Gene:
Gene Symbol:
MAP4K3
Gene Name:
mitogen-activated protein kinase kinase kinase kinase 3
Original Clone ID:
HsCD00298615
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617969
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2685nts
Open reading frame: 92 to 2776
GAGCCGGCCGCGGCGCCCTCTCTCCGTGTGGCCCCCTGAGCGGCCCCCCTCCCCTGCCCG
GGAGGGAGGCGGGGGGCACCTGGGGCCCGCCATGAACCCCGGCTTCGATTTGTCCCGCCG
GAACCCGCAGGAGGACTTCGAGCTGATTCAGCGCATCGGCAGCGGCACCTACGGCGACGT
CTACAAGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAATAAAATTGGA
ACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATTATGATGAAAGACTGTAAACA
CCCAAATATTGTTGCTTATTTTGGAAGCTATCTCAGGCGAGATAAGCTTTGGATTTGCAT
GGAGTTTTGTGGAGGTGGTTCTTTACAGGATATTTATCACGTAACTGGACCTCTGTCAGA
ACTGCAAATTGCATATGTTAGCAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAA
AGGAAAAATGCACAGAGATATAAAGGGAGCTAACATTCTATTAACGGATAATGGTCATGT
GAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTACAATTGCCAAACGGAAGTC
TTTCATTGGCACACCATATTGGATGGCTCCAGAAGTTGCAGCTGTTGAGAGGAAGGGGGG
TTACAATCAACTCTGTGATCTCTGGGCAGTGGGAATCACTGCCATAGAACTTGCAGAGCT
TCAGCCTCCTATGTTTGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAA
TTTTCAGCCTCCTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCACTTTGT
GAAAATGGCACTTACCAAAAATCCGAAAAAAAGACCTACTGCTGAAAAATTATTACAGCA
TCCTTTTGTAACACAACATTTGACACGGTCTTTGGCAATCGAGCTGTTGGATAAAGTAAA
TAATCCAGATCATTCCACTTACCATGATTTCGATGATGATGATCCTGAGCCTCTTGTTGC
TGTACCACATAGAATTCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAACACGCTCAGA
GATAACCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCACATCA
TGAACTTCCCGACAGTGATGGTTTTTTGGACAGTTCAGAAGAAATATACTACACTGCAAG
ATCTAATCTGGATCTGCAACTGGAATATGGACAAGGACACCAAGGTGGTTACTTTTTAGG
TGCAAACAAGAGTCTTCTCAAGTCTGTTGAAGAAGAATTGCATCAGCGAGGACACGTCGC
ACATTTAGAAGATGATGAAGGAGATGATGATGAATCTAAACACTCAACTCTGAAAGCAAA
AATTCCACCTCCTTTGCCACCAAAGCCTAAGTCTATCTTCATACCACAGGAAATGCATTC
TACTGAGGATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTCAGGGAGCCCAGCAAA
GCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACACAAACCTGTTGC
CTTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTGAACGAGATGGCTCATTATGTCA
ACAACAGAATGAACATAGAGGCACAAACCTTTCAAGAAAAGAAAAGAAAGATGTACCAAA
GCCTATTAGTAATGGTCTTCCTCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAA
AGTTTTTAATGGGTGTCCCTTGAAAATTCACTGTGCATCATCATGGATAAACCCAGATAC
AAGAGATCAGTACTTGATATTTGGTGCCGAAGAAGGGATTTATACCCTCAATCTTAATGA
ACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAAGGTGTACATGGTTGTATGTAAT
GAACAATTGCTTGCTATCAATATCTGGTAAAGCTTCTCAGCTTTATTCCCATAATTTACC
AGGGCTTTTTGATTATGCAAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAA
ACTCCCTGACAGAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACCAA
ATGGTGCCAGAAGTGTTGTGTTGTAAGAAATCCTTACACGGGCCATAAATACCTATGTGG
AGCACTTCAGACTAGCATTGTTCTATTAGAATGGGTTGAACCAATGCAGAAATTTATGTT
AATTAAGCACATAGATTTTCCTATACCATGTCCACTTAGAATGTTTGAAATGCTGGTAGT
TCCTGAACAGGAGTACCCTTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGACTTCAACCA
AGTGGTTCGATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGAATCAGA
TACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATACCATCCTTGTATG
CTTGGACTGTTGTATAAAAATAGTAAATCTCCAAGGAAGATTAAAATCTAGCAGGAAATT
GTCATCAGAACTCACCTTTGATTTCCAGATTGAATCAATAGTGTGCCTACAAGACAGTGT
GCTAGCTTTCTGGAAACATGGAATGCAAGGTAGAAGTTTTAGATCTAATGAGGTAACACA
AGAAATTTCAGATAGCACAAGAATTTTCAGGCTGCTTGGATCTGACAGGGTCGTGGTTTT
GGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGTACATCCTGGCGGGTCA
TGAAAACAGTTACTGAGAATTGTTGTGCTTTGACAGTTAACTCTAGAAAGAAAGAACACT
ACCACTGCAACATTAATGGATGCTTGAAGCTGTACAAAAGCTGCAGTAACCTGTCTTCAG
TTACTTTGTAATTTATTGTGGCATGAGATAAGATGGGGAAAATTTTGTTTTAAGTGGTAT
GGATATATTTAGCATATTGAACCACACAAGTGCTTAATTCATTGTTATGTAATCTTTGTA
CATATAGGCAGTATTTTTTCTGTGAAACTTCATATTGCTGAAGACATACACTAAGAATTT
ATGTAGATAATGTACTTTTATGAGATGTACAAGTAAGTGTCTTATCTGTACAGATGTAAA
TGTTGATGAAAATGCAATTGGGGTTAATATTTTAAGAATTCTTTAGTATATTCTTGGGTG
TGGCTATATTACAAAATGGGATGCTGGCAATGAAACAATACATTTAACACTATTGTATTT
TTATTATATGTAATTTAGTAATATGAATATAAATCTTGTAACTTTTAAAATTGTAATGGA
GGCTGTAATCATTTTATAATCTTTTTAATTTTAATGCAAGTACACTGGTGTTTATATTTG
CACAAAGTATTGATATGTGATGTATTAAGTCACAAAAGTAAGCTGTGACATTGTCTATAA
GCATTTGGCTCCACAAATGTATTTGGATTGTTTTCTATGTGAAGCAAACCAATTATAATT
AACCACATGTTGTAGTAACTGGTCTTTTTATATTTAAGCAGAATCCTGTAAGATTGCTTG
TCTTTGCTTAAAAACAATACCTTTGAACATTTTTGAATCACAGAATAGCGGTACCATGAT
AGAATACTGCAATTGTGGTCAGAATTACAGTATGCACAAAGAATTAATTAGCATTATTAA
AGAGTCCTCACTAAACATTTCATATGATCACACTGAAGAACTGTAACATTCCATAGAGTG
AAGTGGTTCAAATTTCTCTTGGAATTTTTACTTTTGTTGGCCTTATTTTATGATCCTTTT
CATATTTCTTTTGACTTAGAGTATTAATACATGGCCAAAATAATTTAGTTACTACCTCAT
ACAAACAATATAATGGTTACTACACATCACAGGAACTTAGTTTTGGTTTAAGTCATTTTT
GATTGCTTTTTTCCAATGGAATATGTATATACCAGGTTTTAGCAAAATGCACACTTTTGG
CTCTTTTTGGTATATGTTCTTTATATTTTAATGTGAGTATATACACTAAGAACAAACTAA
ATTGTGATTTATGATCTTCATTTATTTTAATGATAATGGTTTTAAAATATGTTCCTGATT
GTACATATTGTAAAATAAACATGTTTTTTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAA
A
Protein sequence predicted based on nucleotide sequence
MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQ EIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRET LQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFIGTPYWMAP EVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKM KWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDF DDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLD SSEEIYYTARSNLDLQLEYGQGHQGGYFLGANKSLLKSVEEELHQRGHVAHLEDDEGDDD ESKHSTLKAKIPPPLPPKPKSIFIPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPP PRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTP KVHMGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHETSMEQLF PRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLPVAIPAHKLPDRILPRKF SVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQTSIVLLEWVEPMQKFMLIKHIDFPIPC PLRMFEMLVVPEQEYPLVCVGVSRGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVT QLERDTILVCLDCCIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQG RSFRSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENSY*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
