Gene:
Gene Symbol:
MAP4K5
Gene Name:
mitogen-activated protein kinase kinase kinase kinase 5
Original Clone ID:
HsCD00298663
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618309
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2541nts
Open reading frame: 259 to 2799
GGGGGGACGCGCCGTGCAGCGGCTGAGTGGCGGCGGCGGCGACGGTAAACCCGGAGCTGC
CGGCCGGCGCGCGGGAGGAGGACGCGGGTGCGGTCTAGGAAGTAGGTCCCGGGCTCCGCG
CTCAACAAAGACGGGGCCGCCCCCATTAGACGGAGCTGCGGGCGGAGGCTCCATGTTGGG
AAGCGGCGCCGTTCGTGCTTGTTAGCGGGAATCCGGGAGCCGCGGGGTGAGCTGGCGGGG
GCCGGGCCCTAAGTGAAGATGGAGGCCCCGCTGCGGCCTGCCGCGGACATCCTGAGGCGG
AACCCGCAGCAGGACTACGAACTCGTCCAGAGGGTCGGCAGCGGCACCTACGGGGACGTC
TATAAGGCCAGAAATGTACACACAGGAGAGCTGGCTGCAGTAAAAATCATTAAATTGGAG
CCTGGAGATGATTTTTCTTTGATTCAACAAGAAATATTTATGGTTAAAGAATGTAAACAT
TGTAACATCGTTGCCTACTTTGGGAGTTATCTTAGTCGGGAAAAACTATGGATTTGTATG
GAATACTGTGGTGGCGGATCACTTCAAGATATTTACCATGTTACTGGACCATTATCAGAA
TTGCAAATAGCCTATGTATGCAGAGAAACCTTACAGGGTCTTGCCTATTTGCATACTAAA
GGCAAAATGCATAGAGATATCAAAGGTGCTAATATTTTATTGACAGACCATGGCGATGTA
AAATTAGCTGACTTTGGTGTGGCTGCAAAAATAACAGCTACCATTGCAAAACGAAAATCT
TTCATTGGCACCCCTTACTGGATGGCCCCAGAAGTTGCAGCAGTAGAGAAGAATGGTGGC
TACAACCAACTCTGTGATATCTGGGCAGTAGGAATAACAGCAATTGAACTTGGAGAACTT
CAGCCACCTATGTTTGATCTCCACCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTAAT
TTTCAGCCTCCAAAACTAAAGGACAAAACAAAATGGTCATCAACATTCCATAATTTTGTC
AAAATAGCACTAACCAAAAACCCAAAAAAAAGACCAACTGCTGAAAGACTTCTGACTCAC
ACTTTTGTTGCACAGCCAGGTCTCTCTAGAGCCCTAGCAGTTGAACTGTTAGACAAAGTG
AACAATCCAGATAACCACGCACATTACACTGAAGCAGATGACGATGACTTTGAGCCCCAT
GCAATCATTCGTCATACCATTAGATCTACAAACAGGAATGCCAGAGCTGAACGGACAGCT
TCAGAAATAAATTTTGACAAATTACAATTTGAACCTCCTCTGAGAAAAGAAACAGAAGCA
CGAGATGAAATGGGATTGTCATCAGACCCAAATTTCATGTTACAGTGGAATCCTTTTGTT
GATGGTGCAAATACTGGCAAATCAACCTCAAAACGTGCAATACCACCTCCCCTACCTCCT
AAGCCAAGGATAAGCAGTTACCCTGAAGACAACTTTCCGGATGAAGAAAAAGCATCAACC
ATAAAACATTGTCCTGATTCAGAAAGCAGAGCTCCCCAAATTCTCAGAAGACAGAGTAGC
CCAAGTTGTGGGCCTGTGGCAGAGACTTCTTCTATTGGAAATGGTGATGGTATTTCAAAA
CTGATGAGTGAAAATACAGAAGGATCAGCACAAGCACCACAGTTACCACGAAAAAAGGAC
AAACGAGACTTCCCTAAACCAGCCATCAATGGCCTTCCACCCACCCCAAAAGTTCTGATG
GGAGCATGCTTTTCAAAAGTTTTTGATGGCTGTCCTTTGAAAATTAATTGTGCAACATCC
TGGATACATCCTGATACAAAAGATCAGTACATTATTTTTGGAACTGAAGATGGTATTTAC
ACACTGAATCTCAATGAGCTACATGAGGCAACGATGGAACAGTTATTTCCACGGAAGTGT
ACTTGGCTGTATGTTATCAATAATACTTTAATGTCATTATCAGAAGGAAAAACCTTTCAG
CTCTACTCTCACAATCTTATAGCTTTGTTTGAACATGCCAAAAAACCAGGATTAGCTGCC
CATATTCAAACTCACAGGTTTCCAGACCGAATACTACCAAGAAAATTCGCTTTAACAACA
AAGATTCCTGATACAAAAGGCTGCCACAAATGTTGCATAGTCAGAAACCCTTACACGGGA
CATAAATACCTCTGTGGAGCTTTACAGTCTGGAATTGTTTTACTTCAGTGGTATGAGCCA
ATGCAGAAATTCATGTTGATAAAGCACTTTGATTTTCCTTTGCCAAGTCCTTTGAATGTT
TTTGAAATGCTGGTGATACCTGAACAGGAATACCCTATGGTCTGTGTAGCTATTAGCAAA
GGCACTGAATCGAATCAGGTAGTTCAGTTTGAGACAATCAATTTGAACTCTGCATCTTCA
TGGTTTACAGAAATTGGTGCAGGCAGCCAGCAGTTAGATTCCATTCATGTAACACAGTTG
GAGAGAGATACCGTTTTAGTGTGTTTAGACAAATTTGTGAAAATTGTAAATCTACAAGGA
AAATTAAAATCAAGTAAGAAACTGGCCTCTGAGTTAAGTTTTGATTTTCGCATTGAATCT
GTAGTATGCCTTCAAGACAGTGTGTTGGCTTTCTGGAAACATGGGATGCAGGGTAAAAGC
TTCAAGTCAGATGAGGTTACCCAGGAGATTTCAGATGAAACAAGAGTTTTCCGCTTATTA
GGATCAGACAGGGTTGTCGTTTTGGAAAGTAGGCCAACAGAAAATCCTACTGCACACAGC
AATCTCTACATCTTGGCTGGACATGAAAATAGTTACTAAGCAACAGAAACTGATCTCAAA
TGACAGGAAAATGAATATACTCCATTGAAAGGAAAAATAAGGAAATTCAATACAAACTGC
ACTATGATTTGCTTTAACTATTATGGGTTATATTGCAAATGATCTGTACTTTAGGGTAGA
ATTCAATATTTTCTGCAGCTGGAAACAGCTAGTCTATCTCTTGCCACTGTGTGGTGGTTA
TATCAAGTTTGCTTAATAAAAGCTATGAGACAAATAGTCCTCTAGTTCCAGGAAACACAG
TCTTTTTTTAAAAAAAACAATGTTTGTAACAAGGGTGCCATGGTATTTTTAGATAACTCG
TGATTATCTTAAGAGAGGTAAATTTAGTGATCATTTTATATCATGTCTTATTCCTTCTTA
ATGAACATAATTTGTTAAATTCTCAAGCAAGGTTTTCACTTTTATATTGGCCATTCTGTA
TGTTTTTGTAAAACAGAATATTTAATCCTTATTTATTAATCTCTTGCTGGAGTGGTGTAA
TGTATCTAACTTTTAGCAAAGGAGGGTTGCAGAGCAGCTTAAATTTTTTTTATAATGTAT
AAGAATTTTGTTTATCTTTTAAGAGTAGTAAAGTACTTTGAGTGTTTGGGGGTTCAACAC
ACACATGCAATTTTGCTTAACAAAAGTATTTTATAATACAGTTTCATACAGAATTACCTT
AAAAGGGAGTCTTATGTTTTCAACTACAGATAGTTGTAAGGGATCATACAGAAGATATTG
ATGATAGTTGAAATATTCTTAGAAGGGGTGTGTATGTCTAGCTGTGTCTACCATGTGTAT
GTATTCTTGACAAGCAGTATAAAATACCTGTGATTTTTCTTTACATTAGGGATAATGCAT
AAGGAATTAATCTTCATATATATTATCATCCCTAATGTAGCAGGGGGAAGTATTTAATTG
CCCATGATATGTATTTTACTTATACTATGCCAGAGAGGAAACTATAAAGTAATTACACAT
GTAATCTTGGGTTTTTCACATATGTAGGTATTCATTTTGAGTAGGTTGAAGAAGAAAAAA
AATATTTAAATGAATTGAATTCCTGATGGGATAGTATCAATAAGTATTTAAAAGCCAGTA
TTCTAAAAATAATAAAGGGTAGGGTCATTTTTGAGTTTGTTTTTCTTTTGCTATTGTTAA
TATTCAAAATTAAAGTGTTACATTGGTACCTGTTGTCTTAATGCATTTATTGAGAACAGC
ATTGAGATGATGAACAAGGGGTTAGCAATAGCAAACTCTATAATTATTTTGACTAATTAC
TTAAGAGGAAAACAGTATAAGTATCTCATTCAGTATTTAGCAATTCTGTAAAATAAGTAT
TATCTCTATTTTTCAGATGAGGAAGTAAGGGTTTAGCAAGGTTAAGAGATCTATCCAATT
TACACAGCAAGTTAGTAGTTGAGCCTGACCATGAGTCTTCTGACTCTGTTCTTTTCACTA
TGCAATACGCAAACAATAAAATGTTATACAAATAGAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MEAPLRPAADILRRNPQQDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFS LIQQEIFMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSELQIAYV CRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPY WMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPMFDLHPMRALFLMSKSNFQPPKL KDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTFVAQPGLSRALAVELLDKVNNPDNH AHYTEADDDDFEPHAIIRHTIRSTNRNARAERTASEINFDKLQFEPPLRKETEARDEMGL SSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPD SESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLPRKKDKRDFPK PAINGLPPTPKVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEDGIYTLNLNE LHEATMEQLFPRKCTWLYVINNTLMSLSEGKTFQLYSHNLIALFEHAKKPGLAAHIQTHR FPDRILPRKFALTTKIPDTKGCHKCCIVRNPYTGHKYLCGALQSGIVLLQWYEPMQKFML IKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGTESNQVVQFETINLNSASSWFTEIG AGSQQLDSIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFDFRIESVVCLQD SVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPTAHSNLYILA GHENSY*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
