Gene:
Gene Symbol:
YES1
Gene Name:
v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Original Clone ID:
HsCD00299223
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618090
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1632nts
Open reading frame: 208 to 1839
GCGGAGCCAAGGCACACGGGTCTGACCCTTGGGCCGGCCCGGAGCAAGTGACACGGACCG
GTCGCCTATCCTGACCACAGCAAAGCGGCCCGGAGCCCGCGGAGGGGACCTGACGGGGGC
GTAGGCGCCGGAAGGCTGGGGGCCCCGGAGCCGGGCCGGCGTGGCCCGAGTTCCGGTGAG
CGGACGGCGGCGCGCGCAGATTTGATAATGGGCTGCATTAAAAGTAAAGAAAACAAAAGT
CCAGCCATTAAATACAGACCTGAAAATACTCCAGAGCCTGTCAGTACAAGTGTGAGCCAT
TATGGAGCAGAACCCACTACAGTGTCACCATGTCCGTCATCTTCAGCAAAGGGAACAGCA
GTTAATTTCAGCAGTCTTTCCATGACACCATTTGGAGGATCCTCAGGGGTAACGCCTTTT
GGAGGTGCATCTTCCTCATTTTCAGTGGTGCCAAGTTCATATCCTGCTGGTTTAACAGGT
GGTGTTACTATATTTGTGGCCTTATATGATTATGAAGCTAGAACTACAGAAGACCTTTCA
TTTAAGAAGGGTGAAAGATTTCAAATAATTAACAATACGGAAGGAGATTGGTGGGAAGCA
AGATCAATCGCTACAGGAAAGAATGGTTATATCCCGAGCAATTATGTAGCGCCTGCAGAT
TCCATTCAGGCAGAAGAATGGTATTTTGGCAAAATGGGGAGAAAAGATGCTGAAAGATTA
CTTTTGAATCCTGGAAATCAACGAGGTATTTTCTTAGTAAGAGAGAGTGAAACAACTAAA
GGTGCTTATTCCCTTTCTATTCGTGATTGGGATGAGATAAGGGGTGACAATGTGAAACAC
TACAAAATTAGGAAACTTGACAATGGTGGATACTATATCACAACCAGAGCACAATTTGAT
ACTCTGCAGAAATTGGTGAAACACTACACAGAACATGCTGATGGTTTATGCCACAAGTTG
ACAACTGTGTGTCCAACTGTGAAACCTCAGACTCAAGGTCTAGCAAAAGATGCTTGGGAA
ATCCCTCGAGAATCTTTGCGACTAGAGGTTAAACTAGGACAAGGATGTTTCGGCGAAGTG
TGGATGGGAACATGGAATGGAACCACGAAAGTAGCAATCAAAACACTAAAACCAGGTACA
ATGATGCCAGAAGCTTTCCTTCAAGAAGCTCAGATAATGAAAAAATTAAGACATGATAAA
CTTGTTCCACTATATGCTGTTGTTTCTGAAGAACCAATTTACATTGTCACTGAATTTATG
TCAAAAGGAAGCTTATTAGATTTCCTTAAGGAAGGAGATGGAAAGTATTTGAAGCTTCCA
CAGCTGGTTGATATGGCTGCTCAGATTGCTGATGGTATGGCATATATTGAAAGAATGAAC
TATATTCACCGAGATCTTCGGGCTGCTAATATTCTTGTAGGAGAAAATCTTGTGTGCAAA
ATAGCAGACTTTGGTTTAGCAAGGTTAATTGAAGACAATGAATACACAGCAAGACAAGGT
GCAAAATTTCCAATCAAATGGACAGCTCCTGAAGCTGCACTGTATGGTCGGTTTACAATA
AAGTCTGATGTCTGGTCATTTGGAATTCTGCAAACAGAACTAGTAACAAAGGGCCGAGTG
CCATATCCAGGTATGGTGAACCGTGAAGTACTAGAACAAGTGGAGCGAGGATACAGGATG
CCGTGCCCTCAGGGCTGTCCAGAATCCCTCCATGAATTGATGAATCTGTGTTGGAAGAAG
GACCCTGATGAAAGACCAACATTTGAATATATTCAGTCCTTCTTGGAAGACTACTTCACT
GCTACAGAGCCACAGTACCAGCCAGGAGAAAATTTATAATTCAAGTAGCCTATTTTATAT
GCACAAATCTGCCAAAATATAAAGAACTTGTGTAGATTTTCTACAGGAATCAAAAGAAGA
AAATCTTCTTTACTCTGCATGTTTTTAATGGTAAACTGGAATCCCAGATATGGTTGCACA
AAACCACTTTTTTTTCCCCAAGTATTAAACTCTAATGTACCAATGATGAATTTATCAGCG
TATTTCAGGGTCCAAACAAAATAGAGCTAAGATACTGATGACAGTGTGGGTGACAGCATG
GTAATGAAGGACAGTGAGGCTCCTGCTTATTTATAAATCATTTCCTTTCTTTTTTTCCCC
AAAGTCAGAATTGCTCAAAGAAAATTATTTATTGTTACAGATAAAACTTGAGAGATAAAA
AGCTATACCATAATAAAATCTAAAATTAAGGAATATCATGGGACCAAATAATTCCATTCC
AGTTTTTTAAAGTTTCTTGCATTTATTATTCTCAAAAGTTTTTTCTAAGTTAAACAGTCA
GTATGCAATCTTAATATATGCTTTCTTTTGCATGGACATGGGCCAGGTTTTTCAAAAGGA
ATATAAACAGGATCTCAAACTTGATTAAATGTTAGACCACAGAAGTGGAATTTGAAAGTA
TAATGCAGTACATTAATATTCATGTTCATGGAACTGAAAGAATAAGAACTTTTTCACTTC
AGTCCTTTTCTGAAGAGTTTGACTTAGAATAATGAAGGTAACTAGAAAGTGAGTTAATCT
TGTATGAGGTTGCATTGATTTTTTAAGGCAATATATAATTGAAACTACTGTCCAATCAAA
GGGGAAATGTTTTGATCTTTAGATAGCATGCAAAGTAAGACCCAGCATTTTAAAAGCCCT
TTTTTAAAAACTAGACTTCGTACTGTGAGTATTGCTTATATGTCCTTATGGGGATGGGTG
CCACAAATAGAAAATATGACCAGATCAGGGACTTGAATGCACTTTTGCTCATGGTGAATA
TAGATGAACAGAGAGGAAAATGTATTTAAAAGAAATACGAGAAAAGAAAATGTGAAAGTT
TTACAAGTTAGAGGGATGGAAGGTAATGTTTAATGTTGATGTCATGGAGTGACAGAATGG
CTTTGCTGGCACTCAGAGCTCCTCACTTAGCTATATTCTGAGACTTTGAAGAGTTATAAA
GTATAACTATAAAACTAATTTTTCTTACACACTAAATGGGTATTTGTTCAAAATAATGAA
GTTATGGCTTCACATTCATTGCAGTGGGATATGGTTTTTATGTAAAACATTTTTAGAACT
CCAGTTTTCAAATCATGTTTGAATCTACATTCACTTTTTTTTGTTTTCTTTTTTGAGACG
GAGTCTCGCTCTGCCGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT
CTGCCTCCCAGGTTCACACCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGG
TGCCCACCACCACGCCTGGCTAGTTTTTTGTATTTTTAGTAGAGACGCAGTTTCACCGTG
TTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCTGCCCGCCTCGGCCTCCCAAAGT
GCTGGGATTACAGGTGTGAGCCACCGCGCCCAGCCTACATTCACTTCTAAAGTCTATGTA
ATGGTGGTCATTTTTTCCCTTTTAGAATACATTAAATGGTTGATTTGGGGAGGAAAACTT
ATTCTGAATATTAACGGTGGTGAAAAGGGGACAGTTTTTACCCTAAAGTGCAAAAGTGAA
ACATACAAAATAAGACTAATTTTTAAGAGTAACTCAGTAATTTCAAAATACAGATTTGAA
TAGCAGCATTAGTGGTTTGAGTGTCTAGCAAAGGAAAAATTGATGAATAAAATGAAGGTC
TGGTGTATATGTTTTAAAATACTCTCATATAGTCACACTTTAAATTAAGCCTTATATTAG
GCCCCTCTATTTTCAGGATATAATTCTTAACTATCATTATTTACCTGATTTTAATCATCA
GATTCGAAATTCTGTGCCATGGCGTATATGTTCAAATTCAAACCATTTTTAAAATGTGAA
GATGGACTTCATGCAAGTTGGCAGTGGTTCTGGTACTAAAAATTGTGGTTGTTTTTTCTG
TTTACGTAACCTGCTTAGTATTGACACTCTCTACCAAGAGGGTCTTCCTAAGAAGAGTGC
TGTCATTATTTCCTCTTATCAACAACTTGTGACATGAGATTTTTTAAGGGCTTTATGTGA
ACTATGATATTGTAATTTTTCTAAGCATATTCAAAAGGGTGACAAAATTACGTTTATGTA
CTAAATCTAATCAGGAAAGTAAGGCAGGAAAAGTTGATGGTATTCATTAGGTTTTAACTG
AATGGAGCAGTTCCTTATATAATAACAATTGTATAGTAGGGATAAAACACTAACAATGTG
TATTCATTTTAAATTGTTCTGTATTTTTAAATTGCCAAGAAAAACAACTTTGTAAATTTG
GAGATATTTTCCAACAGCTTTTCGTCTTCAGTGTCTTAATGTGGAAGTTAACCCTTACCA
AAAAAGGAAGTTGGCAAAAACAGCCTTCTAGCACACTTTTTTAAATGAATAATGGTAGCC
TAAACTTAATATTTTTATAAAGTATTGTAATATTGTTTTGTGGATAATTGAAATAAAAAG
TTCTCATTGAATGCACC
Protein sequence predicted based on nucleotide sequence
MGCIKSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLSMT PFGGSSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEARTTEDLSFKKGERFQI INNTEGDWWEARSIATGKNGYIPSNYVAPADSIQAEEWYFGKMGRKDAERLLLNPGNQRG IFLVRESETTKGAYSLSIRDWDEIRGDNVKHYKIRKLDNGGYYITTRAQFDTLQKLVKHY TEHADGLCHKLTTVCPTVKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTT KVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFL KEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIADFGLARL IEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTELVTKGRVPYPGMVNRE VLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDERPTFEYIQSFLEDYFTATEPQYQPG ENL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
