DNASU Plasmid | CDC7 (H. sapiens )<br>In pLDNT7_nFLAG (Flag-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: CDC7

Gene Name: cell division cycle 7

Original Clone ID:

HsCD00301051

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLDNT7_nFLAG

Format:

CLOSED

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00618087

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1725nts

Open reading frame: 126 to 1850

GATCTCTTGGAGACGGCGACCCAGGCATCTGGGGAGCCACAGAAGTCGTACTCCCTTAAA
CCCTGCTTTGCTCCCCCTGTGGATGTAACCCCTTAGCTGGCATTTTGCATCTCAATTGGC
TTGTGATGGAGGCGTCTTTGGGGATTCAGATGGATGAGCCAATGGCTTTTTCTCCCCAGC
GTGACCGGTTTCAGGCTGAAGGCTCTTTAAAAAAAAACGAGCAGAATTTTAAACTTGCAG
GTGTTAAAAAAGATATTGAGAAGCTTTATGAAGCTGTACCACAGCTTAGTAATGTGTTTA
AGATTGAGGACAAAATTGGAGAAGGCACTTTCAGCTCTGTTTATTTGGCCACAGCACAGT
TACAAGTAGGACCTGAAGAGAAAATTGCTCTAAAACACTTGATTCCAACAAGTCATCCTA
TAAGAATTGCAGCTGAACTTCAGTGCCTAACAGTGGCTGGGGGGCAAGATAATGTCATGG
GAGTTAAATACTGCTTTAGGAAGAATGATCATGTAGTTATTGCTATGCCATATCTGGAGC
ATGAGTCGTTTTTGGACATTCTGAATTCTCTTTCCTTTCAAGAAGTACGGGAATATATGC
TTAATCTGTTCAAAGCTTTGAAACGCATTCATCAGTTTGGTATTGTTCACCGTGATGTTA
AGCCCAGCAATTTTTTATATAATAGGCGCCTGAAAAAGTATGCCTTGGTAGACTTTGGTT
TGGCCCAAGGAACCCATGATACGAAAATAGAGCTTCTTAAATTTGTCCAGTCTGAAGCTC
AGCAGGAAAGGTGTTCACAAAACAAATCCCACATAATCACAGGAAACAAGATTCCACTGA
GTGGCCCAGTACCTAAGGAGCTGGATCAGCAGTCCACCACAAAAGCTTCTGTTAAAAGAC
CCTACACAAATGCACAAATTCAGATTAAACAAGGAAAAGACGGAAAGGAGGGATCTGTAG
GCCTTTCTGTCCAGCGCTCTGTTTTTGGAGAAAGAAATTTCAATATACACAGCTCCATTT
CACATGAGAGCCCTGCAGTGAAACTCATGAAGCAGTCAAAGACTGTGGATGTACTGTCTA
GAAAGTTAGCAACAAAAAAGAAGGCTATTTCTACGAAAGTTATGAATAGTGCTGTGATGA
GGAAAACTGCCAGTTCTTGCCCAGCTAGCCTGACCTGTGACTGCTATGCAACAGATAAAG
TTTGTAGTATTTGCCTTTCAAGGCGTCAGCAGGTTGCCCCTAGGGCAGGTACACCAGGAT
TCAGAGCACCAGAGGTCTTGACAAAGTGCCCCAATCAAACTACAGCAATTGACATGTGGT
CTGCAGGTGTCATATTTCTTTCTTTGCTTAGTGGACGATATCCATTTTATAAAGCAAGTG
ATGATTTAACTGCTTTGGCCCAAATTATGACAATTAGGGGATCCAGAGAAACTATCCAAG
CTGCTAAAACTTTTGGGAAATCAATATTATGTAGCAAAGAAGTTCCAGCACAAGACTTGA
GAAAACTCTGTGAGAGACTCAGGGGTATGGATTCTAGCACTCCCAAGTTAACAAGTGATA
TACAAGGGCATGCTTCTCATCAACCAGCTATTTCAGAGAAGACTGACCATAAAGCTTCTT
GCCTCGTTCAAACACCTCCAGGACAATACTCAGGGAATTCATTTAAAAAGGGGGATAGTA
ATAGCTGTGAGCATTGTTTTGATGAGTATAATACCAATTTAGAAGGCTGGAATGAGGTAC
CTGATGAAGCTTATGACCTGCTTGATAAACTTCTAGATCTAAATCCAGCTTCAAGAATAA
CAGCAGAAGAAGCTTTGTTGCATCCATTTTTTAAAGATATGAGCTTGTGATAATGGATCT
TCATTTAATGTTTACTGTTATGAGGTAGAATAAAAAAGAATACTTTGTAATAGCCACAAG
TTCTTGTTTAGAGACCAGAGCAGGATTAATAATTTATTTTAACATTTTAGTGTTTGGTGG
CACATTCTAAAATATAGATTAAGAATACTTAAAATGCCTGGGATAGTTCTTGGGACTAAC
AACATGATCTTCTTTGAGTTAAACCTACCTAAGTAGATTTTAGGTGGGTTCCTATTAGGT
CAGATTTTTAGCTTCCCTAATTACCTTTCACTGACATACAGAAAAAGGAGCAGTTTTAGT
TTTAATTAATTAAAATTAACAGATGTGATGAGGATTAAATGAATCAAAAGACTTAATTTG
TAGATTCTTTTAGAGTTATGAGCTAGGTATAGTTTGGGGAAACTCAACCTGGTGCTGGTG
CTCTTAACAATTTTGTAAATAAAGAAGATAATTTCCTTTTCTAGAGGTACATATTAGGCC
TTTTATGAACACTAAAACAATGAGGAAATGTTGGTCATGGGGCAAAGTATCACTTAAAAT
TGAATTCATCCATTTTTAAAAAACACTTCATGAAAGCATTCTGGTGTGAATTGCCATTTT
TTTCTTACTGGCTTCTCAATTTTCTTCCTTCTCTGCCCCTACCTAAAACATTCTCCTCGG
AAATTACATGGTGCTGACCACAAAGTTTCTGGATGTTTTATTAAATATTGTACGTGTTTA
CAGTTGGGAATTTAAAATAATACATACACTGGTTGATAAAGGGAAGCTGCAGGACCAAGG
TGAAGATTGATAGTCCAAATGCTTTTCTTTTTTGAGTTGTATATTTTTTCACACCATCTT
AGATATAATTAGGTAGCTGCTGAAAGGAAAAGTGAATACAGAATTGACGGTATTATTGGA
GATTTTTCCTCTGCGTAGAGCCATCCAGATCTCTGTATCCTGTTTTGACTAAGTCTTAGG
TGGGTTGGGAAGACAGATAATGAAGTAGGCAAAGAGAAAAGGACCCAAGATAGAGGTTTA
TATTCAGAAATGGTATATATCAATGACAGCATATCAAACTTCCTATGGGAAAAAGTCTGG
TGGGTGGTCAGCTGACAGATTTCCCATTTAGTAGTCATAGAATACAGAAATAGTTTAGGG
ACATGTATTCATTTTGTTATTTTGAGCATTGATAGGTCAGTATATCTACCTAATCTGTTT
GGTAAGTATAGGATATATAAACCATTACCATTGATCTGTCTTATGCCATAATCTTAAAAA
AAAATTGAATGCTCTTGAATTTGTATATTCAATAAAGTTATCCTTTTATAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MEASLGIQMDEPMAFSPQRDRFQAEGSLKKNEQNFKLAGVKKDIEKLYEAVPQLSNVFKI
EDKIGEGTFSSVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQDNVMGV
KYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREYMLNLFKALKRIHQFGIVHRDVKP
SNFLYNRRLKKYALVDFGLAQGTHDTKIELLKFVQSEAQQERCSQNKSHIITGNKIPLSG
PVPKELDQQSTTKASVKRPYTNAQIQIKQGKDGKEGSVGLSVQRSVFGERNFNIHSSISH
ESPAVKLMKQSKTVDVLSRKLATKKKAISTKVMNSAVMRKTASSCPASLTCDCYATDKVC
SICLSRRQQVAPRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFYKASDD
LTALAQIMTIRGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLRGMDSSTPKLTSDIQ
GHASHQPAISEKTDHKASCLVQTPPGQYSGNSFKKGDSNSCEHCFDEYNTNLEGWNEVPD
EAYDLLDKLLDLNPASRITAEEALLHPFFKDMSL*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: chloramphenicol

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLDNT7_nFLAG

Synonyms:

pLDNT7-Flag

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning

Comments:

None

Size (bp):

4557

Parent Vector:

None

Properties:

Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker

Author Name:

Daniel Schiwek

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGCCGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAGAGATCTATAACTTCGTATAGCATACATTATACGAAGTTATTGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTGGACCCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE1 ori

ColE1 origin of replication

1842

2524

promoter

T7 promoter

4462

4479

recombination site

LoxP

LoxP site for recombinational cloning

657

690

selectable marker

AmpR

ampicillin resistance gene

2622

3281

ssDNA origin

f1 ori

f1 origin for ssDNA production

3928

4368

tag

Flag

3xFlag epitope tag ORF and LoxP region

585

650

trxn termination sequence

T7 term

T7 terminator sequence

1614

1660

Gene

Gene Symbol :

CDC7

Symbol Nomenclature :

CDC7

SYNONYM :

CDC7L1; HsCDC7; Hsk1; huCDC7

Designation :

CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1|CDC7-related kinase|cell division cycle 7 homolog|cell division cycle 7-like protein 1|cell division cycle 7-related protein kinase

Full Nomenclature :

cell division cycle 7

GENEID :

8317

HGNC :

1745

MIM :

603311

Vega :

OTTHUMG00000047810

Target GenBank:

NM_003503

Pathways

Reactome :

Activation of ATR in response to replication stress

Reactome :

Activation of the pre-replicative complex

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

DNA Replication

Reactome :

DNA Replication Pre-Initiation

Reactome :

G1/S Transition

Reactome :

G2/M Checkpoints

Reactome :

M/G1 Transition

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic M-M/G1 phases

WikiPathway :

Cell cycle

WikiPathway :

DNA Replication

Protein

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

UniProt :

O00311

HPRD :

10345

Clone

Cloning Information :

649634

HIP Master Clone ID :

148002

Original Clone ID :

FLH232188.01L

Original Clone ID :

HsCD00301051

Organism

Species Specific ID:

8317

Genome

Chromosome :

Map Location :

1p22

Ensembl :

ENSG00000097046

Reagents

Labome :

CDC7-antibody

CDC7 (H. sapiens )In pLDNT7_nFLAG (Flag-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Pathways

Protein

Clone

Organism

Genome

Reagents

CDC7 (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)