Gene:
Gene Symbol:
TFCP2
Gene Name:
transcription factor CP2
Original Clone ID:
FLH271290.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618218
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1509nts
Open reading frame: 469 to 1977
GGCACGAGGGAGAGAGCGAGACCTGGGAGGGAGGGGTCCTCCAGCAGAAAGGGGCGGGGG
AAAAGGTGCAAAAGCAGCGTGGGAGCGCCGGGCTGGCTTCCTGCGGCTGCTGCTGGTCTG
ACTGGGAAGCAGCAAGCCACCACTACGAACTCTCAAGAGGAGTGGGAGTGCGGGAGTCCA
GAGCTGCCTCTGGGAAGTCTGCAGTAGTTGAGCAAAGGGGTCCTCACGTTCCTGAGAGCT
GGGCAGGGGGGATTTTGGAACCTGGGGCAGCCAAGAACGAGCAGCCAAGGGTACGGGAGA
TTAGTTGTGCACAGAGCAGTGCTAGTCGGGCTTGGGGGTGGCTGGTGGGCACTGCGTGGG
AAACCTTGGTTTGTAGTTTTCTTGGTTTGCGTTACTCCTGTTGGGTAGAATTACCCTCCG
CGCCTTTGTACAAGACACGGTGTCTCCTGGGGCAAGGAAGGAGCCAGGATGGCCTGGGCT
CTGAAGCTGCCTCTGGCCGACGAAGTGATTGAATCCGGGTTGGTGCAGGACTTTGATGCT
AGCCTGTCCGGGATCGGCCAGGAACTGGGTGCTGGTGCCTATAGCATGAGTGATGTCCTT
GCATTGCCCATTTTTAAGCAAGAAGAGTCGAGTTTGCCTCCTGATAATGAGAATAAAATC
CTGCCTTTTCAATATGTGCTTTGTGCTGCTACCTCTCCAGCAGTGAAACTCCATGATGAA
ACCCTAACGTATCTCAATCAAGGACAGTCTTATGAAATTCGAATGCTAGACAATAGGAAA
CTTGGAGAACTTCCAGAAATTAATGGCAAATTGGTGAAGAGTATATTCCGTGTGGTGTTC
CATGACAGAAGGCTTCAGTACACTGAGCATCAGCAGCTAGAGGGCTGGAGGTGGAACCGA
CCTGGAGACAGAATTCTTGACATAGATATCCCGATGTCTGTGGGTATAATCGATCCTAGG
GCTAATCCAACTCAACTAAATACAGTGGAGTTCCTGTGGGACCCTGCAAAGAGGACATCT
GTGTTTATTCAGGTGCACTGTATTAGCACAGAGTTCACTATGAGGAAACATGGTGGAGAA
AAGGGGGTGCCATTCCGAGTACAAATAGATACCTTCAAGGAGAATGAAAACGGGGAATAT
ACTGAGCACTTACACTCGGCCAGCTGCCAGATCAAAGTTTTCAAGCCCAAAGGTGCAGAC
AGAAAGCAAAAAACGGATAGGGAAAAAATGGAGAAACGAACACCTCATGAAAAGGAGAAA
TATCAGCCTTCCTATGAGACAACCATACTCACAGAGTGTTCTCCATGGCCCGAGATCACG
TATGTCAATAACTCCCCATCACCTGGCTTCAACAGTTCCCATAGCAGTTTTTCTCTTGGG
GAAGGAAATGGTTCACCAAACCACCAGCCAGAGCCACCCCCTCCAGTCACAGATAACCTC
TTACCAACAACCACACCTCAGGAAGCTCAGCAGTGGTTGCATCGAAATCGTTTTTCTACA
TTCACAAGGCTTTTCACAAACTTCTCAGGGGCAGATTTATTGAAATTAACTAGAGATGAT
GTGATCCAAATCTGTGGCCCTGCAGATGGAATCAGACTTTTTAATGCATTAAAAGGCCGG
ATGGTGCGTCCAAGGTTAACCATTTATGTTTGTCAGGAATCACTGCAGTTGAGGGAGCAG
CAACAACAGCAGCAGCAACAGCAGCAGAAGCATGAGGATGGAGACTCAAATGGTACTTTC
TTCGTTTACCATGCTATCTATCTAGAAGAACTAACAGCTGTTGAATTGACAGAAAAAATT
GCTCAGCTTTTCAGCATTTCCCCTTGCCAGATCAGCCAGATTTACAAGCAGGGGCCAACA
GGAATTCATGTGCTCATCAGTGATGAGATGATACAGAACTTTCAGGAAGAAGCATGTTTT
ATTCTGGACACAATGAAAGCAGAAACCAATGATAGCTATCATATCATACTGAAGTAGGAG
TGCGGCGTTTCGTGCCCAGTGGCTGCTCCTTCCTTCACCTCTGAAAACGGCCCTCTTGAA
GGGGGATATGAATGGAGATTTGAAGGTCTGCAAGAACCTGACTCGTCTGACTGTGTGTGG
AGGAGTCCAGGCCATGGAGGCAGAATCCTGGCCCTCTGTGTTGGCCCAAGCTCTTGTGGT
ACACACAGATTACTGCCCAATATGCAGTTCTGCAGCTGTTTTAGTTAAATTTCTGGACCT
TGTTGTTGTTAAATATCAGTAGAAACTCTACATAATTTAGAGTGTATGTAGGGCATAATG
ATGATGGGAATTGTGTGATGTTTAACAGGAAGATCTTAAATTTTGTGATATGGAGCCCTG
TAATTTTTTTCTTATATAAAAATGGGTATCTATATTCATAAAAAAAAAAAAAAAAAAAAA
AA
Protein sequence predicted based on nucleotide sequence
MAWALKLPLADEVIESGLVQDFDASLSGIGQELGAGAYSMSDVLALPIFKQEESSLPPDN ENKILPFQYVLCAATSPAVKLHDETLTYLNQGQSYEIRMLDNRKLGELPEINGKLVKSIF RVVFHDRRLQYTEHQQLEGWRWNRPGDRILDIDIPMSVGIIDPRANPTQLNTVEFLWDPA KRTSVFIQVHCISTEFTMRKHGGEKGVPFRVQIDTFKENENGEYTEHLHSASCQIKVFKP KGADRKQKTDREKMEKRTPHEKEKYQPSYETTILTECSPWPEITYVNNSPSPGFNSSHSS FSLGEGNGSPNHQPEPPPPVTDNLLPTTTPQEAQQWLHRNRFSTFTRLFTNFSGADLLKL TRDDVIQICGPADGIRLFNALKGRMVRPRLTIYVCQESLQLREQQQQQQQQQQKHEDGDS NGTFFVYHAIYLEELTAVELTEKIAQLFSISPCQISQIYKQGPTGIHVLISDEMIQNFQE EACFILDTMKAETNDSYHIILK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
