Gene:
Gene Symbol:
MKNK1
Gene Name:
MAP kinase interacting serine/threonine kinase 1
Original Clone ID:
HsCD00301077
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617987
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1398nts
Open reading frame: 174 to 1571
GGCACGAGGGCGACCGCTCCCCGGCGGGAGCCAGCGAAGGTTTCCATGTCAGAGGCCGAT
GGAGAACTGAAGATTGCCACCTACGCACAAAGGCCATTGAGACACTTCGTGTAGCTGGAA
GACACCAACTTCCTGACAGGAGCTTTATTTCATTTGGGATTTCAAGTTTACAGATGGTAT
CTTCTCAAAAGTTGGAAAAACCTATAGAGATGGGCAGTAGCGAACCCCTTCCCATCGCAG
ATGGTGACAGGAGGAGGAAGAAGAAGCGGAGGGGCCGGGCCACTGACTCCTTGCCAGGAA
AGTTTGAAGATATGTACAAGCTGACCTCTGAATTGCTTGGAGAGGGAGCCTATGCCAAAG
TTCAAGGTGCCGTGAGCCTACAGAATGGCAAAGAGTATGCCGTCAAAATCATCGAGAAAC
AAGCAGGGCACAGTCGGAGTAGGGTGTTTCGAGAGGTGGAGACGCTGTATCAGTGTCAGG
GAAACAAGAACATTTTGGAGCTGATTGAGTTCTTTGAAGATGACACAAGGTTTTACTTGG
TCTTTGAGAAATTGCAAGGAGGTTCCATCTTAGCCCACATCCAGAAGCAAAAGCACTTCA
ATGAGCGAGAAGCCAGCCGAGTGGTGCGGGACGTTGCTGCTGCCCTTGACTTCCTGCATA
CCAAAGACAAAGTCTCTCTCTGTCACCTAGGCTGGAGTGCTATGGCGCCATCAGGGCTCA
CTGCAGCCCCAACCTCCCTGGGCTCCAGTGATCCTCCCACCTCAGCCTCCCAAGTAGCTG
GGACTACAGGCATTGCTCATCGTGATCTGAAACCAGAAAATATATTGTGTGAATCTCCAG
AAAAGGTGTCTCCAGTGAAAATCTGTGACTTTGACTTGGGCAGTGGGATGAAACTGAACA
ACTCCTGTACCCCCATAACCACACCAGAGCTGACCACCCCATGTGGCTCTGCAGAATACA
TGGCCCCTGAGGTAGTGGAGGTCTTCACGGACCAGGCCACATTCTACGACAAGCGCTGTG
ACCTGTGGAGCCTGGGCGTGGTCCTCTACATCATGCTGAGTGGCTACCCACCCTTCGTGG
GTCACTGCGGGGCCGACTGTGGCTGGGACCGGGGCGAGGTCTGCAGGGTGTGCCAGAACA
AGCTGTTTGAAAGCATCCAGGAAGGCAAGTATGAGTTTCCTGACAAGGACTGGGCACACA
TCTCCAGTGAAGCCAAAGACCTCATCTCCAAGCTCCTGGTGCGAGATGCAAAGCAGAGAC
TTAGCGCCGCCCAAGTTCTGCAGCACCCATGGGTGCAGGGGCAAGCTCCAGAAAAGGGAC
TCCCCACGCCGCAAGTCCTCCAGAGGAACAGCAGCACAATGGACCTGACGCTCTTCGCAG
CTGAGGCCATCGCCCTTAACCGCCAGCTATCTCAGCACGAAGAGAACGAACTAGCAGAGG
AGCCAGAGGCACTAGCTGATGGCCTCTGCTCCATGAAGCTTTCCCCTCCCTGCAAGTCAC
GCCTGGCCCGGAGACGGGCCCTGGCCCAGGCAGGCCGTGGTGAAGACAGGAGCCCGCCCA
CAGCACTCTGAAATGCTCCAGTCACACCTTATAGGCCCTAGGCCTGGCCAGGCATTGTCC
CCTGGAAACCTGTGTGGCTAAAGTCTGCTGAGCAGGCAGCAGCCTCTGCTCTGTGGCTCC
ATTCAGGCTTTTTCATCTACGAAGGCCCTGAGGTTCCCATCAACCCCCATTTCCCTAGGG
TCCTGGAGGAAAAAGCTTTTTCCAAAGGGGTTGTCTTTGAAAAGGAAAGCAATCACTTCT
CACTTTGCATAATTGCCTGCAGCAGGAACATCTCTTCACTGGGCTCCACCTGCTCACCCG
CCTGCAGATCTGGGATCCAGCCTGCTCTCACCGCTGTAGCTGTGGCGGCTGGGGCTGCAG
CCTGCAGGGAGAAGCAAGAAGCATCAGTTGACAGAGGCTGCCGACACGTGCCTCTTCCCT
CTCTTCTCTGTCACCCTCCTCTGGCGGTCCTTCCACCTTCCTCTGTCCTCCGGATGTCCT
CTTTGCCCGTCTTCTCCCTTGGCTGAGCAAAGCCATCCCCTCAATTCAGGGAAGGGCAAG
GAGCCTTCCTCATTCAGGAAATCAAATCAGTCTTCCGGTCTGCAGCACGGAAAAGCACAT
AATCTTTCTTTGCTGTGACTGAAATGTATCCCTCGTTTATCATCCCCTTTGTTTGTGATT
GCTGCTAAAGTCAGTAGTATCGTTTTTTTAAAAAAAAAGTTTGGTGTTTTTAACCATGCT
GTTCCAGCAAAGATGATACCTTAAACTCCCACTGCAAGCCCATGAACTTCCCAGAGAGTG
GAACGGCTTGCTCTTCTTTCTAGAATGTCCATGCACTTGGGTTTTAATCAGCAGTTCCCT
ATTATTCTGATTTTAAGCTGTTCCTGTGATGAACTTAGAGACAGCATCGGTGTCTGCTGC
TGTGTCCCCAGGTCTTGTGTGGGTGGCACAGATCTGGGCAGTTAGATAGTGCTCTGTGCC
TAAGGTGAAGCCACACTAGGGTGAAGCCTCACTTCCCTGTTTGAGCAATGCAGTGCCTGC
TGCCCGTGTGCATGAAGGTACAGCCATTCAGATAAGTGGAACTATTGAGTTACATAAAGA
AAATAGATTTGCATTTGTCAGGCAGACGTTTATACAACACCACGGTGCTTTTATACATTG
TGCTTATTTTAATAAAACTGAAATTCTAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAY AKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRF YLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPS GLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMK LNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPP FVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAK QRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENEL AEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
