Gene:
Gene Symbol:
SIK1
Gene Name:
salt-inducible kinase 1
Original Clone ID:
HsCD00298659
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618307
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2352nts
Open reading frame: 134 to 2485
ATGCGGCGCGGCCCCGGAGGCAGCAGCAGCGGCGGCGGCAGCCGGAGCAGTAGGCACCCG
AGCAGCGCCAGCGGCCGAGCGGGCGGCTTCCTGGCCTGGGCGCTCCGGTGGCGGCGGAGG
TGCGCGCGGAGCCATGGTTATCATGTCGGAGTTCAGCGCGGACCCCGCGGGCCAGGGTCA
GGGCCAGCAGAAGCCCCTCCGGGTGGGTTTTTACGACATCGAGCGGACCCTGGGCAAAGG
CAACTTCGCGGTGGTGAAGCTGGCGCGGCATCGAGTCACCAAAACGCAGGTTGCAATAAA
AATAATTGATAAAACACGATTAGATTCAAGCAATTTGGAGAAAATCTATCGTGAGGTTCA
GCTGATGAAGCTTCTGAACCATCCACACATCATAAAGCTTTACCAGGTTATGGAAACAAA
GGACATGCTTTACATCGTCACTGAATTTGCTAAAAATGGAGAAATGTTTGATTATTTGAC
TTCCAACGGGCACCTGAGTGAGAACGAGGCGCGGAAGAAGTTCTGGCAAATCCTGTCGGC
CGTGGAGTACTGTCACGACCATCACATCGTCCACCGGGACCTCAAGACCGAGAACCTCCT
GCTGGATGGCAACATGGACATCAAGCTGGCAGATTTTGGATTTGGGAATTTCTACAAGTC
AGGAGAGCCTCTGTCCACGTGGTGTGGGAGCCCCCCGTATGCCGCCCCGGAAGTCTTTGA
GGGGAAGGAGTATGAAGGCCCCCAGCTGGACATCTGGAGCCTGGGCGTGGTGCTGTACGT
CCTGGTCTGCGGTTCTCTCCCCTTCGATGGGCCTAACCTGCCGACGCTGAGACAGCGGGT
GCTGGAGGGCCGCTTCCGCATCCCCTTCTTCATGTCTCAAGACTGTGAGAGCCTGATCCG
CCGCATGCTGGTGGTGGACCCCGCCAGGCGCATCACCATCGCCCAGATCCGGCAGCACCG
GTGGATGCGGGCTGAGCCCTGCTTGCCGGGACCCGCCTGCCCCGCCTTCTCCGCACACAG
CTACACCTCCAACCTGGGCGACTACGATGAGCAGGCGCTGGGTATCATGCAGACCCTGGG
CGTGGACCGGCAGAGGACGGTGGAGTCACTGCAAAACAGCAGCTATAACCACTTTGCTGC
CATTTATTACCTCCTCCTTGAGCGGCTCAAGGAGTATCGGAATGCCCAGTGCGCCCGCCC
CGGGCCTGCCAGGCAGCCGCGGCCTCGGAGCTCGGACCTCAGTGGTTTGGAGGTGCCTCA
GGAAGGTCTTTCCACCGACCCTTTCCGACCTGCCTTGCTGTGCCCGCAGCCGCAGACCTT
GGTGCAGTCCGTCCTCCAGGCCGAGATGGACTGTGAGCTCCAGAGCTCGCTGCAGTGGCC
CTTGTTCTTCCCGGTGGATGCCAGCTGCAGCGGAGTGTTCCGGCCCCGGCCCGTGTCCCC
AAGCAGCCTGCTGGACACAGCCATCAGTGAGGAGGCCAGGCAGGGGCCGGGCCTAGAGGA
GGAGCAGGACACGCAGGAGTCCCTGCCCAGCAGCACGGGCCGGAGGCACACCCTGGCCGA
GGTCTCCACCCGCCTCTCCCCACTCACCGCGCCATGTATAGTCGTCTCCCCCTCCACCAC
GGCAAGTCCTGCAGAGGGAACCAGCTCTGACAGTTGTCTGACCTTCTCTGCGAGCAAAAG
CCCCGCGGGGCTCAGTGGCACCCCGGCCACTCAGGGGCTGCTGGGCGCCTGCTCCCCGGT
CAGGCTGGCCTCGCCCTTCCTGGGGTCGCAGTCCGCCACCCCAGTGCTGCAGGCTCAGGG
GGGCTTGGGAGGAGCTGTTCTGCTCCCTGTCAGCTTCCAGGAGGGACGGCGGGCGTCGGA
CACCTCACTGACTCAAGGGCTGAAGGCCTTTCGGCAGCAGCTGAGGAAGACCACGCGGAC
CAAAGGGTTTCTGGGACTGAACAAAATCAAGGGGCTGGCTCGCCAGGTGTGCCAGGTCCC
TGCCAGCCGGGCCAGCAGGGGCGGCCTGAGCCCCTTCCACGCCCCTGCACAGAGCCCAGG
CCTGCACGGCGGCGCAGCCGGCAGCCGGGAGGGCTGGAGCCTGCTGGAGGAGGTGCTAGA
GCAGCAGAGGCTGCTCCAGTTACAGCACCACCCGGCCGCTGCACCCGGCTGCTCCCAGGC
CCCCCAGCCGGCCCCTGCCCCGTTTGTGATCGCCCCCTGTGATGGCCCTGGGGCTGCCCC
GCTCCCCAGCACCCTCCTCACGTCGGGGCTCCCGCTGCTGCCGCCCCCACTCCTGCAGAC
CGGCGCGTCCCCGGTGGCCTCAGCGGCGCAGCTCCTGGACACACACCTGCACATTGGCAC
CGGCCCCACCGCCCTCCCCGCTGTGCCCCCACCACGCCTGGCCAGGCTGGCCCCAGGTTG
TGAGCCCCTGGGGCTGCTGCAGGGGGACTGTGAGATGGAGGACCTGATGCCCTGCTCCCT
AGGCACGTTTGTCCTGGTGCAGTGAGGGCAGCCCTGCATCCTGGCACGGACACTGACTCT
TACAGCAATAACTTCAGAGGAGGTGAAGACATCTGGCCTCAAAGCCAAGAACTTTCTAGA
AGCGAAATAAGCAATACGTTAGGTGTTTTGGCTTTTTAGTTTATTTTTGTTTTATTTTTT
TCTTGCACTGAGTGACCTCAACTTTGAGTAGGGACTGGAAACTTTAGGAAGAAAGATAAT
TGAGGGGCGTGTCTGGGGGCGGGGGCAGGAGGGGAGCGGGGTGGAGGGAACACGTGCAGT
GCCGTGGTGTGGGGATCTCGGCCCCTCTCTCTGGGTTCGTCGTGGTTGAGATGATTACCT
CGGACGTCTACGGAAACGAGCGGGCGCATTGTTGTCCGCTTGTGTGTGTGTGTGTGTGTG
TGTGTGTGCGCGTGCATTGATTACTATCCATTTCTTTAGTCAACGCTCTCCACTTCCTGA
TTTCTGCTTTAAGGAAAACTGTGAACTTTCTGCTTCATGTATCAGTTTTAAAGCAGCCCA
GGCAAAGATCATCTACAGATTCTAGGAATTCTCTCCCCTGAAATCAAAACCTGGAAGACT
TTTTTTTCTTATTTTAGTTGAGAAGTTTCATAAACTGCTCAAGGATTAGTTTTCCAGGAC
TCTGCGGAGGAACGGCAGGAAGAACCTCAGAGAGGGCAGAGGTGACTTCAAAGTGCTGGG
GACTCCGTCCTGAGGGTCACTTGGCCCTGAGCCCCTGCGTGCCCTTGCGGAAGCCCAGAA
GCTTCTTCCTGCTGCACCTCCCGTTTCCGCTGCTGCTGACGTTTATGCATTTCATGATGG
GGTCCAACAAGAACACCTGACTTGGGTGAAGTTGTGCAATATTGGAGGCTGACTGTAGGG
CTGGGCAGCTGGGAGACAGGCTCATGGCTCATGGCTCATGGCTCAGGGCGGTGCCTGCCA
TGGGCCGGGACCCCCCTCCCCACCCCCCACCTAGGCTTTTTGGGTTTTGTTCAAGGAAGG
TAAAGTGAGAGGTTTAGGTCAGTGTTTTTAAGTTTTTGTTTTTTTTTTAAAGCAAATCCT
GTATATGTATCTACATGGGAGACAGGTAGACACTACTTATTTGTTACATTTTGTACTACA
CGTTTGTGTTCCAGGTTTCAGCTTCCCTCGCTCCTGTTGTTAAGAAGCGTCCCTGTCAGC
ACAGGTGTGCATTGAGGAAGGGGCCCCAGGGCCTTCGCTCCCTCAGCACTGGGGTGGAGG
CGGCAGGAAGGGGCGGCCCTTACCTGGCAGGTCTGGGCGCACCTTTAGCAGGTGGACTCC
GTGGGGCTCCACCAGCCAGAAGCCTTTGGAAGGCAACGAAGGCAATGCTGCTCCCTGAGT
CCAGTCCCCGCCCCCAAACCCAGCCCAGGTGCCTTCAGCTACTTCGGCTTCTTAAACCCT
GCAGTGTTAAACAGAGGCATTGAGAAAGGGGAAAGGCGGGTATTTTTAAAAGCCAAAGAT
TGACCCAGTTACTTGAGGGTAGGGAGGCGGGCCCAGTGCAGGAGGCTGCATCCCTGGCCT
GCTGGTGCCCACCGGGGGCTGTGCCTGTGCCGGGCCGCAGGGAAGCTGGCTGCCCCCATT
CCTGCTGCTGCTGCTGCTGCTGCTCTGTGGCTGTTTCAAAGACTGGGCGAAAGGCTGTCC
GGAGGGCAGACCAGGTGCCTTGCCGCAGAGAAAACACCAAAGTCTCCTGTTCGCTCATAA
AGAAGTTTTTGGGATGGGAGAGAATCCAGACCATCTTGGGGCAGCCAGGCCCTTGCCTTC
ATTTTTACAGAGGTAGCACAATTGATTCCAACACAAAACTTCCCCTTTTTAAAATGATTT
CTGTTCTAATGCCATAGATCAAAGGCCTCAGAAACCATTGTGTGTTTCCTCTTTGAAGCA
ATGACAAGCACTTTACTTTCACGGTGGTTTTTGTTTTTTCTTATTGCTGTGGAACCTCTT
TTGGAGGACGTTAAAGGCGTGTTTTACTTGTTTTTTTAAGAGTGTGTGATGTGTGTTTTG
TAGATTTCTTGACAGTGCTGTAATACAGACGGCAATGCAATAGCCTATTTAAAGACACTA
CGTGATCTGATTGAGATGTACATAGTTTTTTTTTTTACCATAACTGAATTATTTTATCTC
TTATGTTAACATGAGAAATGTATGCCAAATGATTAGTTGATGTATGTTTTTTAATTTAAT
ATTTAAATAAAATATTTGGGAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MVIMSEFSADPAGQGQGQQKPLRVGFYDIERTLGKGNFAVVKLARHRVTKTQVAIKIIDK TRLDSSNLEKIYREVQLMKLLNHPHIIKLYQVMETKDMLYIVTEFAKNGEMFDYLTSNGH LSENEARKKFWQILSAVEYCHDHHIVHRDLKTENLLLDGNMDIKLADFGFGNFYKSGEPL STWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGPNLPTLRQRVLEGR FRIPFFMSQDCESLIRRMLVVDPARRITIAQIRQHRWMRAEPCLPGPACPAFSAHSYTSN LGDYDEQALGIMQTLGVDRQRTVESLQNSSYNHFAAIYYLLLERLKEYRNAQCARPGPAR QPRPRSSDLSGLEVPQEGLSTDPFRPALLCPQPQTLVQSVLQAEMDCELQSSLQWPLFFP VDASCSGVFRPRPVSPSSLLDTAISEEARQGPGLEEEQDTQESLPSSTGRRHTLAEVSTR LSPLTAPCIVVSPSTTASPAEGTSSDSCLTFSASKSPAGLSGTPATQGLLGACSPVRLAS PFLGSQSATPVLQAQGGLGGAVLLPVSFQEGRRASDTSLTQGLKAFRQQLRKTTRTKGFL GLNKIKGLARQVCQVPASRASRGGLSPFHAPAQSPGLHGGAAGSREGWSLLEEVLEQQRL LQLQHHPAAAPGCSQAPQPAPAPFVIAPCDGPGAAPLPSTLLTSGLPLLPPPLLQTGASP VASAAQLLDTHLHIGTGPTALPAVPPPRLARLAPGCEPLGLLQGDCEMEDLMPCSLGTFV LVQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
