Gene:
Gene Symbol:
PHKA2
Gene Name:
phosphorylase kinase, alpha 2 (liver)
Original Clone ID:
HsCD00299388
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617914
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GGCACGAGGCGCGCCTCCCCGCCGCGCGGAGCTCTGGTTGGCTTGCTTTCCAACCGGACT
TTGGGGCTAGCGTTTAGAAAAGTCTCGACCTCCTCCGGCCCGGTCCCATCCCAAGAACCG
ACTAAGGCTGTGAGTGTCCGGGAACCAGACCCGCTTGGAGGCCACAGCCCCGACGTCCCG
CGCCCACGCGGCAGATCGGGCGCTGCGGCCTGGGAGCCTCGGGGAGATGCGGAGCAGGAG
CAATTCCGGGGTCCGCTTGGACGGGTACGCGCGGCTGGTGCAGCAAACCATCCTGTGTTA
CCAGAATCCCGTCACGGGGCTGCTGTCAGCCAGCCATGAGCAGAAGGATGCCTGGGTGCG
GGATAACATCTACAGTATCCTGGCCGTGTGGGGCCTGGGCATGGCCTACCGTAAGAATGC
AGACCGCGATGAGGACAAGGCCAAGGCCTACGAGCTGGAGCAGAACGTGGTGAAGCTGAT
GCGAGGTCTTCTCCAGTGCATGATGAGACAGGTGGCCAAAGTGGAGAAGTTCAAACACAC
TCAGAGCACCAAGGACAGCCTGCACGCCAAGTACAACACCGCCACCTGTGGCACGGTGGT
GGGCGACGACCAGTGGGGCCACCTCCAGGTGGATGCCACCTCTCTCTTCCTCCTGTTCCT
GGCCCAGATGACCGCCTCAGGCTTACGTATCATTTTCACTCTCGATGAGGTGGCCTTCAT
ACAGAATCTTGTCTTTTACATAGAAGCTGCATATAAAGTCGCTGATTATGGAATGTGGGA
GCGTGGAGATAAGACTAATCAGGGCATCCCGGAATTGAATGCAAGCTCCGTAGGAATGGC
CAAGGCAGCTCTTGAGGCAATTGATGAACTGGACCTTTTTGGAGCCCATGGAGGACGCAA
GTCAGTGATTCATGTTCTGCCAGATGAGGTCGAGCACTGCCAGTCTATTCTGTTCTCCAT
GCTGCCAAGAGCGTCGACATCTAAAGAAATTGATGCTGGACTTCTTTCCATTATTTCCTT
CCCGGCCTTTGCAGTGGAAGATGTAAACCTTGTAAATGTGACCAAAAATGAAATTATTTC
TAAGCTCCAGGGGCGTTATGGATGCTGTCGCTTCCTTCGAGATGGTTATAAAACTCCAAG
AGAGGACCCTAATCGACTGCATTATGACCCTGCTGAACTCAAGCTCTTCGAAAACATTGA
ATGTGAGTGGCCTGTGTTTTGGACATATTTTATAATAGATGGAGTCTTCAGTGGTGATGC
TGTTCAGGTCCAAGAATACCGAGAGGCCCTGGAGGGAATACTCATCAGAGGCAAGAATGG
GATCCGCCTGGTGCCTGAACTCTACGCTGTCCCGCCTAACAAGGTAGATGAAGAGTACAA
GAATCCTCACACAGTAGACCGAGTTCCTATGGGGAAGGTGCCTCATCTGTGGGGCCAATC
CTTGTACATCCTCAGCTCGCTGTTGGCAGAGGGATTCCTTGCCGCTGGTGAAATCGATCC
CTTAAATAGAAGATTTTCCACTTCAGTCAAACCTGATGTTGTAGTACAAGTTACTGTTTT
GGCAGAAAACAATCACATTAAGGACTTATTGAGGAAACACGGGGTGAACGTCCAGAGTAT
CGCGGACATTCATCCAATTCAAGTCCAGCCGGGCCGGATTCTTAGTCACATATATGCCAA
GCTTGGACGGAATAAGAATATGAATTTGAGTGGGCGACCGTATCGACATATTGGTGTCCT
TGGAACCTCTAAACTATATGTGATTAGGAACCAAATCTTTACTTTTACACCCCAGTTCAC
CGACGAGCATCACTTCTACCTGGCCCTCGACAATGAGATGATCGTGGAGATGCTAAGGAT
CGAGCTGGCCTACCTGTGCACCTGCTGGAGGATGACGGGCAGACCCACACTCACCTTCCC
CATCAGTCGCACCATGCTCACAAATGATGGCTCAGACATTCATTCTGCTGTGCTCTCCAC
AATTAGAAAACTAGAGGATGGATATTTTGGAGGAGCCAGAGTAAAATTAGGGAACCTTTC
GGAATTTCTCACCACATCGTTCTACACATATCTGACTTTTCTGGATCCAGACTGTGATGA
GAAGTTGTTTGACAATGCCAGCGAAGGGACTTTCAGTCCTGATAGTGATTCAGATTTGGT
AGGATATCTGGAAGACACCTGTAATCAAGAAAGCCAAGACGAACTTGACCATTATATCAA
CCACCTTCTGCAAAGCACATCGTTGAGGTCCTATCTGCCTCCTCTTTGTAAGAACACAGA
AGACCGCCATGTCTTCAGTGCTATCCACTCCACGCGGGACATACTTTCTGTGATGGCAAA
AGCAAAGGGTTTGGAAGTTCCATTTGTTCCCATGACTTTGCCGACTAAAGTTCTAAGTGC
CCACCGTAAATCACTGAATCTTGTTGATTCTCCTCAGCCACTCCTAGAAAAGGTTCCTGA
AAGTGACTTTCAGTGGCCCAGAGATGACCATAGTGACGTGGACTGTGAGAAGCTGGTTGA
GCAGCTAAAAGATTGTTCGAACCTACAGGACCAAGCAGACATTCTGTACATTCTTTATGT
CATAAAGGGTCCCAGCTGGGACACAAATCTCTCTGGACAGCACGGGGTCACCGTTCAAAA
CCTTCTTGGTGAGCTCTATGGGAAAGCCGGCTTGAACCAGGAGTGGGGTCTGATTCGCTA
CATCTCAGGCCTTCTCAGGAAGAAAGTGGAGGTCCTGGCTGAGGCCTGCACAGACCTGCT
TTCGCACCAGAAGCAGCTCACCGTGGGCCTGCCGCCCGAGCCCCGGGAGAAGATCATCTC
TGCGCCCCTTCCCCCAGAGGAGCTCACAAAACTCATCTACGAGGCCAGTGGGCAGGACAT
CAGCATTGCCGTCCTCACGCAGGAGATTGTGGTTTACCTGGCCATGTATGTCAGGGCGCA
GCCCAGCCTCTTTGTGGAGATGCTGAGACTCCGGATTGGACTGATCATTCAGGTGATGGC
CACGGAGCTGGCACGGAGCCTGAACTGCTCAGGAGAAGAGGCTTCTGAAAGTTTGATGAA
CCTCAGCCCTTTCGATATGAAAAATCTCCTGCACCATATTCTAAGTGGGAAAGAGTTTGG
CGTTGAAAGAAGTGTGCGCCCTATCCACTCCTCCACATCCAGCCCTACCATCTCCATCCA
CGAGGTGGGCCATACCGGAGTCACCAAAACTGAGAGGAGTGGCATTAACAGACTGAGGAG
TGAAATGAAACAGATGACTAGGCGGTTTAGTGCTGATGAACAGTTCTTTTCTGTGGGCCA
GGCCGCGTCCAGCAGTGCGCATTCCTCCAAGTCTGCGAGGTCCAGCACCCCATCCTCGCC
CACTGGCACGTCATCCTCAGACTCGGGAGGACATCACATCGGCTGGGGTGAGCGGCAGGG
CCAGTGGCTGCGCAGGAGAAGGCTGGATGGGGCCATCAACAGGGTCCCCGTGGGATTCTA
CCAGAGGGTGTGGAAGATCCTCCAGAAGTGCCACGGTCTCTCCATCGATGGTTATGTCCT
CCCATCCTCGACGACCCGAGAGATGACCCCGCATGAGATCAAGTTTGCTGTCCATGTCGA
ATCGGTGCTGAACCGCGTGCCGCAGCCCGAGTACCGGCAGCTGCTGGTGGAAGCCATCAT
GGTGCTGACGCTGCTCTCGGACACGGAGATGACCAGCATCGGGGGCATCATCCACGTGGA
CCAGATCGTGCAGATGGCCAGTCAGCTGTTCTTGCAGGACCAGGTGTCAATTGGTGCCAT
GGACACCCTGGAGAAAGACCAAGCCACAGGAATCTGCCACTTCTTTTATGACAGCGCTCC
GAGTGGGGCTTATGGGACGATGACCTACCTAACAAGAGCAGTGGCTTCTTATTTGCAGGA
ATTGTTGCCCAATTCGGGCTGCCAGATGCAATAGGGTCTCACCTGGAAACATGATCACAC
TCTCAATCTGTCACGTGCCCCCTAGCCTTACTGGGAACCTTCTGTCCCCCAAGATCCCCT
GTGCTATCAGGAAAGCATGTCCCATCAGAAACACTCTCGGGGGGCAATGGTAGCACTCAC
CCTGAAACTGATGTATGTTAAAGCCACAGAGATAGAGCTGAGGAGTCCTGTGTTCCCCCG
CAAGGAGCACCCCGGGATCATTTTCTAGGTTCATTTCTCTGGAACATTTGCTGTAGCATC
TGGTCTCACGGACTCTGAGGAGGAATTGGAAATTGGTCTCTTTTGAGTGCAGAGGGAACT
GAGACGCCAGCTTAAATTGGCTCTTGCAGAGAGTTACAGAAATAGTTTCGATGAGCTAGT
GACACATCCTAAAGATGCAAAGATCCTCCTGGCGGCAGTAGCCTTGACAAGGGCCACCTC
TTCACAGGATGCAGTCTGTCTGTGCACCAAACTCTTCACCAAATAGAACACTTGTGTCTC
TCTGTGAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHEQKDAWVRDNIYSILAVWGLGMA YRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVAKVEKFKHTQSTKDSLHAKYNTAT CGTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVAD YGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQS ILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDG YKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFSGDAVQVQEYREALEGILI RGKNGIRLVPELYAVPPNKVDEEYKNPHTVDRVPMGKVPHLWGQSLYILSSLLAEGFLAA GEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKDLLRKHGVNVQSIADIHPIQVQPGRILS HIYAKLGRNKNMNLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDEHHFYLALDNEMIV EMLRIELAYLCTCWRMTGRPTLTFPISRTMLTNDGSDIHSAVLSTIRKLEDGYFGGARVK LGNLSEFLTTSFYTYLTFLDPDCDEKLFDNASEGTFSPDSDSDLVGYLEDTCNQESQDEL DHYINHLLQSTSLRSYLPPLCKNTEDRHVFSAIHSTRDILSVMAKAKGLEVPFVPMTLPT KVLSAHRKSLNLVDSPQPLLEKVPESDFQWPRDDHSDVDCEKLVEQLKDCSNLQDQADIL YILYVIKGPSWDTNLSGQHGVTVQNLLGELYGKAGLNQEWGLIRYISGLLRKKVEVLAEA CTDLLSHQKQLTVGLPPEPREKIISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAM YVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILS GKEFGVERSVRPIHSSTSSPTISIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQF FSVGQAASSSAHSSKSARSSTPSSPTGTSSSDSGGHHIGWGERQGQWLRRRRLDGAINRV PVGFYQRVWKILQKCHGLSIDGYVLPSSTTREMTPHEIKFAVHVESVLNRVPQPEYRQLL VEAIMVLTLLSDTEMTSIGGIIHVDQIVQMASQLFLQDQVSIGAMDTLEKDQATGICHFF YDSAPSGAYGTMTYLTRAVASYLQELLPNSGCQMQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
