DNASU Plasmid | PAK1 (H. sapiens )<br>In pLDNT7_nFLAG (Flag-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: PAK1

Gene Name: p21 protein (Cdc42/Rac)-activated kinase 1

Original Clone ID:

FLH231605.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLDNT7_nFLAG

Format:

CLOSED

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00617905

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1638nts

Open reading frame: 359 to 1996

AGCCCCGCGCTTTCGTGAGCCCCCTCGAGGAACCTGGTCTCCGCATCCAGTTACCACCTC
CTGCCTCAGAGGCCATCTGAGCCCTTCGCACCTCGCCCCTCAGTCCCCCCTTCCCCCCCC
GCCCGCGTCGCCTCGCTCCCTCCCGCCCCCCCATCATCCCTTCCCTCGCAGTTCCCCTGT
CCTGAGGGGAGCCCCGCCACGGGCAGCGCGGCGGCGGCGGCAGGAGGGAGAAAGTGAAGC
GGTAGCTCGCGCACACTCGCGCCCTCACTCCCGGCTAGGCGGCACCCACCGCCGGGAGGA
GGAGGAGGAGCCGAGAGGAGCTGAGCGAGCGCGGAAGTAGCTGCTGCTGGTGGTGACAAT
GTCAAATAACGGCCTAGACATTCAAGACAAACCCCCAGCCCCTCCGATGAGAAATACCAG
CACTATGATTGGAGCCGGCAGCAAAGATGCTGGAACCCTAAACCATGGTTCTAAACCTCT
GCCTCCAAACCCAGAGGAGAAGAAAAAGAAGGACCGATTTTACCGATCCATTTTACCTGG
AGATAAAACAAATAAAAAGAAAGAGAAAGAGCGGCCAGAGATTTCTCTCCCTTCAGATTT
TGAACACACAATTCATGTCGGTTTTGATGCTGTCACAGGGGAGTTTACGGGAATGCCAGA
GCAGTGGGCCCGCTTGCTTCAGACATCAAATATCACTAAGTCGGAGCAGAAGAAAAACCC
GCAGGCTGTTCTGGATGTGTTGGAGTTTTACAACTCGAAGAAGACATCCAACAGCCAGAA
ATACATGAGCTTTACAGATAAGTCAGCTGAGGATTACAATTCTTCTAATGCCTTGAATGT
GAAGGCTGTGTCTGAGACTCCTGCAGTGCCACCAGTTTCAGAAGATGAGGATGATGATGA
TGATGATGCTACCCCACCACCAGTGATTGCTCCACGCCCAGAGCACACAAAATCTGTATA
CACACGGTCTGTGATTGAACCACTTCCTGTCACTCCAACTCGGGACGTGGCTACATCTCC
CATTTCACCTACTGAAAATAACACCACTCCACCAGATGCTTTGACCCGGAATACTGAGAA
GCAGAAGAAGAAGCCTAAAATGTCTGATGAGGAGATCTTGGAGAAATTACGAAGCATAGT
GAGTGTGGGCGATCCTAAGAAGAAATATACACGGTTTGAGAAGATTGGACAAGGTGCTTC
AGGCACCGTGTACACAGCAATGGATGTGGCCACAGGACAGGAGGTGGCCATTAAGCAGAT
GAATCTTCAGCAGCAGCCCAAGAAAGAGCTGATTATTAATGAGATCCTGGTCATGAGGGA
AAACAAGAACCCAAACATTGTGAATTACTTGGACAGTTACCTCGTGGGAGATGAGCTGTG
GGTTGTTATGGAATACTTGGCTGGAGGCTCCTTGACAGATGTGGTGACAGAAACTTGCAT
GGATGAAGGCCAAATTGCAGCTGTGTGCCGTGAGTGTCTGCAGGCTCTGGAGTTCTTGCA
TTCGAACCAGGTCATTCACAGAGACATCAAGAGTGACAATATTCTGTTGGGAATGGATGG
CTCTGTCAAGCTAACTGACTTTGGATTCTGTGCACAGATAACCCCAGAGCAGAGCAAACG
GAGCACCATGGTAGGAACCCCATACTGGATGGCACCAGAGGTTGTGACACGAAAGGCCTA
TGGGCCCAAGGTTGACATCTGGTCCCTGGGCATCATGGCCATCGAAATGATTGAAGGGGA
GCCTCCATACCTCAATGAAAACCCTCTGAGAGCCTTGTACCTCATTGCCACCAATGGGAC
CCCAGAACTTCAGAACCCAGAGAAGCTGTCAGCTATCTTCCGGGACTTTCTGAACCGCTG
TCTCGAGATGGATGTGGAGAAGAGAGGTTCAGCTAAAGAGCTGCTACAGCATCAATTCCT
GAAGATTGCCAAGCCCCTCTCCAGCCTCACTCCACTGATTGCTGCAGCTAAGGAGGCAAC
AAAGAACAATCACTAAAACCACACTCACCCCAGCCTCATTGTGCCAAGCCTTCTGTGAGA
TAAATGCACATTTCAGAAATTCCAACTCCTGATGCCCTCTTCTCCTTGCCTTGCTTCTCC
CATTTCCTGATCTAGCACTCCTCAAGACTTTGATCCTTGGAAACCGTGTGTCCAGCATTG
AAGAGAACTGCAACTGAATGACTAATCAGATGATGGCCATTTCTAAATAAGGAATTTCCT
CCCAATTCATGGATATGAGGGTGGTTTATGATTAAGGGTTTATATAAATAAATGTTTCTA
GTCTTCCGTGTGTCAAAATCCTCACCTCCTTCATAACCATCTCCCACAATTAATTCTTGA
CTATATAAATTTATGGTTTGATAATATTATCAATTTGTAATCAATTGAGATTTCTTTAGT
GCTTGCTTTTCTGTGACTCAACTGCCCAGACACCTCATTGTACTTGAAAACTGGAACAGC
TTGGGAATGCCATGGGGTTTGATAATCTGCCAGGGACATGAAGAGGCTCAGCTTCCTGGA
CCATGACTTTGGCTCAGCTGATCCTGACATGGGAGAACAACCACATTTTTCTTTGTGTGT
GCTTCTAGCAGCTGTTCGGGAGGACCTTGACCCAATAGTGTTCCCATGCTGTTTCTTGTG
AAATGCTCTCGGCTATGTAGCAGCTTTTGATTCCCTGCATACCCTAGGCTGCTGCCCCTA
TCCTGTCCCTTGTTTATAACATTGAGAGGTTTTCTAGGGCACATACTGAGTGAGAGCAGT
GTTGAGAAGTCGGGGAAAATGGTGACTACTTTTAGAGCAAGGCTGGGCATCAGCACCTGT
CCAGCTCTACTTGTGTGATGTTTCAGGAACTCAGCCCCTTTTTCTGCCTAGGATAAGGAG
CTGAAAGATTAACTTGGATCTTCTAATGGTCCAAATCTTTTGGTCACAATAAAGAGTCTC
CAAATTAGAGACTGCATGTTAGTTCTGGATGGATTTGGTGGCCTGACATGATACCCTGCC
AGCTGTGAGGGGACCCCGTTTTTAAGATGCATGGCCAAGCTCTCTGCAAATGGAAATGCT
TACACTGGGTGTTGGGGATGTTTGCTACCTCCTGCTATTTTTGTGGTTTTGGTTCTCCCA
CTATGGTAGGACCCCTGGCCAGCATTGTGGCTTGTCATGTCAGCCCCATTGACTACCTTC
TCATGCTCTGAGGTACTACTGCCTCTGCAGCACAAATTTCTATTTCTGTCAATAAAAGGA
GATGAAAATATTCTAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MSNNGLDIQDKPPAPPMRNTSTMIGAGSKDAGTLNHGSKPLPPNPEEKKKKDRFYRSILP
GDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKN
PQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNALNVKAVSETPAVPPVSEDEDDD
DDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTE
KQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQ
MNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETC
MDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSK
RSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNG
TPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEA
TKNNH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: chloramphenicol

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLDNT7_nFLAG

Synonyms:

pLDNT7-Flag

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning

Comments:

None

Size (bp):

4557

Parent Vector:

None

Properties:

Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker

Author Name:

Daniel Schiwek

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGCCGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAGAGATCTATAACTTCGTATAGCATACATTATACGAAGTTATTGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTGGACCCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE1 ori

ColE1 origin of replication

1842

2524

promoter

T7 promoter

4462

4479

recombination site

LoxP

LoxP site for recombinational cloning

657

690

selectable marker

AmpR

ampicillin resistance gene

2622

3281

ssDNA origin

f1 ori

f1 origin for ssDNA production

3928

4368

tag

Flag

3xFlag epitope tag ORF and LoxP region

585

650

trxn termination sequence

T7 term

T7 terminator sequence

1614

1660

Gene

Gene Symbol :

PAK1

Symbol Nomenclature :

PAK1

SYNONYM :

PAKalpha

Designation :

Full Nomenclature :

p21 protein (Cdc42/Rac)-activated kinase 1

GENEID :

5058

HGNC :

8590

MIM :

602590

Vega :

OTTHUMG00000165138

Target GenBank:

NM_002576

Pathways

NCI :

Angiopoietin receptor Tie2-mediated signaling

NCI :

Aurora A signaling

NCI :

CDC42 signaling events

NCI :

CXCR4-mediated signaling events

NCI :

EGF receptor (ErbB1) signaling pathway

NCI :

EPHA2 forward signaling

NCI :

EPHB forward signaling

NCI :

Netrin-mediated signaling events

NCI :

PDGFR-beta signaling pathway

NCI :

PLK1 signaling events

NCI :

RAC1 signaling pathway

NCI :

Regulation of p38-alpha and p38-beta

NCI :

S1P2 pathway

NCI :

Signaling events mediated by focal adhesion kinase

NCI :

Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)

Panther :

Angiogenesis

Panther :

Axon guidance mediated by semaphorins

Panther :

Cytoskeletal regulation by Rho GTPase

Panther :

Inflammation mediated by chemokine and cytokine signaling pathway

Panther :

Ras Pathway

Panther :

T cell activation

Reactome :

Activation of Rac

Reactome :

Adaptive Immune System

Reactome :

Axon guidance

Reactome :

CD28 co-stimulation

Reactome :

CD28 dependent Vav1 pathway

Reactome :

Cell-Cell communication

Reactome :

Costimulation by the CD28 family

Reactome :

Developmental Biology

Reactome :

DSCAM interactions

Reactome :

Fc epsilon receptor (FCERI) signaling

Reactome :

FCERI mediated MAPK activation

Reactome :

Fcgamma receptor (FCGR) dependent phagocytosis

Reactome :

Generation of second messenger molecules

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

L1CAM interactions

Reactome :

Regulation of actin dynamics for phagocytic cup formation

Reactome :

Sema3A PAK dependent Axon repulsion

Reactome :

Semaphorin interactions

Reactome :

Signal transduction by L1

Reactome :

Signaling by Robo receptor

Reactome :

TCR signaling

WikiPathway :

angiogenesis overview

WikiPathway :

FAS pathway and Stress induction of HSP regulation

WikiPathway :

Focal Adhesion

WikiPathway :

Integrated Breast Cancer Pathway

WikiPathway :

Integrin-mediated cell adhesion

WikiPathway :

MAPK signaling pathway

WikiPathway :

Prolactin Signaling Pathway

WikiPathway :

Regulation of Actin Cytoskeleton

WikiPathway :

Signaling of Hepatocyte Growth Factor Receptor

WikiPathway :

TCR Signaling Pathway

Protein

SMART domain :

PBD : P21-Rho-binding domain

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

UniProt :

Q13153

HPRD :

03995

Clone

Cloning Information :

654624

HIP Master Clone ID :

136829

Original Clone ID :

HsCD00299289

Original Clone ID :

FLH231605.01L

Organism

Species Specific ID:

5058

Genome

Chromosome :

Map Location :

11q13-q14

Ensembl :

ENSG00000149269

Reagents

Labome :

PAK1-antibody

PAK1 (H. sapiens )In pLDNT7_nFLAG (Flag-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Organism

Genome

Reagents

PAK1 (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)