Gene:
Gene Symbol:
MCF2L
Gene Name:
MCF.2 cell line derived transforming sequence-like
Original Clone ID:
ccsbBroadEn_11708
Keyword:
PERFECT_MATCH
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00623008
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACGGTGCGCCGGCTGTCACTGCTGTGCCGGGACCTCTGGGCGCTGTGGCTGCTGCTG
AAGGCCGGCGCAGATGAAATCATGCACCAGGACATCGTCCCGCTCTGTGCTGCCGACATC
CAGGACCAGCTAAAGAAGCGCTTTGCTTACCTGTCCGGTGGGCGGGGGCAGGACGGAAGC
CCGGTTATCACCTTCCCTGACTACCCGGCCTTCAGCGAGATTCCGGACAAGGAGTTCCAG
AATGTCATGACCTACCTCACCAGCATCCCCAGCCTGCAGGACGCTGGCATCGGATTCATC
CTGGTGATAGACCGGCGACGGGACAAATGGACCTCCGTGAAGGCGTCCGTCCTGCGCATC
GCAGCATCTTTCCCGGCAAACCTGCAGCTCGTCCTCGTGCTTCGCCCGACGGGTTTTTTC
CAAAGGACTCTCTCCGACATCGCTTTCAAATTCAATAGAGATGACTTTAAGATGAAGGTG
CCGGTCATAATGCTGAGCTCCGTACCAGACTTACACGGTTACATCGATAAGTCGCAGCTG
ACCGAGGACCTGGGTGGGACCCTGGACTACTGCCACTCCCGGTGGCTGTGCCAGCGCACG
GCCATCGAAAGTTTCGCCCTCATGGTGAAGCAGACGGCTCAGATGCTGCAGTCCTTCGGG
ACCGAGCTGGCTGAAACAGAGCTGCCCAATGACGTCCAGTCGACAAGCTCAGTGCTGTGT
GCGCACACAGAGAAGAAGGACAAGGCGAAGGAGGATTTGAGGCTGGCACTGAAAGAGGGG
CACAGTGTCCTGGAGAGCCTCAGGGAGCTGCAGGCTGAGGGCTCAGAGCCCAGTGTGAAC
CAGGACCAGCTTGACAACCAGGCCACCGTGCAGAGGCTCCTGGCCCAGCTGAACGAAACC
GAGGCTGCCTTCGATGAGTTCTGGGCAAAGCATCAGCAGAAACTGGAGCAGTGTCTGCAG
CTCCGGCACTTTGAGCAGGGCTTCCGGGAGGTCAAAGCCATCTTGGACGCAGCGTCCCAG
AAGATAGCAACCTTCACAGACATCGGCAACAGCCTGGCGCATGTGGAGCACCTGCTGAGG
GACCTGGCCAGCTTCGAGGAGAAATCAGGCGTGGCCGTGGAGAGGGCCCGGGCCCTGTCT
CTGGACGGCGAGCAGCTCATTGGGAACAAGCACTACGCGGTAGACTCCATCCGCCCAAAG
TGCCAGGAGCTCCGGCACCTCTGTGACCAGTTCTCTGCGGAGATCGCAAGGAGGAGGGGG
CTGCTCAGCAAGTCCCTGGAGCTGCACCGCCGCCTGGAGACGTCCATGAAGTGGTGTGAT
GAAGGGATTTACCTGCTGGCCTCACAACCTGTGGACAAGTGCCAGTCCCAGGACGGCGCG
GAGGCTGCCCTCCAGGAAATCGAGAAGTTTTTGGAGACCGGTGCGGAAAATAAGATCCAG
GAGCTCAACGCGATTTACAAGGAATACGAATCCATCCTCAACCAAGATCTCATGGAGCAC
GTGCGAAAGGTCTTCCAGAAGCAGGCAAGCATGGAGGAGGTGTTCCACCGCAGGCAGGCC
AGCCTGAAGAAGCTGGCGGCCAGGCAGACGCGGCCCGTGCAGCCGGTGGCCCCCAGACCC
GAGGCACTGGCAAAGTCGCCCTGCCCCTCCCCAGGCATTCGGCGAGGCTCTGAGAACTCC
AGCTCCGAGGGCGGTGCGCTCCGGAGAGGGCCCTACCGGAGGGCCAAGAGTGAGATGAGT
GAGAGCCGGCAGGGCCGCGGCTCAGCGGGGGAGGAGGAGGAAAGCCTGGCCATCCTGCGC
AGGCACGTGATGAGCGAGCTCCTGGACACAGAACGGGCCTACGTGGAGGAGCTGCTGTGC
GTCCTGGAGGGCTACGCCGCGGAGATGGATAACCCACTGATGGCTCACCTCCTGTCAACA
GGCCTTCACAACAAGAAGGATGTTTTGTTTGGAAACATGGAGGAAATCTATCACTTCCAC
AACAGGATATTCCTCAGGGAGCTGGAAAACTACACTGACTGCCCAGAACTGGTTGGAAGA
TGCTTTCTGGAGAGGATGGAAGATTTCCAGATCTATGAGAAGTACTGTCAGAACAAGCCC
CGCTCTGAGAGCCTGTGGAGACAGTGCTCCGACTGCCCGTTTTTCCAGGAATGCCAGAGA
AAGCTGGACCACAAGCTGAGCCTGGACTCCTACCTGCTGAAGCCAGTGCAGAGGATCACC
AAGTACCAGCTGCTGCTCAAGGAAATGCTGAAATACAGCAGGAACTGCGAGGGGGCTGAG
GACCTGCAGGAGGCGCTGAGCTCCATCCTGGGCATCCTGAAGGCCGTGAACGACTCCATG
CACCTCATCGCTATCACCGGCTATGACGGGAATCTCGGCGACCTGGGCAAGCTGCTGATG
CAGGGCTCGTTCAGCGTCTGGACCGACCACAAGAGGGGCCACACCAAGGTGAAGGAGCTG
GCCAGGTTCAAGCCCATGCAGCGGCACCTGTTCCTGCACGAGAAGGCAGTGCTCTTCTGC
AAGAAGAGGGAGGAGAATGGGGAGGGGTATGAGAAAGCTCCCTCCTACAGCTACAAGCAG
TCCTTAAACATGGCTGCCGTTGGCATTACGGAGAACGTGAAGGGAGATGCTAAGAAGTTC
GAGATCTGGTACAACGCGCGCGAGGAGGTCTACATCGTCCAGGCGCCAACTCCTGAGATT
AAAGCCGCGTGGGTGAATGAAATTCGGAAAGTGCTGACCAGCCAGCTGCAGGCTTGTAGA
GAAGCCAGCCAGCACCGGGCGCTGGAGCAGTCACAGAGCCTGCCCCTGCCGGCCCCGACC
AGCACCAGTCCCTCAAGAGGAAACTCAAGGAACATCAAGAAGCTGGAAGAAAGGAAAACA
GACCCCCTAAGCCTGGAGGGATACGTCAGCTCAGCGCCACTGACAAAGCCCCCCGAAAAG
GGCAAAGAGCCCTAG
Protein sequence predicted based on nucleotide sequence
MTVRRLSLLCRDLWALWLLLKAGADEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGS PVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRI AASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQL TEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLC AHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNET EAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLR DLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRG LLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQ ELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRP EALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILR RHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFH NRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQR KLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSM HLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFC KKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEI KAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKT DPLSLEGYVSSAPLTKPPEKGKEP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
