Gene:
Gene Symbol:
ABL1
Gene Name:
c-abl oncogene 1, non-receptor tyrosine kinase
Original Clone ID:
HsCD00299385
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617911
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3393nts
Open reading frame: 365 to 3757
GGCCTTCCCCCTGCGAGGATCGCCGTTGGCCCGGGTTGGCTTTGGAAAGCGGCGGTGGCT
TTGGGCCGGGCTCGGCCTCGGGAACGCCAGGGGCCCCTGGGTGCGGACGGGCGCGGCCAG
GAGGGGGTTAAGGCGCAGGCGGCGGCGGGGCGGGGGCGGGCCTGGCGGGCGCCCTCTCCG
GGCCCTTTGTTAACAGGCGCGTCCCGGCCAGCGGAGACGCGGCCGCCCTGGGCGGGCGCG
GGCGGCGGGCGGCGGTGAGGGCGGCCTGCGGGGCGGCGCCCGGGGGCCGGGCCGAGCCGG
GCCTGAGCCGGGCCCGGACCGAGCTGGGAGAGGGGCTCCGGCCCGATCGTTCGCTTGGCG
CAAAATGTTGGAGATCTGCCTGAAGCTGGTGGGCTGCAAATCCAAGAAGGGGCTGTCCTC
GTCCTCCAGCTGTTATCTGGAAGAAGCCCTTCAGCGGCCAGTAGCATCTGACTTTGAGCC
TCAGGGTCTGAGTGAAGCCGCTCGTTGGAACTCCAAGGAAAACCTTCTCGCTGGACCCAG
TGAAAATGACCCCAACCTTTTCGTTGCACTGTATGATTTTGTGGCCAGTGGAGATAACAC
TCTAAGCATAACTAAAGGTGAAAAGCTCCGGGTCTTAGGCTATAATCACAATGGGGAATG
GTGTGAAGCCCAAACCAAAAATGGCCAAGGCTGGGTCCCAAGCAACTACATCACGCCAGT
CAACAGTCTGGAGAAACACTCCTGGTACCATGGGCCTGTGTCCCGCAATGCCGCTGAGTA
TCCGCTGAGCAGCGGGATCAATGGCAGCTTCTTGGTGCGTGAGAGTGAGAGCAGTCCTAG
CCAGAGGTCCATCTCGCTGAGATACGAAGGGAGGGTGTACCATTACAGGATCAACACTGC
TTCTGATGGCAAGCTCTACGTCTCCTCCGAGAGCCGCTTCAACACCCTGGCCGAGTTGGT
TCATCATCATTCAACGGTGGCCGACGGGCTCATCACCACGCTCCATTATCCAGCCCCAAA
GCGCAACAAGCCCACTGTCTATGGTGTGTCCCCCAACTACGACAAGTGGGAGATGGAACG
CACGGACATCACCATGAAGCACAAGCTGGGCGGGGGCCAGTACGGGGAGGTGTACGAGGG
CGTGTGGAAGAAATACAGCCTGACGGTGGCCGTGAAGACCTTGAAGGAGGACACCATGGA
GGTGGAAGAGTTCTTGAAAGAAGCTGCAGTCATGAAAGAGATCAAACACCCTAACCTAGT
GCAGCTCCTTGGGGTCTGCACCCGGGAGCCCCCGTTCTATATCATCACTGAGTTCATGAC
CTACGGGAACCTCCTGGACTACCTGAGGGAGTGCAACCGGCAGGAGGTGAACGCCGTGGT
GCTGCTGTACATGGCCACTCAGATCTCGTCAGCCATGGAGTACCTAGAGAAGAAAAACTT
CATCCACAGAGATCTTGCTGCCCGAAACTGCCTGGTAGGGGAGAACCACTTGGTGAAGGT
AGCTGATTTTGGCCTGAGCAGGTTGATGACAGGGGACACCTACACAGCCCATGCTGGAGC
CAAGTTCCCCATCAAATGGACTGCACCCGAGAGCCTGGCCTACAACAAGTTCTCCATCAA
GTCCGACGTCTGGGCATTTGGAGTATTGCTTTGGGAAATTGCTACCTATGGCATGTCCCC
TTACCCGGGAATTGACCGTTCCCAGGTGTATGAGCTGCTAGAGAAGGACTACCGCATGAA
GCGCCCAGAAGGCTGCCCAGAGAAGGTCTATGAACTCATGCGAGCATGTTGGCAGTGGAA
TCCCTCTGACCGGCCCTCCTTTGCTGAAATCCACCAAGCCTTTGAAACAATGTTCCAGGA
ATCCAGTATCTCAGACGAAGTGGAAAAGGAGCTGGGGAAACAAGGCGTCCGTGGGGCTGT
GACTACCTTGCTGCAGGCCCCAGAGCTGCCCACCAAGACGAGGACCTCCAGGAGAGCTGC
AGAGCACAGAGACACCACTGACGTGCCTGAGATGCCTCACTCCAAGGGCCAGGGAGAGAG
CGATCCTCTGGACCATGAGCCTGCCGTGTCTCCATTGCTCCCTCGAAAAGAGCGAGGTCC
CCCGGAGGGCGGCCTGAATGAAGATGAGCGCCTTCTCCCCAAAGACAAAAAGACCAACTT
GTTCAGCGCCTTGATCAAGAAGAAGAAGAAGACAGCCCCAACCCCTCCCAAACGCAGCAG
CTCCTTCCGGGAGATGGACGGCCAGCCGGAGCGCAGAGGGGCCGGCGAGGAAGAGGGCCG
AGACATCAGCAACGGGGCACTGGCTTTCACCCCCTTGGACACAGCTGACCCAGCCAAGTC
CCCAAAGCCCAGCAATGGGGCTGGGGTCCCCAATGGAGCCCTCCGGGAGTCCGGGGGCTC
AGGCTTCCGGTCTCCCCACCTGTGGAAGAAGTCCAGCACGCTGACCAGCAGCCGCCTAGC
CACCGGCGAGGAGGAGGGCGGTGGCAGCTCCAGCAAGCGCTTCCTGCGCTCTTGCTCCGT
CTCCTGCGTTCCCCATGGGGCCAAGGACACGGAGTGGAGGTCAGTCACGCTGCCTCGGGA
CTTGCAGTCCACGGGAAGACAGTTTGACTCGTCCACATTTGGAGGGCACAAAAGTGAGAA
GCCGGCTCTGCCTCGGAAGAGGGCAGGGGAGAACAGGTCTGACCAGGTGACCCGAGGCAC
AGTAACGCCTCCCCCCAGGCTGGTGAAAAAGAATGAGGAAGCTGCTGATGAGGTCTTCAA
AGACATCATGGAGTCCAGCCCGGGCTCCAGCCCGCCCAACCTGACTCCAAAACCCCTCCG
GCGGCAGGTCACCGTGGCCCCTGCCTCGGGCCTCCCCCACAAGGAAGAAGCCTGGAAAGG
CAGTGCCTTAGGGACCCCTGCTGCAGCTGAGCCAGTGACCCCCACCAGCAAAGCAGGCTC
AGGTGCACCAAGGGGCACCAGCAAGGGCCCCGCCGAGGAGTCCAGAGTGAGGAGGCACAA
GCACTCCTCTGAGTCGCCAGGGAGGGACAAGGGGAAATTGTCCAAGCTCAAACCTGCCCC
GCCGCCCCCACCAGCAGCCTCTGCAGGGAAGGCTGGAGGAAAGCCCTCGCAGAGGCCCGG
CCAGGAGGCTGCCGGGGAGGCAGTCTTGGGCGCAAAGACAAAAGCCACGAGTCTGGTTGA
TGCTGTGAACAGTGACGCTGCCAAGCCCAGCCAGCCGGCAGAGGGCCTCAAAAAGCCCGT
GCTCCCGGCCACTCCAAAGCCACACCCCGCCAAGCCGTCGGGGACCCCCATCAGCCCAGC
CCCCGTTCCCCTTTCCACGTTGCCATCAGCATCCTCGGCCTTGGCAGGGGACCAGCCGTC
TTCCACTGCCTTCATCCCTCTCATATCAACCCGAGTGTCTCTTCGGAAAACCCGCCAGCC
TCCAGAGCGGGCCAGCGGCGCCATCACCAAGGGCGTGGTCTTGGACAGCACCGAGGCGCT
GTGCCTCGCCATCTCTGGGAACTCCGAGCAGATGGCCAGCCACAGCGCAGTGCTGGAGGC
CGGCAAAAACCTCTACACGTTCTGCGTGAGCTATGTGGATTCCATCCAGCAAATGAGGAA
CAAGTTTGCCTTCCGAGAGGCCATCAACAAACTGGAGAATAATCTCCGGGAGCTTCAGAT
CTGCCCGGCGTCAGCAGGCAGTGGTCCGGCGGCCACTCAGGACTTCAGCAAGCTCCTCAG
TTCGGTGAAGGAAATCAGTGACATAGTGCAGAGGTAGCAGCAGTCAGGGGTCAGGTGTCA
GGCCCGTCGGAGCTGCCTGCAGCACATGCGGGCTCGCCCATACCCATGACAGTGGCTGAG
AAGGGACTAGTGAGTCAGCACCTTGGCCCAGGAGCTCTGCGCCAGGCAGAGCTGAGGGCC
CTGTGGAGTCCAGCTCTACTACCTACGTTTGCACCGCCTGCCCTCCCGCACCTTCCTCCT
CCCCGCTCCGTCTCTGTCCTCGAATTTTATCTGTGGAGTTCCTGCTCCGTGGACTGCAGT
CGGCATGCCAGGACCCGCCAGCCCCGCTCCCACCTAGTGCCCCAGACTGAGCTCTCCAGG
CCAGGTGGGAACGGCTGATGTGGACTGTCTTTTTCATTTTTTTCTCTCTGGAGCCCCTCC
TCCCCCGGCTGGGCCTCCTTCTTCCACTTCTCCAAGAATGGAAGCCTGAACTGAGGCCTT
GTGTGTCAGGCCCTCTGCCTGCACTCCCTGGCCTTGCCCGTCGTGTGCTGAAGACATGTT
TCAAGAACCGCCATTTCGGGAAGGGCATGCACGGGCCATGCACACGGCTGGTCACTCTGC
CCTCTGCTGCTGCCCGGGGTGGGGTGCACTCGCCATTTCCTCACGTGCAGGACAGCTCTT
GATTTGGGTGGAAAACAGGGTGCTAAAGCCAACCAGCCTTTGGGTCCTGGGCAGGTGGGA
GCTGAAAAGGATCGAGGCATGGGGCATGTCCTTTCCATCTGTCCACATCCCCAGAGCCCA
GCTCTTGCTCTCTTGTGACGTGCACTGTGAATCCTGGCAAGAAAGCTTGAGTCTCAAGGG
TGGCAGGTCACTGTCACTGCCGACATCCCTCCCCCAGCAGAATGGAGGCAGGGGACAAGG
GAGGCAGTGGCTAGTGGGGTGAACAGCTGGTGCCAAATAGCCCCAGACTGGGCCCAGGCA
GGTCTGCAAGGGCCCAGAGTGAACCGTCCTTTCACACATCTGGGTGCCCTGAAGGGCCCT
TCCCCTCCCCCACTCCTCTAAGACAAAGTAGATTCTTACAAGGCCCTTTCCTTTGGAACA
AGACAGCCTTCACTTTTCTGAGTTCTTGAAGCATTTCAAAGCCCTGCCTCTGTGTAGCCG
CCCTGAGAGAGAATAGAGCTGCCACTGGGCACCTCGCGACAGGTGGGAGGAAAGGGCCTG
CGCAGTCCTGGTCCTGGCTGCACTCTTGAACTGGGCGAATGTCTTATTTAATTACCGTGA
GTGACATAGCCTCATGTTCTGTGGGGGTCATCAGGGAGGGTTAGGAAAACCACAAACGGA
GCCCCTGAAAGCCTCACGTATTTCACAGAGCACGCCTGCCATCTTCTCCCCGAGGCTGCC
CCAGGCCGGAGCCCAGATACCGGCGGGCTGTGACTCTGGGCAGGGACCCGGGGTCTCCTG
GACCTTGACAGAGCAGCTAACTCCGAGAGCAGTGGGCAGGTGGCCGCCCCTGAGGCTTCA
CGCCGGAGAAGCCACCTTCCCGCCCCTTCATACCGCCTCGTGCCAGCAGCCTCGCACAGG
CCCTAGCTTTACGCTCATCACCTAAACTTGTACTTTATTTTTCTGATAGAAATGGTTTCC
TCTGGATCGTTTTATGCGGTTCTTACAGCACATCACCTCTTTCCCCCCGACGGCTGTGAC
GCAGCGGAGAGGCACTAGTCACCGACAGCGGCCTTGAAGACAGAGCAAAGCCCCCACCCA
GGTCCCCCGACTGCCTGTCTCCATGAGGTACTGGTCCCTTCCTTTTGTTAACGTGATGTG
CCACTATATTTTACACGTATCTCTTGGTATGCATCTTTTATAGACGCTCTTTTCTAAGTG
GCGTGTGCATAGCGTCCTGCCCTGCCCTCGGGGGCCTGTGGTGGCTCCCCCTCTGCTTCT
CGGGGTCCAGTGCATTTTGTTTCTGTATATGATTCTCTGTGGTTTTTTTTGAATCCAAAT
CTGTCCTCTGTAGTATTTTTTAAATAAATCAGTGTTTACATTAG
Protein sequence predicted based on nucleotide sequence
MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSE NDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVN SLEKHSWYHGPVSRNAAEYPLSSGINGSFLVRESESSPSQRSISLRYEGRVYHYRINTAS DGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERT DITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQ LLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFI HRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKS DVWAFGVLLWEIATYGMSPYPGIDRSQVYELLEKDYRMKRPEGCPEKVYELMRACWQWNP SDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVTTLLQAPELPTKTRTSRRAAE HRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLF SALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSP KPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSVS CVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTV TPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAWKGS ALGTPAAAEPVTPTSKAGSGAPRGTSKGPAEESRVRRHKHSSESPGRDKGKLSKLKPAPP PPPAASAGKAGGKPSQRPGQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPAEGLKKPVL PATPKPHPAKPSGTPISPAPVPLSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPP ERASGAITKGVVLDSTEALCLAISGNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNK FAFREAINKLENNLRELQICPASAGSGPAATQDFSKLLSSVKEISDIVQR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
