Gene:
Gene Symbol:
TPPP
Gene Name:
tubulin polymerization promoting protein
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618357
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 660nts
Open reading frame: 34 to 693
GCCTCCGCTGCAGTCGCTGCGTCTCCAAGCAACATGGCTGACAAGGCCAAGCCTGCCAAA
GCTGCCAACAGGACGCCCCCCAAGTCCCCGGGGGACCCCTCGAAGGACCGGGCAGCCAAG
AGGCTGTCGCTGGAATCGGAGGGTGCTGGTGAGGGGGCAGCCGCATCCCCTGAGCTCAGT
GCCCTGGAGGAGGCCTTCCGGCGCTTTGCCGTGCACGGGGACGCCAGGGCCACCGGGAGG
GAGATGCACGGCAAGAACTGGTCGAAGCTGTGCAAGGACTGCCAGGTGATCGACGGCAGG
AACGTGACCGTCACTGACGTGGACATCGTCTTCAGCAAGATCAAAGGGAAGTCTTGCCGG
ACCATCACCTTTGAGCAGTTCCAGGAGGCGCTGGAGGAGCTCGCCAAGAAGCGATTCAAA
GACAAGAGCAGCGAGGAGGCCGTTCGCGAGGTGCACAGGCTCATCGAGGGCAAGGCGCCC
ATCATCTCAGGGGTGACGAAAGCCATCTCGTCGCCCACAGTGTCGAGGCTCACGGACACC
ACCAAGTTCACGGGCTCCCACAAGGAGCGCTTCGACCCCTCTGGCAAGGGCAAGGGCAAG
GCTGGCCGCGTGGATCTGGTGGACGAGTCAGGCTATGTGTCCGGCTACAAGCACGCAGGC
ACCTACGACCAGAAGGTGCAAGGGGGCAAGTAGCCCCCGCTCCATGCCTCGCGGCACTGC
CGGTGTCCCCAGAGCAGGGACTCTGTCACCTCGCACTTCATTACATTCCTGTACTAACTG
GGGCAGAACTCAGACGGGTGCCCCAGAGGGGGCTGGGGGGCGGCCAGGCCCAGGCCTCCC
TCCTGCCCCTCCTCCTACCGGATGCCCCCAGCACTCCCCTCTCAAACCAGGTTTGGGCCC
CAGTTCGCTGACCCTCCTAATACACCTGCCTCGTCCTCAGCCATTTCCAAAGTGTCTCGC
GGATCACACCACACTGGGCACGTGGTTTGCAGGTCAAAGGGGCGTTTTAAAGCAGCTGGC
TGTCATGGCAACAGGAGGCTGTGCTGACCTCCTGAGCGGCAGACACCTTCCAGGAGCCCT
GAGGGTGGCAGGAGCTAACCCCAACCAGCAGGCAACTAACACGGAATTGGCCCCACACCG
GACGTGGGAGGTGTCTGTGGGGCCCGAGGCCTGTCCTGTGTGCAGCGGACACCACGGGGC
CTTCCTGCTTTCCTGGGCAGAGGGCAGAGTGAGGCCACCTGGCGGGGGTGCTGGGCGCCT
GGCACATGTGTGGGGAAGCCGGTCACATGGACACACCTGTGCACACATGCCTACAGGCCA
GCTCTGTGCCAAGGGCAACCTAGGTAAAACGAAAGCCGTCAGGGGCAGTGGGCGGCTTCC
CGGCTGACCACAGTGGCTTGGACTGTGAGGGTAGAGTAGGCTCGCTTTGCTTTCCTGAGA
AGATGCGGTGGCTGCCTATGTTCTCAGAGCGGGTCTGGGAAGATTCAGAATGTCCGGTCC
CTGTGGTGTTGCCAGGCAAGAGACACGAAGTGCCGAGACACTCCTCGCCTCACCGCGTGA
CAGAGCCTCTGCCCGGCCCTCCCGTTCGCCCGTCCTCACTAGCTGCACCCTGTTTGCTCG
CAGACCTCCCATTGCCACAGCCCAAGCACCTTCTTCAACTCTCCCAAAATGGCTCAGCCT
AACCTCTCCTGGCCAATCCCCCCCGCCGGAGAGCAGGACACTAGGGAGGACCCCCAGTCC
TGCAGTGTCTGTGGGGGTTTCTCTGCCAGCAGGGGGCTGAGCAGAGCCCATCCAGGACAC
TCCACACTGCCAGGACACACCCAGGCGGCCCGCCCTTGCCTGTCTGCACCTGGGGAGAAG
CCGGCGCTCCTGCTCCCTCCTGGGGAGGCTGACGGGTGTGGCCACCCGCTGTCACAATGG
CACACTGCCACTGTCCCTTGGCACGCACACAGCCACAGCCACACGTGTGACCTGCTGGGC
CGTGGTTCTGGAGTCTACCTGCGGATGAGCCTGCGGCAGTCCTGGGGAAACTTTTCCAGA
GCCTGTTAGCCCGTGGCTACGGTCAGGCTCTGGCCAGGGCAGAGGGCTGCCCAGGGCCAG
GCTCACAGTACAGGAGGGTGGCGAGGCCCCTCCCTCACTGGCACGCATGAGCACCACCCG
CCTCCCCGACTCCCAAGAGTGCACCTGCTGCGGCCACAGCTCCGTGGAAGGACTCCCCCT
ACCTGAGCAGAGCAGAAGCCCCAGGGCGGAGCTCCCAGCCAGCATGGTCCGCTGAGGGCT
GGGGGGCGGTCTCCGAGGCCCCTCAACAGAGAAGCCTCCACCTGAGGATGGGGAGGACCT
GGCAGGCAGCTTCCACGGCAGGGCTGGGAAGTTCAGTGCCTGGAAATAAAGAGCAAGGAA
AAATGGACCTCAGGCTTCGTGGCTCCTTTAGGATGTCACCTCACCGGCCTGGGGAAGGCG
GGGGGTGCCCAAGCCCAGCCCTGTGCCCCCGCTGAACCTGGCTGGACCGCGTGTGAAAGG
CAGAACTAACGTGCGGAAACATTTGAAAACAACTCTGAATGTGCGGTTCGGAATCACCCG
ATCACAGACGAGCGGTCACCGGAATCGCCCGGTCACAGACGAGCAGTCATCGGACTCACC
CGATCACAGACGAGCGGTCACCGGAATCGCCCGATCACAGACGAGCGGCCACCGGAATCG
CCCAATCACAGACAAGCAGTCATCGGAATCACCCGATCACAGACGAGCAGTCATCGGACT
CACCCGATCACAGACGAGCGGTCACCAGACTCGCCCGATCACAGACGAGCAGTCATCGGA
ATCACCCGATCACAGACGAGCAGTCATCGGACTCACCCGATCACAGACGAGCGGTCACCA
GAATCGCCCGATCACAGACGAGCAGTCATCGGAATCACCCGATCACAGACGAGTGGCCAC
CGGAATCACCCGATAACAGCCCATCACAACTCCAAAGCCCTTGTGTTGAAAAGGCCGAGG
ACGAGTGGTCACCAAACAGGGTGGCTCCCCAGGTCCCGAAGCCTGAGACCCGGAAGGCCC
TGGCACCCTTACACCCTCGGACTCCTGCCCTCCTCGGCCTCCCTGGCCCCAGCGCGGCTC
CACCCTGGGCTGCGTCTCCTGGTCACAGGCTGCGTTTCTCCTTTCTGTCCGTGGGCAGCC
CAGTCCCCACAGTCACGGCCAAGCCACGCAGAACGAACATGACTCCAGAGGACCTCGCCC
TGGAGCTGAGCCTGGCGCCGGGTCAGGACGGAGGGAAGGGCGGGTTGGGGTCCGCGGGTC
CTCACACCGCACGGCAGGCACAGAGGCTCGCCAGGCCCTGATCCGGTCTGTGGGGACGAG
GGACACTGAGGAGAGGTGCTGGGCACCAGGACGCTGCCTCCTGGTCCCTGGTTGGCCTCA
GACAAGCACGGCCTCGAGAAAAGAGCCAAGCGCATCGGGAGCACACCAGAAACCGGCCCT
GAGCACGAGAAGAGCCTCCGCCCGGCCCGGGCACCACCCGACCTCGCAGGGAGCAGGCCC
TGTCACCGACGGGTCACCTCGCCCACACGGCCAGCACCGACTCACCACAGCCCTTCCCAG
ACCTGGCTGGAGTGGCCGGAGCGGCGGGGCTCATGGCTCCCATCTTGGCCCCTGGAGGTG
AGCTCATTCACAGAAGTGGTCCCTTCACTCTGAGAGAGAAAATCGTGGCGTGCATCCCAA
ACCCTAGGCCACGCCTGTGGGTTTCGTGAATGAGATCGAGGCTGCTGTGGCACCCTGCCC
GTCCTGGCCTGGAGCACCCTGGGATCCTGGAGGGAGAGGCCCCACGCCCCCACTCTCCCT
CCACACTTCCAGGGTTGGTGCCCACGAGTTAGAGGCACGGCCTCCCCCAGCGGCCCTCAG
GCTTTTCTCAGACATGCGGGAGCCAGGAGAGCACCCTTCTCCACTCAGCTCCAAAGCGAA
TCTTTGAAAACACCCACTCGGCTCCCATCTCTGTCACCTAGCCAGGAGGGGTAGCAAAAA
TAAAGTCACGAGGACATAGTGGTCACCACTGTCAGTTACAACTTGTCTGTGGAATCCGTA
ATTGCATCTGTGTGCCGCCTCCGAACACAGAACATTGTTTGGACGGCAGCCCCACTGCAC
ATAACAGACCCGTGCATCTTCCTCAGTCAGTTTCTGAATATTGTGAATTCAGGCAGGTGT
GTGTTCTCTTCTGCATGTTTTTATGCACTGCCAATTAGCTTCTACTAACCACGGTTCAAC
AGAAAATAAATGTGTATTTGTGAATAACAAACTGCACAACCTGCAAACCAGGAGAGAGGG
ACAGGTTCTGTCAGGGGTGACACCAGGACACAGGGACAGGTTCTGTCGGGGGTGACACCA
GGACACAGGGACAGGTTCTGTCATGGGTGACACGAGGACACAGGGACAGGTTCTATCATG
GGTGACACCAGGAGAGAGGGACAGGTTCTGTTGGGGGTGACAAGAGGAGACAGGGACAGG
TTCTGTCGGGGGTGACACCAGGAGAGAGGGACAGGTTCTGTCAGGGGTGACACCAGGAGA
GAGGGACAGGTTCTGTTGGGGGTGACACCAGGACACAGGAATAGGTTCTGTCGGGAGGAC
AGTGCTGATCGTGTCTCAGCATCAGGAAAGGAGAAAGGCAGAGGGAGAGCGCTGAGAAGA
CTGTTCACGCCAGAGTGCTTATTTATTTTTAATTTACTGCTATAGGATAAGCAACCAGGT
AGTGTTCCTAACAATTAGCGTTACCAAAATTAAAGTTCAAATTATATGTTTAAAATATTG
TAGAAGATATATATTTATACTGGACTACTTTTACACCTTCTAATATCCTGTCCAAGTTTG
GGCGCAGATGGTGGAGTTGGGCTGGCATCATGTCCTGTGGCCGCCCCACTTGCCTGTTGG
TGCCACTCCATCCCGGGCCCCAGGGATGCCAGCTCAGGGCTGACCACAGCAGCCCTGCGT
GGGCATCACCTCCTACCCCAGCCCCCATCCTGGGCTGCT
Protein sequence predicted based on nucleotide sequence
MADKAKPAKAANRTPPKSPGDPSKDRAAKRLSLESEGAGEGAAASPELSALEEAFRRFAV HGDARATGREMHGKNWSKLCKDCQVIDGRNVTVTDVDIVFSKIKGKSCRTITFEQFQEAL EELAKKRFKDKSSEEAVREVHRLIEGKAPIISGVTKAISSPTVSRLTDTTKFTGSHKERF DPSGKGKGKAGRVDLVDESGYVSGYKHAGTYDQKVQGGK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
