Gene:
Gene Symbol:
STK4
Gene Name:
serine/threonine kinase 4
Original Clone ID:
FLH231606.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618241
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1464nts
Open reading frame: 91 to 1554
GCGGAAGTGTGGGAGGGTCTGCGGGGCGGGCTCAGGAGGTCCGCGGGAGGATGGAGCAGT
GAGCGGGTCTGGGCGGCTGCTGGCAGCGCCATGGAGACGGTACAGCTGAGGAACCCGCCG
CGCCGGCAGCTGAAAAAGTTGGATGAAGATAGTTTAACCAAACAACCAGAAGAAGTATTT
GATGTCTTAGAGAAACTTGGAGAAGGGTCCTATGGCAGCGTATACAAAGCTATTCATAAA
GAGACCGGCCAGATTGTTGCTATTAAGCAAGTTCCTGTGGAATCAGACCTCCAGGAGATA
ATCAAAGAAATCTCTATAATGCAGCAATGTGACAGCCCTCATGTAGTCAAATATTATGGC
AGTTATTTTAAGAACACAGACTTATGGATCGTTATGGAGTACTGTGGGGCTGGTTCTGTA
TCTGATATCATTCGATTACGAAATAAAACGTTAACAGAAGATGAAATAGCTACAATATTA
CAATCAACTCTTAAGGGACTTGAATACCTTCATTTTATGAGAAAAATACACCGAGATATC
AAGGCAGGAAATATTTTGCTAAATACAGAAGGACATGCAAAACTTGCAGATTTTGGGGTA
GCAGGTCAACTTACAGATACCATGGCCAAGCGGAATACAGTGATAGGAACACCATTTTGG
ATGGCTCCAGAAGTGATTCAGGAAATTGGATACAACTGTGTAGCAGACATCTGGTCCCTG
GGAATAACTGCCATAGAAATGGCTGAAGGAAAGCCCCCTTATGCTGATATCCATCCAATG
AGGGCAATCTTCATGATTCCTACAAATCCTCCTCCCACATTCCGAAAACCAGAGCTATGG
TCAGATAACTTTACAGATTTTGTGAAACAGTGTCTTGTAAAGAGCCCTGAGCAGAGGGCC
ACAGCCACTCAGCTCCTGCAGCACCCATTTGTCAGGAGTGCCAAAGGAGTGTCAATACTG
CGAGACTTAATTAATGAAGCCATGGATGTGAAACTGAAACGCCAGGAATCCCAGCAGCGG
GAAGTGGACCAGGACGATGAAGAAAACTCAGAAGAGGATGAAATGGATTCTGGCACGATG
GTTCGAGCAGTGGGTGATGAGATGGGCACTGTCCGAGTAGCCAGCACCATGACTGATGGA
GCCAATACTATGATTGAGCACGATGACACGTTGCCATCACAACTGGGCACCATGGTGATC
AATGCAGAGGATGAGGAAGAGGAAGGAACTATGAAAAGAAGGGATGAGACCATGCAGCCT
GCGAAACCATCCTTTCTTGAATATTTTGAACAAAAAGAAAAGGAAAACCAGATCAACAGC
TTTGGCAAGAGTGTACCTGGTCCACTGAAAAATTCTTCAGATTGGAAAATACCACAGGAT
GGAGACTACGAGTTTCTTAAGAGTTGGACAGTGGAGGACCTTCAGAAGAGGCTCTTGGCC
CTGGACCCCATGATGGAGCAGGAGATTGAAGAGATCCGGCAGAAGTACCAGTCCAAGCGG
CAGCCCATCCTGGATGCCATAGAGGCTAAGAAGAGACGGCAACAAAACTTCTGAGCAAGG
CCAGGCTGTGAGGGCCCCAGCTCCACCCAGGCTTTGGGTGAATTCTGGATGGCTTGCCTC
ATGTTTGTTAGCCAGCACTTCTGCTCTGTCGTCTCTCCACAGCACCTTTGTGAACTCAGG
AATGTGCGCCAGTGGGAAGGGCTCTCTTGACAGTCAGCGTGCCATCTTGATGTGTGTATG
TACATTGGTCAGGTATATTATCTCAAAGGATTTATATTGGCGCTTTTAACTCAGAGTTTT
AAACCCCAGGAACAGAGACTCCTAGTTGAGTGATAGCTGGGAAAGTTTTACATTGTCTGT
TTTTCTTCTCCCAATAGCTTTCAATTGTTCTTTCTGGAAGACTTTTAAAAAAATATAAAT
ATGCATATATATATATAAATTATAAATAGATTCCCCACGCAGTGTGGTGGCATCTCTGTA
CAGGTACAGTTTTAAACGGTTTGCCTCTTTTCTGTAAGATTATGGTACTGTGGAACATGA
GGGCAGAGGACACCGGGAGGCTGTTAGGGGGTCACTGAATCCCAGGAGCCAACCTCCCCC
TTTGCAGGGCTGCATTTAAAAATTAGGTTTGGGACAGTTCTTGTACCGTGGTTTCAGCCT
TGTGTGGTCATCACTGGCTTCTGGAGCTATTGGTGATGTCCAAGGGAAAGCTTTGAGAGT
TTATGTTTACTCTTTGAGTCCCAGGAGAAGCCTGGCACCCTCTTTGCAAATTGGCCTTTG
CTCTTTCAATGCCTTTCATCCATCTCCACTCTCTCAACTGCCTAAAGTCACAGCACAGAT
ACTGCCCAGTGCCTTAAGAGGAGACATGATCTCTACCAGGGACTCTCAGCAAACACGGGA
CTGTGTTCAGTCCACAAAGGAAAAGCGTTTTTGAAGCTCTCATTGTTCATGTAAAAATCA
TACACGTGGCATGTTGCTCCACATTCCTTACACACAGGGGTAGAGGGGATTGCTTTTGTG
ACCCACGTTCAAATATGTGACTGTTTTCTTTTCTCTTTTACTGCTAAGCAGCCTGGAAAG
GATAAATGAATATTAGACTAAGATTTGTTTTCCAGGAGGCTCAATCTGAACACACAGAAT
GTCAGAGCTGGAAGGGACTATAGAGATCATCTGATCTGATCCTCTTGTACGGATGATCGC
AAAACTGAGGTGTAGAGAGGGGAATGGCCAAAATCACAAAGCAAGTTAGCGTTAAGAGCT
GAGACTAGAATTCAGGGTCCTCACTCCCAGGCCACCGAACCATGCAGCCCCTTCTTTGGG
GGAAGAGACCTGTGTCAGTCTTGGTTAATTGTTCCAGGGAACCTTGCTAACAGAAACTTG
CTCTTGCCTTGGCTCTTCAGTAGATGACCTGGCTGTAAAGAGATTCCCTGGACGAGCCAG
ATCATTCAGTTTCAGCGAGTCCTTGAGCTCCACAACATCTACCAGATATAGCAGACAAGC
ACCCATGGAGGCAGGTTTCGGGCCTGAAGCAGATCAGAGGGCTTTGCAAAAGACAGCATA
GAGCCATCTTCCTGCAACTTTACCTCTTTCCCTCAGATGGGGAGCCATGACTGGGTTGCA
CCTCAGGATACTGTAATTTGACTCCATAATTGCTTTTGCTCCTGAAACCTGGGAATCAAT
GGAAAGGCAGGGAATGTGCCTCTTCTGTGGCCAGATTCTGTTATTTGCAATTAAAGCAAG
TTTTTAAAAAATGCAAGAGGCAGTTGTTAGTCTTCAGGGCTTGGCAACTGAAATAGCTAT
GTGGCGGATACGGAAAACAGAGGACAATTTGAGGATCTTGCTGGAATAATAAATGACAGC
TACCATTTGTTGAGCACCTATTATATATCAGGCACTGAGCTGGGTAGGCTCTAAACTTCA
CAATAACCCTGTGACTTAACTACTTTATCTCCATTTTGTAGTTGAAGAAATAAGTTCAGA
GAGAAAGATTCCTTCCCAAGGTCATGCAGCTAGTAAATGATAGAATCAGGATTCATAGCA
TCACTATAGGGGGTCAATATTTACACAAAAAAGGAAAGTCACAAGCCTGTTTAAAATGAA
GTGACCACCTTTTCTTGCATAGACTAAATAACTCGAACTGGCATTTTTAGGTTGGAAAGA
CAGCTGAATTAGTAGTTAAGTCTGATAGCCAAGTAAGTTTTAAAAACCAAAGCATCCAGG
ATGCACACCCCTGCACCATTTGCTGTGCGAATTAATAGTTCTGTCTCTCTCTCTCTTTCT
TTTTTCTTTTTATTCTTTGAGATGGATTTTCGCTCTTGTCGCCCAGGCTGGAGTACAATG
GCACGATCTTGGCTCACTGCAACCTCCGCCTCCCGGGTTCAAGCGATTCTTCTGCTGGGA
TTACAGCATATGCCACCATGCCCAGATTATTTTTTTGTATTTGTAGTAGAGACGGGGTTT
CACCATGTCAGTCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCACCCGCCTCAGCC
TCCCACACTGCTGGGATTACAGGCATGAGCCACCGCTCCTGGCCTCTCTTTCTTTTTTAA
ACAAAGAACTTTGCACTTGGCCAGAGAGGAGGAGAAAGCCCATTTTCTCCCTTCCTAAGC
TAGATCCAAATAAAAGAAAGTTCAGTTTTCCCCCATAACTATTCTTGGGTCATGAACTTT
GATCTGGAGTTTGTTTTGTTTCAGGAATGTGTGCACCCAGCTTGCTGATCCAACAAAGTC
TATTGCTTACCAGTCTAGCTTGATGAAGCCTTTTGGCCAGAAGTCAATTTGTTTTGGATC
AGAGAAATTTCCTGACAAGGTATATTTGTTTTCTAGTGACAGAAAGGCAAAGGAACAAGT
CCTAGTTGTTGTTGTTGTTGTTGAATACTAAATTTAAGATATGTCAGCTTGCTTTCAATG
AGCCTTGGGCTTCTGTTATTGCTTGAGCATTTGGAACTCGAGCTTCCAGAGAAATTTGAG
GTCCTCGCTTGTTCTCTGCCTTCAAGAAACAATGACCTGATTCTGTCTTTAAAAAAAAAA
ATCTCAGAATTCTTTTTTTGTTTGTGTTTTTTTTTTTTTTTGAGACAGAGTCTCACTCTG
TTGCCCAGGCTGGAGTGCAGTGGCGCCATCTCGGCTCACTGCAACCTCCGCCTCCCAGGT
TCAAGCAATTCTCCTGCCTCAGCCTCCCAGGTAGCTGCCACTACAGGTGCTGCACCACCA
CGCCCGGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATATTAGCCAGGTGGG
TCTTGAACTCCTGACCTTGTGATCCACCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAG
GCGTGAGCCACCTTGCCTGGCCAAAAATCTCAGAATTCTTTAAGACTGTTTTAATTGCTC
CATCAGTAATTTTGAAGCACTTTCCTTTTTTTTTTTTTTTCCCCTTTTTGTCCCTTTCCC
CAAGCCACCAATTGGATGGATGAATGTTTGACGGGGAAGAGGAAGGGTAGGAGGATGCAT
GGATGAGTGGATGAGTGGATCGATGGATGTATTGATAAATAGATAGAACCAGTCATCTGA
AGCAACTTAAGAATTGTAGCCTTGACTCCTTGAGACTGTAGATTTCGATCCAGGAAACAT
TTATTTAGCACCTGCCAGATGCCAGAAATTTATACCATTTAAAACTCAGTAAGTCTTTTA
AATATCAGGAAGGAGAGAAGCGACATCATGATACATCCTATGGGTATTAAAAAGCCAATA
GAATATTATGAATAATTTTATGCTAATAAATTTAACAACTTCAACATCATAAACAAATTC
CTTGAAAAATAAAAAGTACCAAAATTCATTCAAGAAGAAATAGATACCAGCCTGAGCAAC
ATGGCAAAATCCCATCTCTACAAAACATCAAAAAAAAAAAAAATTAGTCGGGCATGGTGG
TGCACACCTGTAATCCCAGCTTGTCAGGAGGCTGAAGTGGGAGGATCACCTGAGCCCAGG
GAGGTCAAGGATGCAGTGAGCCATGGTCTCACCACTGCACTCTAGCCTGGGTGACAGAAT
GAGACCCCGTCTCAAAAAAAAAGAAGAAGTAGATAATCTGAATAGCCCTATATCTATAGA
AACTTAATAGTGCTGGGAGATATAGGTATTATTATCCTCATTTTACAGATGTGAAAATTG
AGGCTCAGAGAAGTAAAGTCTATTGCTCAAGGTCATGTGGCTAGAATATGGCAGAGCCAT
GATTCAGATCCAGGTCTTCTGATTCTTATTCCAGTGTCCTTTCTAGCATACCATGTTGCC
TCTAAAGATTGCAGCTCCTTATTTACTAGAAAATTGTTCCTGCCCAATCTACATCTCCAC
CTCACCCCATCTTTTCTTAAGCACTATGTTTGTGTTTTTATCAGTATTATATTCATTGTC
TTTGGAATACATGTTCTTGTTTGTGTTTGGAAAAAAAATCTCTTTTACCAGCTTGCACTC
GGACCAACTTGGAAAAAAAAAAGCTTAAATGTTTTTGCTATGTACAGTTTAAAAATGTGA
AGTTTGTAGCTTTAACTTTTTGTAAGAAAATCTAATAACACTGGCTTAAGTGCTGACTTG
AAATGCTATTTTGTAAGGTTTGGATGTAAGTAATCAATTGAGGTCAGCAGTTTGTATGAG
ACATAGCTTCCTCCATTGCCCCCACTCCTTTTTTCTTTTTTAAGTTTGAGATGCTTCCTG
TGTTTTTATGTTAGAATTGTTGTTCTCCTTCTTTTCTTCTTCCTATACCTCATCACGTTT
GTTTTAAATAAACTGTCCTTTGGACCACAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
METVQLRNPPRRQLKKLDEDSLTKQPEEVFDVLEKLGEGSYGSVYKAIHKETGQIVAIKQ VPVESDLQEIIKEISIMQQCDSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKT LTEDEIATILQSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNP PPTFRKPELWSDNFTDFVKQCLVKSPEQRATATQLLQHPFVRSAKGVSILRDLINEAMDV KLKRQESQQREVDQDDEENSEEDEMDSGTMVRAVGDEMGTVRVASTMTDGANTMIEHDDT LPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFEQKEKENQINSFGKSVPGPLK NSSDWKIPQDGDYEFLKSWTVEDLQKRLLALDPMMEQEIEEIRQKYQSKRQPILDAIEAK KRRQQNF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
