DNASU Plasmid | DHX9 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: DHX9

Gene Name: DEAH (Asp-Glu-Ala-His) box helicase 9

Original Clone ID:

HR6324C-824-1150-15.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

FUSION

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00598156

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 984nts

Open reading frame: 51 to 1034

Reference Sequence Alignment

TTTAAGAAGGAGATATACCATGGGGCCATCACCATCACCATCACAGCCATATGCCCCCTT
TGGATGCTGTGATGGTAGCAGAACACACTCTTAGAGAGCTTGATGCATTAGATGCCAATG
ATGAGTTGACTCCTTTGGGACGAATCCTGGCTAAACTCCCCATTGAGCCTCGTTTTGGCA
AAATGATGATAATGGGGTGTATTTTCTACGTGGGAGATGCTATCTGTACCATTGCTGCTG
CTACCTGCTTTCCAGAGCCTTTCATCAATGAAGGAAAGCGGCTGGGCTATATCCATCGAA
ATTTTGCTGGAAACAGATTTTCTGATCACGTAGCCCTTTTATCAGTATTCCAAGCCTGGG
ATGATGCTAGAATGGGTGGAGAAGAAGCAGAGATACGTTTTTGTGAGCACAAAAGACTTA
ATATGGCTACACTAAGAATGACCTGGGAAGCCAAAGTTCAGCTCAAAGAGATTTTGATTA
ATTCTGGGTTTCCAGAAGATTGTTTGTTGACACAAGTGTTTACTAACACTGGACCAGATA
ATAATTTGGATGTTGTTATCTCCCTCCTGGCCTTTGGTGTGTACCCCAATGTATGCTATC
ATAAGGAAAAGAGGAAGATTCTCACCACTGAAGGGCGTAATGCACTTATCCACAAATCAT
CTGTTAATTGTCCTTTTAGTAGCCAAGACATGAAGTACCCATCTCCCTTCTTTGTATTTG
GTGAAAAGATTCGAACTCGAGCCATCTCTGCTAAAGGCATGACTTTAGTCACCCCCCTGC
AGTTGCTTCTCTTTGCCTCCAAGAAAGTCCAATCTGATGGGCAGATTGTGCTTGTAGATG
ACTGGATTAAACTGCAAATATCTCATGAAGCTGCTGCCTGTATCACTGGTCTCCGGGCAG
CCATGGAGGCTTTGGTTGTTGAAGTAACCAAACAACCTGCTATCATCAGCCAGTTGGACC
CCGTAAATGAACGTATGCTGAACATGATCCGTCAGATCTCTAGACCCTCAGCTGCTGGTA
TCAACCTTATGATTAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAAG

Protein sequence predicted based on nucleotide sequence

MPPLDAVMVAEHTLRELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAICT
IAAATCFPEPFINEGKRLGYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEAEIRFCEH
KRLNMATLRMTWEAKVQLKEILINSGFPEDCLLTQVFTNTGPDNNLDVVISLLAFGVYPN
VCYHKEKRKILTTEGRNALIHKSSVNCPFSSQDMKYPSPFFVFGEKIRTRAISAKGMTLV
TPLQLLLFASKKVQSDGQIVLVDDWIKLQISHEAAACITGLRAAMEALVVEVTKQPAIIS
QLDPVNERMLNMIRQISRPSAAGINLMI

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t24g,I8M;a26t,E9V;t393c,T131T

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR6324C

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

DHX9

Symbol Nomenclature :

DHX9

SYNONYM :

DDX9; LKP; NDH2; NDHII; RHA

Designation :

Full Nomenclature :

DEAH (Asp-Glu-Ala-His) box helicase 9

GENEID :

1660

GenBank Accession :

NM_030588

HGNC :

2750

MIM :

603115

Vega :

OTTHUMG00000035337

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00598156      ------------------------------------------------------------
NM_001357.4       ATGGGTGACGTTAAAAATTTTCTGTATGCCTGGTGTGGCAAAAGGAAGATGACCCCATCC
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       TATGAAATTAGAGCAGTGGGGAACAAAAACAGGCAGAAATTCATGTGTGAGGTTCAGGTG
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GAAGGTTATAATTACACTGGCATGGGAAATTCCACCAATAAAAAAGATGCACAAAGCAAT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GCTGCCAGAGACTTTGTTAACTATTTGGTTCGAATAAATGAAATAAAGAGTGAAGAAGTT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CCAGCTTTTGGGGTAGCATCTCCGCCCCCACTTACTGATACTCCTGACACTACAGCAAAT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GCTGAAGGAGATTTACCAACAACCATGGGAGGACCTCTTCCTCCACATCTGGCTCTCAAA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GCAGAAAATAATTCTGAGGTAGGGGCCTCTGGCTATGGTGTTCCTGGGCCCACCTGGGAC
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CGAGGAGCCAACTTGAAGGATTACTACTCAAGAAAGGAAGAACAAGAAGTGCAAGCGACT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CTAGAATCAGAAGAAGTGGATTTAAATGCTGGGCTTCATGGAAACTGGACCTTGGAAAAT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GCTAAAGCTCGTCTAAACCAATATTTTCAGAAAGAAAAGATCCAAGGAGAATATAAGTAC
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       ACCCAAGTGGGTCCTGATCACAACAGGAGCTTTATTGCAGAAATGACCATTTATATCAAG
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CAGCTGGGCAGAAGGATTTTTGCACGAGAACATGGATCAAATAAGAAATTGGCAGCACAG
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       TCCTGTGCCCTGTCACTTGTCAGACAACTGTACCATCTTGGAGTGGTTGAAGCTTACTCC
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GGACTTACAAAGAAGAAGGAAGGAGAGACAGTGGAGCCTTACAAAGTAAACCTCTCTCAA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GATTTAGAGCATCAGCTGCAAAACATCATTCAAGAGCTAAATCTTGAGATTTTGCCCCCG
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CCTGAAGATCCTTCTGTGCCAGTTGCACTCAACATTGGCAAATTGGCTCAGTTCGAACCA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       TCTCAGCGACAAAACCAAGTGGGTGTGGTTCCTTGGTCACCTCCACAATCCAACTGGAAT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CCTTGGACTAGTAGCAACATTGATGAGGGGCCTCTGGCTTTTGCTACTCCAGAGCAAATA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       AGCATGGACCTCAAGAATGAATTGATGTACCAGTTGGAACAGGATCATGATTTGCAAGCA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       ATCTTGCAGGAGAGAGAGTTACTGCCTGTGAAGAAATTTGAAAGTGAGATTCTGGAAGCA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       ATCAGCCAAAATTCAGTTGTCATTATTAGAGGGGCTACTGGATGTGGGAAAACCACACAG
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GTTCCCCAGTTCATTCTAGATGACTTTATCCAGAATGACCGAGCAGCAGAGTGTAACATC
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GTAGTAACTCAGCCCAGAAGAATCAGTGCGGTTTCTGTGGCAGAGCGAGTTGCATTTGAA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       AGAGGAGAAGAGCCTGGAAAAAGCTGTGGCTACAGCGTTCGATTTGAGTCTATACTTCCT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CGTCCTCATGCCAGTATAATGTTTTGTACTGTAGGTGTGCTCCTGAGAAAATTAGAAGCA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GGCATTCGAGGAATCAGTCATGTAATTGTAGATGAAATACATGAAAGAGATATTAATACT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GACTTCCTTTTGGTAGTACTGCGTGATGTTGTTCAGGCTTATCCTGAAGTTCGCATTGTT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CTTATGTCTGCTACTATTGATACCAGCATGTTTTGTGAATATTTCTTCAATTGCCCCATC
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       ATTGAAGTTTATGGGAGGACTTACCCAGTTCAAGAATATTTTCTGGAAGACTGCATTCAG
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       ATGACCCACTTTGTTCCTCCACCAAAAGACAAAAAGAAGAAGGATAAGGATGATGATGGT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GGTGAGGATGATGATGCAAATTGCAACTTGATCTGTGGTGATGAATATGGTCCAGAAACA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       AGGTTGAGCATGTCTCAATTGAACGAAAAGGAAACTCCTTTTGAACTCATCGAGGCTCTA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CTTAAGTACATTGAAACCCTTAATGTTCCTGGAGCTGTGTTGGTTTTTTTGCCTGGCTGG
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       AATCTGATTTATACTATGCAGAAGCATTTGGAAATGAATCCACATTTTGGAAGCCATCGG
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       TATCAGATTCTACCCCTGCATTCTCAGATTCCTCGAGAGGAACAGCGCAAAGTGTTTGAT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CCAGTACCAGTTGGAGTAACCAAGGTTATTTTGTCCACAAATATTGCTGAAACAAGCATT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       ACCATAAACGATGTTGTTTATGTCATTGACTCCTGCAAGCAGAAAGTGAAACTCTTCACT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GCTCACAACAATATGACCAACTATGCTACCGTATGGGCATCAAAAACAAACCTTGAGCAA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CGGAAAGGGCGAGCTGGCCGAGTACGGCCTGGATTCTGCTTTCACCTGTGCAGCCGAGCT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       CGTTTTGAGAGACTTGAAACCCACATGACACCAGAGATGTTCCGAACACCATTGCATGAA
                                                                              

HsCD00598156      ----------------TTTAAGAAGGAGATATACCATGGGGCCATCACCATCACCATCAC
NM_001357.4       ATTGCTCTTAGCATAAAACTTCTGCGTCTAGGAGGAATTGGCCAATTTCTGGCCAAAGCA
                                  :: :: :. *: ::. *  *:  *****: : *:  .*.*: ..

HsCD00598156      AGCCATATGCCCCCTTTGGATGCTGTGATGGTAGCAGAACACACTCTTAGAGAGCTTGAT
NM_001357.4       ATTGAACCTCCCCCTTTGGATGCTGTGATTGAAGCAGAACACACTCTTAGAGAGCTTGAT
                  *   *:.  ******************** *:****************************

HsCD00598156      GCATTAGATGCCAATGATGAGTTGACTCCTTTGGGACGAATCCTGGCTAAACTCCCCATT
NM_001357.4       GCATTAGATGCCAATGATGAGTTGACTCCTTTGGGACGAATCCTGGCTAAACTCCCCATT
                  ************************************************************

HsCD00598156      GAGCCTCGTTTTGGCAAAATGATGATAATGGGGTGTATTTTCTACGTGGGAGATGCTATC
NM_001357.4       GAGCCTCGTTTTGGCAAAATGATGATAATGGGGTGTATTTTCTACGTGGGAGATGCTATC
                  ************************************************************

HsCD00598156      TGTACCATTGCTGCTGCTACCTGCTTTCCAGAGCCTTTCATCAATGAAGGAAAGCGGCTG
NM_001357.4       TGTACCATTGCTGCTGCTACCTGCTTTCCAGAGCCTTTCATCAATGAAGGAAAGCGGCTG
                  ************************************************************

HsCD00598156      GGCTATATCCATCGAAATTTTGCTGGAAACAGATTTTCTGATCACGTAGCCCTTTTATCA
NM_001357.4       GGCTATATCCATCGAAATTTTGCTGGAAACAGATTTTCTGATCACGTAGCCCTTTTATCA
                  ************************************************************

HsCD00598156      GTATTCCAAGCCTGGGATGATGCTAGAATGGGTGGAGAAGAAGCAGAGATACGTTTTTGT
NM_001357.4       GTATTCCAAGCCTGGGATGATGCTAGAATGGGTGGAGAAGAAGCAGAGATACGTTTTTGT
                  ************************************************************

HsCD00598156      GAGCACAAAAGACTTAATATGGCTACACTAAGAATGACCTGGGAAGCCAAAGTTCAGCTC
NM_001357.4       GAGCACAAAAGACTTAATATGGCTACACTAAGAATGACCTGGGAAGCCAAAGTTCAGCTC
                  ************************************************************

HsCD00598156      AAAGAGATTTTGATTAATTCTGGGTTTCCAGAAGATTGTTTGTTGACACAAGTGTTTACT
NM_001357.4       AAAGAGATTTTGATTAATTCTGGGTTTCCAGAAGATTGTTTGTTGACACAAGTGTTTACT
                  ************************************************************

HsCD00598156      AACACTGGACCAGATAATAATTTGGATGTTGTTATCTCCCTCCTGGCCTTTGGTGTGTAC
NM_001357.4       AACACTGGACCAGATAATAATTTGGATGTTGTTATCTCCCTCCTGGCCTTTGGTGTGTAC
                  ************************************************************

HsCD00598156      CCCAATGTATGCTATCATAAGGAAAAGAGGAAGATTCTCACCACTGAAGGGCGTAATGCA
NM_001357.4       CCCAATGTATGCTATCATAAGGAAAAGAGGAAGATTCTCACCACTGAAGGGCGTAATGCA
                  ************************************************************

HsCD00598156      CTTATCCACAAATCATCTGTTAATTGTCCTTTTAGTAGCCAAGACATGAAGTACCCATCT
NM_001357.4       CTTATCCACAAATCATCTGTTAATTGTCCTTTTAGTAGCCAAGACATGAAGTACCCATCT
                  ************************************************************

HsCD00598156      CCCTTCTTTGTATTTGGTGAAAAGATTCGAACTCGAGCCATCTCTGCTAAAGGCATGACT
NM_001357.4       CCCTTCTTTGTATTTGGTGAAAAGATTCGAACTCGAGCCATCTCTGCTAAAGGCATGACT
                  ************************************************************

HsCD00598156      TTAGTCACCCCCCTGCAGTTGCTTCTCTTTGCCTCCAAGAAAGTCCAATCTGATGGGCAG
NM_001357.4       TTAGTCACCCCCCTGCAGTTGCTTCTCTTTGCCTCCAAGAAAGTCCAATCTGATGGGCAG
                  ************************************************************

HsCD00598156      ATTGTGCTTGTAGATGACTGGATTAAACTGCAAATATCTCATGAAGCTGCTGCCTGTATC
NM_001357.4       ATTGTGCTTGTAGATGACTGGATTAAACTGCAAATATCTCATGAAGCTGCTGCCTGTATC
                  ************************************************************

HsCD00598156      ACTGGTCTCCGGGCAGCCATGGAGGCTTTGGTTGTTGAAGTAACCAAACAACCTGCTATC
NM_001357.4       ACTGGTCTCCGGGCAGCCATGGAGGCTTTGGTTGTTGAAGTAACCAAACAACCTGCTATC
                  ************************************************************

HsCD00598156      ATCAGCCAGTTGGACCCCGTAAATGAACGTATGCTGAACATGATCCGTCAGATCTCTAGA
NM_001357.4       ATCAGCCAGTTGGACCCCGTAAATGAACGTATGCTGAACATGATCCGTCAGATCTCTAGA
                  ************************************************************

HsCD00598156      CCCTCAGCTGCTGGTATCAACCTTATGATTAGCTCGAGATCCGGCTGCTAACAAAGCCCG
NM_001357.4       CCCTCAGCTGCTGGTATCAACCTTATGATTGGCAGTACACGGTATGGAGATGGTCCACGT
                  ******************************.**:  * *    .  *. *: .:. .*  

HsCD00598156      AAAG--------------------------------------------------------
NM_001357.4       CCTCCCAAGATGGCCCGATACGACAATGGAAGCGGATATAGAAGGGGAGGTTCTAGTTAC
                  ..:                                                         

HsCD00598156      ------------------------------------------------------------
NM_001357.4       AGTGGTGGAGGCTATGGCGGTGGCTATAGCAGTGGAGGCTATGGTAGCGGAGGCTATGGT
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GGCAGCGCCAACTCCTTTCGGGCAGGATATGGTGCAGGTGTTGGTGGAGGCTATAGAGGA
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       GTTTCCCGAGGTGGCTTTAGAGGCAACTCTGGAGGAGACTACAGAGGGCCTAGTGGAGGC
                                                                              

HsCD00598156      ------------------------------------------------------------
NM_001357.4       TACAGAGGATCTGGGGGATTCCAGCGAGGAGGTGGTAGGGGGGCCTATGGAACTGGCTAC
                                                                              

HsCD00598156      ---------------------------------
NM_001357.4       TTTGGACAGGGAAGAGGAGGTGGCGGCTATTAA

Pathways

Reactome :

Activated TLR4 signalling

Reactome :

Cytosolic sensors of pathogen-associated DNA

Reactome :

DEx/H-box helicases activate type I IFN and inflammatory cytokines production

Reactome :

Gene Expression

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

mRNA Splicing

Reactome :

mRNA Splicing - Major Pathway

Reactome :

MyD88 cascade initiated on plasma membrane

Reactome :

MyD88 dependent cascade initiated on endosome

Reactome :

MyD88-independent cascade

Reactome :

MyD88:Mal cascade initiated on plasma membrane

Reactome :

Processing of Capped Intron-Containing Pre-mRNA

Reactome :

RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways

Reactome :

RIP-mediated NFkB activation via ZBP1

Reactome :

TAK1 activates NFkB by phosphorylation and activation of IKKs complex

Reactome :

Toll Like Receptor 10 (TLR10) Cascade

Reactome :

Toll Like Receptor 2 (TLR2) Cascade

Reactome :

Toll Like Receptor 3 (TLR3) Cascade

Reactome :

Toll Like Receptor 4 (TLR4) Cascade

Reactome :

Toll Like Receptor 5 (TLR5) Cascade

Reactome :

Toll Like Receptor 7/8 (TLR7/8) Cascade

Reactome :

Toll Like Receptor 9 (TLR9) Cascade

Reactome :

Toll Like Receptor TLR1:TLR2 Cascade

Reactome :

Toll Like Receptor TLR6:TLR2 Cascade

Reactome :

Toll-Like Receptors Cascades

Reactome :

TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation

Reactome :

TRAF6 Mediated Induction of proinflammatory cytokines

Reactome :

TRAF6 mediated NF-kB activation

Reactome :

TRIF-mediated TLR3/TLR4 signaling

Reactome :

ZBP1(DAI) mediated induction of type I IFNs

WikiPathway :

mRNA processing

Protein

Structural Annotation Wiki :

HR6324C

UniProt :

Q08211

HPRD :

04386

Clone

Cloning Information :

627019

Original Clone ID :

HR6324C-824-1150-15.3

Experimental

TargetTrack - Experimental data :

HR6324C

Organism

Species Specific ID:

1660

Genome

Chromosome :

Map Location :

1q25

Ensembl :

ENSG00000135829

Reagents

Labome :

DHX9-antibody

DHX9 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

DHX9 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)