DNASU Plasmid | RFC4 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RFC4

Gene Name: replication factor C (activator 1) 4, 37kDa

Original Clone ID:

HR2900E-38-283-15.9

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00598277

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 744nts

Open reading frame: 51 to 794

Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGTTCCCT
GGGTGGAAAAATATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTG
CAGTGCTGAAAAAATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCAC
CTGGAACTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAAC
TTTTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTC
GAGAGAAAGTGAAAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGC
CGTGTCCGCCTTTTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTC
AGGCAGCTTTAAGACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCT
GTAACTATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCA
AGCCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATG
TCAAAATTAGTGATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAA
AAGCCATTACATTTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGA
AAGTGATTACAGACATTGCTGAGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTG
CCTGTCAGAGTTAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGC
TGCTG

Protein sequence predicted based on nucleotide sequence

MVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELF
GPELFRLRVLELNASDERGIQVVREKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMT
SAAQAALRRTMEKESKTTRFCLICNYVSRIIEPLTSRCSKFRFKPLSDKIQQQRLLDIAK
KENVKISDEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEITEKVITDIAEVIPAEKIDG
VFAACQS*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g692a,G231E

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR2900E

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

RFC4

Symbol Nomenclature :

RFC4

SYNONYM :

A1; RFC37

Designation :

Full Nomenclature :

replication factor C (activator 1) 4, 37kDa

GENEID :

5984

GI :

31881687

GenBank Accession :

NM_181573

HGNC :

9972

MIM :

102577

Vega :

OTTHUMG00000156515

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00598277      --------CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAG------
NM_181573.2       ATGCAAGCATTTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGA
NM_002916.3       ATGCAAGCATTTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGA
                          .***.: **.*. * **.*.: .* .*::.***. *.* .:*..**      

HsCD00598277      --------------------------------------------CCATATGGTTCCCTGG
NM_181573.2       GGAGTAGCTGCCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGG
NM_002916.3       GGAGTAGCTGCCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGG
                                                              *.*:.  *********

HsCD00598277      GTGGAAAAATATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCA
NM_181573.2       GTGGAAAAATATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCA
NM_002916.3       GTGGAAAAATATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCA
                  ************************************************************

HsCD00598277      GTGCTGAAAAAATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCT
NM_181573.2       GTGCTGAAAAAATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCT
NM_002916.3       GTGCTGAAAAAATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCT
                  ************************************************************

HsCD00598277      GGAACTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTT
NM_181573.2       GGAACTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTT
NM_002916.3       GGAACTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTT
                  ************************************************************

HsCD00598277      TTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGA
NM_181573.2       TTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGA
NM_002916.3       TTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGA
                  ************************************************************

HsCD00598277      GAGAAAGTGAAAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCG
NM_181573.2       GAGAAAGTGAAAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCG
NM_002916.3       GAGAAAGTGAAAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCG
                  ************************************************************

HsCD00598277      TGTCCGCCTTTTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAG
NM_181573.2       TGTCCGCCTTTTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAG
NM_002916.3       TGTCCGCCTTTTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAG
                  ************************************************************

HsCD00598277      GCAGCTTTAAGACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGT
NM_181573.2       GCAGCTTTAAGACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGT
NM_002916.3       GCAGCTTTAAGACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGT
                  ************************************************************

HsCD00598277      AACTATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAG
NM_181573.2       AACTATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAG
NM_002916.3       AACTATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAG
                  ************************************************************

HsCD00598277      CCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTC
NM_181573.2       CCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTC
NM_002916.3       CCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTC
                  ************************************************************

HsCD00598277      AAAATTAGTGATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAA
NM_181573.2       AAAATTAGTGATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAA
NM_002916.3       AAAATTAGTGATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAA
                  ************************************************************

HsCD00598277      GCCATTACATTTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAA
NM_181573.2       GCCATTACATTTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAA
NM_002916.3       GCCATTACATTTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAA
                  ************************************************************

HsCD00598277      GTGATTACAGACATTGCTGAGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCC
NM_181573.2       GTGATTACAGACATTGCCGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCC
NM_002916.3       GTGATTACAGACATTGCCGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCC
                  ***************** *.****************************************

HsCD00598277      TGTCAGAGTTAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
NM_181573.2       TGTCAGAGTGGCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGT
NM_002916.3       TGTCAGAGTGGCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGT
                  ********* .     : .:*...**. :*.*:.:*  *.*... ::..*...* .*   

HsCD00598277      CTG---------------------------------------------------------
NM_181573.2       CATGCAGCAACTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCT
NM_002916.3       CATGCAGCAACTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCT
                  *:                                                          

HsCD00598277      ------------------------------------------------------------
NM_181573.2       GATAAACAGAAGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGAT
NM_002916.3       GATAAACAGAAGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGAT
                                                                              

HsCD00598277      ------------------------------------------------------------
NM_181573.2       GGTGCTGATGAACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCT
NM_002916.3       GGTGCTGATGAACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCT
                                                                              

HsCD00598277      ------------
NM_181573.2       CAGAATTGTTAA
NM_002916.3       CAGAATTGTTAA

Pathways

NCI :

ATR signaling pathway

NCI :

Fanconi anemia pathway

Panther :

DNA replication

Reactome :

Activation of ATR in response to replication stress

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Chromosome Maintenance

Reactome :

DNA Repair

Reactome :

DNA Replication

Reactome :

DNA strand elongation

Reactome :

Extension of Telomeres

Reactome :

G2/M Checkpoints

Reactome :

Gap-filling DNA repair synthesis and ligation in GG-NER

Reactome :

Gap-filling DNA repair synthesis and ligation in TC-NER

Reactome :

Global Genomic NER (GG-NER)

Reactome :

Lagging Strand Synthesis

Reactome :

Leading Strand Synthesis

Reactome :

Nucleotide Excision Repair

Reactome :

Polymerase switching

Reactome :

Polymerase switching on the C-strand of the telomere

Reactome :

Repair synthesis for gap-filling by DNA polymerase in TC-NER

Reactome :

Repair synthesis of patch ~27-30 bases long by DNA polymerase

Reactome :

S Phase

Reactome :

Synthesis of DNA

Reactome :

Telomere C-strand (Lagging Strand) Synthesis

Reactome :

Telomere Maintenance

Reactome :

Transcription-coupled NER (TC-NER)

WikiPathway :

DNA Replication

Protein

Structural Annotation Wiki :

HR2900E

UniProt :

P35249

HPRD :

00022

Clone

Cloning Information :

625960

Original Clone ID :

HR2900E-38-283-15.9

Experimental

TargetTrack - Experimental data :

HR2900E

Organism

Species Specific ID:

5984

Genome

Chromosome :

Map Location :

3q27

Ensembl :

ENSG00000163918

Reagents

Labome :

RFC4-antibody

RFC4 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

RFC4 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)