Gene:
Gene Symbol:
PRKCH
Gene Name:
protein kinase C, eta
Original Clone ID:
HsCD00298693
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618048
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2052nts
Open reading frame: 389 to 2440
AGCGGGAAGAGACGCTTGGGGCTGGGGCTCACCGGACGGGTAGGTCCGGCTCTCCAGGGA
GAGGAGCTGCCCGGCCCTGGAGAAGGGGCGAGTCCTGCGCGAGTCCCCGGGAGGCGCCGC
GCGCTTGGAAGGGACGGTCGGGCTTCCCCGGCCCGCTGAGGGCTAGGCGGCGGGCTCCCC
TCCTTTCCACCTCGGCAGGGAGGGAAGGAGGGGAGGGAAAAGTCCCACGGAGGAGGCAGA
ATGGCCAGTCGAGGGGCGCTTAGGCGCTGCCTTTCCCCAGGGCTGCCTCGACTCCTGCAC
CTGTCCCGAGGGCTGGCCTGAGACGGGACTCCCGGTTCTCCCGCTGCGAAGCAGCGCGGC
CCCCCGGGGCCGGGGCAGCGGCGCCGGCATGTCGTCTGGCACCATGAAGTTCAATGGCTA
TTTGAGGGTCCGCATCGGTGAGGCAGTGGGGCTGCAGCCCACCCGCTGGTCCCTGCGCCA
CTCGCTCTTCAAGAAGGGCCACCAGCTGCTGGACCCCTATCTGACGGTGAGCGTGGACCA
GGTGCGCGTGGGCCAGACCAGCACCAAGCAGAAGACCAACAAACCCACGTACAACGAGGA
GTTTTGCGCTAACGTCACCGACGGCGGCCACCTCGAGTTGGCCGTCTTCCACGAGACGCC
CCTGGGCTACGACCACTTCGTGGCCAACTGCACCCTGCAGTTCCAGGAGCTGCTGCGCAC
GACCGGCGCCTCGGACACCTTCGAGGGTTGGGTGGATCTCGAGCCAGAGGGGAAAGTATT
CGTGGTAATAACCCTTACCGGGAGTTTCACTGAAGCTACTCTCCAGAGAGACCGGATCTT
CAAACATTTTACCAGGAAGCGCCAAAGGGCTATGCGAAGGCGAGTCCACCAGATCAATGG
ACACAAGTTCATGGCCACGTATCTGAGGCAGCCCACCTACTGCTCTCACTGCAGGGAGTT
TATCTGGGGAGTGTTTGGGAAACAGGGTTATCAGTGCCAAGTGTGCACCTGTGTCGTCCA
TAAACGCTGCCATCATCTAATTGTTACAGCCTGTACTTGCCAAAACAATATTAACAAAGT
GGATTCAAAGATTGCAGAACAGAGGTTCGGGATCAACATCCCACACAAGTTCAGCATCCA
CAACTACAAAGTGCCAACATTCTGCGATCACTGTGGCTCACTGCTCTGGGGAATAATGCG
ACAAGGACTTCAGTGTAAAATATGTAAAATGAATGTGCATATTCGATGTCAAGCGAACGT
GGCCCCTAACTGTGGGGTAAATGCGGTGGAACTTGCCAAGACCCTGGCAGGGATGGGTCT
CCAACCCGGAAATATTTCTCCAACCTCGAAACTCGTTTCCAGATCGACCCTAAGACGACA
GGGAAAGGAGAGCAGCAAAGAAGGAAATGGGATTGGGGTTAATTCTTCCAACCGACTTGG
TATCGACAACTTTGAGTTCATCCGAGTGTTGGGGAAGGGGAGTTTTGGGAAGGTGATGCT
TGCAAGAGTAAAAGAAACAGGAGACCTCTATGCTGTGAAGGTGCTGAAGAAGGACGTGAT
TCTGCAGGATGATGATGTGGAATGCACCATGACCGAGAAAAGGATCCTGTCTCTGGCCCG
CAATCACCCCTTCCTCACTCAGTTGTTCTGCTGCTTTCAGACCCCCGATCGTCTGTTTTT
TGTGATGGAGTTTGTGAATGGGGGTGACTTGATGTTCCACATTCAGAAGTCTCGTCGTTT
TGATGAAGCACGAGCTCGCTTCTATGCTGCAGAAATCATTTCGGCTCTCATGTTCCTCCA
TGATAAAGGAATCATCTATAGAGATCTGAAACTGGACAATGTCCTGTTGGACCACGAGGG
TCACTGTAAACTGGCAGACTTCGGAATGTGCAAGGAGGGGATTTGCAATGGTGTCACCAC
GGCCACATTCTGTGGCACGCCAGACTATATCGCTCCAGAGATCCTCCAGGAAATGCTGTA
CGGGCCTGCAGTAGACTGGTGGGCAATGGGCGTGTTGCTCTATGAGATGCTCTGTGGTCA
CGCGCCTTTTGAGGCAGAGAATGAAGATGACCTCTTTGAGGCCATACTGAATGATGAGGT
GGTCTACCCTACCTGGCTCCATGAAGATGCCACAGGGATCCTAAAATCTTTCATGACCAA
GAACCCCACCATGCGCTTGGGCAGCCTGACTCAGGGAGGCGAGCACGCCATCTTGAGACA
TCCTTTTTTTAAGGAAATCGACTGGGCCCAGCTGAACCATCGCCAAATAGAACCGCCTTT
CAGACCCAGAATCAAATCCCGAGAAGATGTCAGTAATTTTGACCCTGACTTCATAAAGGA
AGAGCCAGTTTTAACTCCAATTGATGAGGGACATCTTCCAATGATTAACCAGGATGAGTT
TAGAAACTTTTCCTATGTGTCTCCAGAATTGCAACCATAGCCTTATGGGGAGTGAGAGAG
AGGGCACGAGAACCCAAAGGGAATAGAGATTCTCCAGGAATTTCCTCTATGGGACCTTCC
CAGCATCAGCCTTAGAACAAGAACCTTACCTTCAAGGAGCAAGTGAAGAACTCTGTGAAG
GATGGAACTTTCAGATATCAACTATTTAGAGTCCAGAGGGAGCCATGGCACTAGAAATAG
TTGATAATGAAATGAGATTTTATGAAGTATACCGCTCCACCTATGAGCGTCTGTCTCTGT
GGGCTTGGGATGTTAACAGGAGCCAAAAGGAGGGAAAGTGTGAAGAATAAAGTAGATCTG
AGAAATTCTGAGCCAATCAGGCTTCTTAATTCAAGAGACAAACCAAGACGTTCTGTCAAC
TGTGCTGTGCTCTTCTTTAAGCCAATGAACCCCAATTCCTGGCAGTCTACAAGAAGTCTC
TTAATGCTAATGAAGAATTTAAAGGTCTTTTTAAGGAAATGAAGGGCTTTCCAAATAGAA
TGATTTACTCTGAAGAAACAAACAATGGTATCTCTGAAACTCACAACCTAAAGCCCAATC
TTGAAAATATGTTGTGCACCAAGACGACTGCTTCAGCTTCTTCTCTTATCCTTACTTTCT
TTAATAGATATTTATTAAACTGTCCAGTGAAAAGGTGCCACAATGCCCAGTATTGTAAAC
AACAGGTTTGCATTCATGAAGCTTTCATTCATTCTGGAGTCTACTAATTTACCTGAATGG
TGTTTGCATTCTGTGAAATGCCTCTCCACGTTGCATATGTCACACTTTTGTCTGCACATA
ACTCTTTTTTCACAAGAAGGGTCACTGCCACAACAGCACAGTCAGCGGGTGAATTACAGG
TGCCTGCTGCCTGCCTACCTGGGTAATCTGATCTTGTCTGTATCGCCGTGTGCTCATCAC
TGAAGAATTGCAGGCCACTCATGTCAGTGACCAGATTTGTGGCTTATAAACATTAGCAGT
TTATTTATGTTTTAAGATGCAAAGATGTGTGTTTGATATTCACTTTAATAATTAGAAATG
GATCTTGTAAACAGGGCATATATCAAAGATGACCTTATAATATGTACCCGAATATACAGT
TCAAGAATTTTGTCTGACTGGAAATAAATGCATTTTGTAGCAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTK QKTNKPTYNEEFCANVTDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTTGASDTFEG WVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQINGHKFMATYLR QPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLIVTACTCQNNINKVDSKIAEQRF GINIPHKFSIHNYKVPTFCDHCGSLLWGIMRQGLQCKICKMNVHIRCQANVAPNCGVNAV ELAKTLAGMGLQPGNISPTSKLVSRSTLRRQGKESSKEGNGIGVNSSNRLGIDNFEFIRV LGKGSFGKVMLARVKETGDLYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLF CCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHDKGIIYRDL KLDNVLLDHEGHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAM GVLLYEMLCGHAPFEAENEDDLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSL TQGGEHAILRHPFFKEIDWAQLNHRQIEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDE GHLPMINQDEFRNFSYVSPELQP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
