DNASU Plasmid | VPS4 (S. cerevisiae ) in pRS416 (Yeast centromere shuttle vector)

Explanation of Terms

Gene:

Gene Symbol: VPS4

Gene Name: AAA family ATPase VPS4

Original Clone ID:

None

Keyword:

Mutation:a1054g; g1055c, Mutation:R352A

Species:

Saccharomyces cerevisiae

Type:

cDNA

Vector Name:

pRS416

Format:

CLOSED

Source:

University of Utah

Description:

None

Comments:

pRS416+Vps4p(R352A)

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

University of Utah
Wesley I. Sundquist
Mary Anne Karren

ScCD00604354

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 1314nts

Open reading frame: 1 to 1314

ATGAGCACGGGAGATTTTTTAACTAAGGGAATAGAACTGGTTCAAAAGGCCATTGACCTG
GACACAGCCACGCAGTATGAGGAGGCGTATACAGCATACTATAACGGATTAGATTATTTG
ATGTTGGCTTTGAAATACGAGAAAAACCCTAAGTCGAAGGATTTGATAAGAGCGAAGTTT
ACAGAGTATCTAAACCGTGCAGAGCAGTTGAAAAAGCACTTGGAAAGTGAAGAGGCAAAC
GCGGCAAAGAAATCTCCTAGCGCTGGTAGTGGTTCCAACGGTGGTAATAAGAAAATCTCT
CAGGAAGAGGGTGAGGATAATGGTGGAGAAGACAACAAAAAGCTAAGAGGTGCCTTATCG
AGTGCTATTTTGTCTGAGAAGCCCAATGTTAAGTGGGAAGACGTGGCTGGACTGGAAGGA
GCCAAAGAGGCCCTTAAAGAAGCCGTTATTTTACCTGTCAAGTTCCCACATTTGTTCAAA
GGTAACCGTAAGCCCACTTCGGGAATCTTATTGTACGGACCACCAGGTACAGGTAAATCA
TATTTGGCAAAAGCAGTGGCCACCGAGGCCAATTCTACCTTTTTCAGTGTTAGTTCCAGT
GATTTGGTTTCTAAGTGGATGGGTGAATCTGAAAAACTTGTCAAACAGTTATTTGCGATG
GCAAGAGAAAATAAACCTTCTATTATTTTTATAGATGAAGTGGATGCGCTAACAGGTACC
AGAGGAGAAGGAGAGAGCGAAGCAAGTAGAAGAATTAAAACAGAATTATTGGTACAAATG
AATGGTGTTGGGAATGACTCCCAAGGTGTTTTGGTCCTTGGCGCAACAAATATCCCATGG
CAACTGGACAGTGCCATCAGGAGAAGATTTGAAAGAAGAATATATATACCATTACCAGAT
TTGGCGGCAAGAACCACCATGTTCGAGATTAACGTCGGCGACACTCCATGCGTATTGACC
AAGGAAGATTATAGAACTTTAGGTGCAATGACTGAAGGTTATTCCGGGAGTGATATTGCT
GTAGTGGTGAAAGATGCGCTAATGCAACCGATAGCAAAAATTCAAAGCGCGACACATTTC
AAAGATGTCTCTACGGAGGACGACGAAACAAGAAAACTAACACCATGCTCTCCAGGTGAT
GATGGCGCCATCGAGATGAGTTGGACGGATATTGAAGCTGATGAATTAAAAGAACCAGAT
CTCACCATAAAGGATTTCTTAAAGGCTATCAAATCGACGAGACCTACCGTGAATGAGGAT
GACTTGCTGAAGCAAGAACAGTTCACTAGAGATTTTGGTCAAGAAGGTAACTAA

Protein sequence predicted based on nucleotide sequence

MSTGDFLTKGIELVQKAIDLDTATQYEEAYTAYYNGLDYLMLALKYEKNPKSKDLIRAKF
TEYLNRAEQLKKHLESEEANAAKKSPSAGSGSNGGNKKISQEEGEDNGGEDNKKLRGALS
SAILSEKPNVKWEDVAGLEGAKEALKEAVILPVKFPHLFKGNRKPTSGILLYGPPGTGKS
YLAKAVATEANSTFFSVSSSDLVSKWMGESEKLVKQLFAMARENKPSIIFIDEVDALTGT
RGEGESEASRRIKTELLVQMNGVGNDSQGVLVLGATNIPWQLDSAIRRRFERRIYIPLPD
LAARTTMFEINVGDTPCVLTKEDYRTLGAMTEGYSGSDIAVVVKDALMQPIAKIQSATHF
KDVSTEDDETRKLTPCSPGDDGAIEMSWTDIEADELKEPDLTIKDFLKAIKSTRPTVNED
DLLKQEQFTRDFGQEGN*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

End read sequencing of cDNA insert

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: yeast Marker: URA
Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pRS416

Synonyms:

Sequencing Primer:

Forward: Unknown

Reverse: Unknown

Description:

Yeast centromere shuttle plasmid with a T7 promoter; ampicillin resistance in bacteria, URA3 resistance in yeast; restriction enzyme cloning

Comments:

Restriction enzymes BamHI, Sal1 and be used to clone into this vector

Size (bp):

4898

Parent Vector:

None

Properties:

multiple cloning site, yeast shuttle

Author Name:

Stratagene

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATACCACAGCTTTTCAATTCAATTCATCATTTTTTTTTTATTCTTTTTTTTGATTTCGGTTTCTTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAATCATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAAAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTATTACCCTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAGGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGGTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGGGTCCTTTTCATCACGTGCTATAAAAATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAACAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGTGTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTTATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGGCAAGCGATCCGTCCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

f1 origin

1483

1798

bacterial origin

ColE

ColE1 bacterial origin

2547

3229

bacterial origin

M13 Ori

M13 origin

1351

1806

primer

M13 forward

M13 forward sequencing primer

1951

1966

primer

M13 reverse

M13 reverse sequencing primer

2162

2179

promoter

T7 promoter

1976

1996

promoter

Ura3 promoter

302

331

selectable marker

AmpR

ampicillin resistance gene

3327

3986

selectable marker

Ura3

417

1220

trxn regulatory element

lac operator

2185

2207

yeast centromere

CEN6

CEN6-ARS4 yeast centromere

4316

4834

Gene

Clone

Organism

Genome

Gene

Gene Symbol :

VPS4

SYNONYM :

CSC1; DID6; END13; GRD13; VPL4; VPT10

GENEID :

856303

Locus Tag :

YPR173C

GenBank Accession :

NM_001184270

Yeast Genome Database :

S000006377

Target GenBank:

NM_001184270

Clone

Cloning Information :

664619

Organism

Species Specific ID:

856303

Genome

Chromosome :

XVI