Gene:
Gene Symbol:
SLC37A3
Gene Name:
solute carrier family 37, member 3
Original Clone ID:
FLH271334.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618225
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1437nts
Open reading frame: 158 to 1594
CTGGCGGCGGGGGAGGCGGGCAGCGGAGCCAAGCTGACCCGGCGAGCGGAGCCGGGGCTG
GAGAGCGGCGACCACTGCGGATCTCGGAAGGAAGAAATGATGTAAATCACTCATCCAAAC
CTTAAGGTCAAAGGTGAGAAGGAAGGTCAGGAAGAACATGGCCTGGCCAAATGTTTTTCA
AAGAGGGTCTCTGCTGTCCCAGTTCAGCCATCATCATGTTGTAGTGTTCCTGCTCACTTT
CTTCAGTTATTCGTTGCTCCATGCTTCACGAAAAACATTTAGCAATGTCAAAGTCAGTAT
CTCTGAGCAGTGGACCCCAAGTGCTTTTAACACGTCAGTTGAGCTGCCTGTGGAGATCTG
GAGCAGCAACCATTTGTTCCCCAGTGCAGAGAAAGCGACTCTTTTCCTCGGCACACTGGA
TACCATTTTCCTCTTCTCCTATGCTGTGGGCCTATTCATCAGTGGCATCGTTGGGGATCG
GTTGAATTTGCGATGGGTTCTGTCTTTTGGCATGTGCTCTTCTGCATTAGTGGTGTTTGT
CTTTGGTGCGCTCACAGAATGGCTGCGTTTCTACAACAAATGGCTGTACTGCTGCCTGTG
GATTGTGAACGGCCTGCTGCAGTCCACTGGTTGGCCCTGTGTGGTTGCTGTTATGGGCAA
CTGGTTTGGGAAAGCCGGACGAGGAGTTGTTTTTGGTCTCTGGAGTGCCTGTGCTTCGGT
GGGCAACATTTTGGGAGCGTGCCTAGCTTCTTCTGTTCTTCAGTATGGTTATGAGTATGC
CTTTCTGGTGACGGCGTCTGTGCAGTTTGCTGGTGGGATCGTTATCTTCTTTGGACTCCT
GGTGTCACCAGAAGAAATTGGTCTCTCGGGTATTGAGGCAGAAGAAAACTTTGAAGAAGA
CTCACACAGGCCATTAATTAATGGTGGTGAAAATGAAGACGAATATGAGCCGAATTATTC
AATCCAAGATGATAGTTCTGTTGCCCAAGTCAAGGCGATAAGCTTCTACCAGGCATGTTG
CCTTCCTGGAGTCATACCGTACTCACTGGCCTACGCCTGCTTGAAGTTAGTGAATTACTC
CTTCTTCTTCTGGCTCCCCTTTTATCTGAGTAACAACTTCGGCTGGAAGGAGGCGGAAGC
CGACAAGCTGTCCATTTGGTACGACGTTGGAGGGATCATAGGTGGAACTTTGCAAGGCTT
CATCTCTGATGTACTACAGAAGAGAGCGCCGGTTCTTGCCCTGAGTCTGCTTCTGGCAGT
TGGGTCCCTCATCGGGTATAGTCGATTTTTTATTGGTGGACCTTCTAATATGATTAGTTC
TGCTATTTCTGCGGACTTGGGTCGCCAGGAGCTCATCCAAAGGAGCAGTGAAGCTTTGGC
CACTGTCACAGGAATTGTGGATGGTTCGGGGAGCATTGGAGCTGCAGTGGGCCAGTATTT
AGTGTCTCTGATCCGGGACAAGCTAGGATGGATGTGGGTTTTCTACTTTTTCATTCTCAT
GACAAGTTGTACAATTGTGTTTATCTCGCCATTAATAGTGAGGGAAATATTCTCTCTCGT
GCTAAGGAGACAGGCTCACATATTGAGGGAGTGACCGGTGCCCGCGAGACAGAAAGAACA
ATGTCAGCCACATCAGGACCGCTGGGGCTTCGAGTCTGCCCTAATTTGGGTTTGTCTAGG
AGCTCCAGCCTGATCTTGGATTGTCAGGCGTCTCTCAACACTGCCAGCCACCTGAGATGC
TGACCAGCAGTGAAGGCTGCACTAGTTTTCTACACTACAGGAATTACTGTTGATGATTTT
TGCTGTTGTTTCATGAATGTGCCGAGTGACTTCTGACTCTGCCAGCATCTTTGACCTCTG
ACTCTGCCCGCGTCTTTGTTTGGCCTATTGAGGTTTAGCGTTTTAACCTTAACTGTTGTT
TTGGACTTGAACTTTCTCTAGCCTTGAAAATGGAGTGAACAGGAGGCAAAGCCACGCGTG
GTTTGTGTTGGTGGTATGTTCCACAAATGGAAGAGATCATTGCATTTTGCTTTGGTAATG
AGTTTACAGAGAGTGGGTCATCTTTGAAGCATCAAATCCTGATCAAATGCTCAAGCTGGA
CCGAGCTGCAAGCCTGCCTTGGACAAAGAATCCCTCTGTTCACTGTGGTGGGAAAGATCA
CACTACATGCCTGTTGATTGGCTCAGTCACTCTGTGTCTGATCTAAACGTCATTCAGCCC
TAGAAGCATGAATTGCTTCAAATTATTGTCAACTTGTTCTCTTCCATTTTCATTCAAATT
AACTTTGACTCCTGGAAGTATTGAGTCTTCCTTTCAAGGACCATAAGGTACATCAGTTAT
CTTGAACATTCTGACGTGTAAAGGACCATAAGGTACATCATTTATCTTGAACATTCCGAC
GTGTAACTCACAGCCGAAGCACTCCATCCCAGATTTGTTGTCTGGGAACTTAGGATCCTC
TAGGAAGCTAATCTGCTTAGTCTATTTTTAGAGGATTGATCTCTGGCACAAGCAGCTTCC
TGGAGTTACCTCAGCAGAAGACTAGAATTAGAGAAAAGGGAAAGACCTTTTCTTCTAGTA
AGTGTCAAGTACAGATGCTCTTTGACTTATGATGGGGTTATGTCCCAGTAAACCCATTGG
AAGTCAAATGCATTTAATACCCCTGACTGGACACCACAGCTTAGCCTCGTCCACCCTAAA
TGTACTCAGAACACTTAGAATTGCCCACAGTTGGGCAGAATCATCTAACACAAACTCTAT
TTTATAATCGAGTGTTGAATATATCATGTAATTTATTGAATATTGTACATTATGTTGAAA
TTGCAACCATTTCACACCATTGTAAAGTCCAAAAATCAGAAGCTGAGGACTGTCTGTACA
TGACATTGAATCACATGAATGGATTACTAGGGCCTGAGGGGTGTCCGTTCTTTAGAAAAA
TGTAGAGTATATATATCTTCAGGGCTGTGAATTCCATACTAGTTATCAATATTTTAAGAT
CATTCTGTATATACATATTTTAAATTATGCAAGCAAACTAGGAGGAGAATGTGCAATTTT
GAGGGATACCTAATTTGCATTCGGTTAGGGGATATTTTTCAACCTCTTGCTTTATACTCT
GATATGGGGTGTGTTTATGAACTTGTTAATGTTGTTTAATCATGCAGATTTCCAATCAGT
GTATTGCTCATTTACTGACAAAAAGGGAAAATACGAAACACAGAAAGGGAAAATAAACTG
ACTTTTATAATGAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MAWPNVFQRGSLLSQFSHHHVVVFLLTFFSYSLLHASRKTFSNVKVSISEQWTPSAFNTS VELPVEIWSSNHLFPSAEKATLFLGTLDTIFLFSYAVGLFISGIVGDRLNLRWVLSFGMC SSALVVFVFGALTEWLRFYNKWLYCCLWIVNGLLQSTGWPCVVAVMGNWFGKAGRGVVFG LWSACASVGNILGACLASSVLQYGYEYAFLVTASVQFAGGIVIFFGLLVSPEEIGLSGIE AEENFEEDSHRPLINGGENEDEYEPNYSIQDDSSVAQVKAISFYQACCLPGVIPYSLAYA CLKLVNYSFFFWLPFYLSNNFGWKEAEADKLSIWYDVGGIIGGTLQGFISDVLQKRAPVL ALSLLLAVGSLIGYSRFFIGGPSNMISSAISADLGRQELIQRSSEALATVTGIVDGSGSI GAAVGQYLVSLIRDKLGWMWVFYFFILMTSCTIVFISPLIVREIFSLVLRRQAHILRE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
