DNASU Plasmid | CAMK2D (H. sapiens )<br>In pLDNT7_nFLAG (Flag-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: CAMK2D

Gene Name: calcium/calmodulin-dependent protein kinase II delta

Original Clone ID:

FLH231632.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLDNT7_nFLAG

Format:

CLOSED

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00617915

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1437nts

Open reading frame: 63 to 1499

GCCGCGCGTCTGCTCGCTTCTGGTCCGTCGCGCTCCCAGCCAGGGCACAGCCCGGACCGA
GGATGGCTTCGACCACAACCTGCACCAGGTTCACGGACGAGTATCAGCTTTTCGAGGAGC
TTGGAAAGGGGGCATTCTCAGTGGTGAGAAGATGTATGAAAATTCCTACTGGACAAGAAT
ATGCTGCCAAAATTATCAACACCAAAAAGCTTTCTGCTAGGGATCATCAGAAACTAGAAA
GAGAAGCTAGAATCTGCCGTCTTTTGAAGCACCCTAATATTGTGCGACTTCATGATAGCA
TATCAGAAGAGGGCTTTCACTACTTGGTGTTTGATTTAGTTACTGGAGGTGAACTGTTTG
AAGACATAGTGGCAAGAGAATACTACAGTGAAGCTGATGCCAGTCATTGTATACAGCAGA
TTCTAGAAAGTGTTAATCATTGTCACCTAAATGGCATAGTTCACAGGGACCTGAAGCCTG
AGAATTTGCTTTTAGCTAGCAAATCCAAGGGAGCAGCTGTGAAATTGGCAGACTTTGGCT
TAGCCATAGAAGTTCAAGGGGACCAGCAGGCGTGGTTTGGTTTTGCTGGCACACCTGGAT
ATCTTTCTCCAGAAGTTTTACGTAAAGATCCTTATGGAAAGCCAGTGGATATGTGGGCAT
GTGGTGTCATTCTCTATATTCTACTTGTGGGGTATCCACCCTTCTGGGATGAAGACCAAC
ACAGACTCTATCAGCAGATCAAGGCTGGAGCTTATGATTTTCCATCACCAGAATGGGACA
CGGTGACTCCTGAAGCCAAAGACCTCATCAATAAAATGCTTACTATCAACCCTGCCAAAC
GCATCACAGCCTCAGAGGCACTGAAGCACCCATGGATCTGTCAACGTTCTACTGTTGCTT
CCATGATGCACAGACAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGAAGAAAAC
TAAAGGGTGCCATCTTGACAACTATGCTGGCTACAAGGAATTTCTCAGCAGCCAAGAGTT
TGTTGAAGAAACCAGATGGAGTAAAGGAGTCAACTGAGAGTTCAAATACAACAATTGAGG
ATGAAGATGTGAAAGCACGAAAGCAAGAGATTATCAAAGTCACTGAACAACTGATCGAAG
CTATCAACAATGGGGACTTTGAAGCCTACACAAAAATCTGTGACCCAGGCCTTACTGCTT
TTGAACCTGAAGCTTTGGGTAATTTAGTGGAAGGGATGGATTTTCACCGATTCTACTTTG
AAAATGCTTTGTCCAAAAGCAATAAACCAATCCACACTATTATTCTAAACCCTCATGTAC
ATCTGGTAGGGGATGATGCCGCCTGCATAGCATATATTAGGCTCACACAGTACATGGATG
GCAGTGGAATGCCAAAGACAATGCAGTCAGAAGAGACTCGTGTGTGGCACCGCCGGGATG
GAAAGTGGCAGAATGTTCATTTTCATCGCTCGGGGTCACCAACAGTACCCATCAAGTAAA
TATTTCCAGGCTGTCAGCTTCTTTGTTAATACACCCATGGTCAGCTCCTTCTACTTATTC
CATTGTTAATAGCATGGTATATGTTATTTAACGCTAGTAGTTGGTTACACTGATGAAAAT
AAATGCCTTCACGGGAAAGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLER
EARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQI
LESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGY
LSPEVLRKDPYGKPVDMWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDT
VTPEAKDLINKMLTINPAKRITASEALKHPWICQRSTVASMMHRQETVDCLKKFNARRKL
KGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEA
INNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPIHTIILNPHVH
LVGDDAACIAYIRLTQYMDGSGMPKTMQSEETRVWHRRDGKWQNVHFHRSGSPTVPIK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: chloramphenicol

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLDNT7_nFLAG

Synonyms:

pLDNT7-Flag

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning

Comments:

None

Size (bp):

4557

Parent Vector:

None

Properties:

Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker

Author Name:

Daniel Schiwek

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGCCGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAGAGATCTATAACTTCGTATAGCATACATTATACGAAGTTATTGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTGGACCCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE1 ori

ColE1 origin of replication

1842

2524

promoter

T7 promoter

4462

4479

recombination site

LoxP

LoxP site for recombinational cloning

657

690

selectable marker

AmpR

ampicillin resistance gene

2622

3281

ssDNA origin

f1 ori

f1 origin for ssDNA production

3928

4368

tag

Flag

3xFlag epitope tag ORF and LoxP region

585

650

trxn termination sequence

T7 term

T7 terminator sequence

1614

1660

Gene

Gene Symbol :

CAMK2D

Symbol Nomenclature :

CAMK2D

SYNONYM :

CAMKD

Designation :

Full Nomenclature :

calcium/calmodulin-dependent protein kinase II delta

GENEID :

817

HGNC :

1462

MIM :

607708

Vega :

OTTHUMG00000132910

Target GenBank:

BC032784

Pathways

NCI :

IFN-gamma pathway

NCI :

Notch-mediated HES/HEY network

Panther :

Inflammation mediated by chemokine and cytokine signaling pathway

Panther :

Ionotropic glutamate receptor pathway

Reactome :

Activation of NMDA receptor upon glutamate binding and postsynaptic events

Reactome :

Cellular response to heat stress

Reactome :

Cellular responses to stress

Reactome :

CREB phosphorylation through the activation of CaMKII

Reactome :

CREB phosphorylation through the activation of Ras

Reactome :

Cytokine Signaling in Immune system

Reactome :

Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity

Reactome :

HSF1-dependent transactivation

Reactome :

Immune System

Reactome :

Interferon gamma signaling

Reactome :

Interferon Signaling

Reactome :

Neuronal System

Reactome :

Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell

Reactome :

Post NMDA receptor activation events

Reactome :

Ras activation uopn Ca2+ infux through NMDA receptor

Reactome :

Trafficking of AMPA receptors

Reactome :

Transmission across Chemical Synapses

Reactome :

Unblocking of NMDA receptor, glutamate binding and activation

WikiPathway :

Calcium Regulation in the Cardiac Cell

WikiPathway :

MicroRNAs in cardiomyocyte hypertrophy

WikiPathway :

Myometrial Relaxation and Contraction Pathways

WikiPathway :

Physiological and Pathological Hypertrophy of the Heart

WikiPathway :

SRF and miRs in Smooth Muscle Differentiation and Proliferation

Protein

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

UniProt :

Q13557

UniProt :

A8MVS8

HPRD :

09653

Clone

Cloning Information :

654651

HIP Master Clone ID :

134844

Original Clone ID :

HsCD00301001

Original Clone ID :

FLH231632.01L

Organism

Species Specific ID:

817

Genome

Chromosome :

Map Location :

4q26

Ensembl :

ENSG00000145349

Reagents

Labome :

CAMK2D-chemical

Labome :

CAMK2D-antibody

CAMK2D (H. sapiens )In pLDNT7_nFLAG (Flag-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Organism

Genome

Reagents

CAMK2D (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)