Gene:
Gene Symbol:
PHKA1
Gene Name:
phosphorylase kinase, alpha 1 (muscle)
Original Clone ID:
FLH230833.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618046
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GCCGCCGGGCGCCAGGCCTGAGCGGTGGGAGGGCTCTGCGGGGCCTGGTGTTCAGGCGTC
CCACCACGAGGGTGGAGCAGCGTTGGATACTTGTTCCTTAGGGACCGAAGCTCCGGTGGC
ACCCGGGCTATTTCTCAGAGGACAATTAGTAACGTGTCGCCATGAGGAGCCGGAGTAACT
CCGGGGTCCGGCTGGACGGCTACGCTCGACTGGTGCAACAGACCATCCTGTGCCATCAGA
ATCCAGTGACTGGCTTGCTTCCAGCCAGCTATGATCAGAAAGATGCTTGGGTCCGAGATA
ATGTGTACAGCATCTTGGCTGTGTGGGGTTTGGGCCTGGCCTATCGGAAGAATGCAGACC
GGGATGAGGATAAGGCAAAGGCCTATGAATTGGAGCAGAGTGTAGTGAAGCTGATGAGAG
GACTACTGCACTGCATGATCAGACAGGTGGATAAAGTAGAATCCTTCAAATATAGTCAGA
GTACTAAGGATAGCCTCCATGCAAAGTACAACACCAAAACCTGTGCCACTGTAGTGGGTG
ATGATCAATGGGGACACCTGCAGTTGGATGCTACCTCTGTGTACCTGCTCTTCTTAGCCC
AAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGATGAAGTCAATTTCATACAGA
ACCTTGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTCGGGATATGGGAACGTG
GAGACAAGACCAACCAAGGGATCTCAGAGTTGAATGCCAGTTCAGTTGGAATGGCAAAGG
CAGCCCTGGAAGCATTAGATGAACTGGATCTGTTTGGTGTGAAAGGTGGGCCTCAATCAG
TTATCCATGTCCTGGCTGATGAAGTACAGCACTGCCAGTCTATCCTAAATTCACTACTGC
CCCGTGCTTCAACATCAAAAGAGGTTGATGCTAGTCTACTCTCAGTGGTTTCCTTCCCTG
CCTTTGCAGTAGAGGATAGCCAGTTGGTGGAGCTCACAAAACAGGAAATCATCACCAAGC
TTCAGGGTCGTTATGGTTGCTGTCGCTTTCTACGAGATGGATATAAAACTCCTAAAGAGG
ATCCCAATCGTCTGTACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGTG
AATGGCCATTGTTCTGGACATACTTTATTCTTGATGGGGTCTTCAGTGGCAATGCAGAAC
AGGTTCAAGAATATAAAGAGGCTCTTGAAGCAGTCCTCATCAAGGGCAAAAATGGAGTCC
CACTTCTGCCAGAGCTGTACAGTGTTCCTCCTGACAGGGTCGATGAAGAATATCAGAATC
CTCACACTGTGGACCGAGTCCCCATGGGGAAATTGCCTCACATGTGGGGTCAGTCTCTAT
ACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCCCCTGGAGAAATTGATCCCCTGA
ATCGCAGGTTTTCTACTGTACCGAAGCCCGATGTTGTGGTTCAAGTCTCCATTCTAGCTG
AAACAGAAGAAATCAAGACCATTTTGAAGGACAAGGGAATTTACGTGGAGACCATTGCTG
AGGTATACCCCATCAGAGTACAACCAGCTCGTATTCTCAGCCACATTTATTCCAGCCTAG
GATGCAACAATAGAATGAAACTCAGTGGACGACCCTACAGACACATGGGAGTGCTTGGAA
CTTCAAAACTCTATGACATTCGGAAAACTATCTTTACTTTCACTCCACAGTTTATAGACC
AGCAACAGTTCTACCTGGCTCTGGACAACAAGATGATAGTGGAAATGCTTAGAACAGACC
TCTCCTACCTCTGTAGCCGCTGGCGGATGACAGGCCAGCCCACCATCACCTTCCCCATCT
CATACAGCATGCTTGATGAAGATGGAACAAGCTTGAATTCAAGTATCCTGGCAGCACTCC
GAAAAATGCAAGATGGGTATTTTGGTGGGGCAAGGGTTCAAACAGGTAAATTGTCAGAGT
TTTTGACAACATCTTGTTGCACACACTTGAGCTTCATGGACCCTGGACCTGAGGGTAAGC
TGTACAGTGAAGATTATGATGACAACTATGATTACCTGGAATCTGGCAACTGGATGAATG
ATTATGATTCAACCAGTCATGCTCGCTGTGGTGATGAAGTTGCTCGTTATTTAGATCACC
TTTTGGCGCACACTGCTCCCCATCCTAAACTAGCCCCTACCTCACAGAAGGGAGGGCTAG
ATCGGTTCCAAGCTGCTGTGCAAACAACCTGCGACTTAATGTCCTTGGTGACCAAGGCCA
AGGAACTGCATGTACAGAATGTTCACATGTATCTTCCTACGAAGTTATTTCAGGCTTCCC
GGCCTTCATTCAACTTACTTGATTCACCTCATCCCCGACAGGAGAACCAGGTTCCCTCTG
TTCGTGTAGAAATACATCTTCCTAGAGACCAGTCTGGGGAGGTGGACTTTAAAGCACTGG
TTTTACAGTTGAAGGAGACCTCAAGCTTACAGGAACAAGCTGATATCCTCTATATGCTGT
ATACTATGAAAGGACCTGACTGGAACACTGAATTGTATAATGAACGGAGTGCTACAGTGA
GAGAGCTTCTTACCGAGCTGTATGGCAAAGTGGGAGAAATTCGTCACTGGGGCCTGATCC
GATACATTTCTGGGATCTTAAGGAAGAAAGTGGAAGCACTTGATGAGGCCTGCACAGACC
TTCTCTCCCACCAGAAACATTTGACAGTAGGACTTCCTCCAGAACCTCGAGAAAAGACTA
TCTCTGCACCTCTGCCCTATGAGGCGCTCACTCAGCTGATAGATGAAGCCAGTGAAGGGG
ATATGAGCATTTCAATCCTTACACAGGAAATAATGGTATATCTAGCCATGTATATGCGAA
CCCAGCCTGGCCTCTTTGCTGAAATGTTTCGACTTCGAATTGGTCTGATCATACAAGTTA
TGGCAACAGAACTGGCCCACTCCCTTCGATGCTCAGCTGAGGAAGCCACAGAGGGCCTGA
TGAATCTCAGTCCTTCGGCCATGAAGAATCTCCTGCATCACATTCTCAGCGGCAAGGAGT
TTGGAGTGGAACGAAGCGTTCGTCCCACTGATTCAAATGTCAGTCCTGCTATTTCTATCC
ACGAGATTGGTGCTGTTGGAGCAACCAAAACAGAACGAACTGGGATCATGCAGTTAAAAA
GTGAGATAAAGCAGGTGGAATTTCGTAGACTGTCAATCTCAGCTGAGAGTCAGTCACCTG
GAACCTCTATGACTCCAAGTAGTGGGTCCTTTCCTAGTGCATATGATCAGCAGTCATCTA
AAGATAGTCGTCAAGGTCAATGGCAACGCCGAAGAAGGCTGGATGGGGCACTGAATAGAG
TTCCAGTTGGATTTTATCAGAAAGTATGGAAAGTTTTGCAGAAGTGTCACGGACTTTCTG
TTGAAGGGTTTGTCCTTCCTTCCTCTACCACTAGAGAGATGACTCCAGGTGAGATTAAAT
TCTCTGTTCATGTGGAGTCTGTCCTGAATCGTGTACCTCAGCCAGAGTACCGTCAGCTGC
TGGTTGAAGCCATCCTTGTCCTCACCATGCTGGCAGATATTGAAATTCATAGCATCGGAA
GCATCATTGCTGTGGAAAAAATAGTGCATATTGCCAATGACTTGTTCCTTCAAGAACAGA
AAACCCTTGGCGCAGATGATACCATGTTGGCAAAGGATCCCGCATCTGGCATCTGTACTC
TTCTGTATGACAGTGCACCCAGTGGCAGGTTTGGCACCATGACCTACCTCTCCAAGGCAG
CCGCCACCTACGTGCAGGAGTTCCTGCCCCACAGCATCTGTGCCATGCAATGAGGGCTTT
GGTTCCTGGCTTCTGGGAGCCTTTTGACAGCTGGTCCCTGCCTCGGTTGATTGTGCATGG
AACTAAAATGTTATTGCCTAATCACTCCAACCCTGCCCCTTTCTGTCCCATCCTTCCCAA
GAAGAGAGAACTTTTTCGATAAACTAACTACTGTAGAAGAAGTGAACACTTACCTGGAGG
CTCACCTTGCAGAACCAGTGACAATCTTATGAGTATAATGAACACTCAGCCAGGCCTGTC
ATGATTGGCTTTATTTCTTTCATCATTCATAAAAGTTTGCATGTGTTTTTATTCTCTAGA
TCTGTTACCAATATAGTTTTCTAACTCCTGTTTGGGGAGCAAGTGTTAATAATAACTTAT
TCCTAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLA YRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKT CATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTAD FGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQS ILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSQLVELTKQEIITKLQGRYGCCRFLRDG YKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQEYKEALEAVLI KGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAP GEIDPLNRRFSTVPKPDVVVQVSILAETEEIKTILKDKGIYVETIAEVYPIRVQPARILS HIYSSLGCNNRMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNKMIV EMLRTDLSYLCSRWRMTGQPTITFPISYSMLDEDGTSLNSSILAALRKMQDGYFGGARVQ TGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDDNYDYLESGNWMNDYDSTSHARCGDEV ARYLDHLLAHTAPHPKLAPTSQKGGLDRFQAAVQTTCDLMSLVTKAKELHVQNVHMYLPT KLFQASRPSFNLLDSPHPRQENQVPSVRVEIHLPRDQSGEVDFKALVLQLKETSSLQEQA DILYMLYTMKGPDWNTELYNERSATVRELLTELYGKVGEIRHWGLIRYISGILRKKVEAL DEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEALTQLIDEASEGDMSISILTQEIMVY LAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHH ILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSIS AESQSPGTSMTPSSGSFPSAYDQQSSKDSRQGQWQRRRRLDGALNRVPVGFYQKVWKVLQ KCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADI EIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTM TYLSKAAATYVQEFLPHSICAMQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
