Gene:
Gene Symbol:
CHMP4B
Gene Name:
charged multivesicular body protein 4B
Original Clone ID:
WISP03-212
Keyword:
Full length CHMP4B (1-224)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGBKT7
Format:
CLOSED
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI:Biology
HsCD00597686
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 675nts
Open reading frame: 51 to 725
GAGCCGCCATCATGGAGGAGCAGAAGCTGATCTCAGAGGAGGACCTGCATATGTCGGTGT
TCGGCAAGCTGTTCGGGGCTGGAGGGGGTAAGGCCGGCAAGGGCGGCCCGACCCCCCAGG
AGGCCATCCAGCGGCTGCGGGACACGGAAGAGATGTTAAGCAAGAAACAGGAGTTCCTGG
AGAAGAAAATCGAGCAGGAGCTGACGGCCGCCAAGAAGCACGGCACCAAAAACAAGCGCG
CGGCCCTCCAGGCACTGAAGCGTAAGAAGAGGTATGAGAAGCAGCTGGCGCAGATCGACG
GCACATTATCAACCATCGAGTTCCAGCGGGAGGCCCTGGAGAATGCCAACACCAACACCG
AGGTGCTCAAGAACATGGGCTATGCCGCCAAGGCCATGAAGGCGGCCCATGACAACATGG
ACATCGATAAAGTTGATGAGTTAATGCAGGACATTGCTGACCAGCAAGAACTTGCAGAGG
AGATTTCAACAGCAATTTCGAAACCTGTAGGGTTTGGAGAAGAGTTTGACGAGGATGAGC
TCATGGCGGAATTAGAAGAACTAGAACAGGAGGAACTAGACAAGAATTTGCTGGAAATCA
GTGGACCCGAAACAGTCCCTCTACCAAATGTTCCCTCTATAGCCCTACCATCAAAACCCG
CCAAGAAGAAAGAAGAGGAGGACGACGACATGAAGGAATTGGAGAACTGGGCTGGCTCTA
TGTAAGGATCCGTCGACCTGCAGCGGCCGCATAACTAGCATAACCCCTTGGGGCCT
Protein sequence predicted based on nucleotide sequence
MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTK NKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAH DNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNL LEISGPETVPLPNVPSIALPSKPAKKKEEEDDDMKELENWAGSM*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g15c,G5G;a666c,G222G;
c669t,S223S
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
TRP
Host Type: bacterial Marker: kanamycin
Host Type: bacterial Marker: kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Expressed
Recommended expression in:
Not Applicable
Vector Name:
pGBKT7
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Yeast two-hybrid vector with a T7 and ADH1 promoter and N-terminal c-myc tag; TRP1 resistance in yeast, kanamycin resistance in bacteria; restriction enzyme cloning
Comments:
pGADT7 AD is a yeast two-hybrid expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest.
Size (bp):
7303
Parent Vector:
None
Properties:
multiple cloning site, two-hybrid, with tag/fusion/marker, yeast expression
Author Name:
Clonetech
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
Multiple cloning site
1281
1334
bacterial origin
pUC ori
pUC origin
1838
2636
binding site
Gal4 Act
Gal4 DNA binding domain
762
1202
promoter
ADH1
truncated ADH1 promoter
30
736
promoter
T7
T7 promoter
1212
1235
selectable marker
KanR
Kanamycin resistance
3221
4143
selectable marker
TRP1
TRP1 selectable marker in yeast
6030
6704
tag
c-myc
N-terminal c-myc tag
1248
1280
trxn termination sequence
T7 term
T7 terminator
1335
1381
trxn termination sequence
ADH1 term
ADH1 transcriptional terminator
1414
1610
