Gene:
Gene Symbol:
VPS4
Gene Name:
AAA family ATPase VPS4
Original Clone ID:
WISP12-168
Keyword:
oligomerization disrupted
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pRS416
Format:
CLOSED
Source:
University of Utah
Description:
None
Comments:
pRS416+Vps4p(L151D)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Mary Anne Karren
Wesley I. Sundquist
Wesley I. Sundquist
Mary Anne Karren
ScCD00597456
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 1314nts
Open reading frame: 1 to 1314
ATGAGCACGGGAGATTTTTTAACTAAGGGAATAGAACTGGTTCAAAAGGCCATTGACCTG
GACACAGCCACGCAGTATGAGGAGGCGTATACAGCATACTATAACGGATTAGATTATTTG
ATGTTGGCTTTGAAATACGAGAAAAACCCTAAGTCGAAGGATTTGATAAGAGCGAAGTTT
ACAGAGTATCTAAACCGTGCAGAGCAGTTGAAAAAGCACTTGGAAAGTGAAGAGGCAAAC
GCGGCAAAGAAATCTCCTAGCGCTGGTAGTGGTTCCAACGGTGGTAATAAGAAAATCTCT
CAGGAAGAGGGTGAGGATAATGGTGGAGAAGACAACAAAAAGCTAAGAGGTGCCTTATCG
AGTGCTATTTTGTCTGAGAAGCCCAATGTTAAGTGGGAAGACGTGGCTGGACTGGAAGGA
GCCAAAGAGGCCCTTAAAGAAGCCGTTATTGATCCTGTCAAGTTCCCACATTTGTTCAAA
GGTAACCGTAAGCCCACTTCGGGAATCTTATTGTACGGACCACCAGGTACAGGTAAATCA
TATTTGGCAAAAGCAGTGGCCACCGAGGCCAATTCTACCTTTTTCAGTGTTAGTTCCAGT
GATTTGGTTTCTAAGTGGATGGGTGAATCTGAAAAACTTGTCAAACAGTTATTTGCGATG
GCAAGAGAAAATAAACCTTCTATTATTTTTATAGATGAAGTGGATGCGCTAACAGGTACC
AGAGGAGAAGGAGAGAGCGAAGCAAGTAGAAGAATTAAAACAGAATTATTGGTACAAATG
AATGGTGTTGGGAATGACTCCCAAGGTGTTTTGGTCCTTGGCGCAACAAATATCCCATGG
CAACTGGACAGTGCCATCAGGAGAAGATTTGAAAGAAGAATATATATACCATTACCAGAT
TTGGCGGCAAGAACCACCATGTTCGAGATTAACGTCGGCGACACTCCATGCGTATTGACC
AAGGAAGATTATAGAACTTTAGGTGCAATGACTGAAGGTTATTCCGGGAGTGATATTGCT
GTAGTGGTGAAAGATGCGCTAATGCAACCGATAAGAAAAATTCAAAGCGCGACACATTTC
AAAGATGTCTCTACGGAGGACGACGAAACAAGAAAACTAACACCATGCTCTCCAGGTGAT
GATGGCGCCATCGAGATGAGTTGGACGGATATTGAAGCTGATGAATTAAAAGAACCAGAT
CTCACCATAAAGGATTTCTTAAAGGCTATCAAATCGACGAGACCTACCGTGAATGAGGAT
GACTTGCTGAAGCAAGAACAGTTCACTAGAGATTTTGGTCAAGAAGGTAACTAA
Protein sequence predicted based on nucleotide sequence
MSTGDFLTKGIELVQKAIDLDTATQYEEAYTAYYNGLDYLMLALKYEKNPKSKDLIRAKF TEYLNRAEQLKKHLESEEANAAKKSPSAGSGSNGGNKKISQEEGEDNGGEDNKKLRGALS SAILSEKPNVKWEDVAGLEGAKEALKEAVIDPVKFPHLFKGNRKPTSGILLYGPPGTGKS YLAKAVATEANSTFFSVSSSDLVSKWMGESEKLVKQLFAMARENKPSIIFIDEVDALTGT RGEGESEASRRIKTELLVQMNGVGNDSQGVLVLGATNIPWQLDSAIRRRFERRIYIPLPD LAARTTMFEINVGDTPCVLTKEDYRTLGAMTEGYSGSDIAVVVKDALMQPIRKIQSATHF KDVSTEDDETRKLTPCSPGDDGAIEMSWTDIEADELKEPDLTIKDFLKAIKSTRPTVNED DLLKQEQFTRDFGQEGN*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
L151D
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
URA
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Expressed
Recommended expression in:
Not Applicable
Vector Name:
pRS416
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Yeast centromere shuttle plasmid with a T7 promoter; ampicillin resistance in bacteria, URA3 resistance in yeast; restriction enzyme cloning
Comments:
Restriction enzymes BamHI, Sal1 and be used to clone into this vector
Size (bp):
4898
Parent Vector:
None
Properties:
multiple cloning site, yeast shuttle
Author Name:
Stratagene
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
f1
f1 origin
1483
1798
bacterial origin
ColE
ColE1 bacterial origin
2547
3229
bacterial origin
M13 Ori
M13 origin
1351
1806
primer
M13 forward
M13 forward sequencing primer
1951
1966
primer
M13 reverse
M13 reverse sequencing primer
2162
2179
promoter
T7
T7 promoter
1976
1996
promoter
Ura3 promoter
Ura3 promoter
302
331
selectable marker
AmpR
ampicillin resistance gene
3327
3986
selectable marker
Ura3
Ura3
417
1220
trxn regulatory element
lac operator
lac operator
2185
2207
yeast centromere
CEN6
CEN6-ARS4 yeast centromere
4316
4834
