Gene:
Gene Symbol:
ERN1
Gene Name:
endoplasmic reticulum to nucleus signaling 1
Original Clone ID:
HsCD00298655
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618082
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2934nts
Open reading frame: 97 to 3030
CCGGCTCGACGGCTCGGTCACCGCCTCGCTGTCGTCGCGGCGCCCCCGGCCGTCCTCTGT
CCGTACCGCCCCCGGAGCCAGGGCCGAGTCCTCGCCATGCCGGCCCGGCGGCTGCTGCTG
CTGCTGACGCTGCTGCTGCCCGGCCTCGGGATTTTTGGAAGTACCAGCACAGTGACGCTT
CCTGAAACCTTGTTGTTTGTGTCAACGCTGGATGGAAGTTTGCATGCTGTCAGCAAGAGG
ACAGGCTCAATCAAATGGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACACATGTG
GAAGAGCCTGCCTTTCTCCCAGATCCTAATGATGGCAGCCTGTATACGCTTGGAAGCAAG
AATAATGAAGGCCTGACGAAACTTCCTTTTACCATCCCAGAATTGGTGCAGGCATCCCCA
TGCCGAAGTTCAGATGGAATCCTCTACATGGGTAAAAAGCAGGACATCTGGTATGTTATT
GACCTCCTGACCGGAGAGAAGCAGCAGACTTTGTCATCGGCCTTTGCAGATAGTCTCTGC
CCATCAACCTCTCTTCTGTATCTTGGGCGAACAGAATACACCATCACCATGTACGACACC
AAAACCCGAGAGCTCCGGTGGAATGCCACCTACTTTGACTATGCGGCCTCACTGCCTGAG
GACGAAGGGGACTACAAGATGTCCCACTTTGTGTCCAATGGTGATGGGCTGGTGGTGACT
GTGGACAGTGAATCTGGGGACGTCCTGTGGATCCAAAACTACGCCTCCCCTGTGGTGGCC
TTTTATGTCTGGCAGCGGGAGGGTCTGAGGAAGGTGATGCACATCAATGTCGCTGTGGAG
ACCCTGCGCTATCTGACCTTCATGTCTGGGGAGGTGGGGCGCATCACAAAGTGGAAGTAC
CCGTTCCCCAAGGAGACAGAGGCCAAGAGCAAGCTGACGCCCACTCTGTATGTTGGGAAA
TACTCTACCAGCCTCTATGCCTCTCCCTCAATGGTACACGAGGGGGTTGCTGTCGTGCCC
CGCGGCAGCACACTTCCTTTGCTGGAAGGGCCCCAGACTGATGGCGTCACCATCGGGGAC
AAGGGGGAGTGTGTGATCACGCCCAGCACGGACGTCAAGTTTGATCCCGGACTCAAAAGC
AAGAACAAGCTCAACTACTTGAGGAATTACTGGCTTCTGATAGGACACCATGAAACCCCA
CTGTCTGCGTCTACCAAGATGCTGGAGAGATTTCCCAACAATCTACCCAAACATCGGGAA
AATGTGATTCCTGCTGATTCAGAGAAAAAGAGCTTTGAGGAAGTTATCAACCTGGTTGAC
CAGACTTCAGAAAACGCACCTACCACCGTGTCTCGGGATGTGGAGGAGAAGCCCGCCCAT
GCCCCTGCCCGGCCCGAGGCCCCCGTGGACTCCATGCTTAAGGACATGGCTACCATCATC
CTGAGCACCTTCCTGCTGATTGGCTGGGTGGCCTTCATCATCACCTATCCCCTGAGCATG
CATCAGCAGCAGCAGCTCCAGCACCAGCAGTTCCAGAAGGAACTGGAGAAGATCCAGCTC
CTGCAGCAGCAGCAGCAGCAGCTGCCCTTCCACCCACCTGGAGACACGGCTCAGGACGGC
GAGCTCCTGGACACGTCTGGCCCGTACTCAGAGAGCTCGGGCACCAGCAGCCCCAGCACG
TCCCCCAGGGCCTCCAACCACTCGCTCTGCTCCGGCAGCTCTGCCTCCAAGGCTGGCAGC
AGCCCCTCCCTGGAACAAGACGATGGAGATGAGGAAACCAGCGTGGTGATAGTTGGGAAA
ATTTCCTTCTGTCCCAAGGATGTCCTGGGCCATGGAGCTGAGGGCACAATTGTGTACCGG
GGCATGTTTGACAACCGCGACGTGGCCGTGAAGAGGATCCTCCCCGAGTGTTTTAGCTTC
GCAGACCGTGAGGTCCAGCTGTTGCGAGAATCGGATGAGCACCCGAACGTGATCCGCTAC
TTCTGCACGGAGAAGGACCGGCAATTCCAGTACATTGCCATCGAGCTGTGTGCAGCCACC
CTGCAAGAGTATGTGGAGCAGAAGGACTTTGCGCATCTCGGCCTGGAGCCCATCACCTTG
CTGCAGCAGACCACCTCGGGCCTGGCCCACCTCCACTCCCTCAACATCGTTCACAGAGAC
CTAAAGCCACACAACATCCTCATATCCATGCCCAATGCACACGGCAAGATCAAGGCCATG
ATCTCCGACTTTGGCCTCTGCAAGAAGCTGGCAGTGGGCAGACACAGTTTCAGCCGCCGA
TCTGGGGTGCCTGGCACAGAAGGCTGGATCGCTCCAGAGATGCTGAGCGAAGACTGTAAG
GAGAACCCTACCTACACGGTGGACATCTTTTCTGCAGGCTGCGTCTTTTACTACGTGGTC
TCTGAGGGCAGCCACCCTTTTGGCAAGTCCCTGCAGCGGCAGGCCAACATCCTCCTGGGT
GCCTGCAGCCTTGACTGCTTGCACCCAGAGAAGCACGAAGACGTCATTGCACGAGAATTG
ATAGAGAAGATGATTGCGATGGATCCTCAGAAACGCCCCTCAGCGAACGACGTGCTCAAA
CACCCGTTCTTCTGGAGCCTAGAGAAGCAGCTCCAGTTCTTCCAGGACGTGAGCGACAGA
ATAGAAAAGGAATCCCTGGATGGCCCGATCGTGAAGCAGTTAGAGAGAGGCGGGAGAGCC
GTGGTGAAGATGGACTGGCGGGAGAACATCACTGACCCCCTCCAGACAGACCTGCGTAAA
TTCAGGACCTATAAAGGTGGTTCTGTCAGAGATCTCCTCCGAGCCATGAGAAATAAGAAG
CACCACTACCGGGAGCTGCCTGCAGAGGTGCGGGAGACGCTGGGGACCCTCCCCGACGAC
TTCGTGTGCTACTTCACGTCTCGCTTCCCCCACCTCCTCGCACACACCTACCGGGCCATG
GAGCTGTGCAGCCACGAGAGACTCTTCCAGCCCTACTACTTCCACGAGCCCCCAGAGCCC
CAGCCCCCAGTGACTCCAGACGCCCTCTGAGCGAGGGCGGCCCCTCTGTTCTGGTGGCCC
CAGCTGTGACTGAGGGCCTGGTCACCACAATTAGAGCTTGATGCCTCCCGGCTTTGCAGG
GAGACCAGGCTTCCCAAACCAAGTGCCTTGAGCTGCCTGCTCTGCAGCCCACAGAGGACA
GTGCTGACCCCAGGAAGTGGGAGAAGTGGCCCCTCGTGACCTACAGGGAACTGGGAAGAT
GCTGGCCCCAAAAGCCTTACGGTCATGATGTCTGCAAAGGAGGGCCTCAGAGACAGCGCG
AGTAGCACCCCCAGCCATCTACTGGATAAACTTGCTTCAGACTTTTTAAATTCCTGCTTA
ATGTCAGTCTACAGGCCTTTCAGGAAGGGAGAGGAGGGAATCGTACATTTTGCTTGCGTG
CTGGGACAGCTAGGCTGAGATGCACCAAGTACAGCCTTCACTGGAGACCGGAATTGAGAG
GTGGGGGATGCTGAGGAGGGGGAGGACGGAGTTCAGAGGGTGTCGTCCTGCAGTATGAGA
TTTCTCATTGATCACAGATGTGCCCAGAGTAGCCCAGGTCACTGTTAACTAGTGTTTCTG
CAGAGGCAGCAGGAGCCAGCCCGGAATTC
Protein sequence predicted based on nucleotide sequence
MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDP VLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGK KQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYF DYAASLPEDEGDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKV MHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMV HEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWL LIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSR DVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQ KELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSG SSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKR ILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAH LGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAV GRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVVSEGSHPFGKSLQ RQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSANDVLKHPFFWSLEKQLQ FFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITDPLQTDLRKFRTYKGGSVRDL LRAMRNKKHHYRELPAEVRETLGTLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPY YFHEPPEPQPPVTPDAL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
