDKKRTFPLCFDDHDPAVIHENA
SQPEVLVPIRLDMEIDGQKLRDAFTWNMNEKLMTPEMFSEILCDDLDLNPLTFVPAIASA
IRQQIESYPTDSILEDQSDQRVIIKLNIHVGNISLVDQFEWDMSEKENSPEKCALKLCSE
LGLGGEFVTTIAYSIRGQLSWHQKTYAFSENPLPTVEIAIRNTGDADQWCPLLETL*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g264a,V88V;g305a,G102D;

t878g,F293C;c894t,C298C;

c901t,L301L

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in HR6367

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

SMARCB1

Symbol Nomenclature :

SMARCB1

SYNONYM :

BAF47; INI1; MRD15; RDT; RTPS1; SNF5; SNF5L1; Sfh1p; Snr1; hSNFS

Designation :

Full Nomenclature :

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

GENEID :

6598

GI :

27545326

GenBank Accession :

NM_003073

HGNC :

11103

MIM :

601607

Vega :

OTTHUMG00000150737

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00597155        CCATCACCATCACCATCACAGCCATATGATGATGATGGCGCTGAGCAAGACCTTCGGGCA
NM_003073.3         -------------------------ATGATGATGATGGCGCTGAGCAAGACCTTCGGGCA
NM_001007468.1      -------------------------ATGATGATGATGGCGCTGAGCAAGACCTTCGGGCA
                                             ***********************************

HsCD00597155        GAAGCCCGTGAAGTTCCAGCTGGAGGACGACGGCGAGTTCTACATGATCGGCTCCGAGGT
NM_003073.3         GAAGCCCGTGAAGTTCCAGCTGGAGGACGACGGCGAGTTCTACATGATCGGCTCCGAGGT
NM_001007468.1      GAAGCCCGTGAAGTTCCAGCTGGAGGACGACGGCGAGTTCTACATGATCGGCTCCGAGGT
                    ************************************************************

HsCD00597155        GGGAAACTACCTCCGTATGTTCCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAG
NM_003073.3         GGGAAACTACCTCCGTATGTTCCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAG
NM_001007468.1      GGGAAACTACCTCCGTATGTTCCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAG
                    ************************************************************

HsCD00597155        GCGACTAGCCACTGTGGAAGAGAGGAAGAAAATAGTTGCATCGTCACATGGTAAAAAAAC
NM_003073.3         GCGACTAGCCACTGTGGAAGAGAGGAAGAAAATAGTTGCATCGTCACATGGTAAAAAAAC
NM_001007468.1      GCGACTAGCCACTGTGGAAGAGAGGAAGAAAATAGTTGCATCGTCACATG----------
                    **************************************************          

HsCD00597155        AAAACCTAACACTAAGGATCACGGATACACGACTCTAGCCACCAGTGTAACCCTGTTAAA
NM_003073.3         AAAACCTAACACTAAGGATCACGGATACACGACTCTAGCCACCAGTGTGACCCTGTTAAA
NM_001007468.1      -----------------ATCACGGATACACGACTCTAGCCACCAGTGTGACCCTGTTAAA
                                     *******************************.***********

HsCD00597155        AGCCTCGGAAGTGGAAGAGATTCTGGATGACAACGATGAGAAGTACAAGGCTGTGTCCAT
NM_003073.3         AGCCTCGGAAGTGGAAGAGATTCTGGATGGCAACGATGAGAAGTACAAGGCTGTGTCCAT
NM_001007468.1      AGCCTCGGAAGTGGAAGAGATTCTGGATGGCAACGATGAGAAGTACAAGGCTGTGTCCAT
                    *****************************.******************************

HsCD00597155        CAGCACAGAGCCCCCCACCTACCTCAGGGAACAGAAGGCCAAGAGGAACAGCCAGTGGGT
NM_003073.3         CAGCACAGAGCCCCCCACCTACCTCAGGGAACAGAAGGCCAAGAGGAACAGCCAGTGGGT
NM_001007468.1      CAGCACAGAGCCCCCCACCTACCTCAGGGAACAGAAGGCCAAGAGGAACAGCCAGTGGGT
                    ************************************************************

HsCD00597155        ACCCACCCTGCCCAACAGCTCCCACCACTTAGATGCCGTGCCATGCTCCACAACCATCAA
NM_003073.3         ACCCACCCTGCCCAACAGCTCCCACCACTTAGATGCCGTGCCATGCTCCACAACCATCAA
NM_001007468.1      ACCCACCCTGCCCAACAGCTCCCACCACTTAGATGCCGTGCCATGCTCCACAACCATCAA
                    ************************************************************

HsCD00597155        CAGGAACCGCATGGGCCGAGACAAGAAGAGAACCTTCCCCCTTTGCTTTGATGACCATGA
NM_003073.3         CAGGAACCGCATGGGCCGAGACAAGAAGAGAACCTTCCCCCTTTGCTTTGATGACCATGA
NM_001007468.1      CAGGAACCGCATGGGCCGAGACAAGAAGAGAACCTTCCCCCTTTGCTTTGATGACCATGA
                    ************************************************************

HsCD00597155        CCCAGCTGTGATCCATGAGAACGCATCTCAGCCCGAGGTGCTGGTCCCCATCCGGCTGGA
NM_003073.3         CCCAGCTGTGATCCATGAGAACGCATCTCAGCCCGAGGTGCTGGTCCCCATCCGGCTGGA
NM_001007468.1      CCCAGCTGTGATCCATGAGAACGCATCTCAGCCCGAGGTGCTGGTCCCCATCCGGCTGGA
                    ************************************************************

HsCD00597155        CATGGAGATCGATGGGCAGAAGCTGCGAGACGCCTTCACCTGGAACATGAATGAGAAGTT
NM_003073.3         CATGGAGATCGATGGGCAGAAGCTGCGAGACGCCTTCACCTGGAACATGAATGAGAAGTT
NM_001007468.1      CATGGAGATCGATGGGCAGAAGCTGCGAGACGCCTTCACCTGGAACATGAATGAGAAGTT
                    ************************************************************

HsCD00597155        GATGACGCCTGAGATGTTTTCAGAAATCCTCTGTGACGATCTGGATTTGAACCCGCTGAC
NM_003073.3         GATGACGCCTGAGATGTTTTCAGAAATCCTCTGTGACGATCTGGATTTGAACCCGCTGAC
NM_001007468.1      GATGACGCCTGAGATGTTTTCAGAAATCCTCTGTGACGATCTGGATTTGAACCCGCTGAC
                    ************************************************************

HsCD00597155        GTTTGTGCCAGCCATCGCCTCTGCCATCAGACAGCAGATCGAGTCCTACCCCACGGACAG
NM_003073.3         GTTTGTGCCAGCCATCGCCTCTGCCATCAGACAGCAGATCGAGTCCTACCCCACGGACAG
NM_001007468.1      GTTTGTGCCAGCCATCGCCTCTGCCATCAGACAGCAGATCGAGTCCTACCCCACGGACAG
                    ************************************************************

HsCD00597155        CATCCTGGAGGACCAGTCAGACCAGCGCGTCATCATCAAGCTGAACATCCATGTGGGAAA
NM_003073.3         CATCCTGGAGGACCAGTCAGACCAGCGCGTCATCATCAAGCTGAACATCCATGTGGGAAA
NM_001007468.1      CATCCTGGAGGACCAGTCAGACCAGCGCGTCATCATCAAGCTGAACATCCATGTGGGAAA
                    ************************************************************

HsCD00597155        CATTTCCCTGGTGGACCAGTTTGAGTGGGACATGTCAGAGAAGGAGAACTCACCAGAGAA
NM_003073.3         CATTTCCCTGGTGGACCAGTTTGAGTGGGACATGTCAGAGAAGGAGAACTCACCAGAGAA
NM_001007468.1      CATTTCCCTGGTGGACCAGTTTGAGTGGGACATGTCAGAGAAGGAGAACTCACCAGAGAA
                    ************************************************************

HsCD00597155        GTGTGCCCTGAAGCTGTGTTCGGAGTTGGGGTTGGGCGGGGAGTTTGTCACCACCATCGC
NM_003073.3         GTTTGCCCTGAAGCTGTGCTCGGAGCTGGGGTTGGGCGGGGAGTTTGTCACCACCATCGC
NM_001007468.1      GTTTGCCCTGAAGCTGTGCTCGGAGCTGGGGTTGGGCGGGGAGTTTGTCACCACCATCGC
                    ** *************** ****** **********************************

HsCD00597155        ATACAGCATCCGGGGACAGCTGAGCTGGCATCAGAAGACCTACGCCTTCAGCGAGAACCC
NM_003073.3         ATACAGCATCCGGGGACAGCTGAGCTGGCATCAGAAGACCTACGCCTTCAGCGAGAACCC
NM_001007468.1      ATACAGCATCCGGGGACAGCTGAGCTGGCATCAGAAGACCTACGCCTTCAGCGAGAACCC
                    ************************************************************

HsCD00597155        TCTGCCCACAGTGGAGATTGCCATCCGGAACACGGGCGATGCGGACCAGTGGTGCCCACT
NM_003073.3         TCTGCCCACAGTGGAGATTGCCATCCGGAACACGGGCGATGCGGACCAGTGGTGCCCACT
NM_001007468.1      TCTGCCCACAGTGGAGATTGCCATCCGGAACACGGGCGATGCGGACCAGTGGTGCCCACT
                    ************************************************************

HsCD00597155        GCTGGAGACTCTGTAG--------------------------------------------
NM_003073.3         GCTGGAGACTCTGACAGACGCTGAGATGGAGAAGAAGATCCGCGACCAGGACAGGAACAC
NM_001007468.1      GCTGGAGACTCTGACAGACGCTGAGATGGAGAAGAAGATCCGCGACCAGGACAGGAACAC
                    *************:..                                            

HsCD00597155        -------------------------------------------
NM_003073.3         GAGGCGGATGAGGCGTCTTGCCAACACGGCCCCGGCCTGGTAA
NM_001007468.1      GAGGCGGATGAGGCGTCTTGCCAACACGGCCCCGGCCTGGTAA

Pathways

NCI :

Regulation of retinoblastoma protein

NCI :

Validated nuclear estrogen receptor beta network

Panther :

Wnt signaling pathway

Protein

Structural Annotation Wiki :

HR6367

UniProt :

Q12824

HPRD :

03364

Clone

Cloning Information :

627285

Original Clone ID :

HR6367-1-356-14.14

Experimental

TargetTrack - Experimental data :

HR6367

Organism

Species Specific ID:

6598

Genome

Chromosome :

Map Location :

22q11.23|22q11

Ensembl :

ENSG00000099956

Reagents

Labome :

INI1-antibody

SMARCB1 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

SMARCB1 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)