Gene:
Gene Symbol:
GSG2
Gene Name:
germ cell associated 2 (haspin)
Original Clone ID:
FLH232440.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618276
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2397nts
Open reading frame: 15 to 2411
GGCGGGTGCCGGCCATGGCGGCTTCGCTCCCGGGACCTGGGAGCCGGCTTTTCCGCACAT
ATGGGGCTGCGGACGGCAGGAGACAGCGGCGGCCGGGCCGGGAAGCCGCGCAGTGGTTCC
CGCCGCAGGACCGGAGGCGTTTCTTCAACAGCAGCGGCAGCAGCGACGCCAGCATCGGCG
ACCCCTCGCAGTCCGACGATCCTGACGATCCCGACGACCCCGACTTCCCCGGCAGCCCGG
TGAGGCGGCGGCGGAGGTGTCCCGGCGGCCGAGTGCCCAAGGACCGGCCCAGCCTGACCG
TGACCCCAAAGCGCTGGAAGCTGCGAGCTCGCCCAAGCCTAACCGTGACCCCAAGACGCC
TGGGGCTGCGAGCTCGGCCCCCGCAGAAGTGCAGCACACCCTGCGGCCCGCTCCGACTTC
CGCCCTTCCCCAGCCGCGACTCCGGCCGCCTCAGCCCGGACCTCAGCGTGTGCGGCCAGC
CCAGGGACGGCGACGAGCTGGGCATCAGTGCCTCCCTGTTCAGCTCTCTGGCCTCGCCCT
GCCCCGGGTCCCCAACGCCAAGGGACAGTGTCATCTCGATCGGCACCTCCGCCTGTCTGG
TTGCAGCCTCAGCCGTCCCGAGCGACCTCCACCTCCCAGAAGTCTCCCTGGACCGAGCAT
CTCTCCCCTGCTCCCAGGAGGAAGCGACAGGAGGAGCCAAGGACACCAGGATGGTCCACC
AAACCCGCGCCAGCCTCAGGTCAGTTCTCTTTGGCCTTATGAACTCAGGAACCCCTGAGG
ATTCTGAGTTTCGGGCAGATGGGAAGAATATGAGAGAGTCCTGCTGTAAAAGGAAACTGG
TGGTGGGAAATGGACCAGAGGGTCCAGGTCTGTCAAGCACAGGCAAGAGGAGGGCCACAG
GCCAGGACTCTTGTCAAGAGAGAGGGCTTCAAGAGGCCGTCCGGAGAGAGCATCAGGAGG
CCAGTGTTCCCAAGGGCCGCATTGTGCCAAGGGGAACAGACAGGCTGGAGAGAACTAGAT
CAAGCCGGGAGAGCAAACATCAGGAGGCAACGGAAACCTCTCTCCTCCATTCCCACCGCT
TTAAAAAGGGCCAAAAGCTGGGAAAAGATTCGTTCCCCACCCAGGACCTGACTCCTTTAC
AGAATGCCTGCTTTTGGACCAAAACCAGGGCTTCCTTCAGTTTCCACAAGAAGAAAATTG
TGACTGATGTGTCAGAGGTCTGCAGCATCTATACCACTGCCACTTCTCTCTCTGGATCCC
TCCTATCAGAATGTTCAAACCGGCCTGTCATGAACAGAACAAGTGGTGCTCCGTCCTCTT
GGCACTCCTCCTCTATGTATTTGCTAAGCCCCTTAAACACTCTAAGTATTTCAAACAAAA
AGGCATCTGATGCTGAAAAGGTTTATGGGGAATGCAGTCAGAAGGGTCCTGTCCCCTTTA
GCCATTGCCTTCCCACAGAAAAACTGCAACGCTGTGAGAAGATTGGGGAAGGGGTGTTTG
GCGAAGTGTTTCAAACAATTGCTGATCACACACCCGTAGCCATAAAAATCATTGCTATTG
AAGGACCAGATTTAGTCAATGGATCCCATCAGAAAACCTTTGAGGAAATCCTGCCAGAGA
TCATCATCTCCAAAGAGTTGAGCCTCTTATCCGGTGAAGTGTGCAACCGCACAGAAGGCT
TTATCGGGCTGAACTCAGTGCACTGTGTCCAGGGATCTTACCCTCCCTTGCTCCTCAAAG
CCTGGGATCACTATAATTCAACCAAAGGCTCTGCAAATGACCGGCCTGATTTTTTTAAAG
ACGACCAGCTCTTCATTGTGCTGGAATTTGAGTTTGGAGGGATTGACTTAGAGCAAATGC
GAACCAAGTTGTCTTCCTTGGCTACTGCAAAGAGCATTCTACACCAGCTCACAGCCTCCC
TCGCAGTGGCAGAGGCATCACTGCGCTTTGAGCACCGAGACTTACACTGGGGGAACGTGC
TCTTAAAGAAAACCAGCCTCAAAAAACTCCACTACACCCTCAATGGGAAGAGCAGCACTA
TCCCCAGCTGTGGGTTGCAAGTGAGCATCATTGACTACACCCTGTCGCGCTTGGAACGGG
ATGGGATTGTGGTTTTCTGTGACGTTTCCATGGATGAGGACCTGTTTACCGGTGACGGTG
ACTACCAGTTTGACATCTACAGGCTCATGAAGAAGGAGAATAACAACCGCTGGGGTGAAT
ATCACCCTTATAGTAATGTGCTCTGGTTACATTACCTGACAGACAAGATGCTGAAACAAA
TGACCTTCAAGACTAAATGTAACACTCCTGCCATGAAGCAAATTAAGAGAAAAATCCAGG
AGTTCCACAGGACAATGCTGAACTTCAGCTCTGCCACTGACTTGCTCTGCCAGCACAGTC
TGTTTAAGTAAGCTAAATGTATCTTACTGCCCCGAAATGAGAGGAGACTGGTCTTGAAGC
CTCTGGTGCTGTTTCAACCTCCGTCCCCACAGGAGGGTGGAACTCCCATTCTCACAGGTT
TCCAGTCAGCTTTTCAAACAAGAATTTTGTTTCCAAATGGAAACTGAAATATTTGTTGAA
ATGTTTAAATTTGCTGATAACAAATGTTCTGAAAGAAGTAAACTAGCCGGGCGCAGTGGC
GTGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGGATCGCTTGAGCCCAAGA
GTTCATATCTAGCCTGGTCAACATAGCAAGACCCCTGTCTCTATTTTTTTAAATAAATAA
ACTACATGTGAAAACAGAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MAASLPGPGSRLFRTYGAADGRRQRRPGREAAQWFPPQDRRRFFNSSGSSDASIGDPSQS DDPDDPDDPDFPGSPVRRRRRCPGGRVPKDRPSLTVTPKRWKLRARPSLTVTPRRLGLRA RPPQKCSTPCGPLRLPPFPSRDSGRLSPDLSVCGQPRDGDELGISASLFSSLASPCPGSP TPRDSVISIGTSACLVAASAVPSDLHLPEVSLDRASLPCSQEEATGGAKDTRMVHQTRAS LRSVLFGLMNSGTPEDSEFRADGKNMRESCCKRKLVVGNGPEGPGLSSTGKRRATGQDSC QERGLQEAVRREHQEASVPKGRIVPRGTDRLERTRSSRESKHQEATETSLLHSHRFKKGQ KLGKDSFPTQDLTPLQNACFWTKTRASFSFHKKKIVTDVSEVCSIYTTATSLSGSLLSEC SNRPVMNRTSGAPSSWHSSSMYLLSPLNTLSISNKKASDAEKVYGECSQKGPVPFSHCLP TEKLQRCEKIGEGVFGEVFQTIADHTPVAIKIIAIEGPDLVNGSHQKTFEEILPEIIISK ELSLLSGEVCNRTEGFIGLNSVHCVQGSYPPLLLKAWDHYNSTKGSANDRPDFFKDDQLF IVLEFEFGGIDLEQMRTKLSSLATAKSILHQLTASLAVAEASLRFEHRDLHWGNVLLKKT SLKKLHYTLNGKSSTIPSCGLQVSIIDYTLSRLERDGIVVFCDVSMDEDLFTGDGDYQFD IYRLMKKENNNRWGEYHPYSNVLWLHYLTDKMLKQMTFKTKCNTPAMKQIKRKIQEFHRT MLNFSSATDLLCQHSLFK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
