Gene:
Gene Symbol:
DGKQ
Gene Name:
diacylglycerol kinase, theta 110kDa
Original Clone ID:
FLH231788.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617978
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2829nts
Open reading frame: 87 to 2915
CCCACGCGTCCGGTACCGGGCAGTGTAGCGGCGGCTCCTGTACCCCCGGCGCGGACCTAA
AGGGGCTCGGGCCGCTCGGGCCGGGAATGGCGGCGGCGGCCGAGCCCGGGGCCCGCGCCT
GGCTGGGCGGCGGCTCCCCGCGCCCCGGCAGCCCGGCCTGCAGCCTCGTGCTGGGCTCAG
GAGGCCGCGCGCGCCCGGGGCCGGGGCCGGGGCCGGGACCCGAGCGGGCGGGCGTCAGAG
CCCCGGGCCCCGCTGCCGCGCCGGGACACAGCTTCCGGAAGGTGACGCTCACCAAGCCCA
CCTTCTGCCACCTCTGCTCCGACTTCATCTGGGGGCTGGCCGGCTTCCTGTGCGACGTCT
GCAATTTCATGTCTCATGAGAAGTGCCTGAAGCACGTGAGGATCCCGTGCACGAGTGTGG
CACCCAGCCTGGTCCGGGTTCCTGTAGCCCACTGCTTCGGCCCCCGGGGGCTCCACAAGC
GCAAGTTCTGTGCTGTCTGCCGCAAGGTCCTGGAGGCACCGGCGCTCCACTGCGAAGTGT
GTGAGCTGCACCTCCACCCAGACTGTGTGCCCTTCGCCTGCAGTGACTGCCGCCAGTGCC
ACCAGGATGGGCACCAGGATCACGACACCCATCACCACCACTGGCGGGAGGGGAACCTGC
CCTCGGGAGCGCGCTGCGAGGTCTGCAGGAAGACGTGCGGCTCCTCTGACGTGCTGGCCG
GCGTGCGCTGCGAGTGGTGCGGGGTCCAGGCGCACTCCCTCTGCTCCGCGGCACTGGCTC
CCGAGTGTGGCTTCGGGCGTCTGCGCTCCCTGGTCCTGCCTCCCGCGTGCGTGCGCCTTC
TGCCCGGCGGCTTCAGCAAGACGCAGAGCTTCCGCATCGTGGAGGCCGCGGAGCCGGGCG
AGGGGGGCGACGGCGCCGACGGGAGCGCTGCCGTGGGTCCAGGCAGAGAGACACAGGCAA
CTCCGGAGTCCGGGAAGCAAACGCTGAAGATCTTTGATGGCGACGACGCGGTGAGAAGAA
GCCAGTTCCGCCTCGTCACGGTGTCCCGCCTGGCCGGTGCCGAGGAGGTGCTGGAGGCCG
CACTGCGGGCCCACCACATCCCCGAGGACCCTGGCCACCTGGAGCTGTGCCGGCTGCCCC
CTTCCTCTCAGGCCTGTGACGCCTGGGCTGGGGGCAAGGCTGGGAGTGCTGTGATCTCGG
AGGAGGGCAGAAGCCCCGGGTCCGGCGAGGCCACGCCAGAGGCCTGGGTCATCCGGGCTC
TGCCGCGGGCCCAGGAGGTCCTGAAGATCTACCCTGGCTGGCTCAAGGTGGGCGTGGCCT
ACGTGTCCGTGCGAGTGACCCCGAAGAGCACGGCCCGCTCTGTGGTGCTGGAGGTCCTGC
CGCTGCTCGGCCGCCAGGCCGAGAGTCCCGAGAGCTTCCAGCTGGTGGAGGTGGCGATGG
GCTGCAGGCACGTCCAGCGGACGATGCTGATGGACGAACAGCCCCTGCTGGACCGGCTAC
AGGACATCCGGCAGATGTCTGTGCGGCAGGTGAGCCAGACGCGGTTCTACGTGGCAGAGA
GCAGGGATGTAGCCCCGCACGTCTCCCTGTTTGTTGGCGGCCTGCCTCCCGGCCTGTCTC
CCGAGGAGTACAGCAGCCTGCTGCATGAGGCCGGGGCTACCAAAGCCACCGTGGTGTCCG
TGAGTCACATCTACTCCTCCCAAGGCGCGGTAGTGTTGGACGTTGCCTGCTTTGCGGAGG
CCGAGCGGCTGTACATGCTGCTGAAGGACATGGCTGTGCGGGGCCGGCTGCTCACTGCCC
TGGTGCTCCCCGACCTGCTGCACGCGAAGCTGCCCCCAGACAGCTGTCCCCTCCTTGTGT
TCGTGAACCCCAAGAGTGGAGGCCTCAAGGGCCGAGACCTGCTCTGCAGCTTCCGGAAGC
TACTGAACCCTCATCAGGTCTTCGACCTGACCAACGGAGGTCCTCTTCCCGGGCTCCACC
TGTTCTCCCAGGTGCCCTGCTTCCGGGTGCTGGTGTGTGGTGGCGATGGCACTGTGGGCT
GGGTGCTTGGCGCCCTGGAGGAGACACGGTACCGACTGGCCTGCCCGGAGCCTTCTGTGG
CCATCCTGCCCCTGGGCACAGGGAATGACCTTGGTCGAGTCCTCCGCTGGGGGGCGGGCT
ACAGCGGCGAGGACCCGTTCTCCGTACTGCTGTCTGTGGACGAGGCCGACGCCGTGCTCA
TGGACCGCTGGACCATCCTGCTGGATGCCCACGAGGCTGGCAGTGCAGAGAACGACACGG
CAGACGCAGAGCCCCCCAAGATCGTGCAGATGAGTAACTACTGTGGCATTGGCATCGACG
CGGAGCTGAGCCTGGACTTCCACCAGGCACGGGAAGAGGAGCCTGGCAAGTTCACAAGCA
GGCTGCACAACAAGGGTGTGTACGTGCGGGTGGGGCTGCAGAAGATCAGTCACTCTCGGA
GCCTGCACAAGCAGATCCGGCTGCAGGTGGAGCGGCAGGAGGTGGAGCTGCCCAGTATTG
AAGGCCTCATCTTCATCAACATCCCCAGCTGGGGCTCGGGGGCCGACCTGTGGGGCTCCG
ACAGCGACACCAGGTTTGAGAAGCCACGCATGGACGACGGGCTGCTGGAGGTTGTGGGCG
TGACGGGCGTCGTGCACATGGGCCAGGTCCAGGGTGGGCTGCGCTCCGGAATCCGGATTG
CCCAGGGTTCCTACTTCCGAGTCACGCTCCTCAAGGCCACCCCGGTGCAGGTGGACGGGG
AGCCCTGGGTCCAGGCCCCGGGGCACATGATCATCTCAGCTGCTGGCCCTAAGGTGCACA
TGCTGAGGAAGGCCAAGCAGAAGCCGAGGAGGGCCGGGACCACCAGGGATGCCCGGGCGG
ATGCTGCGCCTGCCCCTGAGAGCGATCCTAGGTAGGGGTGGCTGGGGCAGCCCAAGGGCT
CGAGCCATCTCTGCTCCCGCCAGCCTTGTTTTCAGGTGGTCTGGAGGCAGCTCCACGTCC
ACACAGTGGCCGGCCCTGTGGTCGGACCTGGCTACAGCCCTGACCGCAGCATCTTCCCAG
TGACGGAGCGGGACCCGCCCTCATCCCATTGGTGACACATGTTGGTGTCACAGCCCTCAT
CCCACTGGAGACTGGTGTGCGGGTGGCAGTGTTACCCCGTGTCCCGGGTGGGCGCAGGGC
TCTGGATGGTGAGCCCTCTGCCCTGTGGGGGCCTGGTCCTCTCCTCAGGGGTGGGCACCC
ATCCCTCTCCCCACTGAGCCCCTTGAGCTGGACAGTGCTGGATGCAGGCCGTCCAGGTGG
GTCTGATCTCACTTTGTGCCCCTCGGGCCGTCCCCCTGGCCAGTCCTTCAGAGCAGCAGG
CTCCCTGGTGTGTCCCTGCCCCAGCAGACACAGGCTGCCAGTCCCTCCAGCCTCACCTCC
CCGCCCTGGGCAGCAGGTGTGGGCAGCGTGTGAGGCTGCCGGGTGGTGGTGACTGGTGAC
TTCCTTGTGTTCAGGGAAGGGAGAAGGTGCCTTCCCTTGTTTTCTGGCCTTGTTATATAC
AGATGGCAGCTTGGATCTCAGGTACAGCTCCAGGGGCAGGCAGTGCCCAGCTGGACCTGG
TGGCCCTTTCCTAGTGCCTCTGCTTGGGGAGGAGAACCTCTGTCCACGTGGAGGCTAGGA
GGTCTCAGGTGCTGCCCTGGCAGCACCAGAGTGTGGGCCGGGCCCGAGTGTCTGCCCCTC
GGCCCTCAGGGTGGGGCACTTAGCACCCAGAAGGGACCAAAAGCAGGGCATGGCGGTGCA
GAGGAGTTTGGGAGGTGTAAACAGCCCCATGCACGTGGAGGAGGAGCTGGCTTTCAGCCC
CAGACCCCACGCTAGCACTTTCCACGCTGCTTGCCCGCTGTTGATGTGCAGTTCCCAGTG
CCTGTGTGAGCCGACATCTGCTCAGTCCTATCCCTCGTCAGCGTGTGGAGACCCAGCTCC
TGCAGCCCTCCTGCTCCCACGCCCCCAGACAGCTTGGTGGAGGGTCCTGCATCTGGGCCA
GGCTGGGGTGCACCCAGCCAAAGACAAAGCTGCCTCCACGTGCCCAAGGATTCAGATGGT
GCACTGGCCCCGGGAGGAGTCTGACCAAAAATGGAGCCCGCTCTGTGGGGAAGCCCCGAC
TCCCCCACGAGAAACGGTCCCACGGTGCGGATCTCCCCCTTCCCTTGTGGGGCACAGCTG
GCCTGGGCCTCCAATCCTGCGGAGCTTTCCTGGGTGTGGCTTTGACCTCAGAAGTGGCTC
TGGTTTGGCCTCAGGAGTGTGGCCTGGCCCAGCCTGCTGCAGCCTCCTGGGGGGCCCTTG
ATGCCACTAATCCCCCGACCCCCCGCATCTGCCAAACTGCACAGACACACGCATTGTAAG
GCCGCTTGTGGCCTCCAGCGTGCACTCTTGTTTACGTCATTGTCATCTTCAAGACCAGTC
CTTTGTGATTAGTTTTGCTTCGCGAGCCCTGGTGTGGACTGTGGTCTGTATGAATCGTGT
GTAACTGTGGTGAGGGGCTTGTCCTGTATGTGAGTCTGTACCCAGGTGGGGTCTGTGCCC
TGCACACCGGGCCCCTCTGTATTTATCGCTGCCTGAATGCAACAGTAATTTATATCCAGG
ACAAATACAGTCTGGGCGTCACTATCCTAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MAAAAEPGARAWLGGGSPRPGSPACSLVLGSGGRARPGPGPGPGPERAGVRAPGPAAAPG HSFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKHVRIPCTSVAPSLVRVPV AHCFGPRGLHKRKFCAVCRKVLEAPALHCEVCELHLHPDCVPFACSDCRQCHQDGHQDHD THHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSLCSAALAPECGFGRLR SLVLPPACVRLLPGGFSKTQSFRIVEAAEPGEGGDGADGSAAVGPGRETQATPESGKQTL KIFDGDDAVRRSQFRLVTVSRLAGAEEVLEAALRAHHIPEDPGHLELCRLPPSSQACDAW AGGKAGSAVISEEGRSPGSGEATPEAWVIRALPRAQEVLKIYPGWLKVGVAYVSVRVTPK STARSVVLEVLPLLGRQAESPESFQLVEVAMGCRHVQRTMLMDEQPLLDRLQDIRQMSVR QVSQTRFYVAESRDVAPHVSLFVGGLPPGLSPEEYSSLLHEAGATKATVVSVSHIYSSQG AVVLDVACFAEAERLYMLLKDMAVRGRLLTALVLPDLLHAKLPPDSCPLLVFVNPKSGGL KGRDLLCSFRKLLNPHQVFDLTNGGPLPGLHLFSQVPCFRVLVCGGDGTVGWVLGALEET RYRLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWTILLD AHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRLHNKGVYV RVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIFINIPSWGSGADLWGSDSDTRFEKP RMDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGH MIISAAGPKVHMLRKAKQKPRRAGTTRDARADAAPAPESDPR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
