DNASU Plasmid | TEC (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TEC

Gene Name: tec protein tyrosine kinase

Original Clone ID:

HR3504D-242-631-15.2

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00598179

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1176nts

Open reading frame: 14 to 1189

Reference Sequence Alignment

CCATCACAGCCATATGTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAA
GGCAGAGCAACTCCTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAG
TCAACCAGGCTTGTACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGG
TTTTAGGCATTATCATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGCTGA
AAAACATGCTTTTGGCTCCATTCCTGAGATTATTGAATATCATAAGCACAATGCAGCAGG
ACTTGTCACCAGGCTTCGGTACCCAGTTAGTGTGAAAGGGAAGAATGCACCCACCACTGC
AGGATTCAGCTATGAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGAGGGAATT
GGGAAGTGGACTGTTTGGAGTAGTGAGGCTTGGCAAATGGCGAGCCCAGTACGAAGTCGC
AATCAAAGCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGT
GATGATGAAACTGACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAA
ACCAATATACATTGTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACA
GAGACAAGGTCATTTCAGTAGAGACGTACTGCTGAGCATGTGTCAGGATGTGTGTGAAGG
GATGGAGTATCTGGAGAGAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCT
AGTAAGTGAGGCGGGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCTGGA
TGATCAGTACACAAGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGT
GTTTAATTACAGCCGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTG
GGAAGTATTCACGGAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAAC
CATGGTTACTCGAGGCCACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGCGTATGA
GGTGATGCTGAGATGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCTGCT
GCGCACAATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGAAGATAG

Protein sequence predicted based on nucleotide sequence

MLDQYEWYCRNMNRSKAEQLLRSEDKEGGFMVRDSSQPGLYTVSLYTKFGGEGSSGFRHY
HIKETTTSPKKYYLAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSVKGKNAPTTAGFSY
EKWEINPSELTFMRELGSGLFGVVRLGKWRAQYEVAIKAIREGAMCEEDFIEEAKVMMKL
THPKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDVLLSMCQDVCEGMEYL
ERNSFIHRDLAARNCLVSEAGVVKVSDFGMARYVLDDQYTSSSGAKFPVKWCPPEVFNYS
RFSSKSDVWSFGVLMWEVFTEGRMPFEKYTNYEVVTMVTRGHRLYQPKLASNYAYEVMLR
CWQEKPEGRPSFEDLLRTIDELVECEETFGR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g429a,V143V;a460g,K154E;

t1061c,V354A

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR3504D

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

TEC

Symbol Nomenclature :

TEC

SYNONYM :

PSCTK4

Designation :

tyrosine-protein kinase Tec

Full Nomenclature :

tec protein tyrosine kinase

GENEID :

7006

GI :

130977759

GenBank Accession :

NM_003215

HGNC :

11719

MIM :

600583

Vega :

OTTHUMG00000128623

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00598179      ------------------------------------------------------------
NM_003215.2       ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAG
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       ACATCGCCCTTAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTAC
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       TATGAGGGTCGAGCAGAGAAGAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAG
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       TGTGTGGAAATAGTGAAGAATGATGATGGTGTCATTCCCTGTCAAAATAAGTATCCATTT
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       CAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGTCCACAAAGCAGGGAC
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       CTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTATGATTAAATAT
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       CATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       CCCGGATGTGAAAAATACAATCTTTTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCA
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       CCAGAAACAAAGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGT
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       GAAGAAATCGTTGTAGCCATGTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTA
                                                                              

HsCD00598179      ------------------------------------------------------------
NM_003215.2       GAGAGAGGCCAAGAGTATCTCATTTTAGAAAAGAATGATGTTCATTGGTGGAGAGCAAGA
                                                                              

HsCD00598179      -----------------------------------------------CCATCACAGCCAT
NM_003215.2       GATAAATATGGGAATGAAGGATATATCCCAAGTAATTACGTAACGGGAAAGAAATCAAAC
                                                                 ..* .*.: ..* 

HsCD00598179      ATGTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGCAGAGCAACTC
NM_003215.2       AACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGCAGAGCAACTC
                  *: *********************************************************

HsCD00598179      CTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
NM_003215.2       CTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
                  ************************************************************

HsCD00598179      TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTAT
NM_003215.2       TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTAT
                  ************************************************************

HsCD00598179      CATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTT
NM_003215.2       CATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTT
                  ************************************************************

HsCD00598179      GGCTCCATTCCTGAGATTATTGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGG
NM_003215.2       GGCTCCATTCCTGAGATTATTGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGG
                  ************************************************************

HsCD00598179      CTTCGGTACCCAGTTAGTGTGAAAGGGAAGAATGCACCCACCACTGCAGGATTCAGCTAT
NM_003215.2       CTTCGGTACCCAGTTAGTGTGAAAGGGAAGAATGCACCCACCACTGCAGGATTCAGCTAT
                  ************************************************************

HsCD00598179      GAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGAGGGAATTGGGAAGTGGACTG
NM_003215.2       GAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGAGGGAATTGGGAAGTGGACTG
                  ************************************************************

HsCD00598179      TTTGGAGTAGTGAGGCTTGGCAAATGGCGAGCCCAGTACGAAGTCGCAATCAAAGCTATT
NM_003215.2       TTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAATCAAAGCTATT
                  ********.******************************.********************

HsCD00598179      CGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
NM_003215.2       CGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
                  ************************************************************

HsCD00598179      ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATT
NM_003215.2       ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATT
                  ************************************************************

HsCD00598179      GTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCAT
NM_003215.2       GTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCAT
                  ************************************************************

HsCD00598179      TTCAGTAGAGACGTACTGCTGAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTG
NM_003215.2       TTCAGTAGAGACGTACTGCTGAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTG
                  ************************************************************

HsCD00598179      GAGAGAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCTAGTAAGTGAGGCG
NM_003215.2       GAGAGAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCTAGTAAGTGAGGCG
                  ************************************************************

HsCD00598179      GGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCTGGATGATCAGTACACA
NM_003215.2       GGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCTGGATGATCAGTACACA
                  ************************************************************

HsCD00598179      AGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTTTAATTACAGC
NM_003215.2       AGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTTTAATTACAGC
                  ************************************************************

HsCD00598179      CGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
NM_003215.2       CGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
                  ************************************************************

HsCD00598179      GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGA
NM_003215.2       GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGA
                  ************************************************************

HsCD00598179      GGCCACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGCGTATGAGGTGATGCTGAGA
NM_003215.2       GGCCACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGA
                  **************************************** *******************

HsCD00598179      TGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGAT
NM_003215.2       TGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGAT
                  ************************************************************

HsCD00598179      GAACTAGTTGAATGTGAAGAAACTTTTGGAAGATAG
NM_003215.2       GAACTAGTTGAATGTGAAGAAACTTTTGGAAGATAA
                  ***********************************.

Pathways

NCI :

EPO signaling pathway

NCI :

Signaling events mediated by Stem cell factor receptor (c-Kit)

WikiPathway :

B Cell Receptor Signaling Pathway

WikiPathway :

Kit receptor signaling pathway

WikiPathway :

Prolactin Signaling Pathway

Protein

Structural Annotation Wiki :

HR3504D

UniProt :

P42680

HPRD :

02786

Clone

Cloning Information :

626646

Original Clone ID :

HR3504D-242-631-15.2

Experimental

TargetTrack - Experimental data :

HR3504D

Organism

Species Specific ID:

7006

Genome

Chromosome :

Map Location :

4p12

Ensembl :

ENSG00000135605

Reagents

Labome :

TEC-antibody

TEC (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

TEC (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)