Gene:
Gene Symbol:
TRIB1
Gene Name:
tribbles pseudokinase 1
Original Clone ID:
HsCD00298652
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618304
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1119nts
Open reading frame: 583 to 1701
AAGGAGGAGTGCACTGGCCGGGATCGGTGCAGCGCTCACACTCACTCACAGTCACTCTCT
CTGAGCGCGTCTCGCTCGCTCTCATACACGCCCGGAGCCCAGGAGCGCTCAGGATCCCGA
GCGCCGCGAAAAAGTTCCCCCGGCTTTTGCTGGAGACTCATCGTTTTGGGAAGTGCATTT
GCTTCGTGGCTCCGCCGAGCCTGCTGAATCCTGTCCTCGCGGCACGGGACCCCGGGATCG
CTGACCGCTGCCGCCGCCGCCTCTGCCTCCCGGACTATCGGCAGCCTCGGCAACAATAGT
GGCGGCCGCCCCCAGCGAGGCTCCGGGAGCCCTTGCCTGCGGGGGTCCGGGGACTCGAGC
CGGCCTCCGCCTCCCGGACGCACAGCCAGCGTGGTCCCCGCGTGCAACGCGAGCGCCGGG
GAGTGGCTCCTGCTTTGCCCCTCGTGGGGGCCGAGCCAAGACCAGTCTGCAAACTCCATC
CCGCCGGCTGGAAGAAGTCGCGGAGCCGGCACCAAACCCGCAGCGTCTTCCCGCGCGGAT
CCCGGGACTTAAAAAGCCGGGGCCACCCCGGCCCAGGACGGGATGCGGGTCGGTCCGGTG
CGCTCTGCCATGAGCGGCGCCTCGCAGCCCCGCGGCCCGGCCCTGCTCTTCCCAGCCACC
CGAGGCGTCCCGGCCAAACGCCTGCTGGACGCCGACGACGCGGCGGCTGTGGCGGCCAAG
TGCCCGCGCCTCTCCGAGTGCTCCAGCCCCCCGGACTACCTCAGCCCCCCCGGCTCGCCC
TGCAGCCCGCAGCCCCCGCCTGCCGCTCCGGGGGCCGGCGGAGGCTCCGGGAGCGCGCCG
GGGCCCAGCCGCATCGCCGACTACCTGCTGCTGCCCCTAGCCGAGCGCGAGCATGTGTCC
CGGGCGCTGTGCATCCACACTGGACGCGAGCTGCGCTGCAAGGTGTTTCCCATTAAACAC
TACCAGGACAAAATCAGGCCTTACATCCAGCTGCCATCGCACAGCAACATTACTGGCATT
GTGGAAGTGATCCTTGGGGAAACCAAGGCCTATGTCTTCTTTGAGAAGGACTTTGGGGAC
ATGCACTCCTATGTGCGAAGCCGGAAGAGGCTGCGGGAAGAGGAAGCCGCCCGGCTCTTC
AAGCAGATTGTCTCCGCCGTCGCCCACTGCCACCAGTCAGCCATCGTGCTGGGGGACCTG
AAGCTTAGGAAGTTCGTCTTCTCCACGGAGGAGAGAACCCAGCTTAGACTAGAAAGTCTA
GAAGACACACACATAATGAAGGGGGAAGATGATGCTTTGTCAGACAAACATGGCTGCCCA
GCCTACGTGAGCCCTGAGATCCTCAACACCACTGGGACCTACTCCGGAAAGGCTGCGGAC
GTTTGGAGCCTGGGGGTGATGCTCTACACCCTTCTGGTTGGACGATACCCCTTCCATGAC
TCAGACCCCAGTGCCCTTTTCTCCAAAATTCGGCGTGGACAGTTCTGCATTCCTGAGCAC
ATTTCCCCCAAAGCCAGGTGCCTCATTCGCAGCCTCTTGAGACGGGAGCCCTCCGAGAGA
CTCACTGCCCCCGAGATCCTACTGCACCCCTGGTTTGAGTCCGTCTTGGAACCCGGGTAC
ATCGACTCAGAAATAGGAACTTCAGACCAGATTGTTCCAGAGTACCAGGAGGACAGTGAC
ATTAGTTCCTTCTTCTGCTAATCCCCAAAACCTCAGAAACCTCATAATTCTTAACACCTG
GCATTTCCATTTCTAAAGATGGACAGGCCCTTTGGCGTGGTACCAACCAGATAATGACTG
CATCAGGATGAAAGCTGCTGAACTCGGCATGGCGCCTCCTCTTCTCTGTTGGGATGAGTG
ACTTTATTGATTTGAGCAGCATATGCTGTGATTGGCTGCCCTGCAAATTTGTTTCCCTTA
AGGAACCCTCACCAACTATCTCTGCTGGATTTGGGAGTTCCGCATCTTTTGTGGAGGGCA
GAGTATGGACATCTTACACCCGGTGGTCAAGTGTGTAATAAACTTGAGCATTCGAATGGG
AGAAAAAGCAAATCGCACAATGACATATTTTGAGTAATAACCGTATTTTTCACAGGGTGA
CAAATTGGGCCAATAAATCTGCCATCTTTGAACTCATCTTTGGTGGCTAGACTGCTACGG
CAGCTTCTCTGATGGGAAAGTTCCTTTTTTGGCTTAACACTCACCCTTTCTTCACACTCA
CATTTACCAATGACTCTGCTCCGTTTTTGGAGCAGACTGTTTTAAGTTGCTCAGGAGCCT
GATGGAACCATGAACCGAGACTCTTCTCTGTTTCCTGCCAAGACCTCATCTGCACTAATG
CCTTCTCCCTGACCTTGACACTTCCCCCTTTAGCTATAAAAGCACTTACCAGCCGAACGT
GGAACAGTATCACAAAAGATTCCATCTCCCAACGATTTCAGAACTCTGAGCTCAGAGAGA
CTCCAGATTTTAAAAAATAATTTGAGTGCTTGGAAACTATTAGCTTTTTAAGTTCCTTCC
AAATATGTTAGTACCTACCCTTTACTTTTTCCCCAAGACCATCTCAGGGTGGAGCATTCT
GTCTAAGAGAAGAAAGATAAGGAGGCTCCCACCCACCTCTCCCAAGAGCAGACATTAAAC
ATCTTTGTGCTTTGAAGAGAGTGAATTTTGGATAGTCTTGTGATTCTCAGACTAACTTCC
AGAATTATACTTTAACCCCTCCCAGATATGGTCCGCCTTTGGCATTGTGTGTACATCTGC
AGTTTTGCATGGTGGGTTGTTAATATTTCAAATGTGTGGTTTATGAATACGTCTGTATAA
TCGGCTTCTGGAGTGAAACAGCAAACCCCAAATCTTCAAAGTTGGAAGGAACTTTAAAAA
TCATCCGGTCCAATCTCTTTCCTCTTTCTGCCACCTCCCAAGGCAGAAATCCCCTCTTCA
GCTTCTTTTGTAGGTGGGAATCCAGCCTCTGTTAGATATGTCCAGAGATGGAAACTCACT
CCCCTACAAAAGATGGAGCTTAATGGAGAAATTGCAACTTTCATTAAAAAACAAATTCAG
ATGAAATATCAGTAACTGTCTTGGACAGTGCTGAAATCAGGTGGTTAAACGGGTAAACAA
AATATACTGTATTTTGAGAAATGGCACAAAAACAGGCAGTCATCTTTAAGGGCTATGCCT
AGGCAAACTACTAACATGCATTGTGAGAATGCCGTGTATACCTCACGTACTGTGTACTTT
GTACATATATTTTACCTTTTATACCTATGTTCGATTTTGTTTTGTTTTGTTTTGTTCTGG
CTTTGAGGCTTGTTTTGTTGTCTGTGTCTGTCTGAATAACCTGCGTGTCTAAAACCACGT
GAAATGTGAATGATTATTGGCAATATTACCTTGACAGAATCATGGGACTTTGAGAAGAGG
GAGGACAGAGGCCTCTGTCGCACTAACGCTCTCGTGGTTGCTCGACTGTTGTATCTGTGA
TACATTATCCGACTAAGGACTCTGGGCTGGCAGGGCCTTCTGCCGGGAAAGCTAGAAACA
CTAGGTTCTTCCTGTACATACGTGTATATATGTGAACAGTGAGATGGCCGTTTCTGACTT
GTAGAGAAATTTTAATAAACCTGGTTTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MRVGPVRSAMSGASQPRGPALLFPATRGVPAKRLLDADDAAAVAAKCPRLSECSSPPDYL SPPGSPCSPQPPPAAPGAGGGSGSAPGPSRIADYLLLPLAEREHVSRALCIHTGRELRCK VFPIKHYQDKIRPYIQLPSHSNITGIVEVILGETKAYVFFEKDFGDMHSYVRSRKRLREE EAARLFKQIVSAVAHCHQSAIVLGDLKLRKFVFSTEERTQLRLESLEDTHIMKGEDDALS DKHGCPAYVSPEILNTTGTYSGKAADVWSLGVMLYTLLVGRYPFHDSDPSALFSKIRRGQ FCIPEHISPKARCLIRSLLRREPSERLTAPEILLHPWFESVLEPGYIDSEIGTSDQIVPE YQEDSDISSFFC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
