Gene:
Gene Symbol:
PKN1
Gene Name:
protein kinase N1
Original Clone ID:
FLH230757.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618026
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2829nts
Open reading frame: 166 to 2994
CGGCGGCCTGGCCAGGCGAGGCGGGAGCCGAGCAGGCCGGAGCGGGCCGCGCGCCGGGGA
GGGTCGCGCCGCGCCCGCCCCCTCCCCGCCACGCCCGGGGCGCACGCGCGCTCCTCTGGC
CGCCCCTCCCTCCGCGCGGGGACCCCTGGCGGACGGCAGGAGGACATGGCCAGCGACGCC
GTGCAGAGTGAGCCTCGCAGCTGGTCCCTGCTAGAGCAGCTGGGCCTGGCCGGGGCAGAC
CTGGCGGCCCCCGGGGTACAGCAGCAGCTGGAGCTGGAGCGGGAGCGGCTGCGGCGGGAA
ATCCGCAAGGAGCTGAAGCTGAAGGAGGGTGCTGAGAACCTGCGGCGGGCCACCACTGAC
CTGGGCCGCAGCCTGGGCCCCGTAGAGCTGCTGCTGCGGGGCTCCTCGCGCCGCCTCGAC
CTGCTGCACCAGCAGCTGCAGGAGCTGCACGCCCACGTGGTGCTTCCCGACCCGGCGGCC
ACCCACGATGGCCCCCAGTCCCCTGGTGCGGGTGGCCCCACCTGCTCGGCCACCAACCTG
AGCCGCGTGGCGGGCCTGGAGAAGCAGTTGGCCATTGAGCTGAAGGTGAAGCAGGGGGCG
GAGAACATGATCCAGACCTACAGCAATGGCAGCACCAAGGACCGGAAGCTGCTGCTGACA
GCCCAGCAGATGTTGCAGGACAGTAAGACCAAGATTGACATCATCCGCATGCAACTCCGC
CGGGCGCTGCAGGCCGGCCAGCTGGAGAACCAGGCAGCCCCGGATGACACCCAAGGGAGT
CCTGACCTGGGGGCTGTGGAGCTGCGCATCGAAGAGCTGCGGCACCACTTCCGAGTGGAG
CACGCGGTGGCCGAGGGTGCCAAGAACGTACTGCGCCTGCTCAGCGCTGCCAAGGCCCCG
GACCGCAAGGCAGTCAGCGAGGCCCAGGAGAAATTGACAGAATCCAACCAGAAGCTGGGG
CTGCTGCGGGAGGCTCTGGAGCGGAGACTTGGGGAGCTGCCCGCCGACCACCCCAAGGGG
CGGCTGCTGCGAGAAGAGCTCGCTGCGGCCTCCTCCGCTGCCTTCAGCACCCGCCTGGCC
GGGCCCTTTCCCGCCACGCACTACAGCACCCTGTGCAAGCCCGCGCCGCTCACAGGGACC
CTGGAGGTACGAGTGGTGGGCTGCAGAGACCTCCCAGAGACCATCCCGTGGAACCCTACC
CCCTCAATGGGGGGACCTGGGACCCCAGACAGCCGCCCCCCCTTCCTGAGCCGCCCAGCC
CGGGGCCTTTACAGCCGAAGCGGAAGCCTCAGTGGCCGGAGCAGCCTCAAAGCAGAAGCC
GAGAACACCAGTGAAGTCAGCACTGTGCTTAAGCTGGATAACACAGTGGTGGGGCAGACG
TCTTGGAAGCCATGTGGCCCCAATGCCTGGGACCAGAGCTTCACTCTGGAGCTGGAAAGG
GCACGGGAACTGGAGTTGGCTGTGTTCTGGCGGGACCAGCGGGGCCTGTGTGCCCTCAAA
TTCCTGAAGTTGGAGGATTTCTTGGACAATGAGAGGCATGAGGTGCAGCTGGACATGGAA
CCCCAGGGCTGCCTGGTGGCTGAGGTCACCTTCCGCAACCCTGTCATTGAGAGGATTCCT
CGGCTCCGACGGCAGAAGAAAATTTTCTCCAAGCAGCAAGGGAAGGCGTTCCAGCGTGCT
AGGCAGATGAACATCGATGTCGCCACGTGGGTGCGGCTGCTCCGGAGGCTCATCCCCAAT
GCCACGGGCACAGGCACCTTTAGCCCTGGGGCTTCTCCAGGATCCGAGGCCCGGACCACG
GGTGACATATCGGTGGAGAAGCTGAACCTCGGCACTGACTCGGACAGCTCACCTCAGAAG
AGCTCGCGGGATCCTCCTTCCAGCCCATCGAGCCTGAGCTCCCCCATCCAGGAATCCACT
GCTCCCGAGCTGCCTTCGGAGACCCAGGAGACCCCAGGCCCCGCCCTGTGCAGCCCTCTG
AGGAAGTCACCTCTGACCCTCGAAGATTTCAAGTTCCTGGCGGTGCTGGGCCGGGGTCAT
TTTGGGAAGGTGCTCCTCTCCGAATTCCGGCCCAGTGGGGAGCTGTTCGCCATCAAGGCT
CTGAAGAAAGGGGACATTGTGGCCCGAGACGAGGTGGAGAGCCTGATGTGTGAGAAGCGG
ATATTGGCGGCAGTGACCAGTGCGGGACACCCCTTCCTGGTGAACCTCTTCGGCTGTTTC
CAGACACCGGAGCACGTGTGCTTCGTGATGGAGTACTCGGCCGGTGGGGACCTGATGCTG
CACATCCACAGCGACGTGTTCTCTGAGCCCCGTGCCATCTTTTATTCCGCCTGCGTGGTG
CTGGGCCTACAGTTTCTTCACGAACACAAGATCGTCTACAGGGACCTGAAGTTGGACAAT
TTGCTCCTGGACACCGAGGGCTACGTCAAGATCGCAGACTTTGGCCTCTGCAAGGAGGGT
ATGGGCTATGGGGACCGGACCAGCACATTCTGTGGGACCCCGGAGTTCCTGGCCCCTGAG
GTGCTGACGGACACGTCGTACACGCGAGCTGTGGACTGGTGGGGACTGGGTGTGCTGCTC
TACGAGATGCTGGTTGGCGAGTCCCCATTCCCAGGGGATGATGAGGAGGAGGTCTTCGAC
AGCATCGTCAACGACGAGGTTCGCTACCCCCGCTTCCTGTCGGCCGAAGCCATCGGCATC
ATGAGAAGGCTGCTTCGGAGGAACCCAGAGCGGAGGCTGGGATCTAGCGAGAGAGATGCA
GAAGATGTGAAGAAACAGCCCTTCTTCAGGACTCTGGGCTGGGAAGCCCTGTTGGCCCGG
CGCCTGCCACCGCCCTTTGTGCCCACGCTGTCCGGCCGCACCGACATCAGCAACTTCGAC
GAGGAGTTCACCGGGGAGGCCCCCACACTGAGCCCGCCCCGCGACGCGCGGCCCCTCACA
GCCGCGGAGCAGGCAGCCTTCCTGGACTTCGACTTCGTGGCCGGGGGCTGCTAGCCCCCT
CCCCTGCCCCTGCCCCTGCCCCTGCCCGAGAGCTCTTAGTTTTTAAAAAGGCCTTTGGGA
TTTGCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLR RATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTC SATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDII RMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLS AAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAF STRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPF LSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFT LELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPV IERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGS EARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPA LCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESL MCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFY SACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPE FLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSA EAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTD ISNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
