Gene:
Gene Symbol:
NCAM1
Gene Name:
neural cell adhesion molecule 1
Original Clone ID:
FLH232254.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618140
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2577nts
Open reading frame: 179 to 2755
GCGCGTGAACGCAGCTCGGCTGCCGCTGGCAGGAAACAATTCTGCAAAAATAATCATACT
CAGCCTGGCAATTGTCTGCCCCTAGGTCTGTCGCTCAGCCGCCGTCCACACTCGCTGCAG
GGGGGGGGGGCACAGAATTTACCGCGGCAAGAACATCCCTCCCAGCCAGCAGATTACAAT
GCTGCAAACTAAGGATCTCATCTGGACTTTGTTTTTCCTGGGAACTGCAGTTTCTCTGCA
GGTGGATATTGTTCCCAGCCAGGGGGAGATCAGCGTTGGAGAGTCCAAATTCTTCTTATG
CCAAGTGGCAGGAGATGCCAAAGATAAAGACATCTCCTGGTTCTCCCCCAATGGAGAAAA
GCTCACCCCAAACCAGCAGCGGATCTCAGTGGTGTGGAATGATGATTCCTCCTCCACCCT
CACCATCTATAACGCCAACATCGACGACGCCGGCATTTACAAGTGTGTGGTTACAGGCGA
GGATGGCAGTGAGTCAGAGGCCACCGTCAACGTGAAGATCTTTCAGAAGCTCATGTTCAA
GAATGCGCCAACCCCACAGGAGTTCCGGGAGGGGGAAGATGCCGTGATTGTGTGTGATGT
GGTCAGCTCCCTCCCACCAACCATCATCTGGAAACACAAAGGCCGAGATGTCATCCTGAA
AAAAGATGTCCGATTCATAGTCCTGTCCAACAACTACCTGCAGATCCGGGGCATCAAGAA
AACAGATGAGGGCACTTATCGCTGTGAGGGCAGAATCCTGGCACGGGGGGAGATCAACTT
CAAGGACATTCAGGTCATTGTGAATGTGCCACCTACCATCCAGGCCAGGCAGAATATTGT
GAATGCCACCGCCAACCTCGGCCAGTCCGTCACCCTGGTGTGCGATGCCGAAGGCTTCCC
AGAGCCCACCATGAGCTGGACAAAGGATGGGGAACAGATAGAGCAAGAGGAAGACGATGA
GAAGTACATCTTCAGCGACGATAGTTCCCAGCTGACCATCAAAAAGGTGGATAAGAACGA
CGAGGCTGAGTACATCTGCATTGCTGAGAACAAGGCTGGCGAGCAGGATGCGACCATCCA
CCTCAAAGTCTTTGCAAAACCCAAAATCACATATGTAGAGAACCAGACTGCCATGGAATT
AGAGGAGCAGGTCACTCTTACCTGTGAAGCCTCCGGAGACCCCATTCCCTCCATCACCTG
GAGGACTTCTACCCGGAACATCAGCAGCGAAGAAAAGGCTTCGTGGACTCGACCAGAGAA
GCAAGAGACTCTGGATGGGCACATGGTGGTGCGTAGCCATGCCCGTGTGTCGTCGCTGAC
CCTGAAGAGCATCCAGTACACTGATGCCGGAGAGTACATCTGCACCGCCAGCAACACCAT
CGGCCAGGACTCCCAGTCCATGTACCTTGAAGTGCAATATGCCCCAAAGCTACAGGGCCC
TGTGGCTGTGTACACTTGGGAGGGGAACCAGGTGAACATCACCTGCGAGGTATTTGCCTA
TCCCAGTGCCACGATCTCATGGTTTCGGGATGGCCAGCTGCTGCCAAGCTCCAATTACAG
CAATATCAAGATCTACAACACCCCCTCTGCCAGCTATCTGGAGGTGACCCCAGACTCTGA
GAATGATTTTGGGAACTACAACTGTACTGCAGTGAACCGCATTGGGCAGGAGTCCTTGGA
ATTCATCCTTGTTCAAGCAGACACCCCCTCTTCACCATCCATCGACCAGGTGGAGCCATA
CTCCAGCACAGCCCAGGTGCAGTTTGATGAACCAGAGGCCACAGGTGGGGTGCCCATCCT
CAAATACAAAGCTGAGTGGAGAGCAGTGGGTGAAGAAGTATGGCATTCCAAGTGGTATGA
TGCCAAGGAAGCCAGCATGGAGGGCATCGTCACCATCGTGGGCCTGAAGCCCGAAACAAC
GTACGCCGTAAGGCTGGCGGCGCTCAATGGCAAAGGGCTGGGTGAGATCAGCGCGGCCTC
CGAGTTCAAGACGCAGCCAGTCCAAGGGGAACCCAGTGCACCTAAGCTCGAAGGGCAGAT
GGGAGAGGATGGAAACTCTATTAAAGTGAACCTGATCAAGCAGGATGACGGCGGCTCCCC
CATCAGACACTATCTGGTCAGGTACCGAGCGCTCTCCTCCGAGTGGAAACCAGAGATCAG
GCTCCCGTCTGGCAGTGACCACGTCATGCTGAAGTCCCTGGACTGGAATGCTGAGTATGA
GGTCTACGTGGTGGCTGAGAACCAGCAAGGAAAATCCAAGGCGGCTCATTTTGTGTTCAG
GACCTCGGCCCAGCCCACAGCCATCCCAGCCAACGGCAGCCCCACCTCAGGCCTGAGCAC
CGGGGCCATCGTGGGCATCCTCATCGTCATCTTCGTCCTGCTCCTGGTGGTTGTGGACAT
CACCTGCTACTTCCTGAACAAGTGTGGCCTGTTCATGTGCATTGCGGTCAACCTGTGTGG
AAAAGCCGGGCCCGGGGCCAAGGGCAAGGACATGGAGGAGGGCAAGGCCGCCTTCTCGAA
AGATGAGTCCAAGGAGCCCATCGTGGAGGTTCGAACGGAGGAGGAGAGGACCCCAAACCA
TGATGGAGGGAAACACACAGAGCCCAACGAGACCACGCCACTGACGGAGCCCGAGAAGGG
CCCCGTAGAAGCAAAGCCAGAGTGCCAGGAGACAGAAACGAAGCCAGCGCCAGCCGAAGT
CAAGACGGTCCCCAATGACGCCACACAGACAAAGGAGAACGAGAACAAAGCATGATGGGT
GAAGAGAACCGAGCAAAGATCAAAATAAAAAGTGACACAGCAGCTTCACCAGAGCATTTC
CAACACCACAGACACACACACGCACGCACACACACAAACACACATGCACACACACACATC
TCATTTCTCTAGTGTCTTTTGCCTTTAAAAAAAACTAAACAGATAAAACATGGGAATCTC
CTTTTTGTAGGTTTATAGAAAGGGTCCCTTTGTTGCACACTCACTTGTAAGAAAATGAGA
CAAAAAGGTTAAACCCACAGCCAAACTAGGACACTCCGTTCCCTGAAACCGTTAAAAAAT
CAAACAAAAGGACCCCAAATTAAGAATCTAGGAAGCTCAGAAACGAAATCTAGGTTCAGG
AAGACCACACTTGGTGTTACCCGATTGGCACAGACCAGTTTCAGAGAAATACTTTCAGGC
ACTAAGACTAATCGAATGAACAAAGTCCACAGTTTATTTTTATACTTTCAGTCAAGTTTG
AACTCTGTAAAACCTCATAAATAAGTTATAATTTCTGTTCACTTTGTATTTGTTCAGTAT
GCAAAGTGTGTCACCCTTTCTAGCTGAATTCAATTCCCACGTAGACTCTTATTTTATAGG
ACGAATGCCAAATTGCAGCTTCTGGGGGTAGATCTCAATTTGCAGTATTCAGACTTCTTT
TTCTTTCTTTTACATTCTTTTTTCTTTCTTTCTTTCTGCCAACTTTGTTTTCCAGTGTTT
ACAAGGTGACAAATGTTTGACTTTGGTTGTGTTTAAATGTCCGTGTAAAATAGCTGCCTT
TTATTTTTTAAGGTAACAAATACCACCTAGAGGTAGGTAGGATCATCCCACGCTTGCTTT
AGCACAGGACAACTTTACAAAACATGATTGTTTACAGCTGCTCTTCCCCTCTTTTCTGAT
CTGCAGTTTTTGCCTGGGTCCCACTCAGGTGAAAATCCATCTCATTCTGGAATGGTTTTG
CTTTTGAATTTTTGGTTATTTTTGTGTTTCTTTGGGGGTTAGACCACTTTCTGATTAGCC
GCCACCTGCCTGCATCTGTGAAAAGGGATCTGCTCCCAGGCGTTCTCACCCTTCTTTTGA
AGGACTCCTTAGGCTTTGTTGAATGAAGCAGAGAAGATTGTATAGTTGGGGCTGGTCTTG
GTGAACACACATTATTACCCCACACATCCCCTTTGTGTAGAAAGCCAAATAAAATCTATA
CATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MLQTKDLIWTLFFLGTAVSLQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGE KLTPNQQRISVVWNDDSSSTLTIYNANIDDAGIYKCVVTGEDGSESEATVNVKIFQKLMF KNAPTPQEFREGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIK KTDEGTYRCEGRILARGEINFKDIQVIVNVPPTIQARQNIVNATANLGQSVTLVCDAEGF PEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKKVDKNDEAEYICIAENKAGEQDATI HLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPE KQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQG PVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDS ENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDQVEPYSSTAQVQFDEPEATGGVPI LKYKAEWRAVGEEVWHSKWYDAKEASMEGIVTIVGLKPETTYAVRLAALNGKGLGEISAA SEFKTQPVQGEPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVRYRALSSEWKPEI RLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTSGLS TGAIVGILIVIFVLLLVVVDITCYFLNKCGLFMCIAVNLCGKAGPGAKGKDMEEGKAAFS KDESKEPIVEVRTEEERTPNHDGGKHTEPNETTPLTEPEKGPVEAKPECQETETKPAPAE VKTVPNDATQTKENENKA*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
