Gene:
Gene Symbol:
EEF2K
Gene Name:
eukaryotic elongation factor-2 kinase
Original Clone ID:
HsCD00298626
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618027
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2178nts
Open reading frame: 197 to 2374
CCACGCGTCCGGGCCCCTGCGCCCTTCCTGGGATCACTCCGACTGCCCCGCGCCTCTGCC
AACTCCTCTGGACCCTCGCGGCCGTGGGCAGCGGCTGCCGCGCCTGTCTGCCCGAGGGAG
GACCTTCGCCTCTGCATTTGTCCAGTAACTCTGGCTGTGCCGGATACTGCTTGGGTAAAA
CGGGCACCCCAGGAACATGGCAGACGAAGATCTCATCTTCCGCCTGGAAGGCGTTGATGG
CGGCCAGTCCCCCCGAGCTGGCCGTGATGGTGATTCTGATGGGGACAGCGACGATGAGGA
AGGTTACTTCATCTGCCCCATCACGGATGACCCAAGCTCGAACCAGAATGTCAATTCCAA
GGTTAATAAGTACTACAGCAACCTAACAAAAAGTGAGCGGTATAGCTCCAGCGGGTCCCC
GGCAAACTCCTTCCACTTCAAGGAAGCCTGGAAGCACGCAATCCAGAAGGCCAAGCACAT
GCCCGACCCCTGGGCTGAGTTCCACCTGGAAGATATTGCCACCGAACGTGCTACTCGACA
CAGGTACAACGCCGTCACCGGGGAATGGCTGGATGATGAAGTTCTGATCAAGATGGCATC
TCAGCCCTTCGGCCGAGGAGCAATGAGGGAGTGCTTCCGGACGAAGAAGCTCTCCAACTT
CTTGCATGCCCAGCAGTGGAAGGGAGCCTCCAACTACGTGGCGAAGCGCTACATCGAGCC
CGTAGACCGGGATGTGTACTTTGAGGACGTGCGTCTACAGATGGAGGCCAAGCTCTGGGG
GGAGGAGTATAATCGGCACAAGCCCCCCAAGCAGGTGGACATCATGCAGATGTGCATCAT
CGAGCTGAAGGACAGACCGGGCAAGCCCCTCTTCCACCTGGAGCACTACATCGAGGGCAA
GTACATCAAGTACAACTCCAACTCTGGCTTTGTCCGCGATGACAACATCCGCCTGACGCC
GCAGGCCTTCAGCCACTTCACTTTTGAGCGTTCCGGCCATCAGCTGATAGTGGTGGACAT
CCAGGGAGTTGGGGATCTCTACACTGACCCACAGATCCACACGGAGACGGGCACTGACTT
TGGAGACGGCAACCTAGGTGTCCGCGGGATGGCGCTCTTCTTCTACTCTCATGCCTGCAA
CCGGATTTGCGAGAGCATGGGCCTTGCTCCCTTTGACCTCTCGCCCCGGGAGAGGGATGC
AGTGAATCAGAACACCAAGCTGCTGCAATCAGCCAAGACCATCTTGAGAGGAACAGAGGA
AAAATGTGGGAGCCCCCGAGTAAGGACCCTCTCTGGGAGCCGGCCACCCCTGCTCCGTCC
CCTTTCAGAGAACTCTGGAGACGAGAACATGAGCGACGTGACCTTCGACTCTCTCCCTTC
TTCCCCATCTTCGGCCACACCACACAGCCAGAAGCTAGACCACCTCCATTGGCCAGTGTT
CAGTGACCTCGATAACATGGCATCCAGAGACCATGATCATCTAGACAACCACCGGGAGTC
TGAGAATAGTGGGGACAGCGGATACCCCAGTGAGAAGCGGGGTGAGCTGGATGACCCTGA
GCCCCGAGAACATGGCCACTCATACAGTAATCGGAAGTACGAGTCTGACGAAGACAGCCT
GGGCAGCTCTGGACGGGTATGTGTAGAGAAGTGGAATCTCCTCAACTCCTCCCGCCTCCA
CCTGCCGAGGGCTTCGGCCGTGGCCCTGGAAGTGCAAAGGCTTAATGCTCTGGACCTCGA
AAAGAAAATCGGGAAGTCCATTTTGGGGAAGGTCCATCTGGCCATGGTGCGCTACCACGA
GGGTGGGCGCTTCTGCGAGAAGGGCGAGGAGTGGGACCAGGAGTCGGCTGTCTTCCACCT
GGAGCACGCAGCCAACCTGGGCGAGCTGGAGGCCATCGTGGGCCTGGGACTCATGTACTC
GCAGTTGCCTCATCACATCCTAGCCGATGTCTCTCTGAAGGAGACAGAAGAGAACAAAAC
CAAAGGATTTGATTACTTACTAAAGGCCGCTGAAGCTGGCGACAGGCAGTCCATGATCCT
AGTGGCGCGAGCTTTTGACTCTGGCCAGAACCTCAGCCCGGACAGGTGCCAAGACTGGCT
AGAGGCCCTGCACTGGTACAACACTGCCCTGGAGATGACGGACTGTGATGAGGGCGGTGA
GTACGACGGAATGCAGGACGAGCCCCGGTACATGATGCTGGCCAGGGAGGCCGAGATGCT
GTTCACAGGAGGCTACGGGCTGGAGAAGGACCCGCAGAGATCAGGGGACTTGTATACCCA
GGCAGCAGAGGCAGCGATGGAAGCCATGAAGGGCCGACTGGCCAACCAGTACTACCAAAA
GGCTGAAGAGGCCTGGGCCCAGATGGAGGAGTAACCAGGAAAATCACTGCCGGCTAGTCC
CAAGCAAACGGGCTAGGAGGAAAGATTAAAAAAACAACAACAACAACTTATTTAGTTTGG
GGAGGGGAAGCATTTTTAAGTGTGTTGTAAAATCAAATTTTATATTTCATTTTTTGACTC
TTGAAAAATGTCTTTGCTCCTTGGCAGCTACCAGCAGAGACTCTATAGCTGTCTCTTAGG
GCAGTATTTTGGGGAAGTGGGGCTTGAAGAAGCAGCCTAATGAACCAACATACCGTTTTG
TGTGTGGTTTTTTTTGTTTGTTTGTTTGTTTGTTTTGAGACAGAGTCTTGCTCTGTCACC
CAGGCTGGAGTGCAGTGACATGATCTTAGCTCACTGCAACCTCCGCCTCCTGGGTTCAAG
TGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACTGGTGCACACCACCACACTCA
GCTAATTTTTGCATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGA
ACTCCTGGACTTAAGTGAGCCTCCCGCCTCAGTCTCCCAAAGCGCTGGGATTGCAGGCAG
GAGCCACTGAGCCCAGCCAAGACTTCAGTGTTGACTGCTTTGGAGGCACAAACCCATGCA
AGCGTTAGTTCCAAAGTTCAGTGTGTACCCTTAAATGAACAATGAAGCAGGTAAAATTAC
CCTTGAAAAAAATCCCTTGGACCACCCATAAATGACAGTGACTTTTTCAATATGGACTCA
TCATAGCCAGTTTTCCTTTTGAAGTTGGAACTGATCACCCTTTTGTCATCTGTACCAGAT
CAGTAGTTGGCTTGTGTTACATTTTGTGTGTGTGTGTGCGTGTTTTAAACCAGTGCATAT
AAATTGTATGTTAAATGTAAGTAACTTTAAGTTGACTTATCTCTTCACAGTAATCAAGCC
TCACGTAATTCATGCTTTTTAAATTCAGCCAGCCCCCCCTCTCTGAAATTTTATTATGTA
AATAATTTGTGTTCCCTGATCACTCGTTTAAGTTCTTAGTTGTATGTCATCTCTTCTCTA
GCAGGAATTGGCAAACTTTTTTGTAAAGGGGTAGAAAGTGAAGATTTTAGGCTTTGCAGG
CCATATAGCCTCTGCTGCAAATGCTCAGCCCTGCTGTTGTAATGTAAAAGCTGCCACAGA
CACTACATGAACACGAATGAGTGTGGCTGGTGTTCCAATAAAACTTTATTTACACAAAAA
AAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MADEDLIFRLEGVDGGQSPRAGRDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYY SNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAV TGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDV YFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYN SNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNL GVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSP RVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDN MASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGR VCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFC EKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDY LLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQ DEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAW AQMEE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
