Gene:
Gene Symbol:
SLK
Gene Name:
STE20-like kinase
Original Clone ID:
HsCD00299380
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618245
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
CTTAAGTGCAAGGAACTCTGTGTTGGGAGGAAAAATGTCCTTCTTCAATTTCCGTAAGAT
CTTCAAGTTGGGGAGCGAGAAGAAGAAGAAGCAGTACGAACACGTGAAGAGGGACCTGAA
CCCCGAAGACTTTTGGGAGATTATAGGAGAACTGGGCGACGGAGCCTTTGGGAAAGTGTA
CAAGGCCCAGAATAAAGAGACCAGTGTTTTAGCTGCTGCAAAAGTGATTGACACTAAATC
TGAAGAAGAACTTGAAGATTACATGGTAGAGATTGACATATTAGCATCTTGTGATCACCC
AAATATAGTCAAGCTTCTAGATGCCTTCTATTATGAGAACAATCTTTGGATCCTCATTGA
ATTTTGTGCAGGTGGAGCAGTAGATGCTGTGATGCTTGAACTTGAGAGACCATTAACTGA
GTCCCAAATACAAGTAGTTTGCAAGCAGACTTTAGATGCATTGAACTACTTACATGATAA
TAAGATCATCCACAGAGATCTGAAGGCTGGCAACATTCTCTTTACCTTAGATGGAGATAT
CAAATTGGCGGATTTTGGAGTATCAGCTAAAAACACGAGGACAATTCAAAGAAGAGATTC
CTTTATTGGTACACCATATTGGATGGCTCCTGAAGTAGTCATGTGTGAAACATCTAAGGA
CAGACCCTATGACTACAAAGCTGATGTTTGGTCCCTGGGTATCACTTTAATAGAAATGGC
TGAGATAGAACCACCTCATCATGAATTAAATCCAATGCGAGTGCTGCTAAAAATAGCAAA
ATCTGAGCCACCTACATTAGCACAGCCATCCAGATGGTCTTCAAATTTTAAGGACTTTCT
AAAGAAATGCTTAGAAAAGAATGTGGATGCCAGGTGGACTACATCTCAGCTGCTGCAGCA
TCCCTTTGTTACTGTTGATTCCAACAAACCCATCCGAGAATTGATTGCAGAGGCGAAGGC
TGAAGTAACAGAAGAAGTTGAAGATGGCAAAGAGGAAGATGAAGAGGAGGAAACAGAAAA
TTCTCTGCCAATACCTGCAAGTAAGCGTGCATCTTCTGACCTTAGTATCGCCAGCTCTGA
AGAAGATAAACTTTCACAAAATGCTTGTATTTTGGAGTCTGTCTCAGAAAAAACAGAACG
TAGTAACTCTGAAGATAAACTCAACAGCAAAATTCTTAATGAAAAACCCACCACTGATGA
ACCTGAAAAGGCTGTGGAGGATATTAATGAACATATTACCGATGCTCAGTTAGAAGCAAT
GACTGAACTCCATGACAGAACAGCAGTAATCAAGGAGAATGAAAGAGAGAAGAGGCCCAA
GCTTGAAAATCTGCCTGACACAGAAGACCAAGAAACTGTGGACATTAATTCAGTCAGTGA
AGGAAAAGAGAATAATATAATGATAACCTTAGAAACAAATATTGAACATAATCTAAAATC
TGAGGAAGAAAAGGATCAGGAAAAGCAACAGATGTTTGAAAATAAGCTTATAAAATCTGA
AGAAATTAAAGATACTATTTTGCAAACAGTAGATTTAGTTTCTCAAGAGACTGGAGAAAA
AGAGGCAAATATTCAGGCAGTTGATAGTGAAGTTGGGCTTACAAAGGAAGACACCCAAGA
GAAATTGGGGGAAGACGACAAAACTCAAAAAGATGTGATCAGCAATACAAGTGATGTGAT
AGGAACATGTGAGGCAGCAGATGTGGCTCAGAAAGTGGATGAAGACAGTGCTGAGGATAC
GCAGAGTAATGATGGGAAAGAAGTGGTCGAAGTAGGCCAGAAATTAATTAATAAGCCCAT
GGTGGGTCCTGAGGCTGGTGGTACTAAGGAAGTTCCTATTAAAGAAATAGTTGAAATGAA
TGAAATAGAAGAAGGTAAAAATAAGGAACAAGCAATAAACAGTTCAGAGAACATAATGGA
CATCAATGAGGAACCAGGAACAACTGAAGGTGAAGAAATCACTGAGTCAAGTAGCACTGA
AGAAATGGAGGTCAGAAGTGTGGTGGCTGATACTGACCAAAAGGCTTTAGGAAGTGAAGT
TCAGGATGCTTCTAAAGTCACTACTCAGATAGATAAAGAGAAAAAAGAAATTCCAGTGTC
AATTAAAAAAGAGCCTGAAGTTACTGTAGTTTCACAGCCCACTGAACCTCAGCCTGTTCT
AATACCCAGTATTAATATCAACTCTGACAGTGGAGAAAATAAAGAAGAAATAGGTTCTTT
ATCAAAAACTGAAACTATTCTGCCACCAGAATCTGAGAATCCAAAGGAAAATGATAATGA
TTCAGGCACTGGTTCCACTGCTGATACTAGCAGTATTGACTTGAATTTATCCATCTCTAG
CTTTCTAAGTAAAACTAAAGACAGTGGATCGATATCTTTACAAGAAACAAGAAGACAAAA
GAAAACATTGAAGAAAACACGCAAATTTATTGTTGATGGTGTAGAAGTGAGTGTAACAAC
ATCAAAGATAGTTACAGATAGTGATTCCAAAACTGAAGAATTGCGGTTTCTTAGACGTCA
GGAACTTCGGGAATTAAGATTTCTTCAGAAAGAAGAGCAAAGAGCCCAACAACAGCTCAA
TAGCAAACTACAGCAACAACGAGAACAAATTTTCCGGCGCTTTGAGCAGGAAATGATGAG
TAAAAAGCGACAATATGACCAGGAAATTGAGAATCTAGAAAAACAGCAGAAACAGACTAT
CGAACGCCTGGAACAAGAGCACACAAATCGCTTGCGAGATGAAGCCAAACGCATCAAAGG
AGAACAAGAGAAAGAGTTGTCCAAATTTCAGAATATGCTGAAGAACCGAAAGAAGGAGGT
TATAAATGAAGTGGAGAAAGCACCCAAAGAGCTGAGAAAAGAGCTCATGAAACGCAGGAA
AGAGGAGCTTGCACAAAGCCAGCATGCTCAGGAACAAGAGTTTGTTCAGAAACAACAGCA
AGAATTAGATGGCTCTCTGAAAAAGATCATCCAGCAGCAGAAGGCAGAGTTAGCTAATAT
TGAGAGAGAGTGCCTGAATAACAAGCAACAGCTCATGAGAGCTCGAGAAGCTGCAATTTG
GGAGCTCGAAGAACGACACTTACAAGAAAAACACCAGCTGCTCAAACAGCAGCTTAAAGA
TCAGTATTTCATGCAAAGACATCAGCTACTTAAGCGCCACGAGAAGGAAACAGAGCAAAT
GCAGCGTTACAATCAAAGACTTATTGAGGAATTGAAAAACAGACAGACTCAAGAAAGAGC
AAGACTGCCCAAGATTCAGCGCAGTGAAGCCAAGACTCGAATGGCCATGTTTAAGAAGAG
TTTGAGAATTAACTCAACAGCCACACCAGATCAGGACCGTGATAAAATTAAACAGTTTGC
TGCACAAGAAGAAAAGAGGCAGAAAAATGAGAGAATGGCTCAGCATCAGAAACATGAGAA
TCAAATGCGAGATCTTCAGTTGCAGTGTGAAGCCAATGTCCGCGAACTGCATCAGCTGCA
GAATGAAAAATGCCACTTGTTGGTTGAGCATGAGACTCAGAAACTGAAGGAGTTAGATGA
GGAACATAGCCAAGAATTAAAGGAGTGGAGAGAGAAATTGAGACCTAGGAAAAAGACACT
GGAAGAAGAGTTTGCCAGGAAACTACAGGAACAGGAAGTATTCTTTAAAATGACTGGGGA
GTCTGAATGCCTTAACCCATCAACACAGAGCCGGATTTCCAAATTTTATCCTATTCCCAG
CTTGCATTCCACCGGATCATAACAAAGGGAAGCATTCTGTGCGTGGGTTTGGCTCTTTCA
GTATGTCATTCTGTTCTCATCTTCTGCCACAGTCTCTCAGATAGCTCATGAAGACAATCA
CCTGCCTCACCTTCTAGGTGTTTTCCTTTTTTGTTTTTTTTGTTTTGTTTTGTTTTTAAG
CAAAGATGAAGGGAAAACGAACTAAGACAGACGCTAGGCCATGTTGGCAAAGTAGCATCT
TGGTGACTAAGGTGACTTTGTATATTCATCTTAAAAATTATGTTCTTTAGACACTGCTAC
CTGAAAACTGTTGGAGAAATAATGTTTAAAGTTATTTAAGAAAAACTGTTACATCACTAA
GTATTAATAAATTCTTCTTACCTGACGTAACTTCTCAATGCCTAAATTCTGTAGTTGAAG
CTCTGCTGCAGAGAGTTGGGATAATTTTCTTTTGGTGGATCAGCTCTCATAAAAAAGCTA
TGATTTGCTCAAATATGCTGTTGACTCAGTAAATGAATATATTTTTTTCTTTAAATAGGA
ACAACCTCTTTTAAAAGAGAAAAATTATTTCAGTGATTTGTCAAAACGAATTACCTCTTT
TGGCATGAGCTAATAATTGAGGGTGCTAATTTTCTTAAGATAGTGCCTAAAACACTAAAT
TTCAGTCAAGTCGTAAGTAGGATTTTCTTTTTGATCAACAGGGACAAAAACATCTTTAGA
ATTAAAAACATGGTTGTTTTGGAATTTTTGCTTCTCTTACCGTTTGATAGAAATTTTCAT
CCTAAAATACATGTACAAAGTTTGGAAAGATGAAAAAAAGAGGTAGCTTTTAGATTGCAA
ATTGGAAATGTAAAACTCATGAAATTTAAGCAATATAGGTTTAGCTATCTGTGTTTATTT
TCTAAAATAATACCTGAGCTGGTTAAATGATTTCTCTCCATCTTAGCTAATTCTGTTTAA
AACTCTGTCAGAGGCCTGCAGGCTGTGAGTTATATTTATAAATATATCTTCAGAAATTAA
TCTTAAAAGAGGCATTAGTTCAGAATACTTTTTTAAAAGTTTAAATTAAATATTTAGGCA
CGTCAGAAATTACTTTTCCTTATTTTGAAATGAGGCTACTTATGTCTTGGTTTTATTTTG
TTCCATGTTTAAATCATTCACTTTGATTTGAGTGGGAAAAGCCTGAAGCCTTTATCATGT
GGTTGCTGGTGTGTGTAATTATTAATGAAATGTTCACTCCTAGTCCCTTATGAGGCTTAG
AATTTCAACCACGTGTCAGGTCAGACAGTATTATAAACTGTACTTTGCTGTCTGAGACAG
CACATTTGTGAATGATGCTTGCTGCCTGCCATTTTCAACCTATTCTCTCTTAAGAGTGCT
AGGTACCAAATTGTGAAAGTTTGTTTTCAGTTATATTACTTTTGAGGCTGGTGAAAAATT
TAAATGTAACTTTGTGGGAACACTGATTCATATTTAGAAAATGTAAATGTCTGTAGCACT
TTCTTGCAGTTAATTTGAAAACTTTGGATGCTGAACCTTGTTTGTCAGTGATTTAGATGA
TTTAAAAATGCATGTGTGATTTGAATTTTATAATTGTTTTGACAAGCATAATTTACTTGG
ACAACTTCGTAGGTAGCCTTAACTTCTGGCCAAGTTTGTTTTTTATATAAATATATATAC
ATATATACATATTATGTATGGTTGTAAATTCATACACTTATCACATGAATGTGTTACTGT
ATACAAAACTCTTAATGCTTTATTCTCAAATGCTGGGTTGAAAAATGTTTTGAAAGCCTT
TTAAAATATATATCTTTATAAAGTAATATTCAGGATGATGATAAAAATTGTTTATATTGT
TATGATAAAAATGACAGTATAATGTTAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MSFFNFRKIFKLGSEKKKKQYEHVKRDLNPEDFWEIIGELGDGAFGKVYKAQNKETSVLA AAKVIDTKSEEELEDYMVEIDILASCDHPNIVKLLDAFYYENNLWILIEFCAGGAVDAVM LELERPLTESQIQVVCKQTLDALNYLHDNKIIHRDLKAGNILFTLDGDIKLADFGVSAKN TRTIQRRDSFIGTPYWMAPEVVMCETSKDRPYDYKADVWSLGITLIEMAEIEPPHHELNP MRVLLKIAKSEPPTLAQPSRWSSNFKDFLKKCLEKNVDARWTTSQLLQHPFVTVDSNKPI RELIAEAKAEVTEEVEDGKEEDEEEETENSLPIPASKRASSDLSIASSEEDKLSQNACIL ESVSEKTERSNSEDKLNSKILNEKPTTDEPEKAVEDINEHITDAQLEAMTELHDRTAVIK ENEREKRPKLENLPDTEDQETVDINSVSEGKENNIMITLETNIEHNLKSEEEKDQEKQQM FENKLIKSEEIKDTILQTVDLVSQETGEKEANIQAVDSEVGLTKEDTQEKLGEDDKTQKD VISNTSDVIGTCEAADVAQKVDEDSAEDTQSNDGKEVVEVGQKLINKPMVGPEAGGTKEV PIKEIVEMNEIEEGKNKEQAINSSENIMDINEEPGTTEGEEITESSSTEEMEVRSVVADT DQKALGSEVQDASKVTTQIDKEKKEIPVSIKKEPEVTVVSQPTEPQPVLIPSININSDSG ENKEEIGSLSKTETILPPESENPKENDNDSGTGSTADTSSIDLNLSISSFLSKTKDSGSI SLQETRRQKKTLKKTRKFIVDGVEVSVTTSKIVTDSDSKTEELRFLRRQELRELRFLQKE EQRAQQQLNSKLQQQREQIFRRFEQEMMSKKRQYDQEIENLEKQQKQTIERLEQEHTNRL RDEAKRIKGEQEKELSKFQNMLKNRKKEVINEVEKAPKELRKELMKRRKEELAQSQHAQE QEFVQKQQQELDGSLKKIIQQQKAELANIERECLNNKQQLMRAREAAIWELEERHLQEKH QLLKQQLKDQYFMQRHQLLKRHEKETEQMQRYNQRLIEELKNRQTQERARLPKIQRSEAK TRMAMFKKSLRINSTATPDQDRDKIKQFAAQEEKRQKNERMAQHQKHENQMRDLQLQCEA NVRELHQLQNEKCHLLVEHETQKLKELDEEHSQELKEWREKLRPRKKTLEEEFARKLQEQ EVFFKMTGESECLNPSTQSRISKFYPIPSLHSTGS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
