Gene:
Gene Symbol:
TLK2
Gene Name:
tousled-like kinase 2
Original Clone ID:
HsCD00298877
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618252
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2253nts
Open reading frame: 251 to 2503
GCCCGCGTCGGGCGCCTGGCTCTGTACGCGAGCCCGGGGATCTGCGGCCTTCGTGCCCCC
CCTCCCCCGCCCGCCCTCTCGTGGAGCCCGGCGCCGGCGGCGGCTGCCCGGGCGGGGGGT
TGCGGCGCTCAGGAGAGGCCCCGGCTCCGCCCCGGGCCTGCCCAGGGGGAGAGCGGAGCG
GTCCGCAGCCGGGTCGGGTCGGGGCCCCTCCCGGGAGGAGCGTGGAGCGGCGGCGGCGGC
GGCGGCAGAAATGATGGAAGAATTGCATAGCCTGGACCCACGACGGCAGGAATTATTGGA
GGCCAGGTTTACTGGAGTAGGTGTTAGTAAGGGACCACTTAATAGTGAGTCTTCCAACCA
GAGCTTGTGCAGCGTCGGATCCTTGAGTGATAAAGAAGTAGAGACTCCCGAGAAAAAGCA
GAATGACCAGCGAAATCGGAAAAGAAAAGCTGAACCATATGAAACTAGCCAAGGGAAAGG
CACTCCTAGGGGACATAAAATTAGTGATTACTTTGAGTTTGCTGGGGGAAGCGCGCCAGG
AACCAGCCCTGGCAGAAGTGTTCCACCAGTTGCACGATCCTCACCGCAACATTCCTTATC
CAATCCCTTACCGCGACGAGTAGAACAGCCCCTCTATGGTTTAGATGGCAGTGCTGCAAA
GGAGGCAACGGAGGAGCAGTCTGCTCTGCCAACCCTCATGTCAGTGATGCTAGCAAAACC
TCGGCTTGACACAGAGCAGCTGGCGCAAAGGGGAGCTGGCCTCTGCTTCACTTTTGTTTC
AGCTCAGCAAAACAGTCCCTCATCTACGGGATCTGGCAACACAGAGCATTCCTGCAGCTC
CCAAAAACAGATCTCCATCCAGCACAGACAGACCCAGTCCGACCTCACAATAGAAAAAAT
ATCTGCACTAGAAAACAGTAAGAATTCTGACTTAGAGAAGAAGGAGGGAAGAATAGATGA
TTTATTAAGAGCCAACTGTGATTTGAGACGGCAGATTGATGAACAGCAAAAGATGCTAGA
GAAATACAAGGAACGATTAAATAGATGTGTGACAATGAGCAAGAAACTCCTTATAGAAAA
GTCAAAACAAGAGAAGATGGCGTGTAGAGATAAGAGCATGCAAGACCGCTTGAGACTGGG
CCACTTTACTACTGTCCGACACGGAGCCTCATTTACTGAACAGTGGACAGATGGTTATGC
TTTTCAGAATCTTATCAAGCAACAGGAAAGGATAAATTCACAGAGGGAAGAGATAGAAAG
ACAACGGAAAATGTTAGCAAAGCGGAAACCTCCTGCCATGGGTCAGGCCCCTCCTGCAAC
CAATGAGCAGAAACAGCGGAAAAGCAAGACCAATGGAGCTGAAAATGAAACGTTAACGTT
AGCAGAATACCATGAACAAGAAGAAATCTTCAAACTCAGATTAGGTCATCTTAAAAAGGA
GGAAGCAGAGATCCAGGCAGAGCTGGAGAGACTAGAAAGGGTTAGAAATCTACATATCAG
GGAACTAAAAAGGATACATAATGAAGATAATTCACAATTTAAAGATCATCCAACGCTAAA
TGACAGATATTTGTTGTTACATCTTTTGGGTAGAGGAGGTTTCAGTGAAGTTTACAAGGC
ATTTGATCTAACAGAGCAAAGATACGTAGCTGTGAAAATTCACCAGTTAAATAAAAACTG
GAGAGATGAGAAAAAGGAGAATTACCACAAGCATGCATGTAGGGAATACCGGATTCATAA
AGAGCTGGATCATCCCAGAATAGTTAAGCTGTATGATTACTTTTCACTGGATACTGACTC
GTTTTGTACAGTATTAGAATACTGTGAGGGAAATGATCTGGACTTCTACCTGAAACAGCA
CAAATTAATGTCGGAGAAAGAGGCCCGGTCCATTATCATGCAGATTGTGAATGCTTTAAA
GTACTTAAATGAAATAAAACCTCCCATCATACACTATGACCTCAAACCAGGTAATATTCT
TTTAGTAAATGGTACAGCGTGTGGAGAGATAAAAATTACAGATTTTGGTCTTTCGAAGAT
CATGGATGATGATAGCTACAATTCAGTGGATGGCATGGAGCTAACATCACAAGGTGCTGG
TACTTATTGGTATTTACCACCAGAGTGTTTTGTGGTTGGGAAAGAACCACCAAAGATCTC
AAATAAAGTTGATGTGTGGTCGGTGGGTGTGATCTTCTATCAGTGTCTTTATGGAAGGAA
GCCTTTTGGCCATAACCAGTCTCAGCAAGACATCCTACAAGAGAATACGATTCTTAAAGC
TACTGAAGTGCAGTTCCCGCCAAAGCCAGTAGTAACACCTGAAGCAAAGGCGTTTATTCG
ACGATGCTTGGCCTACCGAAAGGAGGACCGCATTGATGTCCAGCAGCTGGCCTGTGATCC
CTACTTGTTGCCTCACATCCGAAAGTCAGTCTCTACAAGTAGCCCTGCTGGAGCTGCTAT
TGCATCAACCTCTGGGGCGTCCAATAACAGTTCTTCTAATTGAGACTGACTCCAAGGCCA
CAAACTGTTCAACACACACAAAGTGGACAAATGGCGTTCAGCAGCGGGTTTGGAACATAG
CGAATCCGAATGGATCTGATGAAACCTGTACCAGGTGCTTTTATTTTCTTGCTTTTTTCC
CATCCATAGAGCATGACAGCATCGATTCTCATTGAGGAGAAACCTTGGGCAGCTCCGGCC
AGGCCTTGTAGGAAAAGGCCCCGCCCGAGGTTCCAGCGTCAACGGCCACTGTGTGTGGCT
GCTCTGAGTGAGGAAAAAATTAAAAAGAAAAACTGGTTCCATGTACTGTGAACTTGAAAA
CTTGCAGACTCAGGGGGGTCCCTGATGCAGTGCTTCAGATGAAGAATGTGGACTTGAAAA
TACAGACTGGGCTAGTCCAGTGTCTATATTTAAACTTGTTCTTTTCTTTTAATAAAGTTT
AGGTAACATCTCCTGAAAAGCTTGTAGCACAAAGGCTCAGCTGGGGATGGTGTTTGACTT
CGGAGGAAAAAAGTTGCTATTGCCCGTTAAAGGCACTAGAGTTAGTGTTTTATCCCTAAA
TAATTTCAATTTTTAAAAACATGCAGCTTCCCTCTCCCCTTTTTTATTTTTGAAAGAATA
CATTTGGTCATAAAGTGAAACCCGTATTAGCAAGTACGTGGCAATGTTCATTCCAATCAG
ATGCAGCTTTCTCCTCCGTCTGGTCTCCTGTTTGCAATTGCTTCCCTCATCTCAGTAGGG
AAAAAATTGAGTGGGAGTACTGAGATGTGTGGGTTTTTGCCATTGGACAAAGAATGAGGT
TAGAAGACTGCAGCTTGGAGTCTCTCTAGGTTTTCAACTATTTCTTCACAATTTGAACAC
TTGACGGTTGTCCCTTTTAATTTATTTGAAGTGCTATTTTTTTAAATAAAGGTTCATCTG
TCCATGCAGTCCTCTTGGATTCTCTGAATGTAGTAACTATGAAGCTATGCCAAAGGTAGG
CAGAAAATGGGCTTGGGAGCCAGACACTCCAGGGATCAGTAGTTCCTACCCATAGTCTTT
GTGTGCTTGCTAAATGGTGTACATTTCTTGTTAACCACGTTATTAAAACTTGATCAGCAC
ATAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQ RNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSAPGTSPGRSVPPVARSSPQHSLSNPL PRRVEQPLYGLDGSAAKEATEEQSALPTLMSVMLAKPRLDTEQLAQRGAGLCFTFVSAQQ NSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISALENSKNSDLEKKEGRIDDLLR ANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFT TVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQAPPATNEQ KQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELK RIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDE KKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLM SEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDD DSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFG HNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLL PHIRKSVSTSSPAGAAIASTSGASNNSSSN*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
