DNASU Plasmid | MAP2K6 (H. sapiens )<br>In pLDNT7_nFLAG (Flag-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: MAP2K6

Gene Name: mitogen-activated protein kinase kinase 6

Original Clone ID:

HsCD00298726

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLDNT7_nFLAG

Format:

CLOSED

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00618062

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1005nts

Open reading frame: 341 to 1345

GGCTTCTGGTTCGGCCCACCTCTGAAGGTTCCAGAATCGATAGTGAATTCGTGGTTCCAA
GTTTGGAGCTTTTAGCTGCCAGCCCTGGCCCATCATGTAGCTGCAGCACAGCCTTCCCTA
ACGTTGCAACTGGGGGAAAAATCACTTTCCAGTCTGTTTTGCAAGGTGTGCATTTCCATC
TTGATTCCCTGAAAGTCCATCTGCTGCATCGGTCAAGAGAAACTCCACTTGCATGAAGAT
TGCACGCCTGCAGCTTGCATCTTTGTTGCAAAACTAGCTACAGAAGAGAAGCAAGGCAAA
GTCTTTTGTGCTCCCCTCCCCCATCAAAGGAAAGGGGAAAATGTCTCAGTCGAAAGGCAA
GAAGCGAAACCCTGGCCTTAAAATTCCAAAAGAAGCATTTGAACAACCTCAGACCAGTTC
CACACCACCTCGAGATTTAGACTCCAAGGCTTGCATTTCTATTGGAAATCAGAACTTTGA
GGTGAAGGCAGATGACCTGGAGCCTATAATGGAACTGGGACGAGGTGCGTACGGGGTGGT
GGAGAAGATGCGGCACGTGCCCAGCGGGCAGATCATGGCAGTGAAGCGGATCCGAGCCAC
AGTAAATAGCCAGGAACAGAAACGGCTACTGATGGATTTGGATATTTCCATGAGGACGGT
GGACTGTCCATTCACTGTCACCTTTTATGGCGCACTGTTTCGGGAGGGTGATGTGTGGAT
CTGCATGGAGCTCATGGATACATCACTAGATAAATTCTACAAACAAGTTATTGATAAAGG
CCAGACAATTCCAGAGGACATCTTAGGGAAAATAGCAGTTTCTATTGTAAAAGCATTAGA
ACATTTACATAGTAAGCTGTCTGTCATTCACAGAGACGTCAAGCCTTCTAATGTACTCAT
CAATGCTCTCGGTCAAGTGAAGATGTGCGATTTTGGAATCAGTGGCTACTTGGTGGACTC
TGTTGCTAAAACAATTGATGCAGGTTGCAAACCATACATGGCCCCTGAAAGAATAAACCC
AGAGCTCAACCAGAAGGGATACAGTGTGAAGTCTGACATTTGGAGTCTGGGCATCACGAT
GATTGAGTTGGCCATCCTTCGATTTCCCTATGATTCATGGGGAACTCCATTTCAGCAGCT
CAAACAGGTGGTAGAGGAGCCATCGCCACAACTCCCAGCAGACAAGTTCTCTGCAGAGTT
TGTTGACTTTACCTCACAGTGCTTAAAGAAGAATTCCAAAGAACGGCCTACATACCCAGA
GCTAATGCAACATCCATTTTTCACCCTACATGAATCCAAAGGAACAGATGTGGCATCTTT
TGTAAAACTGATTCTTGGAGACTAAAAAGCAGTGGACTTAATCGGTTGACCCTACTGTGG
ATTGGTGGGTTTCGGGGTGAAGCAAGTTCACTACAGCATCAATAGAAAGTCATCTTTGAG
ATAATTTAACCCTGCCTCTCAGAGGGTTTTCTCTCCCAATTTTCTTTTTACTCCCCCTCT
TAAGGGGGCCTTGGAATCTATAGTATAGAATGAACTGTCTAGATGGATGAATTATGATAA
AGGCTTAGGACTTCAAAAGGTGATTAAATATTTAATGATGTGTCATATGAGTCCTCAAGC
TTCTCAGACTTCTCTTATTCTTTACAAAATGAATGCATTGGCCCTGACAAAAAGGTGCTA
CGGTAGTGATGAAATTATAAGTAGATTTGTAGTTTGTCCCATTTATTATTTTAATATTTA
TGTTTAAGTGCTTGGTTGAAAAGATTCCATTTTATACAAGAAGGGAGATTCAAAAAAAAA
ATATAAGGTTGGGTTAGCAATATTTATAGGGCTTTTATTTTTTAAGTTCAATTGTGTCTG
TGGTCCAGAAGAAATTATTTAATATGCATCTTTGAGAATATTATAAAAATATCAAAAAGG
AGCTCTTCTTGTGAAATGTCTGTTCCAGCTGTTGTGACTGCTGCCATTTTTGGAAACATC
TGCCCAATCCTGGGTGATCACCACATCTTTTAGGGGAAGTGACAAGATGCTCTGGTCATA
CTCTTTTTCCCAACTTTGGAAAACATAAAAATCACTCATATAACAGCTCAAAGAGTAAAA
CATTTGGTTCTTCTGACACTTGTGGTATAGTATTAGTGGAAAGTGATTTGTAATATGATT
TTATATCCACCTACCTATTCATCTACCTGTGTGTATGTGTGTGTTTGTGTGTCTATTTGG
CAATTCACAAGTCCTGCCAAGTGGTTTCTATGAGCATCTCTGTTTGGTAAGGAGGACAAT
TGTCAGTTTTGAGGGGGACATGTGTTAAATCACAGAAAAAAATGGTGCCTTCTTCTGCGT
TTGTCCCTCCTGCCATGTGTAAGTTGTAAGGATTGCCTTTGTAGTTAATGTACTCTTTGG
CTTTGTTTGTTTGTTTTCTTCTTCAGTGAAGCAGCCTTACTATTCATAGAAGGGCTAGAA
TAGGAGAAAATGAAAGGTAGTGAGTAATTCTTTGATAAGATGAGGAAATAATGGGAAAGG
TTGAATTAATTCCTGGGCATGGACTACCAGATGACCACAAGTTGCGTTGAGGCCGCATCT
TTCTTCAGCAGCGTGCAATAGCTGGCTCCTCTATAGGAGATGAGCTTCATTGGGAGTTCC
TAGCAAGTTGACTAAACAGCAAAAGTTCTTTCTCGTGGGTAAATATACCCACAGGTTCTA
TGATTTGTAGCTCTAGGTTTCTTGATGATCAAGGAGTGAAGTAATTGACAGGGAAAATAT
AGACCTATGATAAATAACCAGGAAGCATTGCTTTTGGACAAGGAAGAACAGAGGGTTTTG
ATTTTAAAAAGAAGAAAAAAAAACCTTATTTTTTCTTTCTTGGCCTCAAGTTCAATATGG
AGAGGATTGCTTCCCTGAATCCTCTCTTCCTTCCCCTTTTAGAG

Protein sequence predicted based on nucleotide sequence

MSQSKGKKRNPGLKIPKEAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIMELG
RGAYGVVEKMRHVPSGQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCPFTVTFYGALF
REGDVWICMELMDTSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDV
KPSNVLINALGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDI
WSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSK
ERPTYPELMQHPFFTLHESKGTDVASFVKLILGD*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: chloramphenicol

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLDNT7_nFLAG

Synonyms:

pLDNT7-Flag

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning

Comments:

None

Size (bp):

4557

Parent Vector:

None

Properties:

Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker

Author Name:

Daniel Schiwek

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGCCGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAGAGATCTATAACTTCGTATAGCATACATTATACGAAGTTATTGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTGGACCCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE1 ori

ColE1 origin of replication

1842

2524

promoter

T7 promoter

4462

4479

recombination site

LoxP

LoxP site for recombinational cloning

657

690

selectable marker

AmpR

ampicillin resistance gene

2622

3281

ssDNA origin

f1 ori

f1 origin for ssDNA production

3928

4368

tag

Flag

3xFlag epitope tag ORF and LoxP region

585

650

trxn termination sequence

T7 term

T7 terminator sequence

1614

1660

Gene

Gene Symbol :

MAP2K6

Symbol Nomenclature :

MAP2K6

SYNONYM :

MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3; SAPKK3

Designation :

Full Nomenclature :

mitogen-activated protein kinase kinase 6

GENEID :

5608

HGNC :

6846

MIM :

601254

Target GenBank:

NM_002758

Pathways

NCI :

CDC42 signaling events

NCI :

Cellular roles of Anthrax toxin

NCI :

CXCR3-mediated signaling events

NCI :

FAS (CD95) signaling pathway

NCI :

IL1-mediated signaling events

NCI :

IL12-mediated signaling events

NCI :

IL6-mediated signaling events

NCI :

p38 MAPK signaling pathway

NCI :

RAC1 signaling pathway

NCI :

Regulation of Androgen receptor activity

NCI :

Regulation of p38-alpha and p38-beta

NCI :

RhoA signaling pathway

NCI :

Signaling events mediated by VEGFR1 and VEGFR2

NCI :

Signaling mediated by p38-gamma and p38-delta

NCI :

Trk receptor signaling mediated by the MAPK pathway

Panther :

EGF receptor signaling pathway

Panther :

FGF signaling pathway

Panther :

Oxidative stress response

Panther :

p38 MAPK pathway

Panther :

Ras Pathway

Reactome :

activated TAK1 mediates p38 MAPK activation

Reactome :

Activated TLR4 signalling

Reactome :

CDO in myogenesis

Reactome :

Cellular responses to stress

Reactome :

Cellular Senescence

Reactome :

Cytokine Signaling in Immune system

Reactome :

Developmental Biology

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

Interleukin-1 signaling

Reactome :

MAP kinase activation in TLR cascade

Reactome :

MyD88 cascade initiated on plasma membrane

Reactome :

MyD88 dependent cascade initiated on endosome

Reactome :

MyD88-independent cascade

Reactome :

MyD88:Mal cascade initiated on plasma membrane

Reactome :

Myogenesis

Reactome :

NOD1/2 Signaling Pathway

Reactome :

Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways

Reactome :

Oxidative Stress Induced Senescence

Reactome :

Signaling by Interleukins

Reactome :

Toll Like Receptor 10 (TLR10) Cascade

Reactome :

Toll Like Receptor 2 (TLR2) Cascade

Reactome :

Toll Like Receptor 3 (TLR3) Cascade

Reactome :

Toll Like Receptor 4 (TLR4) Cascade

Reactome :

Toll Like Receptor 5 (TLR5) Cascade

Reactome :

Toll Like Receptor 7/8 (TLR7/8) Cascade

Reactome :

Toll Like Receptor 9 (TLR9) Cascade

Reactome :

Toll Like Receptor TLR1:TLR2 Cascade

Reactome :

Toll Like Receptor TLR6:TLR2 Cascade

Reactome :

Toll-Like Receptors Cascades

Reactome :

TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation

Reactome :

TRAF6 Mediated Induction of proinflammatory cytokines

Reactome :

TRIF-mediated TLR3/TLR4 signaling

WikiPathway :

angiogenesis overview

WikiPathway :

B Cell Receptor Signaling Pathway

WikiPathway :

Focal Adhesion

WikiPathway :

IL-1 signaling pathway

WikiPathway :

Insulin Signaling

WikiPathway :

Integrin-mediated cell adhesion

WikiPathway :

MAPK Cascade

WikiPathway :

MAPK signaling pathway

WikiPathway :

MicroRNAs in cardiomyocyte hypertrophy

WikiPathway :

p38 MAPK Signaling Pathway

WikiPathway :

RANKL/RANK Signaling Pathway

WikiPathway :

Regulation of toll-like receptor signaling pathway

WikiPathway :

Serotonin HTR1 Group and FOS Pathway

WikiPathway :

TGF beta Signaling Pathway

WikiPathway :

TNF alpha Signaling Pathway

WikiPathway :

Toll-like receptor signaling pathway

WikiPathway :

TSH signaling pathway

Protein

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

UniProt :

A8K3Y2

UniProt :

P52564

HPRD :

03155

Clone

Cloning Information :

654904

HIP Master Clone ID :

33458

Original Clone ID :

FLH230874.01X

Original Clone ID :

HsCD00298726

Organism

Species Specific ID:

5608

Genome

Chromosome :

Map Location :

17q24.3

Reagents

Labome :

MKK6-antibody

MAP2K6 (H. sapiens )In pLDNT7_nFLAG (Flag-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Organism

Genome

Reagents

MAP2K6 (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)