Gene:
Gene Symbol:
CAMK2G
Gene Name:
calcium/calmodulin-dependent protein kinase II gamma
Original Clone ID:
HsCD00298623
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618024
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1584nts
Open reading frame: 122 to 1705
GGAGTCTCGCGGTGCTGCCGGGCTCAGCCCCGTCTCCTCCTCTTGCTCCCTCGGCCGGGC
GGCGGTGACTGTGCACCGACGTCGGCGCGGGCTGCACCGCCGCGTCCACCCGCCCGCCAG
CATGGCCACCACCGCCACCTGCACACGTTTCACCGACGACTACCAGCTCTTCGAGGAGCT
TGGCAAGGGTGCTTTCTCTGTGGTCCGCAGGTGTGTGAAGAAAACCCCCACGCAGGAGTA
CGCAGCAAAAATCATCAATACCAAGAAATTGTCTGCCCGGGATCACCAGAAACTAGAACG
TGAGGCTCGGATATGTCGACTTCTGAAACATCCAAACATCGTGCGCCTCCATGACAGTAT
TTCTGAAGAAGGGTTTCACTACCTCGTGTTTGACCTTGTTACCGGCGGGGAGCTGTTTGA
AGACATTGTGGCCAGAGAGTACTACAGTGAAGCAGATGCCAGCCACTGTATACATCAGAT
TCTGGAGAGTGTTAACCACATCCACCAGCATGACATCGTCCACAGGGACCTGAAGCCTGA
GAACCTGCTGCTGGCGAGTAAATGCAAGGGTGCCGCCGTCAAGCTGGCTGATTTTGGCCT
AGCCATCGAAGTACAGGGAGAGCAGCAGGCTTGGTTTGGTTTTGCTGGCACCCCAGGTTA
CTTGTCCCCTGAGGTCTTGAGGAAAGATCCCTATGGAAAACCTGTGGATATCTGGGCCTG
CGGGGTCATCCTGTATATCCTCCTGGTGGGCTATCCTCCCTTCTGGGATGAGGATCAGCA
CAAGCTGTATCAGCAGATCAAGGCTGGAGCCTATGATTTCCCATCACCAGAATGGGACAC
GGTAACTCCTGAAGCCAAGAACTTGATCAACCAGATGCTGACCATAAACCCAGCAAAGCG
CATCACGGCTGACCAGGCTCTCAAGCACCCGTGGGTCTGTCAACGATCCACGGTGGCATC
CATGATGCATCGTCAGGAGACTGTGGAGTGTTTGCGCAAGTTCAATGCCCGGAGAAAACT
GAAGGGTGCCATCCTCACGACCATGCTTGTCTCCAGGAACTTCTCAGTTGGCAGGCAGAG
CTCCGCCCCCGCCTCGCCTGCCGCGAGCGCCGCCGGCCTGGCCGGGCAAGCTGCCAAAAG
CCTATTGAACAAGAAGTCGGATGGCGGTGTCAAGAAAAGGAAGTCGAGTTCCAGCGTGCA
CCTAATGGAGCCACAAACCACTGTGGTACACAACGCTACAGATGGGATCAAGGGCTCCAC
AGAGAGCTGCAACACCACCACAGAAGATGAGGACCTCAAAGTGCGAAAACAGGAGATCAT
TAAGATTACAGAACAGCTGATTGAAGCCATCAACAATGGGGACTTTGAGGCCTACACGAA
GATTTGTGATCCAGGCCTCACTTCCTTTGAGCCTGAGGCCCTTGGTAACCTCGTGGAGGG
GATGGATTTCCATAAGTTTTACTTTGAGAATCTCCTGTCAAAGAACAGCAAGCCTATCCA
TACCACCATCCTAAACCCACACGTCCACGTGATTGGGGAGGACGCAGCGTGCATCGCCTA
CATCCGCCTCACCCAGTACATCGACGGGCAGGGTCGGCCTCGCACCAGCCAGTCAGAAGA
GACCCGGGTCTGGCACCGTCGGGATGGCAAGTGGCTCAATGTCCACTATCACTGCTCAGG
GGCCCCTGCCGCACCGCTGCAGTGAGCTCAGCCACAGGGGCTTTAGGAGATTCCAGCCGG
AGGTCCAACCTTCGCAGCCAGTGGCTCTGGAGGGCCTGAGTGACAGCGGCAGTCCTGTTT
GTTTGAGGTTTAAAACAATTCAATTACAAAAGCGGCAGCAGCCAATGCACGCCCCTGCAT
GCAGCCCTCCCGCCCGCCCTTCGTGTCTGTCTCTGCTGTACCGAGGTGTTTTTTACATTT
AAGAAAAAAAAAAAAGAAAAAAAGATTGTTTAAAAAAAAAAGGAATCCATACCATGATGC
GTTTTAAAACCACCGACAGCCCTTGGGTTGGCAAGAAGGCAGGAGTATGTATGAGGTCCA
TCCTGGCATGAGCAGTGGCTCACCCACCGGCCTTGAAGAGGTGAGCTTGGCCTCTCTGGT
CCCCATGGACTTAGGGGGACCAGGCAAGAACTCTGACAGAGCTTTGGGGGCCGTGATGTG
ATTGCAGCTCCTGAGGTGGCCTGCTTACCCCAGGTCTAGGAATGAACTTCTTTGGAACTT
GCATAGGCGCCTAGAATAGGGCTGATGAGAACATCGTGACCATCAGACCTACTTGGGAGA
GAACGCAGAGCTCCCAGCCTGCTGTGGAGGCAGCTGAGAAGTGGTGGCCTCAGGACTGAG
AGCCCGGACGTTGCTGTACTGTCTTGTTTAGTGTAGAAGGGAAGAGAATTGGTGCTGCAG
AAGTGTACCCGCCATGAAGCCGATGAGAAACCTCGTGTTAGTCTGACATGCACTCACTCA
TCCATTTCTATAGGATGCACAATGCATGTGGGCCCTAATATTGAGGCCTTATCCCTGCAG
CTAGGAGGGGGAGGGGTTGTTGCTGCTTTGCTTCGTGTTTTCTTCTAACCTGGCAAGGAG
AGAGCCAGGCCCTGGTCAGGGCTCCCGTGCCGCCTTTGGCGGTTCTGTTTCTGTGCTGAT
CTGGACCATCTTTGTCTTGCCTTTTCACGGTAGTGGTCCCCGTGCTGACCCTCATCTGGG
CCTGGGCCCTCTGCCAAGTGCCCCTGTGGGATGGGAGGAGTGAGGCAGTGGGAGAAGAGG
TGGTGGTCGTTTCTATGCATTCAGGCTGCCTTTGGGGCTGCCTCCCTTCTTATTCTTCCT
TGCTGCACGTCCATCTCTTTTCCTGTCTTTGAGATTGACCTGACTGCTCTGGCAAGAAGA
AGAGGTGTCCTTACAGAGGCCTCTTTACTGACCAACTGAAGTATAGACTTACTGCTGGAC
AATCTGCATGGGCATCACCCCTCCCCGCATGTAACCCAAAAGAGGTGTCCAGAGCCAAGG
CTTCTACCTTCATTGTCCCTCTCTGTGCTCAAGGAGTTCCATTCCAGGAGGAAGAGATCT
ATACCCTAAGCAGATAGCAAAGAAGATAATGGAGGAGCAATTGGTCATGGCCTTGGTTTC
CCTCAAAACAACGCTGCAGATTTATCTGCACAAACATCTCCACTTTTGGGGGAAAGGTGG
GTAGATTCCAGTTCCCTGGACTACCTTCAGGAGGCACGAGAGCTGGGAGAAGAGGCAAAG
CTACAGGTTTACTTGGGAGCCAGCTGAGAAGAGAGCAGACTCACAGGTGCTGGTGCTTGG
ATTTAGCCAGGCTCCTCAGAGCACCTCATGCATGTCCAAGCCCCTGGGCCCTAGCCCTTT
CCTGCCCTGCAGTCTGCAGTGCCAGCACGCAAATCCCTTCACCACAGGGTTTCGTTTTGC
TGGCTTGAAGACAAATGGTCTTAGAATTCATTGAGACCCATAGCTTCATATGGCTGCTCC
AGCCCCACTTCTTAGCATTCTTACTCCTCTTCTGGGGCTAATGTCAGCATCTATAGACAA
TAGACTATTAAAAAATCACCTTTTAAACAAGAAACGGAGGGCATTTGATGCAGAATTTTT
GCATGACAACATAGAAATAATTTAAAAATAGTGTTTGTTCTGAATGTTGGTAGACCCTTC
ATAGCTTTGTTACAATGAAACCTTGAACTGAAAATATTTAATAAAATAACCTTTAAACAG
TCAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MATTATCTRFTDDYQLFEELGKGAFSVVRRCVKKTPTQEYAAKIINTKKLSARDHQKLER EARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIHQI LESVNHIHQHDIVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGEQQAWFGFAGTPGY LSPEVLRKDPYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDT VTPEAKNLINQMLTINPAKRITADQALKHPWVCQRSTVASMMHRQETVECLRKFNARRKL KGAILTTMLVSRNFSVGRQSSAPASPAASAAGLAGQAAKSLLNKKSDGGVKKRKSSSSVH LMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTK ICDPGLTSFEPEALGNLVEGMDFHKFYFENLLSKNSKPIHTTILNPHVHVIGEDAACIAY IRLTQYIDGQGRPRTSQSEETRVWHRRDGKWLNVHYHCSGAPAAPLQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
