Gene:
Gene Symbol:
ADCK3
Gene Name:
aarF domain containing kinase 3
Original Clone ID:
HsCD00299193
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618314
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1944nts
Open reading frame: 122 to 2065
GTTGGTAAACAGATCCGGAGCGCGTGGCGGGCGTCAGCGCGGTGGCCAGCGCGCAGAGGC
GGGCGCGGAGGCGGCTAGAAGGTGACCGCGGATCCCAGCTTCCTGCAGCCAGCCCTGAAG
GATGGCTGCCATATTGGGAGACACCATCATGGTGGCTAAAGGCCTTGTCAAGCTGACCCA
GGCGGCCGTGGAAACCCACCTGCAGCACTTGGGCATCGGAGGGGAGCTGATCATGGCGGC
CAGGGCCCTGCAGTCCACGGCTGTGGAGCAGATTGGCATGTTCTTGGGGAAGGTGCAGGG
TCAGGATAAACATGAAGAATATTTTGCTGAGAACTTCGGCGGCCCAGAAGGGGAGTTCCA
CTTCTCAGTCCCGCATGCAGCCGGAGCCTCCACAGACTTCTCTTCAGCCTCCGCTCCCGA
CCAGTCAGCGCCCCCATCCCTGGGTCATGCCCACAGCGAGGGCCCAGCTCCTGCCTACGT
GGCCAGTGGACCCTTTAGAGAAGCCGGGTTCCCCGGCCAGGCCTCCTCCCCTCTGGGCAG
GGCCAACGGGAGGCTCTTTGCAAACCCCAGAGACTCATTCTCTGCCATGGGCTTTCAGCG
AAGGTTCTTCCACCAGGACCAATCCCCTGTTGGGGGCCTCACAGCCGAGGACATTGAGAA
GGCCCGGCAGGCTAAGGCTCGCCCCGAGAACAAGCAGCACAAACAGACGCTCAGCGAGCA
TGCCCGGGAGCGGAAGGTGCCTGTGACGAGGATTGGCCGGCTGGCCAACTTCGGAGGTCT
GGCCGTGGGCCTGGGCTTCGGGGCACTGGCAGAGGTCGCCAAGAAGAGCCTGCGCTCCGA
GGACCCCTCAGGGAAGAAGGCCGTGCTGGGTTCCAGTCCTTTCCTGTCCGAGGCCAATGC
AGAGCGGATCGTGCGCACGCTCTGCAAGGTGCGTGGTGCGGCACTCAAGCTGGGCCAGAT
GCTGAGCATCCAGGATGATGCCTTTATCAACCCCCACCTGGCTAAGATCTTCGAGCGGGT
GCGGCAGAGCGCGGACTTCATGCCACTGAAGCAGATGATGAAAACTCTCAACAACGACCT
GGGCCCCAACTGGCGGGACAAGTTGGAATACTTCGAGGAGCGGCCCTTCGCCGCCGCATC
CATTGGGCAGGTGCACTTGGCCCGAATGAAGGGCGGCCGCGAGGTGGCCATGAAGATCCA
GTACCCTGGCGTGGCCCAGAGCATCAACAGTGATGTCAACAACCTCATGGCCGTGTTGAA
CATGAGCAACATGCTTCCAGAAGGCCTGTTCCCCGAGCACCTGATTGACGTGCTGAGGCG
GGAGCTGGCCCTGGAGTGTGACTACCAGCGAGAGGCCGCCTGTGCCCGCAAGTTCAGGGA
CCTGCTGAAGGGCCACCCCTTCTTCTATGTGCCTGAGATTGTGGATGAGCTCTGCAGCCC
ACATGTGCTGACCACAGAGCTGGTGTCTGGCTTCCCCCTGGACCAGGCCGAAGGGCTCAG
CCAGGAGATTCGGAACGAGATCTGCTACAACATCCTGGTTCTGTGCCTGAGGGAGCTGTT
CGAGTTCCACTTCATGCAAACAGACCCCAACTGGTCCAACTTCTTCTATGACCCCCAGCA
GCACAAGGTGGCTCTTTTGGATTTTGGGGCAACGCGGGAATATGACAGATCCTTCACCGA
CCTCTACATTCAGATCATCAGGGCTGCTGCCGACAGGGACAGGGAGACTGTGCGGGCGAA
ATCCATAGAGATGAAGTTCCTCACCGGCTACGAGGTCAAGGTCATGGAAGACGCCCACTT
GGATGCCATCCTCATCCTGGGGGAGGCCTTCGCCTCTGATGAGCCTTTTGATTTTGGCAC
TCAGAGCACCACCGAGAAGATCCACAACCTGATTCCCGTCATGCTGAGGCACCGTCTCGT
CCCCCCACCCGAGGAAACCTACTCCCTGCACAGGAAGATGGGGGGCTCCTTCCTCATCTG
CTCCAAGCTGAAGGCCCGCTTCCCCTGCAAGGCCATGTTCGAGGAGGCCTACAGCAACTA
CTGCAAGAGGCAGGCCCAGCAGTAGGGCTGCGGGCCACGCCCAGGCCGGCTCCGCGGGAA
CTCTCTCCCTCAGACAGGCCAAAAACCAGTAGCGAGGTCGTGGTGATGCTCTTTTTAACT
CCTTTGCCCAATAAGGGGGGTGGCTGCCTGGAGCCCCGTAGCCAGCGCTTTCCACGGTTT
CTGTTGCTAAATGGTTGTAGGGTGAGAAGTGCAAGAATGAAGATGAAGCCCCACTGCTCG
GTCAGTCTGCCTCCGTGTGTCCTCTGAAATAAGCAGATGAAGATGAAAGGGCAACTTTGT
TTTCTTCTTTTTCCTGATGTGAATGTTAAGCAGAAGGGAGAGAGTCCTTACTCCCTTCCA
ATCTCTGTTCAGTGCAAAACCCAGAAACATGAACAGATACGATTGTGGGATTTTATCATC
TGTGTAGTAGGTGTGTGTATGTGTTTCTAGAGTGAGATTTGTGTTTTCTGCCCTTTTCCT
CTCCAGCCAATGGGCTGGAGCTGGGAGAGGTGCTGAGCTAACAGTGCCAACAAGTGCTCC
TTAAGCCTGCGAGGCCCAGGCCTGTGGGGCTGGTTCTCACCTTTGACAGCTGAATGTTCC
TAAAGAACTGCTGCCCCACAGTGAGGGTGGGAGCAGCGGAACAGGGAATGCCAGACACAG
GCTCGCTGCTGCTGGAAGGCGGGGTGGGACTTCCTTCCTCTGTCCGGAGAGGCACAGGTG
TCACCAGTTCCAGCCAAAGGCTCCTCACAGGCGCTGTGAATTTTTGTACAAGTCTTGTAA
TTATCGAATCAACAACTTGTTTCAATTTAATAAAAATGCTCATGGGAAGTGAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MAAILGDTIMVAKGLVKLTQAAVETHLQHLGIGGELIMAARALQSTAVEQIGMFLGKVQG QDKHEEYFAENFGGPEGEFHFSVPHAAGASTDFSSASAPDQSAPPSLGHAHSEGPAPAYV ASGPFREAGFPGQASSPLGRANGRLFANPRDSFSAMGFQRRFFHQDQSPVGGLTAEDIEK ARQAKARPENKQHKQTLSEHARERKVPVTRIGRLANFGGLAVGLGFGALAEVAKKSLRSE DPSGKKAVLGSSPFLSEANAERIVRTLCKVRGAALKLGQMLSIQDDAFINPHLAKIFERV RQSADFMPLKQMMKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARMKGGREVAMKIQ YPGVAQSINSDVNNLMAVLNMSNMLPEGLFPEHLIDVLRRELALECDYQREAACARKFRD LLKGHPFFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEIRNEICYNILVLCLRELF EFHFMQTDPNWSNFFYDPQQHKVALLDFGATREYDRSFTDLYIQIIRAAADRDRETVRAK SIEMKFLTGYEVKVMEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPVMLRHRLV PPPEETYSLHRKMGGSFLICSKLKARFPCKAMFEEAYSNYCKRQAQQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
