Gene:
Gene Symbol:
SIK3
Gene Name:
SIK family kinase 3
Original Clone ID:
HsCD00298687
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618254
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
CAGCCGCGTCCCCCAGCCCCGGCCTCCCGCGGACCCATGCCCGCCCGTATCGGCTACTAC
GAGATCGACCGCACCATCGGCAAGGGCAACTTCGCGGTGGTCAAGCGGGCCACGCACCTC
GTCACCAAGGCCAAGGTTGCTATCAAGATCATAGATAAGACCCAGCTGGATGAAGAAAAC
TTGAAGAAGATTTTCCGGGAAGTTCAAATTATGAAGATGCTTTGCCACCCCCATATCATC
AGGCTCTACCAGGTTATGGAGACAGAACGGATGATTTATCTGGTGACAGAATATGCTAGT
GGAGGGGAAATATTTGACCACCTGGTGGCCCATGGTAGAATGGCAGAAAAGGAGGCACGT
CGGAAGTTCAAACAGATCGTCACAGCTGTCTATTTTTGTCACTGTCGGAACATTGTTCAT
CGTGATTTAAAAGCTGAAAATTTACTTCTGGATGCCAATCTGAATATCAAAATAGCAGAT
TTTGGTTTCAGTAACCTCTTCACTCCTGGGCAGCTGCTGAAGACCTGGTGTGGCAGCCCT
CCCTATGCTGCACCTGAACTCTTTGAAGGAAAAGAATATGATGGGCCCAAAGTGGACATC
TGGAGCCTTGGAGTTGTCCTCTACGTGCTTGTGTGCGGTGCCCTGCCATTTGATGGAAGC
ACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGAAAGTTCCGCATCCCATTTTTTATG
TCCACAGAATGTGAGCATTTGATCCGCCATATGTTGGTGTTAGATCCCAATAAGCGCCTC
TCCATGGAGCAGATCTGCAAGCACAAGTGGATGAAGCTAGGGGACGCCGATCCCAACTTT
GACAGGTTAATAGCTGAATGCCAACAACTAAAGGAAGAAAGACAGGTGGACCCCCTGAAT
GAGGATGTCCTCTTGGCCATGGAGGACATGGGACTGGACAAAGAACAGACACTGCAGTCA
TTAAGATCAGATGCCTATGATCACTATAGTGCAATCTACAGCCTGCTGTGTGATCGACAT
AAGAGACATAAAACCCTGCGTCTCGGAGCACTTCCTAGCATGCCCCGAGCCCTGGCCTTT
CAAGCACCAGTCAATATCCAGGCGGAGCAGGCAGGTACTGCTATGAACATCAGCGTTCCC
CAGGTGCAGCTGATCAACCCAGAGAACCAAATTGTGGAGCCGGATGGGACACTGAATTTG
GACAGTGATGAGGGTGAAGAGCCTTCCCCTGAAGCATTGGTGCGCTATTTGTCAATGAGG
AGGCACACAGTGGGTGTGGCTGACCCACGCACGGAAGTTATGGAAGATCTGCAGAAGCTC
CTACCTGGCTTTCCTGGAGTCAACCCCCAGGCTCCATTCCTGCAGGTGGCCCCTAATGTG
AACTTCATGCACAACCTGTTGCCTATGCAAAACTTGCAACCAACCGGGCAACTTGAGTAC
AAGGAGCAGTCTCTCCTACAGCCGCCCACGCTACAGCTGTTGAATGGAATGGGCCCCCTT
GGCCGGAGGGCATCAGATGGAGGAGCCAACATCCAACTGCATGCCCAGCAGCTGCTGAAG
CGCCCACGGGGACCCTCTCCGCTTGTCACCATGACACCAGCAGTGCCAGCAGTTACCCCT
GTGGACGAGGAGAGCTCAGACGGGGAGCCAGACCAGGAAGCTGTGCAGAGCTCTACCTAC
AAGGACTCCAACACTCTGCACCTCCCTACGGAGCGTTTCTCCCCTGTGCGCCGGTTCTCA
GATGGGGCTGCGAGCATCCAGGCCTTCAAAGCTCACCTGGAAAAAATGGGCAACAACAGC
AGCATCAAACAGCTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCAGATT
GATGAAAGAACCCTGGAGAAGACCCAGCAGCAGCATATGTTATACCAGCAGGAGCAGCAC
CATCAAATTCTCCAGCAACAAATTCAAGACTCTATCTGTCCTCCTCAGCCATCTCCACCT
CTTCAGGCTGCATGTGAAAATCAGCCAGCCCTCCTTACCCATCAGCTCCAGAGGTTAAGG
ATTCAGCCTTCAAGCCCACCCCCCAACCACCCCAACAACCATCTCTTCAGGCAGCCCAGT
AATAGTCCTCCCCCCATGAGCAGTGCCATGATCCAGCCTCACGGGGCTGCATCTTCTTCC
CAGTTTCAAGGCTTACCTTCCCGCAGTGCAATCTTTCAGCAGCAACCTGAGAACTGTTCC
TCTCCTCCCAACGTGGCACTAACCTGCTTGGGTATGCAGCAGCCTGCTCAGTCACAGCAG
GTCACCATCCAAGTCCAAGAGCCTGTTGACATGCTCAGCAACATGCCAGGCACAGCTGCA
GGCTCCAGTGGGCGCGGCATCTCCATCAGCCCCAGTGCTGGTCAGATGCAGATGCAGCAC
CGTACCAACCTGATGGCCACCCTCAGCTATGGGCACCGTCCCTTGTCCAAGCAGCTGAGT
GCTGACAGTGCAGAGGCTCACAGCTTGAACGTGAATCGGTTCTCCCCTGCTAACTACGAC
CAGGCGCATTTACACCCCCATCTGTTTTCGGACCAGTCCCGGGGTTCCCCCAGCAGCTAC
AGCCCTTCAACAGGAGTGGGGTTCTCTCCAACCCAAGCCCTGAAAGTCCCTCCACTTGAC
CAATTCCCCACCTTCCCTCCCAGTGCACATCAGCAGCCGCCACACTATACCACGTCGGCA
CTACAGCAGGCCCTGCTGTCTCCCACGCCGCCAGACTATACAAGACACCAGCAGGTACCC
CACATCCTTCAAGGACTGCTTTCTCCCCGGCATTCGCTCACCGGCCACTCGGACATCCGG
CTGCCCCCAACAGAGTTTGCACAGCTCATTAAAAGGCAGCAGCAACAACGGCAGCAGCAG
CAGCAACAGCAGCAACAGCAAGAATACCAGGAACTGTTCAGGCACATGAACCAAGGGGAT
GCGGGGAGTCTGGCTCCCAGCCTTGGGGGACAGAGCATGACAGAGCGCCAGGCTTTATCT
TATCAAAATGCTGACTCTTATCACCATCACACCAGCCCCCAGCATCTGCTACAAATCAGG
GCACAAGAATGTGTCTCACAGGCTTCCTCACCCACCCCGCCCCACGGGTATGCTCACCAG
CCGGCACTGATGCATTCAGAGAGCATGGAGGAGGACTGCTCGTGTGAGGGGGCCAAGGAT
GGCTTCCAAGACAGTAAGAGTTCAAGTACATTGACCAAAGGTTGCCATGACAGCCCTCTG
CTCTTGAGTACCGGTGGACCTGGGGACCCTGAATCTTTGCTAGGAACTGTGAGTCATGCC
CAAGAATTGGGGATACATCCCTATGGTCATCAGCCAACTGCTGCATTCAGTAAAAATAAG
GTGCCCAGCAGAGAGCCTGTCATAGGGAACTGCATGGATAGAAGTTCTCCAGGACAAGCA
GTGGAGCTGCCGGATCACAATGGGCTCGGGTACCCAGCACGCCCCTCCGTCCATGAGCAC
CACAGGCCCCGGGCCCTCCAGAGACACCACACGATCCAGAACAGCGACGATGCTTATGTA
CAGCTGGATAACTTGCCAGGAATGAGTCTCGTGGCTGGGAAAGCACTTAGCTCTGCCCGG
ATGTCGGATGCAGTTCTCAGTCAGTCTTCGCTCATGGGCAGCCAGCAGTTTCAGGATGGG
GAAAATGAGGAATGTGGGGCAAGCCTGGGAGGTCATGAGCACCCAGACCTGAGTGATGGC
AGCCAGCATTTAAACTCCTCTTGCTATCCATCTACGTGTATTACAGACATTCTGCTCAGC
TACAAGCACCCCGAAGTCTCCTTCAGCATGGAGCAGGCAGGCGTGTAACAAGAAACAGAG
AGTTTTGTGTACAGCTTGGGAATGAAAAGGTTGATTGTAAACCCACAGTATCTAGCAGCG
TTGTGCCAAATTGCCCTTGTGTTTCTCTCCACCCAAAATATCACAGCTGCTTTCCTCACA
TTTGGTTCATCCGTGTGCTGTTCTTTTGGGTTCTGAGAGGGTTTTGCCATGTTTGCTTGT
ATGACCAAGTCACCAAGGAAATAAACAGGAAGGAAATCCATGTTCTCCATCTTTTGTGAA
AGTATATTTGAGTTGGTGGTTTTTTGTTTTGTTTGGGGGTTTGTGTTTTGTTTTGTTTTT
GGTATGTTTTCTTCCAGAGGTGATATACTTTCTTTTTTTTCTTCCTTTCTTTTTTTTCTT
TCGTTCCTTTTTTGAAACAGGAGAGCAAAGCAGTTAGAGTTCAGAGGCCAGCGGCCTCAG
GGCCACTCCCTCCCTAGCCTTCATCAGCAGAGCACCCTCCATCCCCCTGCATTGCTCTTC
TGTGAAAGCAAATACTAAAGGATGCCATCCTCTGGAATCCTAATGGCAGGCAAAGGGAGA
GAGGAAGGGTGACGGCTTCTGGCACTTAGAAAACAAAAAGAACAAAAAAAGAGAAACCCC
CAAGCCTGGAACGCAGAGAGGTCTTTACTGCTGGGATCCACGGAAAACATGTCTGTCCTA
GCCAAGATCATATGAAGAGTTTGGCACGGAGGCTGAGAATGACCTGGCATAGATGGTTTG
CCAGTTAGGATGTCTCAATTTGAGCCTTTGCTTTTGGTGGATAACTCAGCTCCCCTCTTG
TAACCTGGAAAGTTGGTTGCCTTTATCATCCTGCTGGTTTTATCCATGGACTGAACACCC
AACAGCAGTGCACTATGCTTTCTATGGCATCTTTCATTCTCATTTTATATTGTGCTATAA
AAAGGATTGTTTCTCCATATATATATTATATATGTGTGTATATATATAATATAATATATG
TGTATATATATATTATATATATAATATATAATATAATATATTATATATATATTATATATA
TAATATATATATAAAATATATATATATATGCTCTCCTCTTTCAGCCTCTTTGTCACAGGG
AAGAAGTGTAGGAGGTTGCCTTGGGCCCTGCCTCTCTCCTAACCTCCTCTTCCCCACTGG
GTACCCTCAGCCCCTATATTTTAATTCTTGATCATGTAGAAATTGTTTTTGGTAAATGTT
GATATTATTGTTATTATCATTATTAATAAATAAAGAGAAAAGGAATTTTTGTTTAAATGA
GAAATGTTTAACCAGATTCTGTTCTATTTGAATTGTGACTTGCACCTTTTGTTCAAAGTA
TTTCCTTTAGGCATTGTAATTGTGAACAGCTCTTACTTGTGCCAGTGACAGATGCAGTGG
TCTCCTTTCCCCAGTTGAAGCAGTGCATACGCAGTAGCTATTATTTGTGTTATCTTTATT
TCTCTTCATTGTTAGAAACCAAAGTCTTCTCTGCTGGCTGGGGCTGAGAGAGGGTCTGGG
TTATCTCCTTCTGATCTTCAAAACAAGAGAGAGACCTTGAATACACTGACTCTTCCACCC
TTTTTTTTTCTGGGAAAGGAGAGCAAGAGGTCCCGAGTCCCCTCCTAGTCTTTCATCCTG
AATTTGCACAGAGGAAAGCGGGTGCCCGGCATGGCCATCCTGATGTTGCTGGCGGGATCC
CCATGCACCTTGTCCTTCTCCACTGATACTGGCAGCTCGGCTCCTGGACCCAAGATCCCT
TGAGTGGAATTCTGCAGTGCAAGAGCCCTTCGTGGGAGCTGTCCCATGTTTCCATGGTCC
CCAGTCTCCCCTCCACTTGGTGGGGTCACCAACTACTCACCAGAAGGGGGCTTACCAAGA
AAGCCCTAAAAAGCTGTTGACTTATCTGCGCTTGTTCCAACTCTTATGCCCCCAACCTGC
CCTACCACCACCACGCGCTCAGCCTGATGTGTTTACATGGTACTGTATGTATGGGAGAGC
AGACTGCACCCTCCAGCAACAACAGATGAAAGCCAGTGAGCCTACTAACCGTGCCATCTT
GCAAACTACACTTTAAAAAAAACTCATTGCTTTGTATTGTAGTAACCAATATGTGCAGTA
TACGTTGAATGTATATGAACATACTTTCCTATTTCTGTTCTTTGAAAATGTCAGAAATAT
TTTTTTCTTTCTCATTTTATGTTGAACTAAAAAGGATTAAAAAAAAAATCTCCAGACTCA
AGTTGCTAAAAA
Protein sequence predicted based on nucleotide sequence
MPARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMK MLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYF CHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKE YDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHML VLDPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKEERQVDPLNEDVLLAMEDMGL DKEQTLQSLRSDAYDHYSAIYSLLCDRHKRHKTLRLGALPSMPRALAFQAPVNIQAEQAG TAMNISVPQVQLINPENQIVEPDGTLNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTE VMEDLQKLLPGFPGVNPQAPFLQVAPNVNFMHNLLPMQNLQPTGQLEYKEQSLLQPPTLQ LLNGMGPLGRRASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQ EAVQSSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMGNNSSIKQLQQECEQL QKMYGGQIDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQAACENQPALL THQLQRLRIQPSSPPPNHPNNHLFRQPSNSPPPMSSAMIQPHGAASSSQFQGLPSRSAIF QQQPENCSSPPNVALTCLGMQQPAQSQQVTIQVQEPVDMLSNMPGTAAGSSGRGISISPS AGQMQMQHRTNLMATLSYGHRPLSKQLSADSAEAHSLNVNRFSPANYDQAHLHPHLFSDQ SRGSPSSYSPSTGVGFSPTQALKVPPLDQFPTFPPSAHQQPPHYTTSALQQALLSPTPPD YTRHQQVPHILQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQQQRQQQQQQQQQQEYQEL FRHMNQGDAGSLAPSLGGQSMTERQALSYQNADSYHHHTSPQHLLQIRAQECVSQASSPT PPHGYAHQPALMHSESMEEDCSCEGAKDGFQDSKSSSTLTKGCHDSPLLLSTGGPGDPES LLGTVSHAQELGIHPYGHQPTAAFSKNKVPSREPVIGNCMDRSSPGQAVELPDHNGLGYP ARPSVHEHHRPRALQRHHTIQNSDDAYVQLDNLPGMSLVAGKALSSARMSDAVLSQSSLM GSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCITDILLSYKHPEVSFSMEQ AGV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
