DNASU Plasmid | LONP1 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: LONP1

Gene Name: lon peptidase 1, mitochondrial

Original Clone ID:

HR6729-408-753-15.2

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00600527

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1044nts

Open reading frame: 51 to 1094

Reference Sequence Alignment

TTTAAGAAGGAGATATACCATGGGGCCATCACCATCACCATTACAGCCATATGGGCCTGG
AGAAGGACGACAAGGATGCCATCGAGGAGAAGTTCCGGGAGCGCCTGAAGGAGCTCGTGG
TCCCCAAGCACGTCATGGATGTTGTGGACGAGGAGCTGAGCAAGCTGGGCCTGCTGGACA
ACCACTCCTCGGAGTTCAATGTCACCCGCAACTACCTAGACTGGCTCACGTCCATCCCTT
GGGGCAAGTACAGCAACGAGAACCTGGACCTGGCGCGGGCACAGGCAGTGCTGGAGGAAG
ACCACTACGGCATGGAGGACGTCAAGAAACGCATCCTGGAGTTCATTGCCGTTAGCCAGC
TCCGCGGCTCCACCCAGGGCAAGATCCTCTGCTTCTATGGCCCCCCTGGCGTGGGTAAGA
CCAGCATTGCTCGCTCCATCGCCCGCGCCCTGAACCGAGAGTACTTCCGCTTCAGCGTCG
GGGGCATGACTGACGTGGCTGAGATCAAGGGCCACAGGCGGACCTACGTGGGCGCCATGC
CCGGGAAGATCATCCAGTGTTTGAAGAAGACCAAGACGGAGAACCCCCTGATCCTCATCG
ACGAGGTGGACAAGATCGGCCGAGGCTACCAGGGGGACCCGTCGTCGGCACTGCTGGAGC
TGCTGGACCCAGAGCAGAATGCCAACTTCCTGGACCACTACCTGGACGTGCCCGTGGACT
TGTCCAAGGTGCTGTTCATCTGCACGGCCAACGTCACGGACACTATCCCCGAGCCGCTGC
GAGACCGTATGGAGATGATCAACGTGTCGGGCTACGTGGCCCAGGAGAAGCTGGCCATTG
CGGAGCGCTACCTGCTGCCCCAGGCTCGCGCCCTGTGTGGCTTGGATGAGAGCAAGGCCA
AGCTGTCATCGGACGTGCTGACGCTGCTCATCAAGCAGTACTGCCGCGAGAGCGGTGTCC
GCAACCTGCAGAAGCAAGTGGAGAAGGTGTTACGGAAATCGGCCTACAAGATTGTCAGCG
GCGAGGCCGAGTCCGTGGAGGTGACGCCCGAGAACCTGCAGGACTTCGTGGGGAAGCCCG
TGTTCACCGTGTAGCTCGAGATCCGGCTGCTAACAAA

Protein sequence predicted based on nucleotide sequence

MGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLT
SIPWGKYSNENLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPG
VGKTSIARSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPL
ILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVTDTIP
EPLRDRMEMINVSGYVAQEKLAIAERYLLPQARALCGLDESKAKLSSDVLTLLIKQYCRE
SGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTV*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c714t,T238T;g805c,V269L

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in HR6729

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Protein

Clone

Experimental

Organism

Genome

Gene

Gene Symbol :

LONP1

Symbol Nomenclature :

LONP1

SYNONYM :

LON; LONP; LonHS; PIM1; PRSS15; hLON

Designation :

hLON ATP-dependent protease|lon protease homolog, mitochondrial|mitochondrial ATP-dependent protease Lon|mitochondrial lon protease-like protein|serine protease 15

Full Nomenclature :

lon peptidase 1, mitochondrial

GENEID :

9361

GenBank Accession :

NR_076392

HGNC :

9479

MIM :

605490

Vega :

OTTHUMG00000180504

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00600527        ------------------------------------------------------------
NM_004793.3         ATGGCGGCGAGCACTGGCTACGTGCGACTGTGGGGAGCGGCGCGGTGCTGGGTGCTGCGG
NM_001276479.1      ATGGCGGCGAGCACTGGCTACGTGCGACTGTGGGGAGCGGCGCGGTGCTGGGTGCTGCGG
NM_001276480.1      ------------------------------------------------------------
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         CGGCCGATGCTGGCCGCCGCCGGGGGGCGGGTTCCCACTGCAGCAGGAGCGTGGTTGCTC
NM_001276479.1      CGGCCGATGCTGGCCGCCGCCGGGGGGCGGGTTCCCACTGCAGCAGGAGCGTGGTTGCTC
NM_001276480.1      ------------------------------------------------------------
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         CGAGGCCAGCGGACCTGCGACGCCTCTCCTCCTTGGGCACTGTGGGGCCGAGGCCCGGCA
NM_001276479.1      CGAG--------------------------------------------------------
NM_001276480.1      ------------------------------------------------------------
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         ATTGGGGGCCAATGGCGGGGGTTTTGGGAAGCGAGCAGCCGCGGCGGAGGCGCATTCTCG
NM_001276479.1      ------------------------------------------------------------
NM_001276480.1      ------------------------------------------------------------
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GGGGGCGAGGACGCCTCCGAGGGCGGCGCGGAGGAAGGAGCCGGCGGCGCGGGGGGCAGC
NM_001276479.1      ------------------------------------------------------------
NM_001276480.1      ------------------------------------------------------------
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GCGGGCGCCGGGGAAGGCCCGGTCATAACGGCGCTCACGCCCATGACGATCCCCGATGTG
NM_001276479.1      ----------------GCCCGGTCATAACGGCGCTCACGCCCATGACGATCCCCGATGTG
NM_001276480.1      ------------------------------------------------------------
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         TTTCCGCACCTGCCGCTCATCGCCATCACCCGCAACCCGGTGTTCCCGCGCTTTATCAAG
NM_001276479.1      TTTCCGCACCTGCCGCTCATCGCCATCACCCGCAACCCGGTGTTCCCGCGCTTTATCAAG
NM_001276480.1      ------------------------------------------------------------
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         ATTATCGAGGTTAAAAATAAGAAGTTGGTTGAGCTGCTGAGAAGGAAAGTTCGTCTCGCC
NM_001276479.1      ATTATCGAGGTTAAAAATAAGAAGTTGGTTGAGCTGCTGAGAAGGAAAGTTCGTCTCGCC
NM_001276480.1      ------------------------------------------------------------
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         CAGCCTTATGTCGGCGTCTTTCTAAAGAGAGATGACAGCAATGAGTCGGATGTGGTCGAG
NM_001276479.1      CAGCCTTATGTCGGCGTCTTTCTAAAGAGAGATGACAGCAATGAGTCGGATGTGGTCGAG
NM_001276480.1      ------------------------------------------------------------
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         AGCCTGGATGAAATCTACCACACGGGGACGTTTGCCCAGATCCATGAGATGCAGGACCTT
NM_001276479.1      AGCCTGGATGAAATCTACCACACGGGGACGTTTGCCCAGATCCATGAGATGCAGGACCTT
NM_001276480.1      ------------------------------------------------ATGCAGGACCTT
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GGGGACAAGCTGCGCATGATCGTCATGGGACACAGAAGAGTCCATATCAGCAGACAGCTG
NM_001276479.1      GGGGACAAGCTGCGCATGATCGTCATGGGACACAGAAGAGTCCATATCAGCAGACAGCTG
NM_001276480.1      GGGGACAAGCTGCGCATGATCGTCATGGGACACAGAAGAGTCCATATCAGCAGACAGCTG
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GAGGTGGAGCCCGAGGAGCCGGAGGCGGAGAACAAGCACAAGCCCCGCAGGAAGTCAAAG
NM_001276479.1      GAGGTGGAGCCCGAGGAGCCGGAGGCGGAGAACAAGCACAAGCCCCGCAGGAAGTCAAAG
NM_001276480.1      GAGGTGGAGCCCGAGGAGCCGGAGGCGGAGAACAAGCACAAGCCCCGCAGGAAGTCAAAG
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         CGGGGCAAGAAGGAGGCGGAGGACGAGCTGAGCGCCAGGCACCCGGCGGAGCTGGCGATG
NM_001276479.1      CGGGGCAAGAAGGAGGCGGAGGACGAGCTGAGCGCCAGGCACCCGGCGGAGCTGGCGATG
NM_001276480.1      CGGGGCAAGAAGGAGGCGGAGGACGAGCTGAGCGCCAGGCACCCGGCGGAGCTGGCGATG
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GAGCCCACCCCTGAGCTCCCGGCTGAGGTGCTCATGGTGGAGGTAGAGAACGTTGTCCAC
NM_001276479.1      GAGCCCACCCCTGAGCTCCCGGCTGAGGTGCTCATGGTGGAGGTAGAGAACGTTGTCCAC
NM_001276480.1      GAGCCCACCCCTGAGCTCCCGGCTGAGGTGCTCATGGTGGAGGTAGAGAACGTTGTCCAC
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GAGGACTTCCAGGTCACGGAGGAGGTGAAAGCCCTGACTGCAGAGATCGTGAAGACCATC
NM_001276479.1      GAGGACTTCCAGGTCACGGAGGAGGTGAAAGCCCTGACTGCAGAGATCGTGAAGACCATC
NM_001276480.1      GAGGACTTCCAGGTCACGGAGGAGGTGAAAGCCCTGACTGCAGAGATCGTGAAGACCATC
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         CGGGACATCATTGCCTTGAACCCTCTCTACAGGGAGTCAGTGCTGCAGATGATGCAGGCT
NM_001276479.1      CGGGACATCATTGCCTTGAACCCTCTCTACAGGGAGTCAGTGCTGCAGATGATGCAGGCT
NM_001276480.1      CGGGACATCATTGCCTTGAACCCTCTCTACAGGGAGTCAGTGCTGCAGATGATGCAGGCT
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GGCCAGCGGGTGGTGGACAACCCCATCTACCTGAGCGACATGGGCGCCGCGCTCACCGGG
NM_001276479.1      GGCCAGCGGGTGGTGGACAACCCCATCTACCTGAGCGACATGGGCGCCGCGCTCACCGGG
NM_001276480.1      GGCCAGCGGGTGGTGGACAACCCCATCTACCTGAGCGACATGGGCGCCGCGCTCACCGGG
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GCCGAGTCCCATGAGCTGCAGGACGTCCTGGAAGAGACCAATATTCCTAAGCGGCTGTAC
NM_001276479.1      GCCGAGTCCCATGAGCTGCAGGACGTCCTGGAAGAGACCAATATTCCTAAGCGGCTGTAC
NM_001276480.1      GCCGAGTCCCATGAGCTGCAGGACGTCCTGGAAGAGACCAATATTCCTAAGCGGCTGTAC
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         AAGGCCCTCTCCCTGCTGAAGAAGGAATTTGAACTGAGCAAGCTGCAGCAGCGCCTGGGG
NM_001276479.1      AAGGCCCTCTCCCTGCTGAAGAAGGAATTTGAACTGAGCAAGCTGCAGCAGCGCCTGGGG
NM_001276480.1      AAGGCCCTCTCCCTGCTGAAGAAGGAATTTGAACTGAGCAAGCTGCAGCAGCGCCTGGGG
                                                                                

HsCD00600527        ----------------------------TTTAAGAAGGAGATATACCATGGGGCCATCAC
NM_004793.3         CGGGAGGTGGAGGAGAAGATCAAGCAGACCCACCGTAAGTACCTGCTGCAGGAGCAGCTA
NM_001276479.1      CGGGAGGTGGAGGAGAAGATCAAGCAGACCCACCGTAAGTACCTGCTGCAGGAGCAGCTA
NM_001276480.1      CGGGAGGTGGAGGAGAAGATCAAGCAGACCCACCGTAAGTACCTGCTGCAGGAGCAGCTA
                                                   *. .:... * .*.* . .**. ** *:.

HsCD00600527        CATCACCATTACAGCCATATGGGCCTGGAGAAGGACGACAAGGATGCCATCGAGGAGAAG
NM_004793.3         AAGATCATCAAGAAGGAGCTGGGCCTGGAGAAGGACGACAAGGATGCCATCGAGGAGAAG
NM_001276479.1      AAGATCATCAAGAAGGAGCTGGGCCTGGAGAAGGACGACAAGGATGCCATCGAGGAGAAG
NM_001276480.1      AAGATCATCAAGAAGGAGCTGGGCCTGGAGAAGGACGACAAGGATGCCATCGAGGAGAAG
                    .* .:*.: :* *.  * .*****************************************

HsCD00600527        TTCCGGGAGCGCCTGAAGGAGCTCGTGGTCCCCAAGCACGTCATGGATGTTGTGGACGAG
NM_004793.3         TTCCGGGAGCGCCTGAAGGAGCTCGTGGTCCCCAAGCACGTCATGGATGTTGTGGACGAG
NM_001276479.1      TTCCGGGAGCGCCTGAAGGAGCTCGTGGTCCCCAAGCACGTCATGGATGTTGTGGACGAG
NM_001276480.1      TTCCGGGAGCGCCTGAAGGAGCTCGTGGTCCCCAAGCACGTCATGGATGTTGTGGACGAG
                    ************************************************************

HsCD00600527        GAGCTGAGCAAGCTGGGCCTGCTGGACAACCACTCCTCGGAGTTCAATGTCACCCGCAAC
NM_004793.3         GAGCTGAGCAAGCTGGGCCTGCTGGACAACCACTCCTCGGAGTTCAATGTCACCCGCAAC
NM_001276479.1      GAGCTGAGCAAGCTGGGCCTGCTGGACAACCACTCCTCGGAGTTCAATGTCACCCGCAAC
NM_001276480.1      GAGCTGAGCAAGCTGGGCCTGCTGGACAACCACTCCTCGGAGTTCAATGTCACCCGCAAC
                    ************************************************************

HsCD00600527        TACCTAGACTGGCTCACGTCCATCCCTTGGGGCAAGTACAGCAACGAGAACCTGGACCTG
NM_004793.3         TACCTAGACTGGCTCACGTCCATCCCTTGGGGCAAGTACAGCAACGAGAACCTGGACCTG
NM_001276479.1      TACCTAGACTGGCTCACGTCCATCCCTTGGGGCAAGTACAGCAACGAGAACCTGGACCTG
NM_001276480.1      TACCTAGACTGGCTCACGTCCATCCCTTGGGGCAAGTACAGCAACGAGAACCTGGACCTG
                    ************************************************************

HsCD00600527        GCGCGGGCACAGGCAGTGCTGGAGGAAGACCACTACGGCATGGAGGACGTCAAGAAACGC
NM_004793.3         GCGCGGGCACAGGCAGTGCTGGAGGAAGACCACTACGGCATGGAGGACGTCAAGAAACGC
NM_001276479.1      GCGCGGGCACAGGCAGTGCTGGAGGAAGACCACTACGGCATGGAGGACGTCAAGAAACGC
NM_001276480.1      GCGCGGGCACAGGCAGTGCTGGAGGAAGACCACTACGGCATGGAGGACGTCAAGAAACGC
                    ************************************************************

HsCD00600527        ATCCTGGAGTTCATTGCCGTTAGCCAGCTCCGCGGCTCCACCCAGGGCAAGATCCTCTGC
NM_004793.3         ATCCTGGAGTTCATTGCCGTTAGCCAGCTCCGCGGCTCCACCCAGGGCAAGATCCTCTGC
NM_001276479.1      ATCCTGGAGTTCATTGCCGTTAGCCAGCTCCGCGGCTCCACCCAGGGCAAGATCCTCTGC
NM_001276480.1      ATCCTGGAGTTCATTGCCGTTAGCCAGCTCCGCGGCTCCACCCAGGGCAAGATCCTCTGC
                    ************************************************************

HsCD00600527        TTCTATGGCCCCCCTGGCGTGGGTAAGACCAGCATTGCTCGCTCCATCGCCCGCGCCCTG
NM_004793.3         TTCTATGGCCCCCCTGGCGTGGGTAAGACCAGCATTGCTCGCTCCATCGCCCGCGCCCTG
NM_001276479.1      TTCTATGGCCCCCCTGGCGTGGGTAAGACCAGCATTGCTCGCTCCATCGCCCGCGCCCTG
NM_001276480.1      TTCTATGGCCCCCCTGGCGTGGGTAAGACCAGCATTGCTCGCTCCATCGCCCGCGCCCTG
                    ************************************************************

HsCD00600527        AACCGAGAGTACTTCCGCTTCAGCGTCGGGGGCATGACTGACGTGGCTGAGATCAAGGGC
NM_004793.3         AACCGAGAGTACTTCCGCTTCAGCGTCGGGGGCATGACTGACGTGGCTGAGATCAAGGGC
NM_001276479.1      AACCGAGAGTACTTCCGCTTCAGCGTCGGGGGCATGACTGACGTGGCTGAGATCAAGGGC
NM_001276480.1      AACCGAGAGTACTTCCGCTTCAGCGTCGGGGGCATGACTGACGTGGCTGAGATCAAGGGC
                    ************************************************************

HsCD00600527        CACAGGCGGACCTACGTGGGCGCCATGCCCGGGAAGATCATCCAGTGTTTGAAGAAGACC
NM_004793.3         CACAGGCGGACCTACGTGGGCGCCATGCCCGGGAAGATCATCCAGTGTTTGAAGAAGACC
NM_001276479.1      CACAGGCGGACCTACGTGGGCGCCATGCCCGGGAAGATCATCCAGTGTTTGAAGAAGACC
NM_001276480.1      CACAGGCGGACCTACGTGGGCGCCATGCCCGGGAAGATCATCCAGTGTTTGAAGAAGACC
                    ************************************************************

HsCD00600527        AAGACGGAGAACCCCCTGATCCTCATCGACGAGGTGGACAAGATCGGCCGAGGCTACCAG
NM_004793.3         AAGACGGAGAACCCCCTGATCCTCATCGACGAGGTGGACAAGATCGGCCGAGGCTACCAG
NM_001276479.1      AAGACGGAGAACCCCCTGATCCTCATCGACGAGGTGGACAAGATCGGCCGAGGCTACCAG
NM_001276480.1      AAGACGGAGAACCCCCTGATCCTCATCGACGAGGTGGACAAGATCGGCCGAGGCTACCAG
                    ************************************************************

HsCD00600527        GGGGACCCGTCGTCGGCACTGCTGGAGCTGCTGGACCCAGAGCAGAATGCCAACTTCCTG
NM_004793.3         GGGGACCCGTCGTCGGCACTGCTGGAGCTGCTGGACCCAGAGCAGAATGCCAACTTCCTG
NM_001276479.1      GGGGACCCGTCGTCGGCACTGCTGGAGCTGCTGGACCCAGAGCAGAATGCCAACTTCCTG
NM_001276480.1      GGGGACCCGTCGTCGGCACTGCTGGAGCTGCTGGACCCAGAGCAGAATGCCAACTTCCTG
                    ************************************************************

HsCD00600527        GACCACTACCTGGACGTGCCCGTGGACTTGTCCAAGGTGCTGTTCATCTGCACGGCCAAC
NM_004793.3         GACCACTACCTGGACGTGCCCGTGGACTTGTCCAAGGTGCTGTTCATCTGCACGGCCAAC
NM_001276479.1      GACCACTACCTGGACGTGCCCGTGGACTTGTCCAAGGTGCTGTTCATCTGCACGGCCAAC
NM_001276480.1      GACCACTACCTGGACGTGCCCGTGGACTTGTCCAAGGTGCTGTTCATCTGCACGGCCAAC
                    ************************************************************

HsCD00600527        GTCACGGACACTATCCCCGAGCCGCTGCGAGACCGTATGGAGATGATCAACGTGTCGGGC
NM_004793.3         GTCACGGACACCATCCCCGAGCCGCTGCGAGACCGTATGGAGATGATCAACGTGTCGGGC
NM_001276479.1      GTCACGGACACCATCCCCGAGCCGCTGCGAGACCGTATGGAGATGATCAACGTGTCGGGC
NM_001276480.1      GTCACGGACACCATCCCCGAGCCGCTGCGAGACCGTATGGAGATGATCAACGTGTCGGGC
                    *********** ************************************************

HsCD00600527        TACGTGGCCCAGGAGAAGCTGGCCATTGCGGAGCGCTACCTGCTGCCCCAGGCTCGCGCC
NM_004793.3         TACGTGGCCCAGGAGAAGCTGGCCATTGCGGAGCGCTACCTGGTGCCCCAGGCTCGCGCC
NM_001276479.1      TACGTGGCCCAGGAGAAGCTGGCCATTGCGGAGCGCTACCTGGTGCCCCAGGCTCGCGCC
NM_001276480.1      TACGTGGCCCAGGAGAAGCTGGCCATTGCGGAGCGCTACCTGGTGCCCCAGGCTCGCGCC
                    ****************************************** *****************

HsCD00600527        CTGTGTGGCTTGGATGAGAGCAAGGCCAAGCTGTCATCGGACGTGCTGACGCTGCTCATC
NM_004793.3         CTGTGTGGCTTGGATGAGAGCAAGGCCAAGCTGTCATCGGACGTGCTGACGCTGCTCATC
NM_001276479.1      CTGTGTGGCTTGGATGAGAGCAAGGCCAAGCTGTCATCGGACGTGCTGACGCTGCTCATC
NM_001276480.1      CTGTGTGGCTTGGATGAGAGCAAGGCCAAGCTGTCATCGGACGTGCTGACGCTGCTCATC
                    ************************************************************

HsCD00600527        AAGCAGTACTGCCGCGAGAGCGGTGTCCGCAACCTGCAGAAGCAAGTGGAGAAGGTGTTA
NM_004793.3         AAGCAGTACTGCCGCGAGAGCGGTGTCCGCAACCTGCAGAAGCAAGTGGAGAAGGTGTTA
NM_001276479.1      AAGCAGTACTGCCGCGAGAGCGGTGTCCGCAACCTGCAGAAGCAAGTGGAGAAGGTGTTA
NM_001276480.1      AAGCAGTACTGCCGCGAGAGCGGTGTCCGCAACCTGCAGAAGCAAGTGGAGAAGGTGTTA
                    ************************************************************

HsCD00600527        CGGAAATCGGCCTACAAGATTGTCAGCGGCGAGGCCGAGTCCGTGGAGGTGACGCCCGAG
NM_004793.3         CGGAAATCGGCCTACAAGATTGTCAGCGGCGAGGCCGAGTCCGTGGAGGTGACGCCCGAG
NM_001276479.1      CGGAAATCGGCCTACAAGATTGTCAGCGGCGAGGCCGAGTCCGTGGAGGTGACGCCCGAG
NM_001276480.1      CGGAAATCGGCCTACAAGATTGTCAGCGGCGAGGCCGAGTCCGTGGAGGTGACGCCCGAG
                    ************************************************************

HsCD00600527        AACCTGCAGGACTTCGTGGGGAAGCCCGTGTTCACCGTGTAGCTCGAGATCCGGCTGCTA
NM_004793.3         AACCTGCAGGACTTCGTGGGGAAGCCCGTGTTCACCGTGGAGCGCATGTATGACGTGACA
NM_001276479.1      AACCTGCAGGACTTCGTGGGGAAGCCCGTGTTCACCGTGGAGCGCATGTATGACGTGACA
NM_001276480.1      AACCTGCAGGACTTCGTGGGGAAGCCCGTGTTCACCGTGGAGCGCATGTATGACGTGACA
                    *************************************** *** *.:*::  .  **. *

HsCD00600527        ACAAA-------------------------------------------------------
NM_004793.3         CCGCCCGGCGTGGTCATGGGGCTGGCCTGGACCGCAATGGGAGGCTCCACGCTGTTTGTG
NM_001276479.1      CCGCCCGGCGTGGTCATGGGGCTGGCCTGGACCGCAATGGGAGGCTCCACGCTGTTTGTG
NM_001276480.1      CCGCCCGGCGTGGTCATGGGGCTGGCCTGGACCGCAATGGGAGGCTCCACGCTGTTTGTG
                    .*...                                                       

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GAGACATCCCTGAGACGGCCACAGGACAAGGATGCCAAGGGTGACAAGGATGGCAGCCTG
NM_001276479.1      GAGACATCCCTGAGACGGCCACAGGACAAGGATGCCAAGGGTGACAAGGATGGCAGCCTG
NM_001276480.1      GAGACATCCCTGAGACGGCCACAGGACAAGGATGCCAAGGGTGACAAGGATGGCAGCCTG
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GAGGTGACAGGCCAGCTGGGGGAGGTGATGAAGGAGAGCGCCCGCATAGCCTACACCTTC
NM_001276479.1      GAGGTGACAGGCCAGCTGGGGGAGGTGATGAAGGAGAGCGCCCGCATAGCCTACACCTTC
NM_001276480.1      GAGGTGACAGGCCAGCTGGGGGAGGTGATGAAGGAGAGCGCCCGCATAGCCTACACCTTC
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GCCAGAGCCTTCCTCATGCAGCACGCCCCCGCCAATGACTACCTGGTGACCTCACACATC
NM_001276479.1      GCCAGAGCCTTCCTCATGCAGCACGCCCCCGCCAATGACTACCTGGTGACCTCACACATC
NM_001276480.1      GCCAGAGCCTTCCTCATGCAGCACGCCCCCGCCAATGACTACCTGGTGACCTCACACATC
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         CACCTGCATGTGCCCGAGGGCGCCACCCCCAAGGACGGCCCAAGCGCAGGCTGCACCATC
NM_001276479.1      CACCTGCATGTGCCCGAGGGCGCCACCCCCAAGGACGGCCCAAGCGCAGGCTGCACCATC
NM_001276480.1      CACCTGCATGTGCCCGAGGGCGCCACCCCCAAGGACGGCCCAAGCGCAGGCTGCACCATC
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GTCACGGCCCTGCTGTCCCTGGCCATGGGCAGGCCTGTCCGGCAGAATCTGGCCATGACT
NM_001276479.1      GTCACGGCCCTGCTGTCCCTGGCCATGGGCAGGCCTGTCCGGCAGAATCTGGCCATGACT
NM_001276480.1      GTCACGGCCCTGCTGTCCCTGGCCATGGGCAGGCCTGTCCGGCAGAATCTGGCCATGACT
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GGCGAAGTCTCCCTCACGGGCAAGATCCTGCCTGTTGGTGGCATCAAGGAGAAGACCATT
NM_001276479.1      GGCGAAGTCTCCCTCACGGGCAAGATCCTGCCTGTTGGTGGCATCAAGGAGAAGACCATT
NM_001276480.1      GGCGAAGTCTCCCTCACGGGCAAGATCCTGCCTGTTGGTGGCATCAAGGAGAAGACCATT
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GCGGCCAAGCGCGCAGGGGTGACGTGCATCGTCCTGCCAGCCGAGAACAAGAAGGACTTC
NM_001276479.1      GCGGCCAAGCGCGCAGGGGTGACGTGCATCGTCCTGCCAGCCGAGAACAAGAAGGACTTC
NM_001276480.1      GCGGCCAAGCGCGCAGGGGTGACGTGCATCGTCCTGCCAGCCGAGAACAAGAAGGACTTC
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         TACGACCTGGCAGCCTTCATCACCGAGGGCCTGGAGGTGCACTTCGTGGAACACTACCGG
NM_001276479.1      TACGACCTGGCAGCCTTCATCACCGAGGGCCTGGAGGTGCACTTCGTGGAACACTACCGG
NM_001276480.1      TACGACCTGGCAGCCTTCATCACCGAGGGCCTGGAGGTGCACTTCGTGGAACACTACCGG
                                                                                

HsCD00600527        ------------------------------------------------------------
NM_004793.3         GAGATCTTCGACATCGCCTTCCCGGACGAGCAGGCAGAGGCGCTGGCCGTGGAACGGTGA
NM_001276479.1      GAGATCTTCGACATCGCCTTCCCGGACGAGCAGGCAGAGGCGCTGGCCGTGGAACGGTGA
NM_001276480.1      GAGATCTTCGACATCGCCTTCCCGGACGAGCAGGCAGAGGCGCTGGCCGTGGAACGGTGA

Protein

Structural Annotation Wiki :

HR6729

UniProt :

P36776

HPRD :

05689

PDBe Mol* Structure Viewer

Clone

Cloning Information :

628684

Original Clone ID :

HR6729-408-753-15.2

Experimental

TargetTrack - Experimental data :

HR6729

Organism

Species Specific ID:

9361

Genome

Chromosome :

Map Location :

19p13.2

Ensembl :

ENSG00000196365

LONP1 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

PDBe Mol* Structure Viewer

Clone

Experimental

Organism

Genome

LONP1 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)