Gene:
Gene Symbol:
S1PR1
Gene Name:
sphingosine-1-phosphate receptor 1
Original Clone ID:
FLH271235.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617897
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1149nts
Open reading frame: 249 to 1397
GGGGAGTCGGGGGCAGCAGCAAGATGCGAAGCGAGCCGTACAGATCCCGGGCTCTCCGAA
CGCAACTTCGCCCTGCTTGAGCGAGGCTGCGGTTTCCGAGGCCCTCTCCAGCCAAGGAAA
AGCTACACAAAAAGCCTGGATCACTCATCGAACCACCCCTGAAGCCAGTGAAGGCTCTCT
CGCCTCGCCCTCTAGCGTTCGTCTGGAGTAGCGCCACCCCGGCTTCCTGGGGACACAGGT
TTGGCACCATGGGGCCCACCAGCGTCCCGCTGGTCAAGGCCCACCGCAGCTCGGTCTCTG
ACTACGTCAACTATGATATCATCGTCCGGCATTACAACTACACGGGAAAGCTGAATATCA
GCGCGGACAAGGAGAACAGCATTAAACTGACCTCGGTGGTGTTCATTCTCATCTGCTGCT
TTATCATCCTGGAGAACATCTTTGTCTTGCTGACCATTTGGAAAACCAAGAAATTCCACC
GACCCATGTACTATTTTATTGGCAATCTGGCCCTCTCAGACCTGTTGGCAGGAGTAGCCT
ACACAGCTAACCTGCTCTTGTCTGGGGCCACCACCTACAAGCTCACTCCCGCCCAGTGGT
TTCTGCGGGAAGGGAGTATGTTTGTGGCCCTGTCAGCCTCCGTGTTCAGTCTCCTCGCCA
TCGCCATTGAGCGCTATATCACAATGCTGAAAATGAAACTCCACAACGGGAGCAATAACT
TCCGCCTCTTCCTGCTAATCAGCGCCTGCTGGGTCATCTCCCTCATCCTGGGTGGCCTGC
CTATCATGGGCTGGAACTGCATCAGTGCGCTGTCCAGCTGCTCCACCGTGCTGCCGCTCT
ACCACAAGCACTATATCCTCTTCTGCACCACGGTCTTCACTCTGCTTCTGCTCTCCATCG
TCATTCTGTACTGCAGAATCTACTCCTTGGTCAGGACTCGGAGCCGCCGCCTGACGTTCC
GCAAGAACATTTCCAAGGCCAGCCGCAGCTCTGAGAAGTCGCTGGCGCTGCTCAAGACCG
TAATTATCGTCCTGAGCGTCTTCATCGCCTGCTGGGCACCGCTCTTCATCCTGCTCCTGC
TGGATGTGGGCTGCAAGGTGAAGACCTGTGACATCCTCTTCAGAGCGGAGTACTTCCTGG
TGTTAGCTGTGCTCAACTCCGGCACCAACCCCATCATTTACACTCTGACCAACAAGGAGA
TGCGTCGGGCCTTCATCCGGATCATGTCCTGCTGCAAGTGCCCGAGCGGAGACTCTGCTG
GCAAATTCAAGCGACCCATCATCGCCGGCATGGAATTCAGCCGCAGCAAATCGGACAATT
CCTCCCACCCCCAGAAAGACGAAGGGGACAACCCAGAGACCATTATGTCTTCTGGAAACG
TCAACTCTTCTTCCTAGAACTGGAAGCTGTCCACCCACCGGAAGCGCTCTTTACTTGGTC
GCTGGCCACCCCAGTGTTTGGAAAAAAATCTCTGGGCTTCGACTGCTGCCAGGGAGGAGC
TGCTGCAAGCCAGAGGGAGGAAGGGGGAGAATACGAACAGCCTGGTGGTGTCGGGTGTTG
GTGGGTAGAGTTAGTTCCTGTGAACAATGCACTGGGAAGGGTGGAGATCAGGTCCCGGCC
TGGAATATATTTTCTACCCCCCTGGAGCTTTGATTTTGCACTGAGCCAAAGGTCTAGCAT
TGTCAAGCTCCTAAAGGGTTCATTTGGCCCCTCCTCAAAGACTAATGTCCCCATGTGAAA
GCGTCTCTTTGTCTGGAGCTTTGAGGAGATGTTTTCCTTCACTTTAGTTTCAAACCCAAG
TGAGTGTGTGCACTTCTGCTTCTTTAGGGATGCCCTGTACATCCCACACCCCACCCTCCC
TTCCCTTCATACCCCTCCTCAACGTTCTTTTACTTTATACTTTAACTACCTGAGAGTTAT
CAGAGCTGGGGTTGTGGAATGATCGATCATCTATAGCAAATAGGCTATGTTGAGTACGTA
GGCTGTGGGAAGATGAAGATGGTTTGGAGGTGTAAAACAATGTCCTTCGCTGAGGCCAAA
GTTTCCATGTAAGCGGGATCCGTTTTTTGGAATTTGGTTGAAGTCACTTTGATTTCTTTA
AAAAACATCTTTTCAATGAAATGTGTTACCATTTCATATCCATTGAAGCCGAAATCTGCA
TAAGGAAGCCCACTTTATCTAAATGATATTAGCCAGGATCCTTGGTGTCCTAGGAGAAAC
AGACAAGCAAAACAAAGTGAAAACCGAATGGATTAACTTTTGCAAACCAAGGGAGATTTC
TTAGCAAATGAGTCTAACAAATATGACATCTGTCTTTGGCACTTTTGTTGATGTTTATTT
CAGAATGTTGTGTGATTCATTTCAAGCAACAACATGGTTGTATTTTGTTGTGTTAAAAGT
ACTTTTCTTGATTTTTGAATGTATTTGTTTCAGCAGAAGTCATTTTATTGGATTTTTCTA
ACCCGTGTTAACACCATTGAATGTGTATTTCTTAAGAAAATACCACCCTCTTGTGCCCTT
AAAAGCATTACTTTAACTGGTAGGGAACGCCAGAAACTTTTCAGTCCAGCTATTCATTAG
ATAGTAATTGAAGATATGTATAAATATTACAAAGAATAAAAATATATTACTGTCTCTTTA
GTATGGTTTTCAGTGCAATTAAACCGAGAGATGTCTTGTTTTTTTAAAAAGAATAGTATT
TAATAGGTTTCTGACTTTTGTGGATCATTTTGCACATAGCTTTATCAACTTTTAAACATT
AATAAACTGATTTTTTTAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MGPTSVPLVKAHRSSVSDYVNYDIIVRHYNYTGKLNISADKENSIKLTSVVFILICCFII LENIFVLLTIWKTKKFHRPMYYFIGNLALSDLLAGVAYTANLLLSGATTYKLTPAQWFLR EGSMFVALSASVFSLLAIAIERYITMLKMKLHNGSNNFRLFLLISACWVISLILGGLPIM GWNCISALSSCSTVLPLYHKHYILFCTTVFTLLLLSIVILYCRIYSLVRTRSRRLTFRKN ISKASRSSEKSLALLKTVIIVLSVFIACWAPLFILLLLDVGCKVKTCDILFRAEYFLVLA VLNSGTNPIIYTLTNKEMRRAFIRIMSCCKCPSGDSAGKFKRPIIAGMEFSRSKSDNSSH PQKDEGDNPETIMSSGNVNSSS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
