Gene:
Gene Symbol:
DLGAP5
Gene Name:
discs, large (Drosophila) homolog-associated protein 5
Original Clone ID:
FLH272797.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618359
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2298nts
Open reading frame: 353 to 2650
GGAGGCTCGGGTTGTGAGGGTTCCTGCTTCGGAGTCGGCGGTGGTCGTCCAGACCGAGTG
TTCTTTACTTTTTGTTTGGTTGAGGTTTCACGCTAGAAGGTGGCTCAGGATGTCTTCATC
ACATTTTGCCAGTCGACACAGGAAGGATATAAGTACTGAAATGATTAGAACTAAAATTGC
TCATAGGAAATCACTGTCTCAGAAAGAAAATAGACATAAGGAATACGAACGAAATAGACA
CTTTGGTTTGAAAGATGTAAACATTCCAACCTTGGAAGGTAGAATTCTTGTTGAATTAGA
TGAGACATCTCAAGGGCTTGTTCCAGAAAAGACCAATGTTAAGCCAAGGGCAATGAAAAC
TATTCTAGGTGATCAACGAAAACAGATGCTCCAAAAATACAAAGAAGAAAAGCAACTTCA
AAAATTGAAAGAGCAGAGAGAGAAAGCTAAACGAGGAATATTTAAAGTGGGTCGTTATAG
ACCTGATATGCCTTGTTTTCTTTTATCAAACCAGAATGCTGTGAAAGCTGAGCCAAAAAA
GGCTATTCCATCTTCTGTACGGATTACAAGGTCAAAGGCCAAAGACCAAATGGAGCAGAC
TAAGATTGATAACGAGAGTGATGTTCGAGCAATCCGACCTGGTCCAAGACAAACTTCTGA
AAAGAAAGTGTCAGACAAAGAGAAAAAAGTTGTGCAGCCTGTAATGCCCACGTCGTTGAG
AATGACTCGATCAGCTACTCAAGCAGCAAAGCAGGTTCCCAGAACAGTCTCATCTACCAC
AGCAAGAAAGCCAGTCACAAGAGCTGCTAATGAAAACGAACCAGAAGGAAAGGTGCCAAG
TAAAGGAAGACCTGCCAAAAATGTAGAAACAAAACCCGACAAGGGTATTTCTTGTAAAGT
CGATAGTGAAGAAAATACTTTGAATTCACAAACTAATGCAACAAGTGGAATGAATCCAGA
TGGAGTCTTATCAAAAATGGAAAACTTACCTGAGATAAATACTGCAAAAATAAAAGGGAA
GAATTCCTTTGCACCTAAGGATTTTATGTTTCAGCCACTGGATGGTCTGAAGACCTATCA
AGTAACACCTATGACTCCCAGAAGTGCCAATGCTTTTTTGACACCCAGTTACACCTGGAC
TCCTTTAAAAACAGAAGTTGATGAGTCTCAAGCAACAAAAGAAATTTTGGCACAAAAATG
TAAAACTTACTCTACCAAGACAATACAGCAAGATTCAAATAAATTGCCATGTCCTTTGGG
TCCTCTAACTGTTTGGCATGAAGAACATGTTTTAAATAAAAATGAAGCTACTACTAAAAA
TTTAAATGGCCTTCCAATAAAAGAAGTCCCATCACTTGAAAGAAATGAAGGTCGAATTGC
TCAGCCCCACCATGGTGTGCCATATTTCAGAAATATCCTCCAGTCAGAAACTGAGAAATT
AACTTCACATTGCTTCGAGTGGGACAGGAAACTTGAATTGGACATTCCAGATGATGCTAA
AGATCTTATTCGCACAGCAGTTGGTCAAACAAGACTCCTTATGAAGGAAAGGTTTAAACA
GTTTGAAGGACTGGTTGATGATTGTGAATATAAACGAGGTATAAAGGAGACTACCTGTAC
AGATCTGGATGGATTTTGGGATATGGTTAGTTTTCAGATAGAAGATGTAATCCACAAATT
CAACAATCTGATCAAACTTGAGGAATCTGGGTGGCAAGTCAATAATAATATGAATCATAA
TATGAACAAAAATGTCTTTAGGAAAAAAGTTGTCTCAGGTATAGCAAGTAAACCAAAACA
GGATGATGCTGGAAGAATTGCAGCGAGAAATCGCCTAGCTGCCATAAAAAATGCAATGAG
AGAGAGAATTAGGCAGGAAGAATGTGCTGAAACAGCAGTTTCTGTGATACCAAAGGAAGT
TGATAAAATAGTGTTCGATGCTGGATTTTTCAGAGTTGAAAGTCCTGTTAAATTATTCTC
AGGACTTTCTGTCTCTTCTGAAGGCCCTTCTCAAAGACTTGGAACACCTAAGTCTGTCAA
CAAAGCTGTATCTCAGAGTAGAAATGAGATGGGCATTCCACAACAAACTACATCACCAGA
AAATGCCGGTCCTCAGAATACGAAAAGTGAACATGTGAAGAAGACTTTGTTTTTGAGTAT
TCCTGAAAGCAGGAGCAGCATAGAAGATGCTCAGTGTCCTGGATTACCAGATTTAATTGA
AGAAAATCATGTTGTAAATAAGACAGACTTGAAGGTGGATTGTTTATCCAGTGAGAGAAT
GAGTTTGCCTCTTCTTGCTGGTGGAGTAGCAGATGATATTAATACTAACAAAAAAGAAGG
AATTTCAGATGTTGTGGAAGGAATGGAACTGAATTCTTCAATTACATCACAGGATGTTTT
GATGAGTAGCCCTGAAAAAAATACAGCTTCACAAAATAGCATCTTAGAAGAAGGGGAAAC
TAAAATTTCTCAGTCAGAACTATTTGATAATAAAAGTCTCACTACTGAATGCCACCTTCT
TGATTCACCAGGTCTAAACTGCAGTAATCCATTTACTCAGCTGGAGAGGAGACATCAAGA
ACATGCCAGACACATTTCTTTTGGTGGTAACCTGATTACTTTTTCACCTCTACAACCAGG
AGAATTTTGAATTTAAAAATAAATCCAAACATTTTCCTTCATATTATCAATGCTTATATA
TTCCTTAGACTATTGAAATTTTGGAGAAAATGTATTTGTGTTCACTTCTATAGCATATAA
TGTTTTAATATTCTGTGTTCATCAAAGTGTATTTTAGATATACTCTTTCTCAAGGGAAGT
GGGGATATTTTGTACATTTTCAACACAGAATAAAAAATGTACTGTGCCTTGAAAAAAAAA
AAAAAA
Protein sequence predicted based on nucleotide sequence
MKTILGDQRKQMLQKYKEEKQLQKLKEQREKAKRGIFKVGRYRPDMPCFLLSNQNAVKAE PKKAIPSSVRITRSKAKDQMEQTKIDNESDVRAIRPGPRQTSEKKVSDKEKKVVQPVMPT SLRMTRSATQAAKQVPRTVSSTTARKPVTRAANENEPEGKVPSKGRPAKNVETKPDKGIS CKVDSEENTLNSQTNATSGMNPDGVLSKMENLPEINTAKIKGKNSFAPKDFMFQPLDGLK TYQVTPMTPRSANAFLTPSYTWTPLKTEVDESQATKEILAQKCKTYSTKTIQQDSNKLPC PLGPLTVWHEEHVLNKNEATTKNLNGLPIKEVPSLERNEGRIAQPHHGVPYFRNILQSET EKLTSHCFEWDRKLELDIPDDAKDLIRTAVGQTRLLMKERFKQFEGLVDDCEYKRGIKET TCTDLDGFWDMVSFQIEDVIHKFNNLIKLEESGWQVNNNMNHNMNKNVFRKKVVSGIASK PKQDDAGRIAARNRLAAIKNAMRERIRQEECAETAVSVIPKEVDKIVFDAGFFRVESPVK LFSGLSVSSEGPSQRLGTPKSVNKAVSQSRNEMGIPQQTTSPENAGPQNTKSEHVKKTLF LSIPESRSSIEDAQCPGLPDLIEENHVVNKTDLKVDCLSSERMSLPLLAGGVADDINTNK KEGISDVVEGMELNSSITSQDVLMSSPEKNTASQNSILEEGETKISQSELFDNKSLTTEC HLLDSPGLNCSNPFTQLERRHQEHARHISFGGNLITFSPLQPGEF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
