DNASU Plasmid | RFC3 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RFC3

Gene Name: replication factor C (activator 1) 3, 38kDa

Original Clone ID:

HR2902C-1-271-15.7

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00598043

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 816nts

Open reading frame: 34 to 849

Reference Sequence Alignment

ACCATGGGCCATCACCATCACCATCACAGCCATATGAGCCTCTGGGTGGACAAGTATCGG
CCCTGCTCCTTGGGACGGCTGGACTATCACAAGGAGCAGGCGGCCCAGCTGCGGAACCTG
GTGCAGTGTGGTGACTTTCCTCATCTGTTAGTGTACGGACCATCAGGTGCTGGAAAAAAG
ACAAGAATTATGTGTATTCTACGTGAACTTTATGGTGTTGGAGTGGAAAAATTGAGAATT
GAACATCAGACCATCACAACTCCATCTAAAAAAAAAATTGAAATTAGCACCATTGCAAGT
AACTACCACCTTGAAGTTAATCCTAGTGATGCTGGAAATAGTGACCGAGTAGTCATTCAG
GAGATGTTGAAAACAGTGGCACAATCACAACAACTTGAAACAAACTCTCAAAGGGATTTT
AAAGTGGTATTATTGACAGAAGTTGACAAACTCACCAAAGATGCTCAGCATGCCTTGCGA
AGAACCATGGAAAAATATATGTCTACCTGCAGATTGATCTTGTGCTGCAATTCTACATCT
AAAGTGATCCCACCTATTCGTAGTAGGTGCTTGGCGGTTCGTGTGCCTGCTCCCAGCATT
GAAGATATTTGCCACGTGTTATCTACTGTGTGTAAGAAGGAAGGTCTGAATCTTCCTTCA
CAACTGGCTCATAGACTTGCAGAGAAGTCTTGTAGAAATCTCAGAAAAGCCCTGCTTATG
TGTGAAGCCTGCAGAGTGCAACAATATCCTTTTACTGCAGATCAAGAAATCCCTGAGACA
GATTGGGAGGTGTATCTGAGGGAGACTGCAAATGCTATTGTCAGTCAGCAAACTCCACAA
AGGCTCTAG

Protein sequence predicted based on nucleotide sequence

MSLWVDKYRPCSLGRLDYHKEQAAQLRNLVQCGDFPHLLVYGPSGAGKKTRIMCILRELY
GVGVEKLRIEHQTITTPSKKKIEISTIASNYHLEVNPSDAGNSDRVVIQEMLKTVAQSQQ
LETNSQRDFKVVLLTEVDKLTKDAQHALRRTMEKYMSTCRLILCCNSTSKVIPPIRSRCL
AVRVPAPSIEDICHVLSTVCKKEGLNLPSQLAHRLAEKSCRNLRKALLMCEACRVQQYPF
TADQEIPETDWEVYLRETANAIVSQQTPQRL*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t166c,L56L

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR2902C

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

RFC3

Symbol Nomenclature :

RFC3

SYNONYM :

RFC38

Designation :

Full Nomenclature :

replication factor C (activator 1) 3, 38kDa

GENEID :

5983

GI :

108773789

GenBank Accession :

NM_181558

HGNC :

9971

MIM :

600405

Vega :

OTTHUMG00000016715

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00598043      ACCATGGGCCATCACCATCACCATCACAGCCATATGAGCCTCTGGGTGGACAAGTATCGG
NM_002915.3       ---------------------------------ATGAGCCTCTGGGTGGACAAGTATCGG
NM_181558.2       ---------------------------------ATGAGCCTCTGGGTGGACAAGTATCGG
                                                   ***************************

HsCD00598043      CCCTGCTCCTTGGGACGGCTGGACTATCACAAGGAGCAGGCGGCCCAGCTGCGGAACCTG
NM_002915.3       CCCTGCTCCTTGGGACGGCTGGACTATCACAAGGAGCAGGCGGCCCAGCTGCGGAACCTG
NM_181558.2       CCCTGCTCCTTGGGACGGCTGGACTATCACAAGGAGCAGGCGGCCCAGCTGCGGAACCTG
                  ************************************************************

HsCD00598043      GTGCAGTGTGGTGACTTTCCTCATCTGTTAGTGTACGGACCATCAGGTGCTGGAAAAAAG
NM_002915.3       GTGCAGTGTGGTGACTTTCCTCATCTGTTAGTGTACGGACCATCAGGTGCTGGAAAAAAG
NM_181558.2       GTGCAGTGTGGTGACTTTCCTCATCTGTTAGTGTACGGACCATCAGGTGCTGGAAAAAAG
                  ************************************************************

HsCD00598043      ACAAGAATTATGTGTATTCTACGTGAACTTTATGGTGTTGGAGTGGAAAAATTGAGAATT
NM_002915.3       ACAAGAATTATGTGTATTCTACGTGAACTTTATGGTGTTGGAGTGGAAAAATTGAGAATT
NM_181558.2       ACAAGAATTATGTGTATTCTACGTGAACTTTATGGTGTTGGAGTGGAAAAATTGAGAATT
                  ************************************************************

HsCD00598043      GAACATCAGACCATCACAACTCCATCTAAAAAAAAAATTGAAATTAGCACCATTGCAAGT
NM_002915.3       GAACATCAGACCATCACAACTCCATCTAAAAAAAAAATTGAAATTAGCACCATTGCAAGT
NM_181558.2       GAACATCAGACCATCACAACTCCATCTAAAAAAAAAATTGAAATTAGCACCATTGCAAGT
                  ************************************************************

HsCD00598043      AACTACCACCTTGAAGTTAATCCTAGTGATGCTGGAAATAGTGACCGAGTAGTCATTCAG
NM_002915.3       AACTACCACCTTGAAGTTAATCCTAGTGATGCTGGAAATAGTGACCGAGTAGTCATTCAG
NM_181558.2       AACTACCACCTTGAAGTTAATCCTAGTGATGCTGGAAATAGTGACCGAGTAGTCATTCAG
                  ************************************************************

HsCD00598043      GAGATGTTGAAAACAGTGGCACAATCACAACAACTTGAAACAAACTCTCAAAGGGATTTT
NM_002915.3       GAGATGTTGAAAACAGTGGCACAATCACAACAACTTGAAACAAACTCTCAAAGGGATTTT
NM_181558.2       GAGATGTTGAAAACAGTGGCACAATCACAACAACTTGAAACAAACTCTCAAAGGGATTTT
                  ************************************************************

HsCD00598043      AAAGTGGTATTATTGACAGAAGTTGACAAACTCACCAAAGATGCTCAGCATGCCTTGCGA
NM_002915.3       AAAGTGGTATTATTGACAGAAGTTGACAAACTCACCAAAGATGCTCAGCATGCCTTGCGA
NM_181558.2       AAAGTGGTATTATTGACAGAAGTTGACAAACTCACCAAAGATGCTCAGCATGCCTTGCGA
                  ************************************************************

HsCD00598043      AGAACCATGGAAAAATATATGTCTACCTGCAGATTGATCTTGTGCTGCAATTCTACATCT
NM_002915.3       AGAACCATGGAAAAATATATGTCTACCTGCAGATTGATCTTGTGCTGCAATTCTACATCT
NM_181558.2       AGAACCATGGAAAAATATATGTCTACCTGCAGATTGATCTTGTGCTGCAATTCTACATCT
                  ************************************************************

HsCD00598043      AAAGTGATCCCACCTATTCGTAGTAGGTGCTTGGCGGTTCGTGTGCCTGCTCCCAGCATT
NM_002915.3       AAAGTGATCCCACCTATTCGTAGTAGGTGCTTGGCGGTTCGTGTGCCTGCTCCCAGCATT
NM_181558.2       AAAGTGATCCCACCTATTCGTAGTAGGTGCTTGGCGGTTCGTGTGCCTGCTCCCAGCATT
                  ************************************************************

HsCD00598043      GAAGATATTTGCCACGTGTTATCTACTGTGTGTAAGAAGGAAGGTCTGAATCTTCCTTCA
NM_002915.3       GAAGATATTTGCCACGTGTTATCTACTGTGTGTAAGAAGGAAGGTCTGAATCTTCCTTCA
NM_181558.2       GAAGATATTTGCCACGTGTTATCTACTGTGTGTAAGAAGGAAGGTCTGAATCTTCCTTCA
                  ************************************************************

HsCD00598043      CAACTGGCTCATAGACTTGCAGAGAAGTCTTGTAGAAATCTCAGAAAAGCCCTGCTTATG
NM_002915.3       CAACTGGCTCATAGACTTGCAGAGAAGTCTTGTAGAAATCTCAGAAAAGCCCTGCTTATG
NM_181558.2       CAACTGGCTCATAGACTTGCAGAGAAGTCTTGTAGAAATCTCAGAAAAGCCCTGCTTATG
                  ************************************************************

HsCD00598043      TGTGAAGCCTGCAGAGTGCAACAATATCCTTTTACTGCAGATCAAGAAATCCCTGAGACA
NM_002915.3       TGTGAAGCCTGCAGAGTGCAACAATATCCTTTTACTGCAGATCAAGAAATCCCTGAGACA
NM_181558.2       TGTGAAGCCTGCAGAGTGCAACAATATCCTTTTACTGCAGATCAAGAAATCCCTGAGACA
                  ************************************************************

HsCD00598043      GATTGGGAGGTGTATCTGAGGGAGACTGCAAATGCTATTGTCAGTCAGCAAACTCCACAA
NM_002915.3       GATTGGGAGGTGTATCTGAGGGAGACTGCAAATGCTATTGTCAGTCAGCAAACTCCACAA
NM_181558.2       GATTGGGAGGTGTATCTGAGGGAGACTGCAAATGCTATTGTCAGTCAGCAAACTCCACAA
                  ************************************************************

HsCD00598043      AGGCTCTAG---------------------------------------------------
NM_002915.3       AGGCTCCTTGAAGTTCGTGGAAGGCTGTATGAGCTTCTAACTCATTGTATTCCTCCTGAG
NM_181558.2       AGGCTCCTTGAAGTTCGTGGAAGGCTGTATGAGCTTCTAACTCATTGTATTCCTCCTGAG
                  ****** :                                                    

HsCD00598043      ------------------------------------------------------------
NM_002915.3       ATAATAATGAAGGGCCTTCTCTCAGAACTGTTACATAATTGTGATGGACAACTGAAAGGG
NM_181558.2       ATAATAATGAAGGCATGTAAGGAGGAATCAAGAAGCTGTGACATATTCTGA---------
                                                                              

HsCD00598043      ------------------------------------------------------------
NM_002915.3       GAGGTGGCACAAATGGCAGCTTACTATGAGCATCGTCTACAGCTGGGTAGCAAAGCCATT
NM_181558.2       ------------------------------------------------------------
                                                                              

HsCD00598043      ------------------------------------------------------------
NM_002915.3       TATCACTTGGAAGCGTTTGTGGCCAAATTCATGGCACTTTATAAGAAGTTCATGGAGGAT
NM_181558.2       ------------------------------------------------------------
                                                                              

HsCD00598043      ------------------------
NM_002915.3       GGATTGGAAGGCATGATGTTCTGA
NM_181558.2       ------------------------

Pathways

NCI :

ATR signaling pathway

NCI :

Fanconi anemia pathway

Panther :

DNA replication

Reactome :

Activation of ATR in response to replication stress

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Chromosome Maintenance

Reactome :

DNA Repair

Reactome :

DNA Replication

Reactome :

DNA strand elongation

Reactome :

Extension of Telomeres

Reactome :

G2/M Checkpoints

Reactome :

Gap-filling DNA repair synthesis and ligation in GG-NER

Reactome :

Gap-filling DNA repair synthesis and ligation in TC-NER

Reactome :

Global Genomic NER (GG-NER)

Reactome :

Lagging Strand Synthesis

Reactome :

Leading Strand Synthesis

Reactome :

Nucleotide Excision Repair

Reactome :

Polymerase switching

Reactome :

Polymerase switching on the C-strand of the telomere

Reactome :

Repair synthesis for gap-filling by DNA polymerase in TC-NER

Reactome :

Repair synthesis of patch ~27-30 bases long by DNA polymerase

Reactome :

S Phase

Reactome :

Synthesis of DNA

Reactome :

Telomere C-strand (Lagging Strand) Synthesis

Reactome :

Telomere Maintenance

Reactome :

Transcription-coupled NER (TC-NER)

WikiPathway :

DNA Replication

Protein

Structural Annotation Wiki :

HR2902C

UniProt :

P40938

HPRD :

02676

Clone

Cloning Information :

626124

Original Clone ID :

HR2902C-1-271-15.7

Experimental

TargetTrack - Experimental data :

HR2902C

Organism

Species Specific ID:

5983

Genome

Chromosome :

Map Location :

13q13.2

Ensembl :

ENSG00000133119

Reagents

Labome :

RFC3-antibody

RFC3 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

RFC3 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)