Gene:
Gene Symbol:
BMPR2
Gene Name:
bone morphogenetic protein receptor, type II (serine/threonine kinase)
Original Clone ID:
HsCD00301074
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617974
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3117nts
Open reading frame: 575 to 3691
CCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGAAGCACCGAAGCGAAACTTAAGG
AATCCTGCCTTCCCGGAGCCGCGGGCGATGCGACTAGGGCTGCCGGGCGCCGCCGCCGCC
CGTCCGGCTTCGTCCTTCCCGGCAGTCGGGAACTAGTTCTGACCCTCGCCCCCCGACCCC
GGATCGAATCCCCGCCCTCCGCACCCTGGATATGTTTTCTCCCAGACCTGGATATTTTTT
TGATATCGTGAAACTACGAGGGAAATAATTTGGGGGATTTCTTCTTGGCTCCCTGCTTTC
CCCACAGACATGCCTTCCGTTTGGAGGGCCGCGGCACCCCGTCCGAGGCGAAGGAACCCC
CCCAGCCGCGAGGGAGAGAAATGAAGGGAATTTCTGCAGCGGCATGAAAGCTCTGCAGCT
AGGTCCTCTCATCAGCCATTTGTCCTTTCAAACTGTATTGTGATACGGGCAGGATCAGTC
CACGGGAGAGAAGACGAGCCTCCCGGCTGTTTCTCCGCCGGTCTACTTCCCATATTTCTT
TTCTTTGCCCTCCTGATTCTTGGCTGGCCCAGGGATGACTTCCTCGCTGCAGCGGCCCTG
GCGGGTGCCCTGGCTACCATGGACCATCCTGCTGGTCAGCACTGCGGCTGCTTCGCAGAA
TCAAGAACGGCTATGTGCGTTTAAAGATCCGTATCAGCAAGACCTTGGGATAGGTGAGAG
TAGAATCTCTCATGAAAATGGGACAATATTATGCTCGAAAGGTAGCACCTGCTATGGCCT
TTGGGAGAAATCAAAAGGGGACATAAATCTTGTAAAACAAGGATGTTGGTCTCACATTGG
AGATCCCCAAGAGTGTCACTATGAAGAATGTGTAGTAACTACCACTCCTCCCTCAATTCA
GAATGGAACATACCGTTTCTGCTGTTGTAGCACAGATTTATGTAATGTCAACTTTACTGA
GAATTTTCCACCTCCTGACACAACACCACTCAGTCCACCTCATTCATTTAACCGAGATGA
GACAATAATCATTGCTTTGGCATCAGTCTCTGTATTAGCTGTTTTGATAGTTGCCTTATG
CTTTGGATACAGAATGTTGACAGGAGACCGTAAACAAGGTCTTCACAGTATGAACATGAT
GGAGGCAGCAGCATCCGAACCCTCTCTTGATCTAGATAATCTGAAACTGTTGGAGCTGAT
TGGCCGAGGTCGATATGGAGCAGTATATAAAGGCTCCTTGGATGAGCGTCCAGTTGCTGT
AAAAGTGTTTTCCTTTGCAAACCGTCAGAATTTTATCAACGAAAAGAACATTTACAGAGT
GCCTTTGATGGAACATGACAACATTGCCCGCTTTATAGTTGGAGATGAGAGAGTCACTGC
AGATGGACGCATGGAATATTTGCTTGTGATGGAGTACTATCCCAATGGATCTTTATGCAA
GTATTTAAGTCTCCACACAAGTGACTGGGTAAGCTCTTGCCGTCTTGCTCATTCTGTTAC
TAGAGGACTGGCTTATCTTCACACAGAATTACCACGAGGAGATCATTATAAACCTGCAAT
TTCCCATCGAGATTTAAACAGCAGAAATGTCCTAGTGAAAAATGATGGAACCTGTGTTAT
TAGTGACTTTGGACTGTCCATGAGGCTGACTGGAAATAGACTGGTGCGCCCAGGGGAGGA
AGATAATGCAGCCATAAGCGAGGTTGGCACTATCAGATATATGGCACCAGAAGTGCTAGA
AGGAGCTGTGAACTTGAGGGACTGTGAATCAGCTTTGAAACAAGTAGACATGTATGCTCT
TGGACTAATCTATTGGGAGATATTTATGAGATGTACAGACCTCTTCCCAGGGGAATCCGT
ACCAGAGTACCAGATGGCTTTTCAGACAGAGGTTGGAAACCATCCCACTTTTGAGGATAT
GCAGGTTCTCGTGTCTAGGGAAAAACAGAGACCCAAGTTCCCAGAAGCCTGGAAAGAAAA
TAGCCTGGCAGTGAGGTCACTCAAGGAGACAATCGAAGACTGTTGGGACCAGGATGCAGA
GGCTCGGCTTACTGCACAGTGTGCTGAGGAAAGGATGGCTGAACTTATGATGATTTGGGA
AAGAAACAAATCTGTGAGCCCAACAGTCAATCCAATGTCTACTGCTATGCAGAATGAACG
CAACCTGTCACATAATAGGCGTGTGCCAAAAATTGGTCCTTATCCAGATTATTCTTCCTC
CTCATACATTGAAGACTCTATCCATCATACTGACAGCATCGTGAAGAATATTTCCTCTGA
GCATTCTATGTCCAGCACACCTTTGACTATAGGGGAAAAAAACCGAAATTCAATTAACTA
TGAACGACAGCAAGCACAAGCTCGAATCCCCAGCCCTGAAACAAGTGTCACCAGCCTCTC
CACCAACACAACAACCACAAACACCACAGGACTCACGCCAAGTACTGGCATGACTACTAT
ATCTGAGATGCCATACCCAGATGAAACAAATCTGCATACCACAAATGTTGCACAGTCAAT
TGGGCCAACCCCTGTCTGCTTACAGCTGACAGAAGAAGACTTGGAAACCAACAAGCTAGA
CCCAAAAGAAGTTGATAAGAACCTCAAGGAAAGCTCTGATGAGAATCTCATGGAGCACTC
TCTTAAACAGTTCAGTGGCCCAGACCCACTGAGCAGTACTAGTTCTAGCTTGCTTTACCC
ACTCATAAAACTTGCAGTAGAAGCAACTGGACAGCAGGACTTCACACAGACTGCAAATGG
CCAAGCATGTTTGATTCCTGATGTTCTGCCTACTCAGATCTATCCTCTCCCCAAGCAGCA
GAACCTTCCCAAGAGACCTACTAGTTTGCCTTTGAACACCAAAAATTCAACAAAAGAGCC
CCGGCTAAAATTTGGCAGCAAGCACAAATCAAACTTGAAACAAGTCGAAACTGGAGTTGC
CAAGATGAATACAATCAATGCAGCAGAACCTCATGTGGTGACAGTCACCATGAATGGTGT
GGCAGGTAGAAACCACAGTGTTAACTCCCATGCTGCCACAACCCAATATGCCAATGGGAC
AGTACTATCTGGCCAAACAACCAACATAGTGACACATAGGGCCCAAGAAATGTTGCAGAA
TCAGTTTATTGGTGAGGACACCCGGCTGAATATTAATTCCAGTCCTGATGAGCATGAGCC
TTTACTGAGACGAGAGCAACAAGCTGGCCATGATGAAGGTGTTCTGGATCGTCTTGTGGA
CAGGAGGGAACGGCCACTAGAAGGTGGCCGAACTAATTCCAATAACAACAACAGCAATCC
ATGTTCAGAACAAGATGTTCTTGCACAGGGTGTTCCAAGCACAGCAGCAGATCCTGGGCC
ATCAAAGCCCAGAAGAGCACAGAGGCCTAATTCTCTGGATCTTTCAGCCACAAATGTCCT
GGATGGCAGCAGTATACAGATAGGTGAGTCAACACAAGATGGCAAATCAGGATCAGGTGA
AAAGATCAAGAAACGTGTGAAAACTCCCTATTCTCTTAAGCGGTGGCGCCCCTCCACCTG
GGTCATCTCCACTGAATCGCTGGACTGTGAAGTCAACAATAATGGCAGTAACAGGGCAGT
TCATTCCAAATCCAGCACTGCTGTTTACCTTGCAGAAGGAGGCACTGCTACAACCATGGT
GTCTAAAGATATAGGAATGAACTGTCTGTGAAATGTTTTCAAGCCTATGGAGTGAAATTA
TTTTTTGCATCATTTAAACATGCAGAAGATGTTTAAAAATAAAAAAAAAACTGCTTTATC
CTCCTGTCAGCACCCCCTCCCACCCCTGCAACAAAGACTTGCTTTAAATAGATTTCAGCT
ATGCAGAAAAATTTAGCTTATGCTTCCATATTTTTAAATTTTGTTTTTTAAGTTTTGCAC
TTTTGTTTAGTCTCGCTAAAGTTATATTTGTCTGTTATGACCACAGAGTTATATGTGTGT
GTATCAAAAGTGGTCTCAAAATATTTTTTTAAGAAAAAAGCAAAAACAATGTATTGCTGA
TAATCAGTTTGGACCAGTTTCTTAAGGTCATTAAAACAGAAGCAAATTAAGACAGGTTTG
ACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MTSSLQRPWRVPWLPWTILLVSTAAASQNQERLCAFKDPYQQDLGIGESRISHENGTILC SKGSTCYGLWEKSKGDINLVKQGCWSHIGDPQECHYEECVVTTTPPSIQNGTYRFCCCST DLCNVNFTENFPPPDTTPLSPPHSFNRDETIIIALASVSVLAVLIVALCFGYRMLTGDRK QGLHSMNMMEAAASEPSLDLDNLKLLELIGRGRYGAVYKGSLDERPVAVKVFSFANRQNF INEKNIYRVPLMEHDNIARFIVGDERVTADGRMEYLLVMEYYPNGSLCKYLSLHTSDWVS SCRLAHSVTRGLAYLHTELPRGDHYKPAISHRDLNSRNVLVKNDGTCVISDFGLSMRLTG NRLVRPGEEDNAAISEVGTIRYMAPEVLEGAVNLRDCESALKQVDMYALGLIYWEIFMRC TDLFPGESVPEYQMAFQTEVGNHPTFEDMQVLVSREKQRPKFPEAWKENSLAVRSLKETI EDCWDQDAEARLTAQCAEERMAELMMIWERNKSVSPTVNPMSTAMQNERNLSHNRRVPKI GPYPDYSSSSYIEDSIHHTDSIVKNISSEHSMSSTPLTIGEKNRNSINYERQQAQARIPS PETSVTSLSTNTTTTNTTGLTPSTGMTTISEMPYPDETNLHTTNVAQSIGPTPVCLQLTE EDLETNKLDPKEVDKNLKESSDENLMEHSLKQFSGPDPLSSTSSSLLYPLIKLAVEATGQ QDFTQTANGQACLIPDVLPTQIYPLPKQQNLPKRPTSLPLNTKNSTKEPRLKFGSKHKSN LKQVETGVAKMNTINAAEPHVVTVTMNGVAGRNHSVNSHAATTQYANGTVLSGQTTNIVT HRAQEMLQNQFIGEDTRLNINSSPDEHEPLLRREQQAGHDEGVLDRLVDRRERPLEGGRT NSNNNNSNPCSEQDVLAQGVPSTAADPGPSKPRRAQRPNSLDLSATNVLDGSSIQIGEST QDGKSGSGEKIKKRVKTPYSLKRWRPSTWVISTESLDCEVNNNGSNRAVHSKSSTAVYLA EGGTATTMVSKDIGMNCL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
